Patents Assigned to Danisco US Inc.
  • Trichoderma reesei glucoamylase variants resistant to oxidation-related activity loss and the use thereof
    Patent number: 9540669
    Abstract: Embodiments of the present disclosure relate to Trichoderma reesei glucoamylase (TrGA) variants having improved properties (e.g., improved thermostability, improved specific activity, and/or resistant to oxidation-related activity loss). Also provided are compositions comprising variant glucoamylases. These compositions are useful in various starch process applications.
    Type: Grant
    Filed: August 12, 2013
    Date of Patent: January 10, 2017
    Assignee: Danisco US Inc.
    Inventors: Su Yin Marina Chow, Thomas P. Graycar, Jacquelyn A. Huitink, Casper Vroemen, David L. Wong
  • Method for amplifying locus in bacterial cell
    Patent number: 9528114
    Abstract: Certain aspects of this disclosure relate to a method of amplifying a genomic locus. In certain embodiments, the method may comprise: a) contacting a population of bacterial host cells with an inhibitor of an essential enzyme, where the bacterial host cells comprise a genomic locus of the structure: A1-P-M-A2, where A1 and A2 are direct repeats, P comprises a coding sequence for a polypeptide, and M comprises a coding sequence for the essential enzyme; and b) selecting for cells that are resistant to the inhibitor; wherein cells that are resistant to the inhibitor have multiple copies of the amplification unit.
    Type: Grant
    Filed: March 27, 2009
    Date of Patent: December 27, 2016
    Assignee: DANISCO US INC.
    Inventors: Eugenio Ferrari, Caroline M. Peres
  • Cellulase variants with improved expression, activity and stability, and use thereof
    Patent number: 9523087
    Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.
    Type: Grant
    Filed: February 25, 2014
    Date of Patent: December 20, 2016
    Assignee: DANISCO US INC
    Inventors: Wolfgang Aehle, Richard R. Bott, Benjamin S. Bower, Jonathan Caspi, David A. Estell, Frits Goedegebuur, Ronaldus Wilhelmus Joannes Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Johannes Franciscus Thomas Van Lieshout, Igor Nikolaev, Sander Van Stigt Thans, Louise Wallace, Gudrun Vogtentanz, Mats Sandgren
  • Modified proteases
    Patent number: 9523088
    Abstract: This invention relates to modified polynucleotides encoding modified proteases, and methods for altering the production of proteases in microorganisms. In particular, the present invention relates to methods for altering the expression of proteases in microorganisms, such as Bacillus species. The invention discloses modified polynucleotides, vectors, modified polypeptides, and processes for enhancing the production of proteases.
    Type: Grant
    Filed: June 16, 2014
    Date of Patent: December 20, 2016
    Assignee: DANISCO US INC.
    Inventors: Eugenio Ferrari, David A. Estell
  • Methods for Improving By-Products from Fermentation Processes Using Xylanase
    Publication number: 20160333332
    Abstract: Provided are GH10 xylanases or a fragment thereof including modified xlyanase enzymes, all having xylanase activity, wherein the enzyme or fragment thereof provides increased oil recovery from a grain-based material compared with a control or parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at at least one or more of the following positions 7, 25, 33, 64, 79, 89, 217 and 298, wherein the numbering is based on the amino acid numbering of FveXyn4 (SEQ ID No. 1). Methods of using the GH10 xylanases, for example, for improved oil recovery are also provided.
    Type: Application
    Filed: January 15, 2015
    Publication date: November 17, 2016
    Applicant: Danisco US Inc.
    Inventors: Paula Johanna Maria Teunissen, Michael J. Pepsin, Vivek Sharma
  • Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene
    Patent number: 9493791
    Abstract: The invention provides for methods for the production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in cells via the heterologous expression of phosphoketolase enzymes.
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: November 15, 2016
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Zachary Q. Beck, Andrew C. Eliot, Caroline M. Peres, Dmitrii V. Vaviline
  • SERINE PROTEASES OF THE BACILLUS GIBSONII-CLADE
    Publication number: 20160319266
    Abstract: The present disclosure relates to serine proteases cloned from Bacillus gibsonii, and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.
    Type: Application
    Filed: December 12, 2014
    Publication date: November 3, 2016
    Applicant: Danisco US Inc.
    Inventors: Marc Kolkman, Rie Mejldal, Frits Goedegebuur, Lilia Maria Babe, Anja Hemmingsen Kellett-Smith, Harm Mulder, Richard R. Bott, Miles Christopher Scotcher
  • ENHANCED PROTEIN EXPRESSION
    Publication number: 20160319288
    Abstract: The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include Gram positive microorganisms, such as Bacillus species, having a genetic alteration that modifies activity of a protein encoded by the ykf operon and results in enhanced expression of a protein of interest.
    Type: Application
    Filed: December 8, 2014
    Publication date: November 3, 2016
    Applicant: DANISCO US INC.
    Inventors: Cristina BONGIORNI, Rei OTSUKA, Brian F. SCHMIDT, Anita VAN KIMMENADE
  • SERINE PROTEASES OF BACILLUS SPECIES
    Publication number: 20160312204
    Abstract: The present disclosure relates to serine proteases cloned from Bacillus akibai and Bacillus clarkii, and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.
    Type: Application
    Filed: December 12, 2014
    Publication date: October 27, 2016
    Applicant: Danisco US Inc.
    Inventors: Marc Kolkman, Rie Mejldal, Anja Hemmingsen Kellett-Smith, Lilia Maria Babe, Richard R. Bott
  • USE OF HYDROPHOBINS TO INCREASE GAS TRANSFER IN AEROBIC FERMENTATION PROCESSES
    Publication number: 20160312178
    Abstract: The present disclosure provides methods, compositions and apparatuses for increasing gas transfer in fermentation processes.
    Type: Application
    Filed: November 14, 2014
    Publication date: October 27, 2016
    Applicant: DANISCO US INC.
    Inventors: Evan SIMS, Michael W. SCHELLE, Gopal CHOTANI
  • Increased isoprene production using the archaeal lower mevalonate pathway
    Patent number: 9464301
    Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
    Type: Grant
    Filed: July 3, 2014
    Date of Patent: October 11, 2016
    Assignees: DANISCO US INC., THE GOODYEAR TIRE & RUBBER COMPANY
    Inventors: Zachary Q. Beck, Anthony R. Calabria, Michael C. Miller, Alex T. Nielsen, Dmitrii V. Vaviline
  • Stable Enzymes by Glycation Reduction
    Publication number: 20160289653
    Abstract: The present teachings provide enzymes with improved stability arising from reduced glycation. Glycation reduction can be achieved by a variety of means, including protein engineering, heating to remove background ground starch hydrolytic activity, diafiltration afiltration, and various formulation approaches including non-inclusion of problematic sugars, and inclusion of resistant starches.
    Type: Application
    Filed: November 14, 2014
    Publication date: October 6, 2016
    Applicant: Danisco US Inc.
    Inventors: Mark S. Gebert, Soren Dalsgaard, Mariliz Ortiz Johnson, Adam L. Garske, Douglas A. Dale
  • VARIANT ALPHA-AMYLASES HAVING REDUCED SUSCEPTIBILITY TO PROTEASE CLEAVAGE, AND METHODS OF USE, THEREOF
    Publication number: 20160272957
    Abstract: Disclosed are compositions and methods relating to variant alpha-amylase enzymes comprising mutations that reduce their susceptibility to proteolytic digestion and improve their performance. The variants alpha-amylases are particularly useful in application that require alpha-amylases and proteases to be present in the same solution.
    Type: Application
    Filed: November 13, 2014
    Publication date: September 22, 2016
    Applicant: Danisco US Inc.
    Inventor: Dina Finan
  • Glucoamylase Variants
    Publication number: 20160272958
    Abstract: Aspects of the present disclosure include glucoamylase (GA) variants having at least one improved property over a parent GA, compositions containing the GA variants, nucleic acids encoding the GA variants, and methods for producing and using the same. In some aspects, the GA variant is a variant of a parent GA from Humicola grisea.
    Type: Application
    Filed: December 3, 2014
    Publication date: September 22, 2016
    Applicant: Danisco US Inc.
    Inventors: Daniel Esteban Torres Pazmino, Viktor Yuryevich Alekseyev, Casper Willem Vroemen, David A. Estell
  • VARIANTS OF CELLOBIOHYDROLASES
    Publication number: 20160272959
    Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
    Type: Application
    Filed: December 10, 2013
    Publication date: September 22, 2016
    Applicant: Danisco US Inc.
    Inventors: Richard R. BOTT, Maria FOUKARAKI, Ronaldus Wilhelmus HOMMES, Thijs KAPER, Bradley R. KELEMEN, Slavko KRALJ, Igor NIKOLAEV, Mats SANDGREN, Johannes Franciscus Thomas VAN LIESHOUT, SANDER VAN STIGT THANS
  • Beta-glucosidase I variants with improved properties
    Patent number: 9447400
    Abstract: The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants.
    Type: Grant
    Filed: November 19, 2010
    Date of Patent: September 20, 2016
    Assignee: DANISCO US INC.
    Inventors: Richard R. Bott, Thijs Kaper, Bradley Kelemen, Frits Goedegebuur, Ronaldus Wilhelmus Hommes, Slavko Kralj, Paulien Kruithof, Igor Nikolaev, Wilhelmus Antonious Hendricus Van Der Kley, Johannes Franciscus Thomas Van Lieshout, Sander Van Stigt Thans, Gudrun Vogtentanz, Mats Sandgren
  • Glucoamylase variants with altered properties
    Patent number: 9447397
    Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: October 9, 2007
    Date of Patent: September 20, 2016
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Piet Van Solingen, Martijn S. Scheffers, Richard R. Bott
  • Large Scale Genetically Engineered Active Dry Yeast
    Publication number: 20160264927
    Abstract: The present teachings provide large scale genetically engineered yeast in active dry form.
    Type: Application
    Filed: October 27, 2014
    Publication date: September 15, 2016
    Applicant: Danisco US Inc.
    Inventors: Andrei Miasnikov, Douglas A. Dale, Douglas Ko, George England, Wyatt C. Smith, Beth Fryksdale, Matthew T. Reboli
  • RECOVERY OF INSOLUBLE ENZYME FROM FERMENTATION BROTH AND FORMULATION OF INSOLUBLE ENZYME
    Publication number: 20160264956
    Abstract: Methods are provided for recovery and formulation of insoluble enzymes from a microbial fermentation broth, without removal of microbial cells or cell debris. Granular and liquid formulations comprising insoluble enzymes are also provided.
    Type: Application
    Filed: March 21, 2016
    Publication date: September 15, 2016
    Applicant: Danisco US Inc.
    Inventors: Michael Bodo, Robert I. Christensen, Rajdeep S. Dhaliwal, Meng H. Heng
  • Enhanced Fermentation Process Using a Transglycosidase
    Publication number: 20160257977
    Abstract: The present invention relates to methods for improving fermentation processes, including increasing product yield, reduced viscosity, and/or reduced foaming.
    Type: Application
    Filed: October 21, 2014
    Publication date: September 8, 2016
    Applicant: Danisco US Inc.
    Inventors: Gang Duan, Jayarama K. Shetty, Xin Wang, Hongxian Xu, Xiaoping Zhang, Peng Zhou