Abstract: A method, and also a gas supply system without a separate humidifying apparatus, for supplying gas to a plurality of bioreactors, divides a constant gas stream with high distribution accuracy into a plurality of gas substreams having a mandated volume flow, which can be kept constant at the mandated level even when during gas supply there is fluctuation in the opposing pressure in the gas line to the respective bioreactor, and decouples a gas distribution from the opposing pressure by hydrostatic pressure compensation, with the gas distribution at the same time producing an obligatory humidification of the gas stream.

Abstract: The disclosure relates to a composting device including a housing defining an interior, and having a top wall defining an access opening providing access to the interior, a door moveable relative to the access opening between open/closed positions to selectively open/close the access opening, a trim bezel with a trim bezel opening, and a bin located within the interior and having a fill opening in communication with the trim bezel opening.

Abstract: Provided is a cell culture device that has a simple structure and ensures sufficient nutrient supply to cells and oxygenation of the cells to thereby enable mass cell culture. The cell culture device comprises: a cell culture container which is an approximately cylindrical body provided with a flat bottom part at the lower end; a dish-shaped body having an approximately disc shape which is provided with a plurality of magnetic attraction members, said magnetic attraction members being positioned in the circumferential part at equal intervals, and horizontally disposed in a non-contact state within a hollow space inside the cell culture container; and a cyclic body which is provided with a plurality of magnetic attraction members and positioned outside the cell culture container so that the cell culture container is located within the cycle thereof. The cyclic body and the dish-shaped body move vertically in magnetic conjunction to thereby agitate a medium and supply nutrients to cells.

Abstract: A system for incubating one or more cells and/or organotypic cultures for biological investigation, in particular for toxicology assessment, comprising a bio-incubator and a pressure system fluidly connected with the bio-incubator. The pressure system is a cyclic gas pressure system configured for cyclically varying the gas pressure in the bio-incubator between a negative pressure and a positive pressure compared to the atmospheric pressure, so as to reproduce the pressure conditions in lungs of a living mammal. The system is remarkable in that the cyclic gas pressure system comprises a feedthrough with a pipe configured to deviate the air influx from the one or more cells and/or organotypic cultures.

Abstract: The invention features devices and kits for capturing and culturing microorganisms (e.g., bacteria, fungi, or protists) and methods of using the devices and kits to detect microorganisms in environmental and other samples. The device includes a nutrient media having a flat growth area on which microorganisms can grow. Samples are collected by contacting the device with any environmental sample, e.g., rolling device on a work surface or exposing device to air, or by filtering a sample through a membrane. Microorganisms deposited on the membrane derive nutrients from the underlying media and grow into colonies that can then be detected using methods known in the art. The detected colonies can be imaged digitally or with film.

Abstract: The present disclosure relates to methods and apparatus for measuring of multiple physiological properties of biological samples, such as measuring biological flux.

Abstract: A method of vascularising a cell aggregate on a microfluidic device, microfluidic cell culture devices comprising perfusable vascular networks and kits and assays using the microfluidic cell culture devices are described. The microfluidic devices comprise one or more capillary pressure barriers allowing for formation of an extracellular matrix gel within a confined area of the network, in which cells can be cultured for different uses.

Abstract: A sample testing apparatus for characterizing at least one target molecule in a testing sample includes a substrate and at least one detection device over the substrate. Each detection device includes a plurality of electrodes, a plurality of data lines, and a probe. Each electrode is configured, upon reaction of the probe with one of the at least one target molecule, to sense an electrical signal, and then to transmit the electrical signal via the one data line. Each data line includes a first film layer and at least one other film layer disposed over the first film layer. The first film layer can be at a substantially same layer, and have a first composition substantially same, as the electrodes. One or more of the at least one other film layer can have a composition having a relatively lower electric resistance than the first composition.

Abstract: A device for retaining a biological sample for performing a cryoprocedure on a biological sample having a tubular member having a lumen extending therein, the lumen configured to receive the biological sample. A retainer is couplable to a distal end of the tubular member, the retainer having a perforated member having at least one orifice, the at least one orifice having a dimension smaller than a dimension of the at least one biological sample to prevent exit of the biological sample from the tubular member, wherein the perforated element is configured to allow inflow of liquids to communicate with the lumen containing the biological sample.

Abstract: A multi-organ repairing and transferring device without interrupting a blood flow includes a first perfusion bin, a second perfusion bin and a third perfusion bin which are configured for storing respective organs. A first pump is arranged on a first pipeline, two ends of the first pipeline are connected with the first perfusion bin and the second perfusion bin respectively, a second pump is arranged on a second pipeline, two ends of the second pipeline are connected with the first perfusion bin and the second perfusion bin respectively, a third pump is arranged on a third pipeline, two ends of the third pipeline are connected with the first perfusion bin and the third perfusion bin respectively, and a fourth pump is arranged on a fourth pipeline, two ends of the fourth pipeline are connected with the first perfusion bin and the third perfusion bin respectively.

Abstract: An anaerobic digestion device based on self-sustained air flotation includes an anaerobic digestion tank unit, a self-sustained air flotation screening unit and a biogas measurement and collection unit. The self-sustained air flotation screening unit includes an air flotation screening part, a material sedimentation part, a reflux part and a three-phase separation part connected sequentially from bottom to top. A digested material in the anaerobic digestion tank unit is pumped into the air flotation screening part, overflows into the material sedimentation part, and then is raised to the reflux part. Gas passing through the three-phase separation part and gas produced in the anaerobic digestion tank unit enter the biogas measurement and collection unit to be measured and collected.

Abstract: A sample testing apparatus includes a sample tray defining a planar surface and a plurality of wells recessed relative to the planar surface, and a lid member configured to be sealed about the planar surface of the sample tray. The lid member includes an adhesive layer configured to be sealed to the planar surface of the sample tray, a breathable film layer disposed about the adhesive layer, and a backing layer disposed about the breathable film layer. Methods of using the sample testing apparatus for testing a sample and kits to facilitate such testing are also provided.

Abstract: The invention discloses a device for producing biogas with high methane content by utilizing livestock and poultry feces, wherein the interior of a tank body of a biogas fermentation tank is divided by a baffle, so as to form a main reaction chamber and an auxiliary reaction chamber which are communicated in upper portions, so that a reactant flows into the auxiliary reaction chamber only after entering the main reaction chamber via a relatively low feeding hole and then reaching a high position of a liquid level, and extension of fermentation time is realized, meanwhile, scales formed at the top of fermentation broth flow into the auxiliary reaction chamber along with liquid, so that the interior of the main reaction chamber keeps a liquid state all the time, and sealing and reduction of quantity of anaerobic bacteria are avoided.

Abstract: The invention relates to a single-use connection device for insertion into a connection opening of a bioreactor, in particular in a connection opening of a top plate of a dimensionally-stable bioreactor or in a connection opening of a connection element of a pouch bioreactor. The single-use connection device is inserted in a connection opening of a top plate of a bioreactor and/or in a connection opening of a pouch reactor and is provided with multiple passages and an attachment section, wherein the single-use connection device is formed as a single piece and is made of plastic. The attachment section, on an outer peripheral surface, has an attachment structure for attaching the single-use connection device in the connection opening.

Abstract: An organ preservation system is provided. The organ preservation system is embodied in a sleeve adapted to maintain a pre-transplant organ at a temperature lower than ambient temperature through a plurality of bladders interconnected by a deformable mesh. With the organ in the sleeve, each vessel of the organ can pass through separate, deformable holes of the mesh, facilitating ease of identification of each vessel needed for the pending vascular anastomosis.

Abstract: Methods for identifying compounds that modulate cellular responses stimulated by IgE, which include providing an impedance-based system that monitors cell-substrate impedance of cells on a substrate; introducing cells to the substrate of the system; adding at least one test compound and IgE to the cells, wherein the at least one test compound is suspected of modulating cell responses stimulated by the IgE; adding an antigen to the cells; monitoring the cell-substrate impedance of cells on the substrate; and analyzing the cell-substrate impedance to evaluate whether the at least one test compound alters a cellular response to stimulation with the IgE.

Abstract: An automated cell culture and tissue engineering system comprising defined and separate environmental zones provide for increased control and maintenance of the internal environment of the system such that the temperature, air flow and gases surrounding the bioreactor module form one zone that is maintained separately to a second zone formed surrounding the reagent fluid reservoir. The system further comprises means for elimination and/or management of condensation within the second zone of the system.

Abstract: The disclosure relates to bioreactors, for example for biological treatment and, more specifically to bioreactor insert apparatus including biofilms and related methods. The bioreactor insert apparatus provides a means for circulation of reaction medium within the bioreactor, a biofilm support, and biological treatment of an inlet feed to the reactor/insert apparatus. The bioreactor insert apparatus has a high relative surface area for biofilm attachment and is capable of generating complex flow patterns and increasing treatment efficiency/biological conversion activity in a biologically-active reactor. The high surface area structure incorporates multiple biofilm support structures such as meshes at inlet and outlet portions of the structure. The biofilm support structures and biofilms thereon can increase overall reaction rate of the bioreactor and/or perform some solid/liquid separation in the treatment of the wastewater or other influent.

Abstract: Disclosed are devices and methods for non-cryogenic vitrification of biological materials that include the steps of providing one or more capillary channels of which a first opening is operably in contact with a moisture containing vitrification mixture made of a biological material and a vitrification agent.

Abstract: An object of the invention is to provide an electroporation method for treating vesicles with exogenous material for insertion of the exogenous material into the vesicles which includes the steps of: a. retaining a suspension of the vesicles and the exogenous material in a treatment volume in a chamber which includes electrodes, wherein the chamber has a geometric factor (cm?1) defined by the quotient of the electrode gap squared (cm2) divided by the chamber volume (cm3), wherein the geometric factor is less than or equal to 0.1 cm?1, wherein the suspension of the vesicles and the exogenous material is in a medium which is adjusted such that the medium has conductivity in a range spanning 50 microSiemens/cm to 500 microSiemens/cm, wherein the suspension is enclosed in the chamber during treatment, and b. treating the suspension enclosed in the chamber with one or more pulsed electric fields.