Abstract: A method for imaging is presented. The method includes acquiring a plurality of images corresponding to at least one field of view at a plurality of sample distances. Furthermore, the method includes determining a figure of merit corresponding to each pixel in each of the plurality of acquired images. The method also includes for each pixel in each of the plurality of acquired images identifying an image in the plurality of images that yields a best figure of merit for that pixel. Moreover, the method includes generating an array for each image in the plurality of images. In addition, the method includes populating the arrays based upon the determined best figures of merit to generate a set of populated arrays. Also, the method includes processing each populated array in the set of populated arrays using a bit mask to generate bit masked filtered arrays. Additionally, the method includes selecting pixels from each image in the plurality of images based upon the bit masked filtered arrays.

Abstract: A microscopy assembly comprises a stand, a stereo microscope supported by the stand, and a mouth switch assembly mounted to a holder, the mouth switch assembly including a mouth piece grippable by a user's teeth, and with a force sensor actuatable by the exertion of pressure by a user's lip while the mouth piece is held by the user's teeth, the force sensor including an actuating element arranged at a side of the mouth piece, wherein an actuating area of the actuating element located next to a front end of the mouth piece is spaced apart from the front end of the mouth piece by a distance larger than 4 mm and smaller than 35 mm.

Abstract: A microscope stand for holding an optical and/or opto-electronical (e.g. video) observation system is mechanically connected to a microscope base, such as a basis plate, feet or rack or the like. In at least one embodiment, the microscope stand is made in two parts, the first part being provided for holding the observation system, and the second part is substantially positively connected to the first part. This second part includes at least one, preferably shaft-like, cavity, in which at least one electric or electronic component, which is preferably either an electric illumination system for the object to be examined by way of the observation system (in this respect the illumination system forms part of the observation system) and/or of an electronic camera system.

Abstract: A microscope comprising a main objective including a lens assembly movable in the direction of the optical axis of the main objective for focal length variation and comprising an illuminating unit with an illumination deflector element for generating an illuminating beam path directed onto an object plane and extending outside the main objective. The position of the illumination deflector element is adjustable dependent on a focal length variation of the main objective for centering the illumination. The illumination deflector element is movable in a direction parallel to the optical axis of the main objective and is coupled to the movable lens assembly of the main objective.

Abstract: A laser scanning microscope scans a plurality of scanning areas based on a drive table to indicate time series data for driving a scanner used for performing scanning with laser light. The laser scanning microscope includes a drive table creation unit and a drive control unit. The drive table creation unit is applied for creating an interpolating drive table for specifying a scanning path between an end point of a first scanning area and a start point of a second scanning area which is scanned next to the first scanning area. The drive control unit is applied for controlling driving of the scanner based on the interpolating drive table between the first scanning area and the second scanning area.

Abstract: The invention relates to a lens or lens attachment component, which is designed to be mounted in a microscope and to which an electronic memory module (15) is fixed. Said component comprises two contact fields (16, 17; 18, 19) that are electrically connected to connections of the memory module (15), said fields permitting the memory module (15) to be electrically contacted and supplied with energy once the component is mounted.

Abstract: A microscope includes an observation optical unit that has a zoom optical system having variable zoom power and an objective lens optically connected to the zoom optical system, the observation optical unit receiving observation light emitted from an observation sample, a focusing unit that has a focusing mechanism to focus the observation optical unit on the observation sample, a light source unit that emits illuminating light to illuminate the observation sample, and an illuminating unit that has a reflecting optical element disposed in the observation optical unit but out of an optical path of the zoom optical system, the illuminating unit being partially disposed in the focusing unit, the illuminating unit illuminating the observation sample with the illuminating light via the reflecting optical element and the objective lens.

Abstract: The invention relates to a microscope objective with preferably anti-symmetric lenses or lens groups with an optical magnification of ?100 and a visual field factor of 20. According to the invention the microscope objective consists of 9 lenses with 3 cemented elements, starting from the object side (left), a lens which is almost a hemisphere L1 with positive refractive power, a meniscus lens L2 with positive refractive power, a two-part cemented element G1 with positive refractive power, another two-art cemented element G2 with positive refractive power, a two-part cemented element G3 with negative refractive power, and finally a meniscus lens L9 with negative refractive power. By using cemented elements and lens pairings of the same construction, production costs can be reduced compared with methods of the prior art while image contrast is improved.

Abstract: It is possible to use the magnification of an objective lens as is, substantially without changing the observation magnification, even when the working distance of the objective lens is changed. The invention provides a focus-adjusting unit disposed on an optical axis between an objective optical system that collects light from a specimen and an image-forming optical system that images the light collected by the objective optical system at a prescribed position, comprising a front optical system and a back optical system, sequentially disposed along the optical axis direction from a front side, with the objective optical system serving at the front side and the image-forming optical system at the back; and a lens driving part that relatively moves these optical systems in the optical axis direction, wherein the front optical system and the back optical system have refractive powers of different sign and focal lengths of substantially equal absolute value.

Abstract: A bottomless micro-mirror well. In one embodiment, the bottomless micro-mirror well includes a substrate having a first surface and an opposite, second surface defining a body portion between the first surface and the opposite, second surface, where the body portion defines an inverted pyramidal well having at least three side surfaces extending to each other and defining an opening between a first sidewall and a second sidewall of the body portion, and where each of the at least three side surfaces is configured to reflect light emitting from an object of interest.

Abstract: A clear fluorescence image is obtained by preventing external light from mixing with weak fluorescence emitted from a specimen, while reducing the size of the overall apparatus. The invention provides a fluorescence microscope apparatus including a stage for mounting a specimen; an objective lens for collecting fluorescence from the specimen; a moving mechanism for relatively moving the stage and the objective lens; a first cover member secured to the objective lens; a second cover member, provided at the stage, for enclosing a tip of the objective lens and a space above the stage, together with the first cover member; and a light-blocking member allowing relative movement of the first and second cover members while preventing leakage of light from a gap between the two cover members.

Abstract: A microscope having a mechanically adjustable zoom system (7) and/or a mechanically adjustable focus system is described, which microscope is equipped at least one manually movable adjusting element (2) for adjusting the zoom system (7) and/or the focus system. The adjusting element (2) has associated with it a sensor (1) for ascertaining and/or indicating the position of the adjusting element (2).

Abstract: Fluorescence is generated from an irradiated point on an inspection surface of a sample and the fluorescence is collected by an objective lens. Here, because of the magnification chromatic aberration of the objective lens, the fluorescence going out from the objective lens travels along a path shifted from the irradiation light and changed substantially into a non-scan light by a galvano-scanner. The fluorescence passes through a dichroic mirror into a deflection system after light of unnecessary wavelength is removed by a filter. The deflection system is driven in synchronization with the galvano-scanner by a computer and corrects the shift and inclination of the optical axis generated by the magnification chromatic aberration of the objective lens. Then, the fluorescence forms an image of the irradiation point of the inspection surface of the sample on a pin hole of a pin hole plate by using a collective lens.

Abstract: A movable part to which a specimen or optical component is fixed is effectively damped in a short period of time at low cost, while saving space and energy and avoiding heat generation, thus allowing high-precision observation of a specimen to be carried out quickly after stopping at a certain position. The invention provides a microscope system including a driving mechanism for driving a movable part to which a specimen or optical component is fixed and stopping the movable part at a certain position; and a vibration damping mechanism for damping vibrations generated when stopping the movable part with the driving mechanism. The vibration damping mechanism includes an inertial member having a prescribed mass and a viscoelastic member sandwiched between the inertial member and the movable part. Dimensions in a plurality of directions of the viscoelastic member are set on the basis of resonance frequencies of vibrations generated in multiple directions when stopping the movable part.

Abstract: An optical system includes a dynamically focused lens that variably changes focal length to focus light on a material under evaluation at discrete axial depths, and a scanning element that laterally scans the material to focus light on the material at points that are laterally adjacent the axial depth.

Abstract: An optical module for increasing magnification of a microscope includes an objective and an eyepiece between which is an optical path for light to travel. The optical includes light diverging elements for dividing the optical path into two parallel light paths after leaving the objective; and several orthonormally-arranged reflective elements including at least a first and a last reflective element, each reflective element having a reflective surface diagonal relative to the orthonormal arrangement of the elements, and also arranged to successively reflect the parallel light paths, from element to element, from the objective when the module is inserted in the optical microscope, beneath the eyepiece. Also included are light converging elements for converging the parallel paths into a single path before producing an image at the eyepiece.

Abstract: A stereomicroscope (20) has first and second main beam paths (21, 22) whose spacing defines a stereo base (23), a microscope axis (24) proceeding through the center of the stereo base (23) parallel to the main beam paths; and an optical beam splitter device (30) for generating an assistant's beam path (31) and a documentation beam path (32), the assistant's beam path being outcoupled from the first main beam path in a first position of the beam splitter device, and from the second main beam path in a second position, and the documentation beam path being outcoupled from the other main beam path; and the direction of the assistant's beam path in the first position being rotated 180° relative to the assistant's beam path in the second position; and the outcoupled documentation beam path extending, in both positions of the beam splitter device, perpendicular to the outcoupled assistant's beam path.

Abstract: For a confocal scanning microscope (1) an optical zoom system (41) with linear scanning is provided, which not only makes a zoom function possible, in that a variable magnification of an image is possible, but rather which additionally produces a pupil image in the illuminating beam path (IB) [BS] and thereby makes a variable image length possible (distance between the original pupil (En.P) [EP] and the imaged/reproduced pupil (Ex.P) [AP]) so that axially varying objective pupil positions can thereby be compensated.

Abstract: A surgical microscope (100) has an illuminating module (120). The illuminating module contains an illuminating optic which images a field diaphragm (124) to a parallel illuminating beam path at infinity. The field diaphragm (124) is illuminated by a light source. The illuminating optic includes a first lens assembly and a second lens assembly which functions to image the field diaphragm (124) into the object region (108) via the microscope main objective (101) of the surgical microscope (100). An in-coupling element (128) is provided between the first lens assembly (125) and the second lens assembly (126) and this in-coupling element couples the OCT-scanning beam into the illuminating beam.

Abstract: An optoelectronic detector and method of using same. In order to avoid any condensation on a surface, it has been known to heat such a surface. However, heating an optoelectronic detector will create a stronger hissing noise due to the greater dark current that is caused thereby. The invention is intended to avoid any condensation on an optoelectronic detector without airtight encapsulation. To this end, the detector is cooled and equipped with a sensor for the determination of a current value of one of the variables ambient humidity and ambient dew point temperature and a control unit that is connected with the sensor and designed to control the cooling device in dependence of such a value. By taking into account the ambient humidity or, respectively, the dew point temperature in the control of the cooling device, condensation on the detector can be avoided. An airtight encapsulation of the detector and the cooling device is not required.

Abstract: A fluorescence microscope 11 includes an objective lens 101, a dichroic mirror 102, a half mirror 105, a mirror 106, a laser light source 111, an ND filter 112, a beam expander 113, a mirror 114, a spatial light modulator 115, a lens 131, a band pass filter 132, a spatial light modulator 133, and a detector, etc. The spatial light modulator 115 can vary its spatial light modulation, and can set the number, positions, and shapes of regions to be irradiated with excitation light in the determined specimen 1 by irradiating the determined specimen 1 with spatially modulated excitation light via the subsequent optical system.

Abstract: An image acquiring apparatus for acquiring images of a sample includes a macro image acquiring unit 20 for acquiring a macro image of the sample, a dark field light source 26 to be used for acquiring a dark field macro image of the sample as a macro image, a macro image processing unit 66 which generates a reference macro image by processing image data of the macro image, and an image pickup condition setting unit 65 which sets an image acquiring range corresponding to a range including an object of image acquisition as an image pickup condition of a micro image of the sample by referring to the reference macro image. This realizes an image acquiring apparatus, an image acquiring method, and an image acquiring program by which a macro image of a sample as an object of image acquisition is preferably acquired.

Abstract: The size and production costs of an optical microscope apparatus capable of blocking light or maintaining the specimen environment are reduced. The provided optical microscope apparatus includes a microscope that has a stage for mounting a specimen (A), a transmission-illumination optical system, and a detection optical system; and a housing that surrounds the microscope, wherein the housing includes a fixed housing, and a movable housing, wherein, among optical parts constituting the transmission-illumination optical system and the image-forming optical system, at least some optical parts disposed above the stage are movable, and wherein a switching mechanism is provided, the switching mechanism being configured to retract the optical parts away from above the stage when the movable housing is disposed in an open position relative to the fixed housing and to substantially align the optical axes of both optical systems when the movable housing is disposed in a closed position relative to the fixed housing.

Abstract: A microscopy system is configured for creating 3D images from individually localized probe molecules. The microscopy system includes a sample stage, an activation light source, a readout light source, a beam splitting device, at least one camera, and a controller. The activation light source activates probes of at least one probe subset of photo-sensitive luminescent probes, and the readout light source causes luminescence light from the activated probes. The beam splitting device splits the luminescence light into at least two paths to create at least two detection planes that correspond to the same or different number of object planes of the sample. The camera detects simultaneously the at least two detection planes, the number of object planes being represented in the camera by the same number of recorded regions of interest. The controller is programmable to combine a signal from the regions of interest into a 3D data.

Abstract: The present invention relates to a microscope (1), preferably a dental microscope, including a microscope body (15) and a stand (2) formed by a plurality of components to provide a supporting function or to enable positioning of the microscope (1) in the room (11), the microscope body (15) and the stand (2) having cavities (16) therein. It is a feature of the present invention that at least one cavity (16) of the microscope body (15) and/or the stand (2) has a light source (17) provided therein whose light (17a, 17b, 17c) can pass outwardly through passage openings (18, 24, 27).

Abstract: It is possible to check for observation success or failure and the observation history without waiting for observation to be completely finished, thus saving time and energy required for observation, and avoiding lost opportunities for observation of precious samples etc. Provided is a microscope apparatus including an image acquisition unit for acquiring a plurality of frame images while varying a plurality of parameters; an image saving unit for successively saving the frame images acquired by the image acquisition unit; a property-information saving unit for saving property information in which identifying information of the saved frame images is associated with the parameters; and a control unit for controlling these units, wherein the control unit saves updated property information in the property-information saving unit each time the frame image is saved in the image saving unit.

Abstract: A microscopic apparatus and an observing method can obtain information of a super-resolved image of an object under observation with a high SN ratio. Therefore, the microscopic apparatus including an illuminating optical system that illuminates a specimen plane of a sample with line-shaped illuminating light, a modulating unit that spatially modulates the illuminating light in a lengthwise direction, an image-forming optical system that forms an image of light from the specimen plane illuminated with the spatially modulated illuminating light, and a detector that detects light from the specimen plane. Accordingly, a confocal effect is obtained with respect to an unstructuring direction of an illuminated area. The essential contrast of the structured illumination of the illuminated area is enhanced by the confocal effect, and thus the modulated image of the illuminated area is detected with a high SN ratio.

Abstract: A device for histological research includes at least one container with shelves for slide trays connected to a flexible drive enabling reciprocating vertical motion of the container. The drive comprises a circular drive cable, a motor and at least one pulley. The device also includes a horizontal pusher provided on the working end with a tool for gripping the slide tray and moving it into the working zone outside the container and then back into the container, as well as a slide gripper capable of moving horizontally over the working zone in the direction perpendicular to that of the horizontal pusher.

Abstract: A light field microscope incorporating a lenslet array at or near the rear aperture of the objective lens. The microscope objective lens may be supplemented with an array of low power lenslets which may be located at or near the rear aperture of the objective lens, and which slightly modify the objective lens. The result is a new type of objective lens, or an addition to existing objective lenses. The lenslet array may include, for example, 9 to 100 lenslets (small, low-power lenses with long focal lengths) that generate a corresponding number of real images. Each lenslet creates a real image on the image plane, and each image corresponds to a different viewpoint or direction of the specimen. Angular information is recorded in relations or differences among the captured real images. To retrieve this angular information, one or more of various dense correspondence techniques may be used.

Abstract: A method and an apparatus are disclosed for scatterfield microscopical measurement. The method integrates a scatterometer and a bright-field microscope for enabling the measurement precision to be better than the optical diffraction limit. With the aforesaid method and apparatus, a detection beam is generated by performing a process on a uniform light using an LCoS (liquid crystal on silicon) or a DMD (digital micro-mirror device) which is to directed to image on the back focal plane of an object to be measured, and then scattered beams resulting from the detection beam on the object's surface are focused on a plane to form an optical signal which is to be detected by an array-type detection device. The detection beam can be oriented by the modulation device to illuminate on the object at a number of different angles, by which zero order or higher order diffraction intensities at different positions of the plane at different incident angles can be collected.

Abstract: An immersion microscope objective formed of thirteen or fewer lens elements includes, in order from the object side, first and second lens groups of positive refractive power, a third lens group, a fourth lens group having negative refractive power with its image-side surface being concave, and a fifth lens group having positive refractive power with its object-side surface being concave. The first lens group includes, in order from the object side, a lens component that consists of a lens element of positive refractive power (when computed as being in air) and a meniscus lens element having its concave surface on the object side. Various conditions are satisfied to ensure that images of fluorescence, obtained when the immersion microscope objective is used in a laser scanning microscope that employs multiphoton excitation to observe a specimen, are bright and of high resolution. Various laser scanning microscopes are also disclosed.

Abstract: It is an object to perform high-precision observation by compensating group-velocity-delay dispersion and angular dispersion with a simple structure. The invention provides a laser microscope 1 including a light source; an acousto-optic deflector 7 that deflects ultrashort-pulse laser light L emitted from the light source; an angular-dispersion element 8, disposed in front of or after the acousto-optic deflector 7, that applies angular dispersion in a direction opposite to the acousto-optic deflector 7; and a group-velocity-delay dispersion-amount adjusting unit 10 that adjusts the amount of dispersion compensation by moving the angular-dispersion element 8 so as to vary the optical path length at each wavelength between the angular-dispersion element 7 and the acousto-optic deflector 8.

Abstract: A microscope apparatus efficiently supplies and collects a liquid for observation by local liquid immersion. The apparatus includes an objective of a liquid immersion system, a discharging member for discharging the liquid between a front edge of the objective and a substrate, and a sucking member for sucking the liquid. Inclined faces are provided respectively in two positions adjacent to the front edge in the periphery of the objective, and protruded portions are provided in positions adjacent to the inclined faces. An aperture portion bounded by the protruded portions and the substrate is formed at the side face. The discharging member includes a tubular member provided on the inclined face for discharging the liquid. The sucking member includes another tubular member for sucking the liquid while taking in air via the aperture portion.

Abstract: A lighting optical apparatus using a deep ultraviolet light source that are easy to adjust due to a configuration with fewer components, has high illuminant and illuminant uniformity on an irradiated surface are provided. The apparatus has a deep ultraviolet light source from which deep ultraviolet rays are emitted, a first double-sided cylindrical lens which has a cylindrical lens array on both sides with a configuration of cylinder axes intersecting at right angles, a second double-sided cylindrical lens which has a cylindrical lens array on both sides with a configuration of cylinder axes intersecting at right angles, and a condenser lens.

Abstract: Providing a microscope capable of movably adjusting an observation field of a sample without moving the sample. The microscope includes a first objective lens, a second objective lens, a mirror, an angular adjustment mechanism, and a shift mechanism. The first objective lens is disposed to the sample side. The second objective lens forms an intermediate image of the sample together with the first objective lens. The mirror is disposed with a tilt on an optical path between the first objective lens and the second objective lens. The angular adjustment mechanism rotatably adjust the mirror in the tilt direction. The shift mechanism makes a shift adjustment of the second objective lens in an axial direction of a rotation axis of the mirror. With the configuration, the observation field can be moved two-dimensionally by the angular adjustment mechanism.

Abstract: An examination apparatus that observes a specimen in a stationary state while suppressing the blurring caused by a control delay. The apparatus includes a first optical system and a second optical system for imaging light produced in a specimen, a first image-acquisition unit with a plurality of first image-acquisition devices for detecting an image formed by the first optical system, a second image-acquisition unit with a second image-acquisition device for acquiring an image formed by the second optical system, and a driving unit that causes the images to be formed at the same position in the second image-acquisition unit. The value obtained by dividing the pixel size Y of the first image-acquisition devices by the magnification X of the first optical system is smaller than the value obtained by dividing the pixel size Y? of the second image-acquisition device by the magnification X? of the second optical system.

Abstract: An imaging system, methodology, and various applications are provided to facilitate optical imaging performance. The system contains a sensor having one or more receptors and an image transfer medium to scale the sensor and receptors in accordance with resolvable characteristics of the medium, and as defined with certain ratios. Also provided are digital images that contain a plurality of image pixels, each image pixel containing information from about one sensor pixel, each sensor pixel containing substantially all information from about one associated diffraction limited spot in an object plane. Methods of making digital images are provided.

Abstract: A method and objective apparatus are provided for implementing an enhanced phase contrast microscope. A focusing vortex lens, defined by a diffractive spiral zone plate (SZP) lens, is used for the objective for the phase contrast microscope. The SZP lens focuses and imparts a helical phase to incident illumination to image the specimen with spiral phase contrast. The spiral phase contrast microscope is sensitive to phase gradients in all sample axes. Replacing the objective of a microscope with the diffractive SZP lens of the invention immediately provides existing instruments with spiral phase contrast capability.

Abstract: To provide a height detection apparatus capable of detecting height information of a subject surface even in a narrow area, the height detection apparatus includes: an illumination optical system for illuminating the subject surface with a light flux emitted from a light source unit; an imaging optical system for causing the light flux reflected on the subject surface to form an image as a line image on an imaging surface; and a light detection unit disposed on the imaging surface. A light detection surface of the light detection unit is positioned in a plane rotated about a rotational axis, the rotational axis being an axis which is perpendicular to an optical axis of an optical element positioned optically closest to the light detection unit among optical elements constituting the imaging optical system, and is perpendicular to the line image to be formed by the imaging optical system.

Abstract: There is provided a retention mechanism for an immersion medium, for use in a device which observes/measures a sample by use of an immersion objective lens, includes a member which retains the immersion medium near a tip portion of the objective lens, wherein the member is configured to include at least first and second materials.

Abstract: A tube unit for microscopes which has a tube lens, including two components with an intermediate, large air separation and an overall positive refractive power. The air separation is at least half the size of the focal length f of the tube lens. A roof edge mirror or another suitable deflection element is arranged between the two components of the tube lens. The roof edge mirror includes two mirrors which can be tilted with respect to one another, and which is able to be tilted around its roof edge. The tilting movement or the tilting angle of the tiltable mirror or deflection element corresponds to half the tilt or half the tilting angle of the tube or eyepiece viewing system.

Abstract: A clear image having suppressed blurring due to pulsing is obtained by in-vivo examination of biological tissue or various internal organs of mammals, including small laboratory animals.

Abstract: An observation apparatus for observing an observation object mounted at an observation position on a stage through an immersion objective lens placed below the observation object. The observation apparatus has a liquid pouring device, constructed to be relatively movable with respect to the immersion objective lens, pouring a liquid on a top lens surface of the immersion objective lens from the upper side of the immersion objective lens at a distance from the observation position on the stage; and a position control device automatically adjusting relative positions of a liquid pouring position of the liquid pouring device and the immersion objective lens and automatically adjusting relative positions of the immersion objective lens on which the liquid is poured by the liquid pouring device and a desired observation position on the stage.

Abstract: A method and system for performing three-dimensional holographic microscopy of an optically trapped structure. The method and system use an inverted optical microscope, a laser source which generates a trapping laser beam wherein the laser beam is focused by an objective lens into a plurality of optical traps. The method and system also use a collimated laser at an imaging wavelength to illuminate the structure created by the optical traps. Imaging light scattered by the optically tapped structure forms holograms that are imaged by a video camera and analyzed by optical formalisms to determine light field to reconstruct 3-D images for analysis and evaluation.

Abstract: An apparatus adapted for con focal imaging of a non-flat specimen comprising a coherent light source for producing a light beam, imaging optics adapted to focus the light beam into at least one spot on a surface of a specimen, and a detector adapted to receive and detect light reflected from the specimen surface. The imaging optics comprise at last one optical component located so that the light reflected from the specimen surface passes therethrough on its way to the detector. The optical component is movable so as to move the at least one spot, within a range of movement, to a number of distinct locations in a plane perpendicular to the apparatus' optical axis, within the detector's integration time.

Abstract: A changing device for receiving optical elements, particularly for microscopes, has a stand comprising a side wall, front wall and rear wall. This changing device is arranged at a holder in the telecentric part of the microscope beam path and is outfitted with a quantity of optical components influencing the beam path. The arrangement is mounted so as to be rotatable around an axis of rotation which is situated in the holder and which intersects the optical axis of the microscope beam path.

Abstract: A compact, portable microscope for use in the field is provided with: an optical path extending within the microscope casing between a viewing port and an eyepiece element, which path is folded by a plurality of reflectors; and a cam and follower microscope focusing mechanism. The cam and follower microscope focusing mechanism is configured such that an objective lens element is moveable relative to a substantially planar viewing stage comprising the viewing port. A first portion of the folded optical path between the viewing port and a first reflector extends in a first plane substantially perpendicularly to a second plane in which the remainder of the optical path extends.

Abstract: A scanning microscope includes an acousto-optic scanner that produces a scanned beam. A beam separator based on total internal reflection or angle tuning of a dielectric filter separates an unscanned portion of an excitation light flux from a scanned portion. The scanned beam is directed to a specimen, and optical radiation generated in response to the scanned beam is directed to a detector that produces a detected signal that can be used to determine an image. The scanned beam can be directed to the specimen without formation of any intermediate focusing.

Abstract: An optical system for viewing an object has a plurality of lenses and a main optical axis coincident with the centers of the lenses. The optical system further comprises a low magnification optical subsystem that is operative to view the object at a first magnification and a high magnification optical subsystem that is operative to view the object at a second magnification that is higher than the first magnification. The high magnification optical subsystem has a high magnification optical axis along which light rays that are received from the main optical axis are transmitted. A movable element is locatable on the high magnification optical axis and is movable in directions transverse to the axis for receiving and transmitting light rays.

Abstract: Embodiments of the present invention can resolve molecules beyond the optical diffraction limit in three dimensions. A double-helix point spread function can be used to in conjunction with a microscope to provide dual-lobed images of a molecule. Based on the rotation of the dual-lobed image, the axial position of the molecule can be estimated or determined. In some embodiments, the angular rotation of the dual-lobed imaged can be determined using a centroid fit calculation or by finding the midpoints of the centers of the two lobes. Regardless of the technique, the correspondence between the rotation and axial position can be utilized. A double-helix point spread function can also be used to determine the lateral positions of molecules and hence their three-dimensional location.