Abstract: A signal processor includes a frequency generator that employs a direct digital synthesizer (DDS) to generate a first local oscillator (LO) signal with a variable first LO frequency. The signal processor also includes an oscillator generating a second LO signal having a second LO frequency. The DDS employs programmable frequency control word and a sampling clock signal having a variable sampling clock frequency that is derived from the second LO frequency, to generate a DDS output signal from which the first LO signal is produced. The variable sampling clock frequency and the programmable frequency control word are selected to avoid crossing spurs in the frequency spectrum of the DDS output signal.

Abstract: A constant switching current flip-flop includes a latch circuit that provides latch outputs of the flip-flop, whereby the latch outputs are reset to zero at the beginning of each clock cycle to eliminate pattern dependent switching currents. The latch circuit is reset responsive to control signals provided without significant delay.

Abstract: A sample detection apparatus (200) for detecting a fluidic sample flowing through a first flow cell (202) and flowing through a second flow cell (204) of a sample separation system (10), wherein the first flow cell (202) has a first path length (D) and the second flow cell (204) has a second path length (d) being smaller than the first path length (D), wherein the sample detection apparatus (200) comprises a data determining unit (206) configured for determining first data indicative of a first relation between a detection signal intensity and a concentration of the fluidic sample in the first flow cell (202), and configured for determining second data indicative of a second relation between a detection signal intensity and the concentration of the fluidic sample in the second flow cell (204), and a data combining unit (208) configured for combining the first data and the second data in accordance with a continuous weighting function to thereby derive a weighted relation between detection signal intensity and

Abstract: The present invention provides a simple and rapid method for preparing purified transposase complexes that are highly suited for fragmenting DNA. The method includes forming transposase complexes with oligonucleotide adapters in cell lysate, then purifying the complexes from the other substance in the cell lysate. Purification is accomplished using a specific binding pair, in which one member of the pair is bound to an oligonucleotide adapter of the complex and the other member of the pair is bound to a solid substrate. The bound complexes can be immediately used in DNA fragmentation reactions to produce solid substrate-bound DNA fragments, which can be used for any number of purposes, including as templates for amplification and sequencing.

Abstract: A device and a method of forming the same are disclosed. The device comprises a substrate and a thin film. The substrate is characterized by a first coefficient of thermal expansion. The thin film is attached to a surface of the substrate, and is characterized by a second coefficient of thermal expansion. The thin film includes first and second layers in states of compression, and a third layer in a state of tension, the third layer being positioned between the first and second layers. The thin film is in a net state of tension within a temperature range.

Abstract: Methods and systems for allelic detection and allele-specific copy number are provided herein. The described methods use identification of single nucleotide polymorphism using restriction enzymes and CGH analysis. Microarrays comprising probes designed by the described methods are provided. Also included are methods for identifying SNP sites and copy number in samples obtained from patient populations.

Abstract: Micelle-templated superficially porous particles having a solid core and an outer porous shell with ordered pore structures and a narrow particle size distribution, such as about ±5% (one sigma), and a high specific surface area of about 5 to about 1000 m2/g.

Abstract: The present invention provides compositions and improved methods for multi-site directed mutagenesis and DNA shuffling. The present compositions and methods provide increased mutation frequency and increased number of transformants which allow one to sequence only a few clones in order to identify the correct mutants and to obtain the desired mutant by screening large number of transformants in a short time. Moreover, the inclusion of FEN-1, PEF and optimized buffer and cycling conditions provided in the present invention should also facilitate random mutagenized library construction and the mutagenesis of large or difficult templates.

Abstract: The present invention provides a method for the isolation of biological molecules by the adsorption of the molecules onto a mineral substrate in the presence of an appropriate mixture of salts and sufolane. Preferably, the biological molecules are nucleic acids. Compositions and kits for performing the process according to the invention are also provided.

Abstract: A phase digitizing system includes an analog-to-digital converter (ADC), multiple phase accumulators and a processing device. The ADC generates sample segments of digital signal waveform samples based on an analog composite input signal received in a measurement channel, the composite input signal includes a first signal having a first frequency F1 and a second signal imported from a reference channel having a second frequency F2. The processing device is coupled to the phase accumulators, and digitally processes each sample segment with outputs of the phase accumulators, and continually generates digital phase data The processing device further provides increment values to each of the phase accumulators based on the digital phase data, causing an output of a first phase accumulator to represent an instantaneous phase of the first signal, and an output of a second phase accumulator to represent an instantaneous phase of the second signal.

Abstract: The invention provides novel ion-exchange media and related methods for their preparation and use. Ion-exchange stationary phases according to the invention are suitable for chromatographic separation of a variety of biomolecules. Distinguishing characteristics of ion-exchange media according to this invention includes, for example, their ability to separate variants of monoclonal antibodies via cation-exchange liquid chromatography using porous substrates with particle sizes <5 ?m. The ion-exchange stationary media include a hydrolytically stable layer, which inhibits surface degradation of the particles in 100% aqueous media. Another unique feature is low molecular weight building blocks used to functionalize the particles with ion-exchange groups.

Abstract: Ion guides for use in mass spectrometry (MS) systems are described. The ion guides are configured to provide a reflective electrodynamic field and a direct current (DC or static) electric field to provide ion beams that are more spatially confined with a comparatively large mass range. Some ion guides are provided between the ion source and the first stage vacuum chamber of the MS system.

Abstract: A method of generating a trend graph comprises storing a stream of data elements in a buffer having a storage capacity corresponding to a first number of data elements, compressing the stored data elements to fit within a display having a size corresponding to a second number of data elements smaller than the first number of data elements, detecting an event, and as a consequence detecting the event, compressing the stored data elements to occupy a reduced portion of the buffer.

Abstract: The area density of broadcast stations having respective service areas and broadcasting on the same channel is increased without exceeding a specified level of co-channel interference within the service areas by a method in which the field strength of the signal transmitted by the first broadcast station is monitored, and a field strength determining property of the first broadcast station is controlled in response to the monitoring.

Abstract: One embodiment of the invention provides a sample injection system including a vacuum source, a conduit in communication with the vacuum source, a fluid sensor configured to detect the presence of the fluid in the conduit, a sample loop in communication with the conduit; and a sipper in communication with the sample loop.

Abstract: A disruptor apparatus comprises a nozzle comprising: a converging section; a diverging section; and a throat between the converging section and the diverging section. The disruptor apparatus also comprises a holder configured to receive a fluid conduit, which comprises an outlet located in the converging section; and a channel disposed about the holder and configured to guide a gas past the outlet of the fluid conduit, through the converging section, through the throat and into the diverging section where the gas travels at supersonic speed and establishes a standing shock wave in the diverging section. A mass spectrometer and a method are also described.

Abstract: A method of operating a superconducting switch system to protect against quenching in a superconducting magnet circuit is provided. The system comprises a magnet circuit, a main superconducting switch and an auxiliary superconducting switch connected in parallel thereto by low resistance connecting parts. In an initial magnet energisation procedure, after establishing superconducting flow in the circuit, the main and auxiliary switches are closed to cause current to persist in the circuit without further power input. If the main switch quenches, the current will transfer to the auxiliary switch through the resistive connecting parts. Upon restoring the main switch to the superconducting state, the voltage drop through the resistive connecting parts will drive the current back through the main switch. A preconditioning procedure is carried out as part of the magnet energisation procedure to ensure that opening the main switch will establish superconducting current through the auxiliary switch.

Abstract: A microwave imaging system uses microwave radiation provided by a microwave source to image targets. The system includes an array of antenna elements that are capable of being programmed with a respective direction coefficient to direct the microwave illumination from the microwave source toward a position on the target. The antenna elements are further capable of being programmed to receive reflected microwave illumination reflected from the position on the target. A processor is operable to measure an intensity of the reflected microwave illumination to determine a value of a pixel within an image of the target. Multiple beams can be directed towards the target to obtain corresponding pixel values for use by the processor in constructing the image.

Abstract: An amplifier system and method that eliminates memory effects due to amplifier sharing. The amplifier has a plurality of input stages. An input to be amplified is applied to one of the input stages of the amplifier, while the other input stages are turned off and reset. The inputs of the unused input stages are thus reset and equalized while the other input stage is turned on to receive the input to be amplified. An explicit reset phase is not needed.

Abstract: Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture.