Abstract: Disclosed are constructs, systems, and methodologies using prime editing (PE), twin prime editing (twinPE), or multi-flap prime editing to carry out site-specific and large-scale genetic modification, such as, but not limited to, insertions, deletions, inversions, replacements, and chromosomal translocations of whole or partial genes (e.g., whole gene, gene exons and/or introns, and gene regulatory regions). In certain embodiments, the disclosure provides constructs, systems, and methods using prime editing (PE), e.g., single flap or “classical” PE or twinPE or multi-flap PE, to install one or more target sites for site specific recombination in a target genomic locus (e.g., a specific gene, exon, intron, or regulatory sequence), which may then be acted on by one or more site-specific recombinases to effectuate a large-scale genetic modification, such as an insertions, deletions, inversions, replacements, and chromosomal translocations.

Abstract: The present disclosure provides compositions and methods for the targeted modification of RNA molecules by RNA prime editing. The compositions and methods may be conducted invitro or in vivo within cells (e.g., human cells) for the therapeutic correction of disease-causing mutations and/or installation of motifs or mutations in RNA molecules of interest as a tool for scientific research. The disclosure provides compositions and methods for conducting RNA prime editing of a target RNA molecule (e.g., an RNA transcript) that enables the incorporation of one or more nucleotide changes and/or targeted mutagenesis of a target RNA molecule. The nucleotide change can include a single-nucleotide change, an insertion of one or more nucleotides, or a deletion of one or more nucleotides.

Abstract: The present disclosure provides new prime editor guide RNAs for prime editing, constructs for prime editing, and methods for using same. In addition, the present disclosure provides compositions and methods for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis (e.g., insertion or deletion). The nucleotide change can include a single-nucleotide change (e.g., any transition or any transversion), an insertion of one or more nucleotides, or a deletion of one or more nucleotides. More in particular, the disclosure provides fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a prime editor RNA (PEgRNA).

Abstract: The disclosure provides compounds that inhibit the invasion of host cells by intracellular pathogens. The disclosure also relates to use of such compounds in methods of treating and preventing periodontitis or a periodontitis-related condition or symptom.

Abstract: Disclosed is a composition suitable for use as an anode in a lithium ion rechargeable batter. The composition includes milled silicon powder particles and superconducting carbon particles. A carbon coat covers the surface of each milled silicon particle and encapsulates a plurality of superconducting carbon particles. Also, disclosed is a method of preparing the disclosed composition.

Abstract: A sensing probe may be formed of a diamond material comprising one or more spin defects that are configured to emit fluorescent light and are located no more than 50 nm from a sensing surface of the sensing probe. The sensing probe may include an optical outcoupling structure formed by the diamond material and configured to optically guide the fluorescent light toward an output end of the optical outcoupling structure. An optical detector may detect the fluorescent light that is emitted from the spin defects and that exits through the output end of the optical outcoupling structure after being optically guided therethrough. A mounting system may hold the sensing probe and control a distance between the sensing surface of the sensing probe and a surface of a sample while permitting relative motion between the sensing surface and the sample surface.

Abstract: An optical system, comprising: (i) multiple input optical fibers; (ii) an optical mode multiplexer/demultiplexer coupled to said input optical fibers with, said optical mode multiplexer/demultiplexer comprising a plurality of metamaterial structures having length and forming at least one stage of metamaterials, the at least one stage of metamaterials is being situated on a surface of the optical mode multiplexer/demultiplexer facing the input optical fibers, and the at least one stage of metamaterials is oriented at angles between 60 and 120 degrees relative to the axis of the input fibers; and the metasurfaces are structured to receive a first optical signal having a first mode from at least one of said multiple input optical fibers and convert the first mode to a different mode.

Abstract: The disclosure features compositions and methods that are useful for determining the fraction of tumor-derived DNA (tumor fraction; TF) in cell free DNA (cfDNA). The methods involve calculating the fraction of tumor-derived DNA in the cfDNA using a combination of copy number alteration data and fragment length distribution data.

Abstract: This invention relates to methods and compositions for assessing risk by measuring total and specific cell-free nucleic acids (such as DNA) in a subject. The methods and compositions provided herein can be used to determine risk of a condition, such as transplant rejection.

Abstract: The technology described herein is directed to targeting molecules that bind to one or more transmembrane molecules expressed in tumor vascular endothelial cells and that are capable of targeting an agent that induces cell death, for example, immunogenic or non-immunogenic cancer cell death. In another aspect, described herein are methods of treating a subject having cancer comprising administering said targeting molecules and agents, and, in certain embodiments, enhancing the immunogenic response to the cancer, for example, by enhancing intratumoral infiltration of T cells.

Abstract: The technology described herein is directed to antibody or antigen binding fragments thereof, e.g., nanobody, to PMEPA-1 overexpressed in tumor vascular endothelial cells. The antibody or antigen binding fragments to PMEPA-1 can be used to target an agent, for example, that induces cell death, for example, immunogenic or non-immunogenic cancer cell death, and in methods of treating cancer.

Abstract: Systems and methods relate to selectively arranging a plurality of qubits into a spatial structure to encode a quantum computing problem. Exemplary arrangement techniques can be applied to encode various quantum computing problems. The plurality of qubits can be driven according to various driving techniques into a final state. The final state can be measured to identify an exact or approximate solution to the quantum computing problem.

Abstract: Methods for treating and for preventing filovirus infections are disclosed, as well as compositions therefor.

Abstract: The invention is directed generally to oligonucleotide compositions for the treatment of DNA repeat expansion diseases. The invention also relates to oligonucleotides directed to subunits of the DNA mismatch repair system.

Abstract: The present invention has to do with a method and system for peritoneal dialysis catheter weighted anchors. The peritoneal dialysis catheter weighted anchors can be inserted and retrieved using standard techniques and available insertion/retrieval systems. The peritoneal dialysis catheter weighted anchors can be attached and secured to all types of PD catheters as an ancillary anchor device.

Abstract: Compositions and methods for making a plurality of probes for analyzing a plurality of nucleic acid samples are provided. Compositions and methods for analyzing a plurality of nucleic acid samples to obtain sequence information in each nucleic acid sample are also provided.

Abstract: Embodiments of the disclosure include a tissue and/or access device that allows for adjustability of placement of a needle for accessing a blood vessel of an individual in need thereof, particularly when used with an imaging device. The adjustability originates from both angle of the needle for insertion into the body of the individual and longitudinal placement of the needle on the body of the individual. In specific embodiments, a needle guide through which the needle traverses is detachable.

Abstract: The present application provides a VR-based standardized training system for multi-person online cooperated first-aid nursing of trauma, which is used for providing medical staff with an efficient and convenient-to-update and maintain standardized training environment for multi-person online cooperated first-aid nursing of trauma by introducing the VR technology, so as to significantly improve the training effect of the first aid of trauma of the medical staff.

Abstract: The specification provides programmable base editors that are capable of introducing a nucleotide change and/or which could alter or modify the nucleotide sequence at a target site in a double-stranded nucleotide sequence, such as, a chromosome, genome, or a mitochondrial DNA (mtDNA), with high specificity and efficiency. Moreover, the disclosure provides fusion proteins and compositions comprising a programmable DNA binding protein (e.g., a mitoTALE, a mitoZFP, or a CRISPR/Cas9) and evolved double-stranded DNA deaminase domains that is capable of being delivered to a cell nucleus and/or a mitochondria and carrying out precise installation of nucleotide changes in the target a double-stranded nucleotide sequence, such as, a chromosome, genome, or mtDNA. The fusion proteins and compositions are not limited for use with mtDNA, but may be used for base editing of any double-stranded target DNA.

Abstract: A membrane filtration cell is provided which includes a fluid passageway and a filtration membrane positioned within the passageway, the filtration membrane dividing the fluid passageway into two chambers, a retentate chamber and a permeate chamber. A first electrode is positioned in the retentate chamber and a second electrode is positioned in the permeate chamber, where the first electrode and the second electrode are configured to apply an electric field across the filtration membrane. The membrane filtration cell also includes an acoustic device configured to apply an acoustic field across the retentate chamber, where the synergistic combination of the electric field and the acoustic field prevents fouling on the filtration membrane.