Abstract: The present invention is directed to isolated nucleic acid molecules and polypeptides of thraustochytrid polyunsaturated fatty acid (PUFA) synthases involved in the production of PUFAs, including PUFAs enriched in docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or a combination thereof. The present invention is directed to vectors and host cells comprising the nucleic acid molecules, polypeptides encoded by the nucleic acid molecules, compositions comprising the nucleic acid molecules or polypeptides, and methods of making and uses thereof.

Abstract: The invention relates to a method for producing linear DNA molecules with at least two or more repeating units of a target DNA sequence for use as DNA substrate in single-molecule assays, wherein a target DNA is subjected to a ligation reaction and the ligation reaction is stopped when the reaction reaches the end of the linear phase to block circularization of the DNA molecules. Compositions comprising such linear DNA molecules and use of such compositions in single-molecule assays are also provided.

Abstract: The present invention relates to a method of reducing methane emissions in ruminants, said method encompassing treating pre-/young ruminants with 3-nitrooxypropanol.

Abstract: The present invention relates to a variant polypeptide having a lipase activity, wherein the polypeptide may be a polypeptide that has an amino acid sequence which has at least 70% identity to a mature amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 6, wherein the polypeptide comprises an amino acid substitution at position 295 and at least one further amino acid substitution at position 113, 121, 179 and/or 284, wherein the positions are defined with reference to SEQ ID NO: 1 or SEQ ID NO: 6. The invention further relates to a process for preparing a dough wherein a polypeptide as disclosed herein is used and baked product prepared from the dough.

Abstract: A method of detecting a biomolecular process is provided. The method comprises: providing a DNA strand (11) comprising one or more luminophores (3) attached to the strand (11) at one or more predetermined positions along the strand; attaching handles (7A, 7B) to the DNA strand; and trapping and/or manipulating the strand (11) by manipulating the handles (7A, 7B) in a trapping setup. The method also comprises determining a position of at least one of the one or more luminophores (3) with respect to at least part of the trapping setup and determining on the basis of the one or more luminophores (3) one or more of: a focus position of at least part of an optical detector and/or an illumination system; a focus position of at least part of an optical manipulator; a position of at least part of the strand (11); a position of a portion of the strand (11) relative to one or more other portions of the strand (11); a reaction or binding position on the strand (11) of a reagent interacting with the strand (11).

Abstract: The present disclosure relates to the production of sialylated Human Milk Oligosaccharides (HMOs), in particular to the biosynthetic production of 3?-sialyllactose (3?SL), and to genetically engineered cells and methods suitable for said production.

Abstract: A waterproof seal for electrical assemblies wherein at least two pins extend through the electrical conductor such that the at least two pins extending from a distal end of the electrical conductor and are adapted to interact with openings on a connecter that is detachably connectable with the distal end of the electrical connector, and the at least two pins extend from a proximal end of the electrical connector such that the at least two pins extending from the proximal end are adapted to be interact with openings in a socket formed in the surface of housing where the electrical connector forms a water tight barrier preventing water from entering the housing through the electrical connector or around the electrical connector.

Abstract: Disclosed herein is a method of making a composite fabric for use in or as a medical implant component, the method comprising steps of providing a textile comprising at least one strand having titer of 2-250 dtex and comprising fibers made from a biocompatible and biostable synthetic polymer; determining locations on the textile where a cut is to be made for an intended use of the textile; optionally pretreating the textile at least at the determined locations on at least one side of the textile with a high-energy source to activate the surface; solution coating the textile at least at a determined location with a coating composition comprising a biocompatible and biostable polyurethane elastomer and a solvent for the polyurethane; removing the solvent from the coated textile; and laser cutting the coated textile as obtained at least a one coated location with an ultra-short pulse laser; to result in a composite biotextile wherein polyurethane is present in an amount of 2.

Abstract: Disclosed herein is a medical implant component comprising a composite biotextile, which biotextile comprises i) a polyolefin fibrous construct comprising at least one strand with titer of 2-250 dtex, tensile strength of at least 10 cN/dtex and comprising high molar mass polyolefin fibers and ii) a coating comprising a biocompatible and biostable polyurethane elastomer comprising a polysiloxane segment and/or having one or more hydrophobic endgroups, wherein the polyurethane coating is present on at least part of the surface of the biotextile and in an amount of 2.5-90 mass % based on composite biotextile. Such composite biotextile, like a partly coated woven fabric, shows an advantageous combination of good biocompatibility, especially hemocompatibility, high strength and pliability, and laser cuttability; allowing to make pieces of fabric having well-defined regular edges that have high suture retention strength. The invention also provides a method of making said composite biotextile.

Abstract: Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition, treating the lysed cell composition to form an oil-containing emulsion and then recovering the oil from the oil-containing emulsion. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

Abstract: The present invention relates to a process for reducing haze in a beverage comprising adding a protein arginine deiminase and a proline-specific endoprotease during preparation of the beverage. The invention further relates to a wort or beer comprising a protein arginine deiminase and a proline-specific endoprotease.

Abstract: The present invention relates to a method for reducing fructan in a fructan-containing food product, including adding an invertase belonging to enzyme classification EC 3.2.1.26 to the food product, and incubating the food product with the invertase, wherein fructan in the fructan-containing food product is hydrolysed. The invention further relates to the use of an invertase belonging to enzyme classification EC. 3.2.1.26 for the preparation of a medicament or a dietary supplement for the treatment of a person suffering from irritable bowel syndrome. Invertase (EC 3.2.1.26) may optionally by further combined with inulinase (EC3.2.1.7) and/or beta-fructosidase (EC 3.2.1.80).

Abstract: The present invention relates to a new process of production of menaquinone 4, which is also known as vitamin K2.

Abstract: The present invention relates to a new process for the production of retinal or hydrogenated form of retinal.

Abstract: The invention relates to an extrusion apparatus for the continuous production of solid food supplements, feed additives or pharmaceutical preparations, which comprise nutritional or pharmaceutical actives homogeneously dispersed in a carrier material matrix. The invention also relates to a method for the production of such preparations by extrusion.

Abstract: A waterproof seal for electrical assemblies wherein an outer sheath of an electrical cable is removed to expose the wires and the insulation is removed from the wires to form a window of exposed electrical conductor, the electrical conductors being maintain apart from each other and contained in a cover that may comprise a connector, an epoxy applied to the electrical conductors to fill in all the space in and around the electrical conductors and the cover to form a waterproof seal that prevents water from wicking past the waterproof seal via an interior of the electrical cable.

Abstract: The present invention relates to animal feed compositions comprising at least one probiotic and polypeptides having muramidase activity.

Abstract: The disclosure relates to a method of making a bioceramic coating on a fibrous article for use in a medical implant, comprising steps of providing an article comprising fibers made from a biocompatible, non-biodegradable polymer; coating at least the fibers that will be in contact with bone upon use as an implant with a solution of a coating polymer to result in coated fibers having a coating polymer layer; treating the coated fibers with a dispersion of bioactive ceramic particles 0.01-10 ?m in a treating solvent comprising a solvent for the coating polymer in at least one step; and substantially removing the treating solvent; to result in the particles being partly embedded in the coating polymer layer of the coated fibers.