Abstract: A process is provided for producing an ordered series of cloned, circular, DNA molecules containing shortened target DNA segments derived from a long target DNA segment, which are suitable for use in determining the nucleotide sequence of the long target DNA segment, or for targeting specific regions within the target DNA segment. The process includes producing, by molecular cloning, a plurality of double-stranded recombinant DNA molecules each containing: (i) vector DNA; (ii) a sequencing primer binding site; and, (iii) a DNA region having unique endonuclease sites and a long target DNA segment. The sequencing primer binding site is spaced from the long target DNA segment by at least a portion of said DNA region having the unique endonuclease sites. The plurality of double-stranded circular recombinant DNA molecules are cleared using two restriction endonucleases.

Abstract: An electronic fuel control device is used to activate a fuel system carried on board a motor vehicle independently of a computer system carried on board the vehicle. The fuel control device embodies an electrical circuit to create an output of a series of pulses of electrical energy. Electrical power is carried to the electrical circuit by electrical supply conducting apparatus. The pulses of electrical energy are transported from the electrical circuit to the fuel injecting system by electrical pulse conducting apparatus connected to the fuel injecting system.

Abstract: Hybridoma cell lines that produce monoclonal antibodies that differentially recognize glycolipids with mono-, di-, and trifucosylated type 2 chain structures are disclosed. The monoclonal antibodies can be used to detect specific types of tumor cells that are characterized by enrichment in mono-, di-, or trifucosylated type 2 chain structure. As such, the antibodies produced by the hybridoma cell lines are useful for diagnosis and treatment of human cancer. Also disclosed is an improved method of raising hybridoma cell lines by selecting the hybridomas by positive reactivity with one or more fucosylated type 2 chain structures selected from the group consisting of III.sup.3 FucnLc.sub.4, V.sup.3 FucnLc.sub.6, III.sup.3 FucnLc.sub.6, III.sup.3 V.sup.3 Fuc.sub.2 nLc.sub.6, and III.sup.3 V.sup.3 VII.sup.3 Fuc.sub.n nLc.sub.8.

Abstract: DNA constructs consisting essentially of the promoter, gag, pol, and env sequences of a helper virus useful for making retrovirus packaging cell lines that do not yield helper virus and do not transfer the packaging function. Such DNA molecules are constructed by deleting from the genome of a replication-competent retrovirus all cis-acting elements except for the tRNA binding site. Specifically, deletion is made of the packaging signal, the site for initiation of second strand DNA synthesis, the site required for translation of reverse transcriptase during first strand DNA synthesis, and the provirus integration signal. DNA construct pPAM3 (ATCC No. 40234) is a representative embodiment. A cell line containing such an altered viral genome does not transmit this virus or transfer the packaging signal, but will transmit high titers of other viral RNAs containing the proper cis-acting elements, including retroviral vectors designed to carry foreign genes. Cell line PA317 (ATCC No.

Abstract: Disialosyl Le.sup.a (IV.sup.3 NeuAcIII.sup.6 NeuAcIII.sup.4 FucLc.sub.4), a novel human cancer-associated fucoganglioside that is highly immunogenic. Also, a hybridoma cell line (ATCC No. HB 8861)secreting a monoclonal IgG3 antibody (FH7) directed to disialosyl Le.sup.a. The disialosyl Le.sup.a antigen was detected in various cancer tissues and their cell lines but was absent in various normal tissues and blood cells. Sera of patients with various cancers, particularly early cases of colonic and gastric cancers, showed an elevated disialosyl Le.sup.a antigen level, which subsequently decreased after surgical tumor removal.

Abstract: A process is provided for producing an ordered series of cloned, circular, DNA molecules containing shortened target DNA segments derived from a long target DNA segment, which are suitable for use in determining the nucleotide sequence of the long target DNA segment, or for targeting specific regions within the target DNA segment. The process includes producing, by molecular cloning, a plurality of double-stranded recombinant DNA molecules each containing: (i) vector DNA; (ii) a sequencing primer binding site; and, (iii) a DNA region having unique endonuclease sites and a long target DNA segment. The sequencing primer binding site is spaced from the long target DNA segment by at least a portion of said DNA region having the unique endonuclease sites. The plurality of double-stranded circular recombinant DNA molecules are cleared using two restriction endonucleases.

Abstract: An improved traffic signal directional indicator device comprises a housing which may be mounted onto a conventional traffic signal and be illuminated to provide indicia pertaining to a motorist's particular direction of travel, i.e. North, Northeast, South, etc. The improved traffic signal directional indicator device may be rapidly retrofitted onto existing traffic signals and function without adversely affecting the normal operation of the traffic signal.

Abstract: A method of regulating oncogene-mediated cell transformation in a mammalian host. Oncogenes having glycosylated expression products are regulated by administering an effective amount of a processing glucosidase inhibitor: a glucosidase I inhibitor, e.g., castanospermine, N-methyl-1-deoxynojirimycin, 1-deoxynojirimycin, 5-amino-5-deoxy-D-glucopyranose; or a glucosidase II inhibitor, e.g., bromoconduritol. The glucosidase I inhibitors are preferred, particularly castanospermine (CA) and N-methyl-1-deoxynojirimycin (MdN). Oncogenes having glycosylated expression products that are ultimately expressed on the plasma membrane or secreted from transformed cells are particularly susceptible to regulation by these anti-cancer drugs. Also provided is a method of regulating the immune system of a mammalian host. Administration of an effective amount of a processing glucosidase inhibitor suppresses proliferation and differentiation of monocytic and myeloblastic cells.

Abstract: A buoyant support unit and platform employing the same which includes at least five substantially cylindrical floatable pipes having a diameter of less than about 5 meters which are arranged parallel to each other in spaced-apart relationship, wherein the edge to edge distance between adjacent pipes is from 0.25 to 2.0 times the diameter of the pipe.

Abstract: A ballpoint pen of conventional size and shape has a movable ball point that is pressed upwardly in the direction of the housing of the pen when a mark is made. Means is provided for normally biasing the ballpoint outwardly of the housing. However, when the ballpoint is pressed against a writing surface, the refill cartridge having the ball at the lower end thereof is moved upwardly to close a normally open switch which connects a solar cell array in circuit with an electronic counter that counts each time the switch is closed. Controlled by the counter is a liquid crystal display that visually indicates the total number of counts. A gravity switch is contained in the housing and is actuated to reset or clear the counter in preparation for counting a succeeding group of marks when the pen is inverted.

Abstract: Inclined chutes are filled with game balls of different colors or other visual characteristics. The underlying wall of each chute is formed of elastomeric material with openings therein of smaller diameters than the diameters of the balls so that the balls may roll down the chute and rest against each other and against a stop under the influence of gravity. Depressible plungers aligned with the openings may be selectively depressed to displace underlying balls through the openings from which they are guided to a display station.

Abstract: An electromagnetically driven vibratile piston underwater transducer element generates acoustic intensity levels in excess of 25 peak Watts per square inch of radiating surface in the low audible frequency region within the approximate range 500 to 2500 Hz. The design achieves an efficiency greater than 50% and a very low Q of less than 2. The electromagnetic design employs two separate independent air gaps and a unique mechanical coupling system that combines the electromechanical forces generated in each gap in the proper phase and transmits the combined oscillatory force to drive the vibratile piston.

Abstract: A prefabricated building panel comprising a plurality of longitudinally extending transversely spaced trusses, each truss comprising opposed channel members and undulating truss member joined to and extending between the channel members. One of the channel members of each truss is fastened to a sheet. A plurality of spring clips are fastened to the other of the channels at longitudinally spaced points. Each clip includes wings extending transversely of the channel. A second sheet is provided and has longitudinally extending grooves with the grooves having a configuration such that the spring clips extend into the grooves and the wings engage portions of the grooves to laterally restrain the sheet.

Abstract: A polygonal bulk container comprising a main body blank including a plurality of rectangular panels hingedly connected along hinge lines serialtim, a polygonal bottom panel having a majority of the side edges conforming to and abutting against the adjacent inner walls of said main body blank, a side edge glue flap hingedly connected to a margin of one of the rectangular panels, a bottom reinforcing tab with adhesive on the bottom surface thereof being foldable inwardly and glued to said polygonal bottom panel and functioning as a support therefor, and a polygonal auxiliary bottom panel in said container overlying said bottom reinforcing tab. The bottom tab and glue flaps together with adjacent extensions functioning to strengthen the bottom panel of the container against collapsing when transporting bulk food products.

Abstract: The power handling capacity of a high-power low-frequency underwater transducer including an inertial mass-loaded vibratile piston assembly is greatly increased by increasing the heat conduction from the inertial mass member by designing the vibrating structure to place a surface area portion of the inertial mass member in direct thermal contact with the water environment in which the transducer is submerged. In order to reduce the sound radiation from the exposed surface of the submerged inertial mass member and to obtain a broadband response with a Q equal to 1 or less, a novel rigid hollowed piston construction is described which greatly reduces the weight-to-stiffness ratio of the vibratile plate and achieves the desired low Q as well as a reduction of sound radiation from the exposed inertial mass surface by at least 20 dB below the radiation from the surface of the vibratile plate member.

Abstract: A ballpoint pen of conventional size and shape has a movable ball point that is pressed upwardly in the direction of the housing of the pen when a mark is made. Means is provided for normally biasing the ballpoint outwardly of the housing. However, when the ballpoint is pressed against a writing surface, the refill cartridge having the ball at the lower end thereof is moved upwardly to close a normally open switch which connects a solar cell array in circuit with an electronic counter that counts each time the switch is closed. Controlled by the counter is a liquid crystal display that visually indicates the total number of counts. A second switch is actuated by gravity to reset or clear the counter in preparation for counting a succeeding group of marks.

Abstract: An inspection system for automatically documenting and verifying the position and dimensions of holes in a flat sheet metal workpiece. The system includes a transparent, light diffusing inspection table upon which the workpiece is laid. A ball screw-driven yoke travels continuously along the inspection table in the Y direction and includes a bottom bar for positioning a source of illumination below the table and a top bar for positioning a plurality of optical sensor arrays above the table. The optical sensor arrays are responsive to the presence (or absence) of light energy which is diffused by the table. A detector is interconnected to the sensor arrays to detect light-to-dark and dark-to-light transitions which are representative of the edge of a hole. The transition data is read and stored in a microcomputer for subsequent display and/or print out.

Abstract: A rescue axe is used for forcibly entering a vehicle. The axe comprises a head portion located at one end of a handle portion. The head portion includes a piercing entry section, a cutting section, and an impacting section. The entry section includes a metal piercing member on one side of the head portion. The impacting section includes a striking edge on a side opposite the metal piercing member. The cutting section includes a blade cutting edge extending rearwardly from an open mouth located on a side of the head portion between the one side including the piercing member and the opposite side including the striking edge. The head portion includes a blade member located between the blade cutting edge and the striking edge. The handle portion extends rearwardly from a further side of the head portion opposite the open mouth side of the head portion.

Abstract: A spacer/tie rod to space and hold reusable sections of concrete molds is, according to the principal embodiment of this invention, constructed of two main elements and two auxiliary elements cut from the same small diameter steel rod stock. The main elements are identical, with each having an elongated central section and upturned indexing sections at each end, while the auxiliary elements are identical and straight. The latter are welded to the former at positions where the auxiliary elements are spaced apart by a distance equal to a wall thickness, and the distance between each auxiliary element and the main element indexing section nearest to said auxiliary element is equal to the thickness of the frame that stiffens a form section. The indexing sections of the main elements are tool bendable between form positioning and form holding positions.