Abstract: An artificial fishing lure. The lure has a bifurcated frame that presents upper and lower arm portions. An eye is presented from the juncture of the upper and lower arm portions. A hook means trails from the lower arm portion, and a spinner blade trails from the upper arm portion. A dive plate has a generally fan-shaped outline with a curvilinear rim that converges to an apex. The apex is connected to the lower arm portion. A sagittally oriented, elongate slot is aligned with the apex and extends toward the rim of the dive plate. The upper arm portion extends through the elongate slot, and a ballast is associated with the lower arm portion.

Abstract: The selectivity of immunoselection systems employing insolubilized avidin and biotinylated specific antibody is amplified, and nonspecific recovery is improved, by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Specific cell populations can be removed and recovered from bone marrow, providing excellent recovery of bone marrow and preservation of hematopoietic stem cells for transplantation. Mixed populations of T cells or of tumor cells can be conveniently and simultaneously removed with minimal manipulation of the marrow cells. An improved positive immunoselection method provides viable and functional recovered cells, e.g., hematopoietic stem cells or activated killer cells, that can be clinically employed.

Abstract: A process of mammalian gene therapy. Explanted fibroblasts are genetically modified by introducing a retroviral construct containing a nucleotide sequence encoding for a therapeutic substance. The genetically modified fibroblasts are implanted into a mammalian subject.

Abstract: An improvement in a control valve having a body, a body interior portion and plural exterior ports in communication with the interior portion. The improvement includes a plurality of dovetail-like body slots "isolated" or spaced from other slots. Each slot intersects an aperture in fluid communication with the body interior portion. A complementarily shaped, friction-retained slide-by plate is in each slot and at least one plate includes a port in fluid communication with the slot aperture. Each plate slides for removal without disturbing any other plate. A plate may be a blind closure plate, a ported plate tapped with any of several thread sizes or a "back-to-back" interface plate for supportively mounting valve bodies in tandem. A control valve system including a slotted valve body and an assortment of plates of various types provides substantially "universal" porting between the body and another valve body and/or one or more separate control components.

Abstract: The selectivity of immunoselection systems employing insolubilized avidin and biotinylated specific antibody is amplified, and nonspecific recovery is improved, by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Specific cell populations can be removed and recovered from bone marrow, providing excellent recovery of bone marrow and preservation of hematopoietic stem cells for transplantation. Mixed populations of T cells or of tumor cells can be conveniently and simultaneously removed with minimal manipulation of the marrow cells. An improved positive immunoselection method provides viable and functional recovered cells, e.g., hematopoietic stem cells or activated killer cells, that can be clinically employed.

Abstract: An improved method of screening a cell line for the production of a binding partner that binds with a cell-surface molecule, by contacting the binding partner with IL4-activated and nonactivated human bone marrow stromal cells, and selecting binding partners that bind to the IL4-activated human bone marrow stromal cells but not to the nonactivated human bone marrow stromal cells. The selected binding partners may thereafter be tested for the ability to block CD34.sup.+ bone marrow cell binding to IL4-activated human bone marrow stromal cells. The binding partners are preferably also characterized by binding to human VCAM-1. A representative embodiment is mAb 6G10 produced by hybridoma ATTC No. HB 10519.

Abstract: An onco-developmentally regulated .alpha.-N-acetylgalactosaminyltransferase is isolated as a component of a particulate membrane fraction separated from cell and tissue homogenates which has the following characeristics:(a) Activity in Various Cells and Tissues--present in human fetal lung fibroblasts, hepatoma tissues and placenta, but absent from normal adult liver, lung, kidney and spleen tissues;(b) Substrate Specificity--acts on polypeptides comprising a sequence of val-thr-his-pro-gly-tyr by catalyzing .alpha.-N-acetylgalactosaminylation at the thr residue of the sequence;(c) Requirements for Metal Ion--requires metal ion for activity in 25 mM tris buffer, pH 7.6;(d) Optimal pH--optimal pH is about 7.6 asssayed in hepatoma cell homogenate in tris buffer and at a pH of about 6 to about 7 assayed in hepatoma cell homogenate enzyme activity is higher in 2(N-morpholino)ethanelsulfonic acid than in cacodylate buffer; and(e) Km--apparent Km for UDP - GalNAc is about 48 .mu.m.

Abstract: Hybridoma cell lines that produce monoclonal antibodies that differentially recognize glycolipids with mono-, di-, and trifucosylated type 2 chain structures are disclosed. The monoclonal antibodies can be used to detect specific types of tumor cells that are characterized by enrichment in mono-, di-, or trifucosylated type 2 chain structure. As such, the antibodies produced by the hybridoma cell lines are useful for diagnosis and treatment of human cancer. Also disclosed is an improved method of raising hybridoma cell lines by selecting the hybridomas by positive reactively with one or more fucosylated type 2 chain structures selected from the group consisting of III.sup.3 FucnLc.sub.4, V.sup.3 FucnLc.sub.6, III.sup.3 FucnLc.sub.6, III.sup.3 V.sup.3 Fuc.sub.2 nLc.sub.6, and III.sup.3 V.sup.3 VII.sup.3 Fuc.sub.3 nLc.sub.8.

Abstract: A new hybrid tea rose with orange flowers.

Abstract: A new hydrid tea rose with bright red flowers.

Abstract: A new variety of hybrid tea rose having red and silver flowers.

Abstract: A new variety of hybrid tea rose plant having light pink flowers.