Abstract: The invention includes methods for increasing the efficiency of transduction of cells, including non-dividing cells, by recombinant AAV vectors. The methods utilize agents that alter certain aspects of DNA metabolism, more specifically, that affect DNA synthesis and/or affect repair, that impact on maintenance of chromosomal integrity, and/or that cause damage to the cellular DNA. Agents and vectors can now also be preselected and screened for transducing ability and/or transducing agents for their effect on DNA metabolism. These agents include tritiated nucleotides such as thymidine, gamma irradiation, UV irradiation, cis-platinum, etoposide, hydroxyurea and aphidicolin.
Type:
Grant
Filed:
June 6, 1994
Date of Patent:
February 18, 1997
Assignee:
Fred Hutchinson Cancer Research Center
Inventors:
Ian E. Alexander, David W. Russell, A. Dusty Miller
Abstract: The specification discloses methods for modulating cellular metabolism in a subject, modulating being desirable to mitigate a condition of the subject. Disclosed methods include processes for administering to said subject an effective amount of a compound of the formula ##STR1## wherein one and only one of R.sup.1 and R.sup.3 is a straight-chain or branched-chain .omega.-hydroxyalkyl (5-8C), or is a branched-chain (.omega.-1)-hydroxyalkyl (5-8C), or is an (.omega.-1)-oxoalkyl (5-8C), or is an (.omega., .omega.-1) or (.omega.-1, .omega.-2)-dihydroxyalkyl (5-8C), or is an alkenyl substituent (5-8C), and the other is alkyl (1-12C) optionally containing one or two non-adjacent oxygen atoms in place of C.
Type:
Grant
Filed:
January 25, 1995
Date of Patent:
December 17, 1996
Assignee:
Fred Hutchinson Cancer Research Center
Inventors:
James A. Bianco, Stuart L. Bursten, Jack W. Singer
Abstract: Push button controlled wireless transmitters send digitally encoded signals to a plug-in receiver/signaling device. The receiver/signaling device has a microprocessor suitably programmed to respond to signals from a transmitter bearing the discrete address of the receiver, and to decode a transmitter identification code. Thereupon the microprocessor supplies an audio output circuit with signals to simulate selectively mechanical front or back door chimes, and supplies signals to energize a strobe lamp to emit bursts of multiple flashes, the number of flashes in a burst identifying the specific transmitter, whereby a person with a hearing deficiency is informed as to which transmitter is being actuated.
Abstract: An improved fluorescent light ballast circuit extends the on/off life of a florescent lamp within a lighting circuit having a pair of series connected fluorescent lamps. The ballast circuit includes a DC pulse generation circuit, filtering and voltage regulation circuitry, a step-up voltage transformer, and a current altering circuit which alters the current within selected filaments within the lamps. The current altering circuit includes a diode and a coil winding which connect across a filament within one of the fluorescent lamps. The net effect of the current altering circuit is to increase the voltage supplied across the lamp connected to the current altering circuit so that the voltage levels across both lamps are balanced.
Abstract: An avian/mammalian microcell hybrid immortalized pre B cell line containing a mammalian chromosome that carries a selectable marker has been produced. The avian/mammalian microcell hybrid immortalized pre B cell line can be used for introducing predetermined point mutations into specific mammalian chromosomal loci via high frequency homologous recombination.
Type:
Grant
Filed:
March 31, 1995
Date of Patent:
August 6, 1996
Assignee:
Fred Hutchinson Cancer Research Center
Inventors:
R. E. Keith Fournier, Mark T. Groudine, Ellen S. Dieken, Elliot M. Epner
Abstract: An intake manifold for a combustion engine is provided. The intake manifold has a body with a front end, a back end, a top and a bottom. The bottom has a first angled portion, a second angled portion and a horizontal portion located therebetween. The intake manifold further has a front end plate and a back end plate connected to respective ends of the body. The top plate has at least one opening for connecting at least one carburetor system to the intake manifold. The intake manifold further has a first and a second engine mounting plate and a plurality of straight tubes each having a first and a second end. The straight tubes are connected at their first end to the body of the intake manifold and at their second end to the engine mounting plates. The plurality of straight tubes are preferably extruded metal.
Abstract: The present invention relates to cytotoxic T lymphocytes (CTLs) which have been converted to a helper T cell independent phenotype by introducing a recombinant expression vector encoding IL-1 receptor into the CTL. The resulting CTLs have the ability to grow or function independent of T cell help.
Type:
Grant
Filed:
August 8, 1994
Date of Patent:
November 28, 1995
Assignees:
Immunex, Fred Hutchinson Cancer Research Center
Inventors:
Phillip D. Greenberg, Robert W. Overell
Abstract: Method for affecting viability of a eucaryotic cell by contacting the cell with a modulator of the activity of a PIF-1-type helicase in the cell. Such contacting specifically increases or decreases the specific activity of the helicase in the cell.
Abstract: Nucleic acid molecules capable of hybridizing under stringent conditions to the nucleotide sequence residing between positions 1 and 1185 of the human cyclin E cDNA sequence shown in FIG. 2 . Polypeptides encoded by such nucleic acid molecules, and immunologic binding partners directed to such polypeptides.
Type:
Grant
Filed:
September 16, 1992
Date of Patent:
September 12, 1995
Assignee:
Fred Hutchinson Cancer Research Center
Inventors:
James M. Roberts, Motoaki Ohtsubo, Andrew C. Koff, Frederick Cross
Abstract: A sugar stick is provided to stir coffee until a desired sweetness is attained. The dispensing device for solid granular material is user adjusted from zero to a maximum by simply adjusting a movable cover, such as a sleeve or shutter to the indicated extent for the desired amount. The stirrer includes at least one dose separator disks attached to a central portion ending in an enlarged end section to be used as a handle, which fits into a perforated tube. The product granules, e.g. an artificial or natural sweetener, which are larger in size than the perforations, are housed in the spaces between the at least one separator disk. A transparent movable outer cover, such as a sleeve, encloses the perforations and seals them from contact with the liquid beverage. By pulling the movable outer cover up, the perforations corresponding to the desired level of sweetness dose can be easily exposed.
Abstract: A tool for preparing a substrate edge of a counter-top to accept a molded apron having at least two perpendicular appendages. The tool includes cutters for cutting a channel and/or a rabbet into the substrate edge. The spacing between the cutting edges of the cutters corresponds to a spacing between the perpendicular appendages of the molded apron.