Abstract: Disclosed is apparatus (1) for measuring fluorescence and absorbance of a substance in a sample, said apparatus (1) comprising: a flow cell (2) for containing a sample, a first light source (3), a first conductor (5) for transmitting light from the first light source (3) to the flow cell (2) for irradiating a sample contained therein, a second conductor (7) for transmitting light from the flow cell (2) to a sample detector (9) arranged to detect an electromagnetic radiation that has passed through said cell (2), and a processing unit (16) arranged to receive a first signal (31) from a reference detector (15) and a second signal (32) from the sample detector (9) and to determine an absorbance based on said first and second signals (31,32), said apparatus (1) further comprising a second light source (4), a third conductor (6) for transmitting light from the second light source (4) to the cell (2) and wherein the sample detector (9) is further arranged to also detect fluorescence signals in the light that has pa

Abstract: A method and a rotary valve in a continuous chromatography system. The rotary valve comprises a stator with an inner stator face, and a rotor with an inner rotor face arranged in sealing contact with the inner stator face. The stator comprises at least a first and a second inlet orifices, at least two first outlet orifices, a second outlet orifice and a third outlet orifice, and the rotor interconnection paths are arranged to: in at least one rotary position connect the first inlet orifice to the second outlet orifice and the second inlet orifice to the third outlet orifice, and in at least two other rotary positions connect the first inlet orifice with any one of the first outlet orifices at the same time as the second inlet orifice is connected to the second outlet orifice.

Abstract: The present invention relates to a method for manufacturing a device for holding a light guide, for use in a flow cell, with the steps of providing a light guide holder having a first end and a second end connected by a longitudinal through hole with a narrow portion at the first end having a first diameter and a wide portion at the second end having a second diameter, the first diameter being smaller than the second diameter, and the narrow portion and wide portion being connected by a tapering portion, inserting a light guide into the through hole so that a first end of the light guide extends from the first end to the second end of the light guide holder, inserting a substance into at least part of the wide portion of the through hole, Inserting a tube between the light guide and the light guide holder, and fix the light guide in relation to the holder by means of the substance. The invention also relates to a device for holding a light guide.

Abstract: An arrangement for a column comprising a stand provided with a platform adapted to receive a second end of a tubular housing and for supporting the arrangement on a floor, a first end unit removably connected to a first end of the tubular housing, an adaptor assembly movable within said tubular housing and an adaptor rod connected to said adaptor assembly, which adaptor rod is arranged to extend through an opening in the first end unit. A frame is connected to the first end unit, which frame and first end unit can be lifted and lowered in a substantially vertical direction, so that the tubular housing may be removed and substituted for another tubular housing with different dimensions in relation to the removed tubular housing when the frame and first end unit are lifted.

Abstract: A bioreactor system comprising a base station comprising a control system, a tray arranged to be provided on the base station and arranged to house a bioreactor bag, wherein said base station comprises at least one temperature sensor means and in that said tray comprises at least one opening for receiving said temperature sensor means such that it will contact a surface of a bioreactor provided in the tray.

Abstract: A filter heater arranged to enclose a filter, wherein said filter heater comprises two similar halves each having a protruding handle on one side, whereby the handles are kept together by a spring mechanism such that pinching the handles towards each other will force the sides of the two halves opposite the handles from each other.

Abstract: An adaptor for a chromatography column, the adaptor comprising a substantially circular bottom plate provided with an end surface; a ring shaped wall element arranged at the periphery of the bottom plate. The bottom plate and the wall element having a common axis and the adaptor is provided with a filter connection arrangement for removably connecting a filter on the end surface of the bottom plate of the adaptor.

Abstract: A chromatography system comprising at least two chromatography columns, where a feed recirculation of outflow from a primary load column to the inlet of a secondary load column is combined such that the feed recirculation outflow from all columns that will be used as primary load columns in the system will pass through one and the same feed recirculation flow path.

Abstract: In a method of preparing a liquid solution by mixing ingredients according to a predetermined recipe, wherein at least one pair of species of the liquid solution is derived from a weak electrolyte and corresponds to an acid-base pair, the conductivity of the liquid solution is predicted by: (i) for each pair of species derived from a weak electrolyte, solving a respective equilibrium equation to calculate the actual molar concentration of each such species at equilibrium in the liquid solution, (ii) calculating for each ionic species of said plurality of species the molar conductivity by the formula: ?=?0?K×Sqrt(c) wherein ? is the molar conductivity, ?0 is the molar conductivity at infinite dilution, c is the concentration, and K is the Kohlrausch coefficient, and wherein K and ?0 are predetermined values for K and ?0 for each ionic species, (iii) calculating the conductivity ? for each ionic species by the formula: ?=c×? and (iv) adding up the conductivities determined in step (iii) for

Abstract: Methods, systems, and apparatus to monitor component status in a bioprocessing environment are disclosed and described. Certain examples provide a sensor device for a disposable bioprocessing component. The example sensor device includes a first portion affixed to the component, the first portion configured to provide an identifier associated with the component. The example sensor device also includes a second portion configured to provide a status indication based on a state of the component. The example sensor device is configured to transmit the identifier and status indication to a control computer associated with a bioprocessing platform including the component.

Abstract: An apparatus for substantially sterile connection of flexible tubing, including at least one tubing holder, which is adapted to receive one or more lengths of flexible tubing and which is also equipped with one or more sterilant applicators. The sterilant applicator is adapted to apply a sterilant to the length of tubing when the tubing length is received in the tubing holder. At least one cutter, which is movable in relation to the tubing holder and adapted to cut the length of tubing. A connector holder, which includes a compartment housing and a substantially sterile tubing connector. The connector holder is movable in relation to the tubing holder to a position where the length of tubing and the sterile tubing connector are aligned and can be urged together.

Abstract: A method and a slurry tank system for transferring chromatography media slurry from a slurry tank (3) to a chromatography column (9), said method comprising monitoring the content of slurry in the slurry tank (3) during the transferring and controlling the transferring of the slurry to the column (9) in dependence of the content of slurry in the slurry tank (3).

Abstract: A rotary valve 1 comprising a stator 3 and a rotor 5, wherein the stator comprises a plurality of connection ports (17a-17l) and orifices (19a-19l) and the rotor comprises a plurality of pairs of rotor valve orifices (23a-23h) joined by transfer channels (25a-25d). The rotor is able to be placed in different working positions whereby fluids can be fed to, for example, chromatography columns and/or can be bypassed through or around the valve. The invention also relates to a system comprising two components such as chromatography columns and a rotary valve.

Abstract: A chromatography column distribution system includes a set of first bed support ribs extending radially from an inner, first radial position near the center of the plate to an outer radial position nearer to the periphery of the plate and at least one set of intermediate bed support ribs starting at an intermediate radial position and extending to an outer radial position nearer to the periphery of the plate. Channels are formed between adjacent bed support ribs. The desired local effective channel height varies in accordance with a predetermined formula from the first radial position to the outer radial position. The transverse cross-sectional areas of the ribs or the channels are adapted such that the actual local effective channel height is within 15% of the desired local effective channel height over portions of the distribution system situated between the first radial position and the outer radial position.

Abstract: The invention relates to a chromatography column frame comprising at least two legs, a support arrangement connected to said at least two legs, and a holder means connected to said support arrangement. Said holder means is arranged to releasably hold an adaptor rod of a chromatography column, so that the adaptor rod is prevented to move in a horizontal direction and so the adaptor rod is allowed to move in a vertical direction. The invention also relates to method of conducting maintenance on and packing of a chromatography column.

Abstract: Disclosed is an optical fiber arrangement for inducing coupling among propagation modes of light, said arrangement comprising a multimode optical fiber (30) having an input end (32) for receiving light and an output end (31) for emitting light, with a coupling inducing section (33) extending from said input end to said output end, and a holder (80) on which the optical fiber is arranged, wherein said multimode optical fiber has a non-circular cross section. Disclosed also is a system for measuring the absorption or determining the concentration of a substance, said system comprising at least one optical fiber arrangement.

Abstract: The invention discloses an apparatus for aligning and supporting a first and a second length of thermoplastic tubing, preparatory to and during cutting and welding of said lengths of tubing, that includes a first holder arranged to accommodate portions of the first and second lengths of tubing in a first and a second elongated holding space respectively, a second holder arranged to accommodate portions of the first and second lengths of tubing in a third and a fourth elongated holding space respectively, and movable in a direction essentially perpendicular to the holding spaces of the first and second holders from a cutting position, a cutting means arranged between said first and second holders for cutting said lengths of tubing when the second holder is in the cutting position and an aligning means to align these lengths of tubing with each other.

Abstract: The present invention relates to a flow cell (10) comprising a fluid inlet (16) and a fluid outlet (18) separated by a sample flow-through chamber (12) comprising at least one UV-transparent window (22?), wherein the at least one UV-transparent window (22?) is made of a polymer material and has been subjected to Gamma radiation sterilisation. In one aspect, the flow cell is combustible.

Abstract: The invention discloses a method for individually assessing the cleanness of at least one of an upper (2) and a lower (3) bed support in a process column (1). The method comprises the steps of: a) providing a process column (1) comprising an upper (2) and a lower (3) bed support, a column chamber, a movable adaptor (5), an upper outlet (6) and a lower outlet (7); b) conveying liquid through one of the lower (3) and upper (2) bed supports, e.g. by moving the adaptor (5) downwards at a predetermined rate with one of the upper and lower outlets closed and the other open to force liquid through one of the lower and upper bed supports, and; c) measuring the differential pressure over the bed support through which liquid passes.