Abstract: Disclosed herein are methods of performing continuous directed evolution in complex biological systems, including metazoan cells. These methods include the infection of engineered, non-naturally occurring metazoan cells with engineered, non-naturally occurring DNA viruses. The generation of infectious viruses that can infect new cells depends on the evolution of a gene of interest which is driven by an error-prone adenoviral polymerase. Also disclosed herein, are the compositions of engineered, non-naturally occurring metazoan cells and engineered, non-naturally occurring DNA viruses that function as components in the continuous directed evolution methodologies.

Abstract: An optical waveguide 1, an optical waveguide 2 are formed on a substrate 3 to be crossed with each other, modulator electrodes 11, 12, 13 and 14 are arranged along the optical waveguides 1, 2, and antennas 21, 22, 23, 24 (i.e., square patch antennas having an approximately same shape) are arranged around four corners of the square shape. The modulator electrode 11 is energized from the antenna 21 and the antenna 22, the modulator electrode 12 is energized from the antenna 24 and the antenna 23, the modulator electrode 13 is energized from the antenna 21 and the antenna 24, and the modulator electrode 14 is energized from the antenna 22 and the antenna 23. The light wave propagating through the optical waveguide 1 is modulated by an electric field of Y-direction, and the light wave propagating through the optical waveguide 2 is modulated by an electric field of X-direction.

Abstract: The present invention contemplates induction of immunological tolerance thereby providing permanent allograft acceptance. This method obviates the need for a lifelong regimen of immunosuppressive agents which can increase the risk of infection, autoimmunity, and cancer. Immunological tolerance is thought to be mediated by regulatory T lymphocytes (Treg cells) with immunosuppressive capabilities. A therapeutically relevant platform comprising artificial constructs are contemplated comprising numerous soluble and surface bound Treg cell stimulating factors that may induce tolerance following allograft transplantation. Such artificial constructs, being the size of a cell, have surface bound monoclonal antibodies specific to regulatory T-cell surface moieties and encapsulated soluble regulatory T-cell modulating factors.

Abstract: Disclosed is a system for performing key management of an in-vehicle network. The key management system of the in-vehicle network includes a reception unit configured to receive a shared secret key of a central gateway and a domain gateway, a memory configured to store a program for performing key management of the in-vehicle network using the shared secret key, and a processor configured to execute the program. The processor generates a secret key to be stored in a node of the in-vehicle network using the shared secret key and a unique ID of the node.

Abstract: Disclosed is a laser device. The laser device includes a substrate, a pump light source which is disposed on the substrate and provided with a light emitting layer configured to generate pump light, and an upper waveguide which is disposed above the pump light source in a first direction and provided with an upper resonator configured to allow laser light to be generated and resonate by using the pump light.

Abstract: Provided is a dielectric layer that has a rock salt structure in a room temperature stable phase. The dielectric layer is made of a compound having a chemical formula of BexM1-xO, where M includes one of alkaline earth metals and x has a value greater than 0 and not greater than 0.19. A semiconductor memory device also is provided that includes a capacitor composed of a lower electrode; a dielectric layer disposed on the lower electrode; and an upper electrode disposed on the dielectric layer, wherein the dielectric layer has a rocksalt structure in a room temperature stable phase and is made of a compound having a chemical formula shown below, BexM1-xO, where M comprises an alkaline earth metal and x has a value greater than 0 and not greater than 0.19.

Abstract: The purpose of the present invention is to provide an emitter that is made of hafnium carbide (HfC) and that releases electrons in a stable and highly efficient manner, a method for manufacturing the emitter, and an electron gun and electronic device in which the emitter is used. In this nanowire equipped emitter, the nanowires are made of hafnium carbide (HfC) single crystal, the longitudinal direction of the nanowires match the <100> crystal direction of the hafnium carbide single crystal, and the end part of the nanowires through which electrons are to be released comprise the (200) face and the {310} face of the hafnium carbide single crystal, with the (200) face being the center and the {311} faces surrounding the (200) face.

Abstract: The present disclosure provides a quantum dot (QD) light emitting diode including: a first electrode; a second electrode facing the first electrode; a QD emitting material layer positioned between the first electrode and the second electrode and including a QD and an organic material; a hole auxiliary layer positioned between the first electrode and the QD emitting material layer; and an electron auxiliary layer positioned between the QD emitting material layer and the second electrode, wherein the organic material has a highest occupied molecular orbital (HOMO) level higher than a material of the hole auxiliary layer.

Abstract: A method with corresponding systems for reducing emission of amines to the atmosphere. The method includes a a) introducing a gas containing CO2 into an absorber; b) flowing the flue gas through an absorber having an absorbent with a water-lean solution having less than 50% water and one or more amines, with the absorbent capturing the CO2 and forming a reduced CO2 content gas having a baseline CO2 content; and c) washing the reduced CO2 content gas in a wash column with a wash solution comprising carbonic acid formed by addition of gaseous CO2 into the wash solution. In this method, the washing removes the amines from the reduced CO2 content gas and produces a reduced amine content gas exiting from the wash column.

Abstract: A polymer or other substrate optimized for growing cells is described, which takes the form of a micro-thin bag with gas permeable sides. Sides of the bag can be held at a fixed distance from one another with a multitude of tiny micropillars or other spacers extending between them, keeping the bag at a predetermined thickness and preventing the bag from collapsing and the sides from sticking together. In other embodiments, the sides may be held apart by gas pressure alone. A 0.01 ?m to 1000 ?m parylene or other biocompatible coating over the bag outsides controls the permeability of the bag material and provides a bio-safe area for cell growth. An alternate configuration uses open-cell foam with skins coated with a biocompatible coating. Tubes going into multiple bags can be connected to a manifold that delivers gaseous oxygen or removes carbon dioxide and other waste gases.

Abstract: Aminoalcohol lipidoids are prepared by reacting an amine with an epoxide-terminated compound are described. Methods of preparing aminoalcohol lipidoids from commercially available starting materials are also provided. Aminoalcohol lipidoids may be prepared from racemic or stereochemically pure epoxides. Aminoalcohol lipidoids or salts forms thereof are preferably biodegradable and biocompatible and may be used in a variety of drug delivery systems. Given the amino moiety of these aminoalcohol lipidoid compounds, they are particularly suited for the delivery of polynucleotides. Complexes, micelles, liposomes or particles containing the inventive lipidoids and polynucleotide have been prepared. The inventive lipidoids may also be used in preparing microparticles for drug delivery. They are particularly useful in delivering labile agents given their ability to buffer the pH of their surroundings.

Abstract: An ice protection system and method capable of anti-icing and de-icing aerodynamic structures and surfaces is provided comprising a Resistive Heat Coat (RHC) controller and a heating device. The RHC controller comprises a RHC power circuit topology having a processor and a buck converter. The RHC controller further comprises a RHC control algorithm. The heating device comprises a plurality of resistive heating elements, such as CNT-based resistive heaters. The ice protection systems and methods disclosed can achieve an efficiency of 98% or greater while employing a direct current (DC) power supply and “hard switching” with a switching frequency of at least 500 kHz.

Abstract: A large reflector inflatable telescope. The telescope has an inflatable hull with a conical lower portion and a hemispherical upper portion. The conical portion is transparent to electromagnetic waves so that radio or light waves can reach an inner reflector, which is interposed between the conical portion and the hemispherical portion. The presence of the inner reflector allows the conical portion and the hemispherical portion to be inflated at different pressures such that the reflector becomes spherical or parabolic. If desired, the reflector can be made adjustable with a membrane behind the reflector that provides electro-static force to shape the reflector.

Abstract: Described is a lattice cryptography processor with configurable parameters. The lattice cryptography processor includes a sampling circuit configured to operate in accordance with a Secure Hash Algorithm 3 (SHA-3)-based pseudo-random number generator (PRNG), a single-port random access memory (RAM)-based number theoretic transform (NTT) memory architecture and a modular arithmetic unit. The described lattice cryptography processor is configured to be programmed with custom instructions for polynomial arithmetic and sampling. The configurable lattice cryptography processor may operate with lattice-based CCA-secure key encapsulation and a variety of different lattice-based protocols including, but not limited to: Frodo, NewHope, qTESLA, CRYSTALS-Kyber and CRYSTALS-Dilithium, achieving up to an order of magnitude improvement in performance and energy-efficiency compared to state-of-the-art hardware implementations.

Abstract: Provided herein are methods for generating single-cell molecular analysis comprising a) delivering one or more proximity dependent probes to a cell population, wherein each proximity dependent probe comprises a target binding region configured to bind a target RNA and a primer binding site region; b) linking bound proximity dependent probes; c) isolating single cells from the cell population in separate individual discrete volumes, the individual discrete volumes further comprising a primer pair and amplification reagents, wherein the primer pair binds to the primer binding sites of the ligation dependent probes, and wherein at least one primer comprises a barcode sequence that uniquely identifies the individual discrete volume; d) amplifying the ligated probes using the primer pair, wherein the barcode is incorporated into each resulting amplicon; and e) quantifying target RNAs in each individual cell based at least in part on sequencing the resulting amplicons.

Abstract: A lithium-manganese composite oxide represented by formula (1): Li1+x[(FeyNi1?y)zMn1?z]1?xO2 (1) wherein x, y, and z satisfy the following: 0<x??, 0?y<1.0, and 0<z?0.6, and wherein the content ratio of Li to the total content of Fe, Ni, and Mn (Li/(Fe+Ni+Mn)) is 1.55 or less on a molar ratio basis, the lithium-manganese composite oxide containing a crystalline phase having a layered rock-salt structure. This composite oxide is a novel material made from less resource-constrained and cheaper elements, and exhibits a high specific capacity, excellent charge-and-discharge cycle characteristics, and a high discharge capacity at a high current density (excellent rate characteristics), when used in the positive electrode material for lithium-ion secondary batteries.

Abstract: A system for transitioning a vehicle from an autonomous mode in response to a handover event, the system includes one or more processors and a memory device operably coupled with the one or more processors. The memory device stores a driver assessment module, an operational condition adjustment module, and a transitioning module. The driver assessment module configures the one or more processors to determine an ability level and a comfort level of a driver of the vehicle in response to the handover event. The operational condition adjustment module configures the one or more to adjust at least one operating condition of the vehicle to be within the ability level of the driver and comfort level. The transitioning module configures the one or more processors to transition the vehicle from the autonomous mode to a driver input mode.

Abstract: A method of detecting bio-material includes preparing an electrode where capture structures are fixed on surface of the electrode; preparing nanoparticles where probes are fixed on surfaces of the nanoparticles; providing bio-materials on the nanoparticles so that the probes of a portion of the nanoparticles make complementary bonds with the bio-materials, respectively, to form composites, and another portion of the nanoparticles remain without making bonds with the bio-materials; and providing another portion of the remaining nanoparticles on the surface of the electrode so that the capture structures make complementary bonds with the probes of another portion of the nanoparticles, respectively, wherein each of the capture structures includes nucleotides having first sequence, each of the probes includes nucleotides having second sequence, each of the bio-materials includes nucleotides having third sequence, the first and second sequences are complementary to each other, and the second and third sequences ar

Abstract: Disclosed are monoacylated Toll-like receptor 2 ligands which can be used in both the development of targeted agents for the imaging and treatment of pancreatic cancer as well as other cancers, and as an adjuvant for cancer immunotherapy. The monoacylated compounds disclosed herein have a higher binding affinity for TLR2 relative to a known potent diacylated agonists, but only ?½ the bioactivity. Competition of the monoacylated compound with the diacylated compound for binding TLR2 was confirmed. Hence, the reported monoacylated compounds are inhibitors/antagonists of TLR2 activation.

Abstract: A tetracarbonyl cyclobutene dihydrate compound, having a chemical formula (I). A method of synthesizing the tetracarbonyl cyclobutene dihydrate compound, includes: a synthesis step, a separation step, and a purification step. The synthesis step includes: collecting and dissolving 0.5728 g of squaric acid, 2.7948 g of ammonium formate, and 0.0480 g of the palladium complex in 100 mL of anhydrous methanol, heating and stirring a resulting mixture to reflux for 48 hrs, and stopping the reaction. The separation step includes: performing column chromatography analysis on reaction products according to a volume ratio of dichloromethane to anhydrous methanol of 8:2, to obtain a target product. A method of using the tetracarbonyl cyclobutene dihydrate compound, includes: using tetracarbonyl cyclobutene dihydrate compound as a catalyst in addition reaction between ethyl pyruvate and nitromethane, where a conversion rate of ethyl pyruvate reaches 96.1%.