Patents Assigned to JIANGNAN UNIVERSITY
  • Patent number: 12168793
    Abstract: A method for enzymatically preparing a n-3 polyunsaturated fatty acid diacylglycerol using oil rich in n-3 polyunsaturated fatty acid as raw material to prepare diacylglycerol oil with selective enzymatic hydrolysis. Before the enzymatic reaction, the raw materials are homogenized to improve the catalytic efficiency of lipase in the hydrolysis and achieve the purpose of reducing the reaction time and the addition amount of lipase. As a result, the cost of industrial diacylglycerol preparation is reduced, ultimately reducing the cost of industrial diacylglycerol preparation. Therefore, the present invention provides an effective, mild and low-cost enzymatic method for preparing diacylglycerol rich in n-3 polyunsaturated fatty acids, which has a promising potential for industrial application.
    Type: Grant
    Filed: May 15, 2023
    Date of Patent: December 17, 2024
    Assignee: Jiangnan University
    Inventors: Jialing Gu, Xiaosan Wang, Houyue Li, Zhimian Liu, Weijia Bao, Zixin Wang, Yifan Wang, Yijie Fu, Yujie Su
  • Patent number: 12163121
    Abstract: The present invention provides a mutant strain of Corynebacterium glutamicum with efficient expression of exogenous proteins, which can solve the technical problem of low protein expression quantity of using Corynebacterium glutamicum as an exogenous protein expression host. The mutant strain of Corynebacterium glutamicum is deposited in the China General Microbiological Culture Collection Center (CGMCC), and the deposit number is CGMCC No. 20138. The mutant strain of Corynebacterium glutamicum in the present invention, verified by the expression of exogenous proteins, shows a significantly enhanced expression of both intracellular and secreted proteins when compared with its initial strain.
    Type: Grant
    Filed: July 30, 2021
    Date of Patent: December 10, 2024
    Assignee: Jiangnan University
    Inventors: Xiuxia Liu, Lihong Meng, Yankun Yang, Chunli Liu, Ye Li, Zhonghu Bai
  • Patent number: 12152245
    Abstract: The present invention provides a nucleic acid aptamer specifically recognizing ?-lactoglobulin and use thereof. The nucleic acid aptamer has a sequence as shown in SEQ ID NO:1, a sequence having 60% or higher homology to the sequence as shown in SEQ ID NO:1 and specifically recognizing ?-lactoglobulin, or a sequence derived from the sequence as shown in SEQ ID NO:1 and specifically recognizing ?-lactoglobulin. The nucleic acid aptamer specifically binds to the allergen ?-lactoglobulin in cow milk and dairy products, thereby providing a new tool for the high-sensitivity and low-cost detection of the allergen ?-lactoglobulin.
    Type: Grant
    Filed: June 24, 2020
    Date of Patent: November 26, 2024
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Nuo Duan, Shijia Wu, Shuo Qi, Zhouping Wang
  • Patent number: 12144363
    Abstract: The present application belongs to the technical field of research and development of fermented beverages, and a method for preparing Cordyceps militaris ferment by two-stage fermentation and complex enzymatic hydrolysis is provided. The present application involves preparation of a Cordyceps militaris powder and a Cordyceps militaris slurry, preparation of a Cordyceps militaris fermentation substrate, lactic acid bacteria fermentation in combination with complex enzymatic hydrolysis, and yeast fermentation, followed by centrifugation, filtration, formulation, sterilization, and filling to obtain a product. By employing lactic acid bacteria fermentation in combination with complex enzymatic hydrolysis, and secondary fermentation by yeast, the present application can quickly finish a whole fermentation process in 2 to 3 days.
    Type: Grant
    Filed: December 6, 2019
    Date of Patent: November 19, 2024
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Min Zhang, Yanyan Lao, Wuxiong Yang, Sifu Yi, Lihua Hou, Xiaoling Guo
  • Patent number: 12145866
    Abstract: The disclosure provides a modified cyclodextrin/mesoporous silica for adsorbing Pb and Cd and application thereof, belonging to the technical field of adsorbent materials. By using surface modification, chloroacetic acid is used as anions, and a modified cyclodextrin is grafted onto a surface of a cyclodextrin/mesoporous silica by nucleophilic substitution to prepare the modified cyclodextrin/mesoporous silica adsorbent material. The modified cyclodextrin/mesoporous silica adsorbent material prepared in the disclosure has the advantages of simple preparation method, strong adsorbability, easy separation, good biocompatibility and the like. When the material is used as an adsorbent to adsorb heavy metal ions Pb2+ and Cd2+, maximum removal rates can reach 97.8% and 81.29% respectively. Therefore, the material has wide application prospects in removal of heavy metals in sewage and the like, thereby improving the water environment that people depend on for survival.
    Type: Grant
    Filed: August 16, 2021
    Date of Patent: November 19, 2024
    Assignee: Jiangnan University
    Inventors: Jinpeng Wang, Jing Li, Zhengyu Jin, Xueming Xu, Yaoqi Tian, Jie Long, Xing Zhou, Zhengjun Xie
  • Patent number: 12142705
    Abstract: The disclosure relates to a method for regulating photocurrent of IGZO based on a two-dimensional black phosphorus material, and belongs to the field of semiconductor devices. The disclosure provides a method for preparing an IGZO-black phosphorus heterostructure by dry transfer technology, and by changing the contact mode between IGZO and black phosphorus, photocurrent response of the IGZO to different laser light wavelengths can be regulated. In the method, both a channel and electrodes of the IGZO are magnetron sputtered by means of masks, and the method has good repeatability and can realize preparation of large-area multi-devices. A black phosphorus sample is prepared by a mechanical exfoliation method, and has controllable thickness and size. A heterojunction is prepared by dry transfer technology, and the technology is easy to operate and highly controllable. The disclosure is beneficial to promote development of IGZO thin films in the micro-nano field and the semiconductor industry.
    Type: Grant
    Filed: May 31, 2022
    Date of Patent: November 12, 2024
    Assignee: Jiangnan University
    Inventors: Haiyan Nan, Daqing Li, Feng Shao, Shaoqing Xiao, Xiaofeng Gu
  • Patent number: 12133887
    Abstract: The disclosure discloses a chemical synthesis method of oligosaccharides of a Pseudomonas aeruginosa serotype O11 O-antigen, and belongs to the field of chemistry. The disclosure includes constructing an O-antigen trisaccharide by using a D-glucose building block, an L-fucosamine building block and a D-fucosamine building block; wherein the D-glucose building block or the L-fucosamine building block is linked with the D-fucosamine building block through a 1,2-?-cis-glycosidic bond, the D-glucose building block is linked with the L-fucosamine building block through a 1,2-?-trans-glycosidic bond, and the construction of the 1,2-?-cis-glycosidic bond is conducted in a mixed solvent; and the mixed solvent includes two or more of dichloromethane, diethyl ether and thiophene.
    Type: Grant
    Filed: September 11, 2020
    Date of Patent: November 5, 2024
    Assignee: Jiangnan University
    Inventors: Jian Yin, Jing Hu, Zhonghua Liu, Chunjun Qin
  • Patent number: 12129219
    Abstract: Provided is a calcium-based catalyst and a method for catalytically synthesizing an alkanolamide surfactant thereof. The method includes the preparation of the catalyst, and then catalyzing a reaction of fat and oil, or fatty acid ester and alkanolamine with the catalyst to synthesize a light colored alkanolamide surfactant with less free alkanolamine. The catalyst has a slow release feature, so that late in the reaction, the catalyst still has relatively high catalytic reactivity, guaranteeing a high conversion rate of the alkanolamine into the alkanolamide surfactant, the, product residual quantity of alkanolamine is low, while the risk of nitrosamine production due to product residual alkanolamine is greatly lowered. Due to catalyst slow release control, the early reaction period speed is greatly reduced, thereby avoiding a deepened product color caused by poor heat transfer due to an early reaction period being excessively fast.
    Type: Grant
    Filed: December 7, 2020
    Date of Patent: October 29, 2024
    Assignee: Jiangnan University
    Inventors: Yun Fang, Xueyi Hu, Yongmei Xia
  • Patent number: 12127564
    Abstract: Disclosed are a preparation method and application of flour with low oil absorption during frying, belonging to the field of flour modification. The preparation method of flour with low oil absorption during frying increases the cross-linking degree of starch, protein, and starch-protein in the flour by adding glutamine transaminase first and then phytic acid or adding phytic acid first and then glutamine transaminase so as to improve the compactness of the flour structure, which reduces water evaporation and oil absorption during frying after the flour is made into dough, and thus ultimately forms the modified flour with low oil absorption during frying. The method is simple, green, pollution-free, and low in energy consumption, and the modified flour prepared has improved thermal stability and reduced oil absorption after frying. The present disclosure has broad application prospects in the fields of flour modification and fried foods.
    Type: Grant
    Filed: April 22, 2024
    Date of Patent: October 29, 2024
    Assignee: Jiangnan University
    Inventors: Long Chen, Yuanhui Chen, Zhengyu Jin, Yaoqi Tian, Ming Miao, Jianwei Zhao
  • Patent number: 12129505
    Abstract: Disclosed is oleaginous yeast and its application, belonging to the technical field of microorganisms. The oleaginous yeast provided by the disclosure has been deposited in China Center for Type Culture Collection on May 21, 2020, with an accession number of CCTCC NO: M 2020139. The oleaginous yeast provided by the disclosure can use a hydrolysate of cheap lignocellulose biomass as the substrate. Xylose and glucose in the hydrolysate are simultaneously utilized to produce oil, so the sugar utilization efficiency and production intensity are enhanced. After 8 days of fed-batch fermentation, the oil yield can reach 31.33 g/L, and the oil content can reach 60.83%. The waste resources can be reutilized, and the production cost is reduced, so the oleaginous yeast has wide application prospects.
    Type: Grant
    Filed: February 18, 2022
    Date of Patent: October 29, 2024
    Assignee: Jiangnan University
    Inventors: Fubao Sun, Qiuli Zhou, Yun Hu, Hongyan Ren, Chihe Sun
  • Patent number: 12129504
    Abstract: The disclosure discloses Oscillospiraceae sp. LBM10036 capable of utilizing multiple substrates and application thereof, and belongs to the fields of microbial technology and Baijiu brewing. The microorganism Oscillospiraceae sp. LBM10036 of the disclosure was preserved in the Guangdong Microbial Culture Collection Center on Sep. 21, 2020, with the preservation number GDMCC No. 61202. The Oscillospiraceae sp. LBM10036 of the disclosure has the capability to utilize diverse substrates and high product specificity, adapts to low pH, and can efficiently synthesize caproic acid under a pH of 5.0-5.5. The strain used in the disclosure can be used for strengthening microorganisms in pit mud, and improving the quality of degraded pit mud, to improve the quality of Strong aroma-type Baijiu, and also can be used for producing caproic acid from a variety of sugars and lactic acid by microbial fermentation.
    Type: Grant
    Filed: September 27, 2023
    Date of Patent: October 29, 2024
    Assignee: Jiangnan University
    Inventors: Yan Xu, Cong Ren, Huilin Wang
  • Patent number: 12131665
    Abstract: The disclosure discloses a bionic digestive tract as well as a preparation method and application thereof, belonging to the field of bionic technologies and the field of biological technologies. The bionic digestive tract of the disclosure is prepared by mixing a base material (one or more of silica gel, latex and hydrogel) and auxiliary materials (silicone oil and a curing agent) in a certain mass ratio (the mass ratio of the base material to the silicone oil to the curing agent is 100:(0.5 to 10):(0.5 to 3.5)). The simulation performance of the bionic digestive tract is excellent, has strong consistency with a true human digestive tract in terms of performance, structure and function, can simulate the true states of food, drugs and microorganisms in a digestive system, and has great application prospects in the research process of food and drugs.
    Type: Grant
    Filed: May 30, 2023
    Date of Patent: October 29, 2024
    Assignee: Jiangnan University
    Inventors: Minjie Gao, Zhitao Li, Xiaobei Zhan, Li Zhu, Wenlong Zhang, Jingjing Xu, Yun Jiang
  • Patent number: 12128111
    Abstract: The disclosure discloses a glyco-metal-organic frameworks-based hepatic targeted therapeutic drug and a preparation method thereof, and belongs to the field of biomedicine. The disclosure loads a chemotherapeutic drug onto specific metal-organic frameworks, and modifies targeted molecule galactose on the surface of the materials through amide reaction. The biocompatibility and cytotoxicity of the obtained hepatic targeted therapeutic drug have been carefully evaluated at the cellular level. The hepatic targeted therapeutic drug of the disclosure has good stability and acidic pH triggered drug release property, and can exert the synergistic therapeutic effect of photodynamic therapy and chemotherapy. In addition, in vivo behavioral tracing and therapeutic efficacy are evaluated in mouse models with subcutaneous solid tumor and tumor in situ, and the disclosure is expected to play a huge role in clinical applications.
    Type: Grant
    Filed: June 11, 2021
    Date of Patent: October 29, 2024
    Assignee: Jiangnan University
    Inventors: Jing Hu, Jian Yin, Jun Hu
  • Patent number: 12123007
    Abstract: The present disclosure discloses a method for constructing an efficient Bacillus subtilis promoter, and belongs to the technical field of gene engineering. According to the present disclosure, natural promoters identified by different sigma subunits are connected in series to obtain some double-series and triple-series promoters, the lengths of intervening sequences between core areas of the promoters are optimized on the basis of series connection of the promoters to further improve the activity of the promoters, finally, different RBS designs are performed on the promoters, and it is verified that this strategy can not only improve the compatibility between the promoters and other gene expression regulating and controlling elements, but also controllably regulate the expression of exogenous genes.
    Type: Grant
    Filed: January 28, 2021
    Date of Patent: October 22, 2024
    Assignee: Jiangnan University
    Inventors: Zhemin Zhou, Wenjing Cui, Laichuang Han, Zhongmei Liu, Li Zhou, Junling Guo, Yu Yan
  • Patent number: 12123029
    Abstract: The present invention discloses a cyclohexenecarboxylate ester hydrolase, and a mutant, a coding gene, an expression vector, recombinant bacterium and use thereof. The cyclohexenecarboxylate ester hydrolase AcEst1 and its mutant of the present invention have the function of enantioselectively resolving methyl 3-cyclohexene-1-carboxylate with high efficiency to prepare optically active (S)-3-cyclohexene-1-carboxylic acid. When the substrate concentration is as high as 2000 mM (about 280 g/L), the optical purity of the product is higher than 99%, and the substrate/catalyst is as high as 3500 g/g. As compared with other preparation methods, the product prepared by the method of the present invention has high concentration and high optical purity, the catalytic efficiency is high, the reaction conditions are mild. Furthermore, the method is environmentally friendly, simple in operation and easy for industrial scale-up, thus has a good prospect of application in industry.
    Type: Grant
    Filed: July 30, 2020
    Date of Patent: October 22, 2024
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Ye Ni, Zhe Dou, Guochao Xu
  • Patent number: 12123037
    Abstract: Disclosed is linoleic acid isomerases and their application in production of conjugated linoleic acid, which belongs to the technical fields of protein engineering and microbial engineering. The linoleic acid isomerase derived from Bifidobacterium is used to produce the conjugated linoleic acid. The recombinant E. coli containing the linoleic acid isomerase of the invention is added into a reaction system containing linoleic acid and react for 3 h to produce conjugated linoleic acids. The conversion rate of the conjugated linoleic acid of the invented method ranges from 12.1% to 42.1%, and the percentage of cis9, trans11-CLA in the conjugated linoleic acid can reach 84.3% to 89.1%. The invention provides a method for using microorganisms to produce conjugated linoleic acids with high safety and yield where cis9, trans11-CLA isomer is the major form in the conjugated linoleic acid products.
    Type: Grant
    Filed: October 10, 2021
    Date of Patent: October 22, 2024
    Assignee: Jiangnan University
    Inventors: Haiqin Chen, Bo Yang, He Gao, Jianxin Zhao, Yongquan Chen, Hao Zhang, Wei Chen
  • Patent number: 12123134
    Abstract: The present disclosure discloses application of a deep eutectic solvent in textile dyeing, belonging to the technical field of ecological dyeing and finishing of textiles. A natural hydrophobic deep eutectic solvent system (HDES) is first used as a dyeing medium to perform reactive dyeing on cotton. Firstly, the prepared natural hydrophobic deep eutectic solvent is mixed with a small amount of dye aqueous solution at high speed to prepare dye liquor, and then cotton fabric soaked with alkali liquor is put into a dye bath for dyeing. The dyeing method doesn't require addition of inorganic salts and other dyeing auxiliaries, but uses a small amount of water and alkali agent to achieve the same dyeing effect as traditional water bath, and the dyeing medium is safe and environment-friendly. After dyeing, cyclic dyeing can be achieved by simply separating the dye bath, which truly realizes green dyeing of reactive dyes.
    Type: Grant
    Filed: February 7, 2023
    Date of Patent: October 22, 2024
    Assignee: Jiangnan University
    Inventors: Qiang Wang, Yimin Wei, Ping Wang, Yuanyuan Yu, Man Zhou, Zhe Jiang
  • Patent number: 12123039
    Abstract: The disclosure discloses the preparation of thermophilic ?-glucosidase and the application thereof, which belongs to the technical field of genetic engineering and fermentation engineering. The present disclosure heterologously expresses the ?-glucosidase TpBgl3A derived from thermophilic fungus Talaromyces piceae by constructing a recombinant bacteria. The enzyme production of recombinant bacteria can reach 2324 U/mL in a 3.6 L fermenter. The resulting ?-glucosidase TpBgl3A can produce gentioligosaccharides with a high conversion rate using glucose as a substrate at a lower enzyme amount added, which significantly reduces production costs. In the reaction system using glucose and cellobiose as substrates, the conversion rate of gentioligosaccharide reaches 26.2%, which has the potential for industrial production of gentioligosaccharide.
    Type: Grant
    Filed: November 8, 2021
    Date of Patent: October 22, 2024
    Assignee: Jiangnan University
    Inventors: Jing Wu, Wei Xia, Xinghao Xu, Yan Huang
  • Patent number: 12118896
    Abstract: The present invention discloses a visual bionic digestive system for a human gastrointestinal tract model, and belongs to the field of bionic digestive systems.
    Type: Grant
    Filed: September 5, 2018
    Date of Patent: October 15, 2024
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Xiaobei Zhan, Zhitao Li, Minjie Gao, Wenlong Zhang, Zhiyong Zheng, Xingqiao Peng, Yun Jiang
  • Patent number: 12116455
    Abstract: The disclosure discloses preparation and application of an alkylcyclohexanol polyoxyethylene ether emulsifier, and belongs to the technical field of surfactants. By performing ethylene oxide adducting on alkylcyclohexanol polyoxyethylene ether (1-3) and using a strong alkaline suspension dispersed in the solvent and alkylcyclohexanol polyoxyethylene ether (1-3) as a catalyst, nonionic surfactants alkylcyclohexanol polyoxyethylene ether (5-17) are synthesized. The products all have good characteristics of nonionic surfactants, and contain lower content of polyethylene. The products such as nonylcyclohexanol ethoxylate (7) and nonylcyclohexanol ethoxylate (9) have emulsifying properties similar to the emulsifying property of nonylphenol ethoxylate (10), and therefore can substitute for nonylphenol ethoxylate (10) as an emulsifier.
    Type: Grant
    Filed: December 7, 2020
    Date of Patent: October 15, 2024
    Assignee: Jiangnan University
    Inventors: Yongmei Xia, Yun Fang, Xueyi Hu, Conglin Ren, Fei Qian, Yitao Hu, Xinyu Meng