Abstract: The present disclosure discloses a salt-reduced fermentation method for high-salt dilute-state fermented soy sauce, and belongs to the technical field of fermentation engineering. The present disclosure separates and screens Weissella paramesenteroides JL-5 and Bacillus amyloliquefaciens JDF-2 which inhibit the growth of spoilage bacteria from low-salt soy sauce mash, and by changing the microbial fermentation process of high-salt dilute-state soy sauce, the strains obtained by screening are used for the fermentation of low-salt soy sauce mash. Without changing the flavor and quality of soy sauce, low-salt fermentation of the soy sauce is enabled to proceed normally, and the biogenic amine content is less than 100 mg L?1, and the number of spoilage bacteria in the soy sauce are reduced. The prepared low-salt soy sauce has an amino acid nitrogen content of greater than 1.2 g·100 mL?1, contents of various spoilage bacteria of less than 1.

Abstract: The present invention provides a knowledge reuse-based method and system for predicting a cell concentration in a fermentation process. The method includes: constructing a cell concentration soft sensor universal model in a fermentation process; acquiring and preprocessing process data of a fermentation stage A; determining a cell concentration soft sensor model of the fermentation stage A; designing a cell concentration online soft sensor of a fermentation stage B; and predicting a cell concentration of the fermentation stage B according to the cell concentration online soft sensor of the fermentation stage B. The present invention resolves the problems of weak generalization of a cell concentration soft sensor model and high costs of establishing models for fermentation stages separately, thereby improving the prediction accuracy of a cell concentration soft sensor.

Abstract: Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86th site, the 373rd site, the 374th site, the 496th site, the 507th site, or the 747th site of agarase with an amino acid sequence as shown in SEQ ID NO. 1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.

Abstract: The disclosure discloses an alginate lyase and application thereof, and belongs to the technical field of biology. The alginate lyase provided by the disclosure has high degradation activity, and the enzyme activity reaches 65 U/mg; the alginate lyase is stable in nature, and the enzyme activity remains 98% or higher of the initial enzyme activity after storage at 4° C. for 18 months; and the alginate lyase has high product specificity. The disclosure uses E. coli as a host to express the alginate lyase derived from V. natriegens, the obtained recombinant E. coli can produce the alginate lyase secreted extracellularly in a conventional LB medium without adding an induction substrate sodium alginate, so the downstream processing technology of protein is simplified, and the disclosure has great industrial application potential.

Abstract: This application relates to a yarn carrier device with a yarn tension control function for a flat knitting machine, and a yarn tension control method. At the moment a carriage is connected to a yarn carrier device, a fixing frame carrying a bobbin ascends or descends on sliding rails installed on the yarn carrier device. The ascending or descending speed, time and distance of the fixing frame can be calculated directly according to a yarn carrier stop, the quantity of an unknitted portion of the yarn and the speed of the carriage. When the carriage contacts a yarn carrier, the fixing frame is controlled to ascend along the sliding rails. When the carriage drives the yarn carrier to enter a knitting area, the fixing frame is controlled to descend.

Abstract: The present disclosure provides a preparation method of eucalyptol emulsion and application thereof in biopesticides, belonging to the field of emulsion preparation. The method for preparing the eucalyptol emulsion includes the steps of: (1) adding eucalyptol into a cyclodextrin solution to obtain a mixed solution; (2) evenly mixing an emulsifier and a co-emulsifier to obtain a mixed solution; (3) adding the solution obtained in step (2) and water into the solution obtained in step (1), emulsifying to obtain the eucalyptol emulsion. In steps (1), (2) and (3), the mass percents of substances from which the eucalyptol emulsion is prepared are as follows: 0.5%-1% of cyclodextrin, 2.5%-25% of the eucalyptol, 1.25%-15% of the emulsifier, 0.3%-3.75% of the co-emulsifier and 55.3%-95.5% of the water, and the sum thereof is 100%. The emulsion is extremely stable without stratification after being placed at 20° C., 40° C. and 60° C. for 30 days and also has a good insecticidal effect.

Abstract: The disclosure discloses stabilized rice aleurone and a preparation method, using the technology of classifying a rice aleurone layer by a tangential injection airflow impeller classifier combined with an ultrasonic vibrating screen for the first time, and simultaneously uses steam treatment coupled with thermal enzyme inactivation by drum drying as a stabilization method to prepare the stabilized rice aleurone for the first time. The drum drying equipment realizes the two purposes of enzyme inactivation and drying, and the method has the advantages of short process flow, simple equipment, high utilization rate, small floor space, low investment, low energy consumption and no pollution, and is suitable for industrial promotion. The stabilized rice aleurone has high nutritional value, is rich in functional ingredients, and can be used as a raw material for functional food development in industrial production and food applications.

Abstract: The present disclosure discloses a high-temperature fast-curing starch-based adhesive for particleboards and a preparation method thereof, belonging to the technical field of adhesive preparation. The low viscosity of the starch-based adhesive is ensured by selecting a crosslinking monomer which does not self-crosslink in a reaction process in the present disclosure, and a binary crosslinking agent matched with the crosslinking monomer is added before use to be quickly crosslinked with the crosslinking monomer at high temperature so as to ensure better thermosetting property and water resistance as well as faster curing speed of the starch-based adhesive at the same time, which meets the requirements of the particleboards for the adhesive, solves the problem of long curing time of the existing starch-based adhesives at high temperatures, further shortens the curing time of the starch-based adhesives to about 60 s, and improves the production efficiency of the particleboards.

Abstract: The invention provides a recombinant Escherichia coli strain for producing succinic acid and a construction method thereof. The by-product encoding genes in the E. coli strain FMME-N-2 are knocked out to obtain the E. coli strain FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA); and the phosphoenolpyruvate carboxykinase pck derived from Actinobacillus succinogenes and the phosphite dehydrogenase ptxD derived from Pseudomonas stutzeri were overexpressed. The constructed plasmid pTrcHisA-pck-ptxD was introduced into the expression host E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA), and the cells were screened in a plate containing ampicillin, to obtain an engineered strain E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA)-pck-ptxD that can efficiently produce succinic acid. After fermentation by a two-stage fermentation strategy, the production of succinic acid reaches 137 g/L, the yield of succinic acid is up to 1 g/g glucose, and the space time yield is 1.

Abstract: The disclosure discloses an ultrasonic-assisted pretreatment method for extraction of multiple steroid hormones in a sediment, including the following steps: (1) lyophilizing the sediment, grinding the sediment, and passing the ground sediment through a 40-60-mesh sieve; (2) placing the sample obtained in step (1) in a container; (3) adding an extractant to the container in step (2), shaking the mixture for 15 s-30 s, centrifuging the mixture to collect an supernatant after ultrasonication, and repeating extraction three times; where the extractants used in the three times of extraction are two of methanol, acetonitrile and acetone; and (4) after mixing the supernatants of the three times of extraction obtained in step (3), concentrating the mixture under a nitrogen flow at 20-30° C., passing the concentrated mixture through a filter, and performing detection. By using the method of the disclosure, the maximum recovery can be up to 100%.

Abstract: The present disclosure discloses a strain producing D-allulose 3-epimerase and application thereof, and belongs to the technical field of bioengineering. The present disclosure provides a method for improving the expression of D-allulose 3-epimerase by screening promoters and optimizing RBS thereof. The recombinant Bacillus subtilis constructed using thevectors pP43NMK-hag and pP43NMK-hag-RBS4 provided by the present disclosure improves the enzyme activity of a target gene D-allulose 3-epimerase, and theenzyme activities in shake flasks upon transformation are 1.30 times and 1.69 times that of an original vector. The present disclosure further provides a non-antibiotic resistance vector and a non-antibiotic resistance recombinant B. subtilis strain. Using the non-antibiotic resistance strain B. subtilis 1A751-dal-/pP43NMK-hag-RBS4-dpe-dal provided by the present disclosure, the highest fermentation enzyme activity in a shake flask is 24.72 U/mL, and the enzyme activity in a fermenter is 714.8 U/mL.

Abstract: The disclosure discloses an irreversible covalent organic framework for efficient and selective gold recovery and a preparation method thereof, and belongs to the technical field of precious metal recovery from an aqueous solution. In the disclosure, metal trifluoromethanesulfonate is used as a catalyst, and a solvothermal method is used to prepare a mother covalent organic framework, and then the corresponding structural unit is used to perform an exchange reaction to prepare an irreversible amide-linked covalent organic framework material. The disclosure solves the problem of preparation of high-stability irreversible covalent organic framework, the introduced amide bond gives the covalent organic framework the ability to quickly and selectively recover precious metal gold from an aqueous solution, and the covalent organic framework can be used repeatedly.

Abstract: The disclosure belongs to the technical field of material science, and particularly relates to a method for preparing a high sensitivity temperature sensitive reversible color-changing microcapsule. According to the method, a series of novel fluorane dyes are designed and synthesized, the color-changing performance can be achieved without a color developing agent, the fluorane dyes are compounded with a phase change material according to a certain proportion, a series of two-component reversible temperature sensitive color-changing dyes are prepared, and then a two-component reversible thermochromic microcapsule with good color performance and color-changing response performance is prepared by adopting a solvent evaporation method. The two-component reversible thermochromic microcapsule provided by the disclosure can effectively alleviate the color lag phenomenon of a traditional three-component thermochromic capsule.

Abstract: The disclosure discloses a milk protein concentrate-based microencapsulation wall material and microcapsules, and belongs to the technical field of microcapsule preparation. In the disclosure, liquid or solid microcapsules are prepared by performing cation exchange treatment on milk protein concentrates, and then using the treated milk protein concentrates as a wall material. The prepared microcapsules have good physical and chemical stability during storage, and are suitable for protecting active functional factors and to be applied in the fields of food, medicine, health care products, cosmetics, daily chemicals and the like.

Abstract: The present disclosure discloses a casein micelle with composition and structural features simulating human milk and a preparation method thereof. The method includes the following steps: adding a citrate ion, a calcium ion, and a phosphate ion to a solution of compound casein to obtain a mixed solution; and then adjusting the pH of the mixed solution to 6.7-7.4, and achieving a constant volume with water to obtain a solution of the casein micelle simulating human milk. Wherein ?-casein, ?-casein, and ?s1-casein have a mass proportion of 65-71%, 17-23%, and 9-15% in the compound casein respectively. In the solution of the casein micelle simulating human milk, the concentrations of compound casein, citrate ion, calcium ion and phosphate ion were 0.3-0.6%, 1.5-4.2 mM, 4.5-10.8 mM, and 1.1-4.5 mM, respectively. The casein micelle simulating human milk of the present disclosure is similar to human milk casein micelle, and has low allergenicity.

Abstract: Disclosed is food-grade lubricating grease and a method for preparing the same, belonging to the technical field of lubricating grease. The food-grade lubricating grease is prepared from the following components in percentage by mass: 75% to 85% of food-grade white oil, 6% to 16% of stearic acid, 2.0% to 3.0% of benzoic acid, 4.7% to 8.7% of aluminum isopropoxide, 1.0% to 1.5% of water and 1.0% to 7.0% of nano-PTFE, and has good extreme-pressure, abrasion-resistant and friction-reduction properties, a last non-seizure load (PB) reaching 411.6 N, a sintering load (PD) reaching 1,960 N, and a friction coefficient reduced by 18.5%. The lubricating grease can be used for a food production industry and in household food appliances, the service life of a device and the service life of the food-grade lubricating grease are effectively prolonged, and meanwhile, food security is guaranteed to a certain degree.

Abstract: The disclosure relates to an enzymatic preparation method of inclusion complexes of tributyrin, and belongs to the technical field of oil microencapsulation. The disclosure combines enzymatic synthesis of cyclodextrin and inclusion of tributyrin with cyclodextrin, including enzymatic preparation of cyclodextrin with a CGT enzyme. Tributyrin is added in the preparation process; after reaction, Tween is added, and homogenization and spray drying are carried out. The effect of the finally obtained tributyrin powder is much better than that of single inclusion of tributyrin with cyclodextrin. The disclosure is simple in process, low in cost and convenient in operation; reaction processes are free of toxicity and pollution; there are no toxic reagent residues; the inclusion effect is obvious, and better utilization of a nutritional additive tributyrin in actual production is facilitated.

Abstract: The present disclosure discloses a preparation method of ginseng composition with high ginsenoside bioavailability. The composition is composed of ginseng powder of 250 meshes or more and ?-cyclodextrin, and the mass ratio of the ginseng powder to the ?-cyclodextrin is 1:0.05. Results show that when ?-cyclodextrin is used as a solid dispersant and added to the 1000-mesh ginseng powder at a mass ratio of 5%, the total dissolution amount is maximum; and furthermore, animal experiments (SD rats) show that the area under the plasma drug concentration-time curve (AUC0-48 h) after 48 h of ginseng powder ingestion is maximum, indicating that in-vivo bioavailability of the ginseng powder is the highest. The method of this application can effectively inhibit the aggregation of the ultra-finely pulverized 1000-mesh ginseng powder by adding the ?-cyclodextrin, thus significantly improving the total dissolution amount of ginsenoside.

Abstract: The disclosure discloses a production process and application of fermented tapioca starch for baking, and belongs to the fields of starch deep processing and food processing and production. The disclosure develops a production method of the fermented tapioca starch for baking. The method includes simple steps and greatly shortens a process cycle. By using tapioca starch as a main raw material and adding a specific amount of carbon source and a specific strain, under the action of fermentation and illumination in cooperation, the structure of the starch is improved. By adding the fermented tapioca starch, the effects of increasing the size of gluten-free Mochi bread, increasing pores of the bread and improving the texture and taste of the bread are realized.

Abstract: A processing system and processing method for blocked microreactor. The processing system comprises an gas intake device, a flushing device, a microreactor to be processed and a plasma processing device. One end of the microreactor to be processed is connected with the gas intake device and the flushing device through a pipeline; the other end of the microreactor to be processed is connected with a waste liquid bottle through the pipeline; and the microreactor to be processed is arranged between electrodes of the plasma processing device. The present invention uses the effective reactivity of plasma and active free radicals in an excitation atmosphere to crack micro blockage in a micro channel in a short time. The method of the present invention has high flexibility and strong controllability, and can select plasma electrodes according to blocked regions to crack the blockage in a specific region.