Abstract: The present disclosure discloses an ABO3 type high-entropy perovskite Bax(FeCoNiZrY)0.2O3-? electrocatalytic material and a preparation method thereof, belonging to the technical field of electrocatalytic materials. The electrocatalytic material is prepared by taking hydrated cobalt nitrate, hydrated ferric nitrate, hydrated nickel nitrate, barium nitrate, hydrated yttrium nitrate, hydrated zirconium nitrate and polyacrylonitrile staple fibers as raw materials through processes of liquid phase chelation, gelation, calcination, etc. The prepared high-entropy perovskite Bax(FeCoNiZrY)0.2O3-? electrocatalytic material can release more electrochemical active sites due to its special nanostructure, thus showing better electrocatalytic activity.

Abstract: The disclosure provides an active peptide with an anti-lipid oxidation function and a preparation method and application thereof and belongs to the technical field of plant-derived biologically active peptides. In the disclosure, oil processing by-products, namely oil crops after oil extraction, are used as the raw materials, and the raw materials are subjected to the steps of protein extraction, infrared pretreatment, proteolysis, freeze-drying, lipophilic part extraction, vacuum concentration and drying to prepare an anti-lipid oxidation peptide having the functional characteristics of scavenging DPPH free radicals, chelating metal ions, inhibiting lipid peroxidation, prolonging vegetable oil oxidation induction time, improving emulsion stability and the like.

Abstract: The disclosure discloses preparation of an L-amino acid deaminase mutant and application thereof, belonging to the technical field of gene engineering. Through pmirLAAD protein modification, analysis of a flexible loop structure around a binding site of the pmirLAAD product, and design of the best mutant, the modification method of the disclosure overcomes the defect that the catalytic efficiency of the previous wild-type enzyme is reduced due to product inhibition, and is tested by experiments. Compared with the control, the catalytic efficiency (1.61 mM?1·min?1) and the product inhibition constant (5.4 mM) of the finally obtained best mutant pmirLAADM4 are respectively increased by 5.2 times and 5.7 times. The yield of ?-ketoisovaleric acid can reach 96.5 g/L, and the transformation rate is greater than 97%. By adopting the method of the disclosure, the cost can be greatly reduced, and the industrialization process of production of ?-ketoisovaleric acid by an enzymatic transformation method is accelerated.

Abstract: Disclosed is a method for preparing high-load oral paclitaxel capsule for a slow release in colon, belonging to the field of porous starch drug loading. The preparation method of the present disclosure includes the following steps: (1) dripping an ethanol solution of paclitaxel into a water phase and drying the solution to obtain an amorphous paclitaxel microsphere; (2) redissolving the paclitaxel microsphere prepared in step (1) in the ethanol solution, dispersing porous starch in the ethanol solution for adsorption, volatilizing a solvent in an oven, washing the porous starch with the ethanol solution to remove unadsorbed paclitaxel, and centrifuging same to obtain a precipitate, namely the porous starch loaded with paclitaxel; and (3) dispersing the porous starch loaded with paclitaxel prepared in step (2) in a chitosan solution, dropwise adding the solution into a phytic acid solution, and stirring the solution for 4 hours to obtain a coated capsule.

Abstract: Disclosed is a method for promoting extracellular expression of proteins in B. subtilis using cutinase, which belongs to the technical fields of genetic engineering, enzyme engineering and microbial engineering. It teaches co-expressing a cutinase mutant and a target protein in B. subtilis to promote extracellular expression of the target protein which is naturally located inside cells. The target protein includes xylose isomerase, 4,6-?-glucosyltransferase, 4-?-glucosyltransferase, trehalose synthase, branching enzyme and the like. The invention can achieve extracellular expression of intracellularly localized target protein, improve the production efficiency, reduce the production cost and simplify the subsequent extraction process.

Abstract: The invention provides a motion capture method of a robotic arm, including: fastening a visual sensor on a robotic arm to acquire data as a source domain, fastening an inertial sensor on a corresponding human arm to acquire data as a target domain, and establishing a state space expression of a system; setting an optimal unknown state observed joint distribution by using a total probability theory and an observed prediction distribution of the source domain as a condition, decomposing a conditional joint observed distribution model, and solving an optimal distribution using KL divergence; and transferring knowledge of the source domain measured by the visual sensor into the target domain measured by the inertial sensor based on a Kalman filter and the total probability theory, performing data fusion based on Kalman filtering, and predicting a state of the system at a next moment to implement motion capture of the robotic arm.

Abstract: The disclosure discloses an L-aspartate ?-decarboxylase mutant and application thereof, and belongs to the technical field of enzyme engineering. In the disclosure, lysine at position 221 of L-aspartate ?-decarboxylase is mutated to arginine, glycine at position 369 is mutated to alanine, and the obtained new mutant enzymes have better temperature tolerance and are beneficial to industrial production. The K221R and G369A recombinant strains are subjected to high-density fermentation, and with sodium L-aspartate as a substrate, a whole cell catalytic reaction is carried out to prepare ?-alanine. Compared with a chemical production method, the method has the advantages that the production process is safe and clean, and has no environmental pollution. Compared with a pure enzyme catalysis method, the method has the advantages that the operation is simple and convenient. The yield of the final product ?-alanine reaches 91% and 90% respectively, and the concentration reaches 162.15 g/L and 160.42 g/L respectively.

Abstract: A method of preparing an enhanced yellow-emitting carbon dots includes dissolving citric acid and urea in N,N-dimethylformamide, reacting at a temperature of 150-180° C. for 4-8 h to obtain a carbonized mixture, separating and purifying the carbonized mixture through column chromatography using an eluent prepared according to a volume ratio of dichloromethane to methanol from high to low, collecting a solution obtained by elution when the volume ratio of dichloromethane to methanol is 1:1-2, performing rotary evaporation to remove the solvent, and drying to obtain the yellow-emitting carbon dot. The yellow-emitting carbon dot with fluorescence intensity enhanced with the increase of temperature is synthesized. The yellow-emitting carbon dots has application value in the fields of temperature detection, biological imaging, photoelectric equipment and the like.

Abstract: The invention provides a nanomaterial of polyglycerol grafted cellulose nanocrystal dendrimer and a preparation method thereof. In the present invention, the cotton linter pulp is pulverized into flocculent fibers, fed to sulfuric acid, and reacted. After centrifugation, dialysis and ultrasonic cell disruption, cellulose nanocrystals are obtained. Carboxylated cellulose nanocrystals are further obtained after oxidation with TEMPO, dialysis, and freeze-drying. The carboxylated nanocrystals are further added to DMF, and under a nitrogen atmosphere, glycidol is grafted to the hydroxyl groups on the surface by heating with stirring. After the reaction is completed, the product is centrifuged, dried under vacuum, dialyzed, and freeze dried to obtain a nanomaterial of primary polyglycerol grafted cellulose nanocrystals.

Abstract: The disclosure discloses recombinant Bacillus subtilis for synthesizing e lacto-N-neotetraose yield. The recombinant Bacillus subtilis is obtained by integrating two ?-1,4-galactotransferase genes on a genome of a host bacterium Bacillus subtilis 168?amyE:P43-lacY, P43-lgtB, PxylA-comK and exogenously expressing a ?-1,3-N-glucosaminotransferase gene. Compared with a strain before transformation, the recombinant Bacillus subtilis of the disclosure improves the yield of the synthesized lacto-N-neotetraose from 720 mg/L to 1300 mg/L, laying a foundation for further metabolic engineering transformation of Bacillus subtilis for producing the lacto-N-neotetraose.

Abstract: The disclosure discloses a method for quickly and accurately analyzing polyphenol content in rapeseed oil, and belongs to the field of analysis of natural compounds. The separation method of the disclosure uses acetonitrile-water as an extractant to extract polyphenols from the rapeseed oil, and cooperates with a C18 adsorbent for purification, and then performs separation and purification. Compared with the traditional liquid-liquid extraction and solid-phase extraction, the method has an average recovery rate of polyphenols in the rapeseed oil of 81.31% to 102.95%, and RSDs of 0.86% to 8.03%, and has higher accuracy and precision. The method of the disclosure not only uses less organic reagents and causes less environmental pollution, but also reduces matrix interference and improves purification efficiency through optimization of the adsorbent.

Abstract: Disclosed is a method for detecting aflatoxin B1 based on fluorescent copper nanoparticles, belonging to the technical fields of analytical chemistry, materials science and nano biosensing. In the disclosure, ?-CD@DNA-Cu NMs are prepared by using Y-shaped DNA as a template, ascorbic acid as a reducing agent and ?-CD as a fluorescence stabilizing and enhancing agent. Then, a ratiometric fluorescent probe is constructed based on the ?-CD@DNA-Cu NMs. Finally, the detection of AFB1 with high sensitivity, high selectivity and high accuracy is achieved by using the fluorescent probe. According to the method of the disclosure, in linear ranges of 0.03-10 ppb and 10-18 ppb, a ratio value of I433 nm/I650 nm and a concentration of AFB1 exhibit a good linear relationship respectively, and a limit of detection is 0.012 ppb (S/N=3). Metal ions Ca2+ may be replaced with Yb3+, Y3+, Er3+ and Pt2+, which are also suitable for increasing sensitivity of AFB1 in rice.

Abstract: Use of genomic NW_006882077.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 691045 in a CHO cell gene NW_006882077.1; a sequence of 5? NNNNNNNNNNNNNNNNNNNNNGG3? that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 690980-691090 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.

Abstract: A bionic oral cavity structure for testing and application thereof are provided for solving a technical problem that actual test process of the conventional bionic oral cavity structure needs to be improved. The bionic oral cavity structure for testing includes: an upper jaw, a lower jaw, a drive unit, a sensor and a controller, wherein a gum is made of a soft elastic material, and a base of the gum has a through-hole; a cap is formed by extending upward from a top opening of the through-hole; the tooth comprises a molar made of a hard material, and an implant; a top end of the implant passes through the through-hole of the gum and then extends into the cap; the gum and the implant are detachably mounted on the mounting plate; the sensor is a flexible sensor which covers the cap.

Abstract: The disclosure relates to the technical field of after-finishing of textile products, and in particular relates to a delayed-cure durable press finishing technology for cotton fabrics, including the following steps: singeing, desizing, liquid ammonia finishing, mercerizing, liquid ammonia finishing and post-cure finishing. The disclosure significantly improves the stability of a delayed-cure sensitized fabric during delayed-curing, relieves the problems caused by a slow reaction in the storage process of the delayed-cure sensitized fabric, and is favorable for improving the product quality.

Abstract: The disclosure discloses a bionic digestive tract as well as a preparation method and application thereof, belonging to the field of bionic technologies and the field of biological technologies. The bionic digestive tract of the disclosure is prepared by mixing a base material (one or more of silica gel, latex and hydrogel) and auxiliary materials (silicone oil and a curing agent) in a certain mass ratio (the mass ratio of the base material to the silicone oil to the curing agent is 100:(0.5 to 10):(0.5 to 3.5)). The simulation performance of the bionic digestive tract is excellent, has strong consistency with a true human digestive tract in terms of performance, structure and function, can simulate the true states of food, drugs and microorganisms in a digestive system, and has great application prospects in the research process of food and drugs.

Abstract: The disclosure discloses a temperature compensation circuit and method for a neural network computing-in-memory array. Reference arrays sparsely inserted in the computing-in-memory array are adopted to provide a reference voltage for ADCs, so that an input voltage and a reference voltage of the ADCs have a same temperature coefficient. Finally, after analog-to-digital conversion by the ADC, the digital output of the ADC is not affected by the external temperature, thereby ensuring the operational precision of the neural network. According to the temperature compensation circuit of the disclosure, the reference arrays have the same structure as the computing-in-memory array. The insertion density of the reference arrays is related to the temperature field where the computing-in-memory arrays are located.

Abstract: The present disclosure discloses a microwave processing method for food made of flour and rice fermented with sourdough and belongs to the technical field of food processing. It comprises: adding a sourdough to the raw material to prepare a steamed cake batter; fermenting the batter under the dielectric constant of 20˜34, the loss factor of 6.3˜9.0, the moisture content of 45˜55% and the water activity of 0.920˜0.980; and using microwave heating to obtain the microwave-steamed cake. Due to the regulating effect of the sourdough fermentation on the dielectric properties of the food material, it solves the problems of hard core, textual firmness and toughness, dryness and weak flavor caused by fast and uneven microwave heating without adding food additives; meanwhile, the sourdough fermentation process is optimized and the DY and additive amount of the sourdough are controlled to obtain the microwave-steamed cake with larger specific volume, lower hardness, more fine and uniform pores and better taste.

Abstract: The present invention provides a pyruvate-responsive biosensor and a construction method and use thereof. In the present invention, pyruvate-responsive biosensors with different dynamic ranges are successfully constructed by optimizing the PdhR binding sequence inserted on the P43 promoter and optimizing the insertion site, wherein the minimum increase in dynamic range is by 0.6 time, and the maximum increase is by 30.7 times. The pyruvate-responsive biosensors are useful in the precise control of the expression of each gene in the cell. Since pyruvate is a key metabolite of central carbon in the cells, these biosensors are capable of dynamically regulating the expression level of intracellular genes according to changes in the content of pyruvate in the cells, thereby achieving the dynamic control of intracellular metabolic flux. The pyruvate biosensor obtained in the present invention has a good specificity, and a response range to pyruvate of 10-35 nmol/g DCW.

Abstract: The present invention provides an edible blended vegetable oil for reducing blood lipids and cholesterol. The trace elements in the edible blended vegetable oil include 15-300 mg/kg of polyphenols, 290-1700 mg/kg of ?-sitosterol, 260-1500 mg/kg of campesterol, 150-1000 mg/kg of stigmasterol, 140-600 mg/kg of squalene, 50-160 mg/kg of parkerol, 40-120 mg/kg of ?-tocotrienol. The prepared edible blended vegetable oil of the present invention can achieve the effect of reducing blood lipids and cholesterol through a synergistic effect within the trace elements and a reasonable ratio within fatty acids. It is suitable for people with different health needs and has a broad market prospect and application value.