Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode, optionally included in a cover, configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species.
Type:
Grant
Filed:
October 27, 2016
Date of Patent:
October 13, 2020
Assignee:
Berkeley Lights, Inc.
Inventors:
Randall D. Lowe, Jr., Shao Ning Pei, Jian Gong, Alexander J. Mastroianni, Jason M. McEwen, Justin K. Valley
Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.
Type:
Grant
Filed:
September 6, 2018
Date of Patent:
October 13, 2020
Assignee:
Berkeley Lights, Inc.
Inventors:
Keith J. Breinlinger, Daniele Malleo, Gaetan L. Mathieu, J. Tanner Nevill, Alexander H. Slocum, Mark P. White
Abstract: A microfluidic device can include a base an outer surface of which forms one or more enclosures for containing a fluidic medium. The base can include an array of individually controllable transistor structures each of which can comprise both a lateral transistor and a vertical transistor. The transistor structures can be light activated, and the lateral and vertical transistors can thus be photo transistors. Each transistor structure can be activated to create a temporary electrical connection from a region of the outer surface of the base (and thus fluidic medium in the enclosure) to a common electrical conductor. The temporary electrical connection can induce a localized electrokinetic force generally at the region, which can be sufficiently strong to move a nearby micro-object in the enclosure.
Abstract: A zoned optical cup which mixes multiple channels of light to form a blended output, the device having discreet zones or channels including a plurality of reflective cavities each having a domed light converting appliance (DLCA) covering a cluster of LEDs providing a channel of light which is reflected upward by the cavities and mixed by angles walls and structures above the open top of the cavities in the common body of the cup.
Type:
Grant
Filed:
February 3, 2020
Date of Patent:
September 29, 2020
Assignee:
Ecosense Lighting Inc.
Inventors:
Raghuram L.V. Petluri, Paul Kenneth Pickard
Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.
Type:
Application
Filed:
January 15, 2020
Publication date:
September 24, 2020
Applicant:
Berkeley Lights, Inc.
Inventors:
Peter J. Beemiller, Alexander J. Mastroianni, Shao Ning Pei, Randall D. Lowe, JR., Annamaria Mocciaro, Kevin D. Loutherback, Yelena Bronevetsky, Guido K. Stadler, Andrew W. McFarland, Kevin T. Chapman, Duane Smith, Natalie C. Marks, Amanda L. Goodsell
Abstract: Systems and methods to provide multiple channels of light to form a blended white light output, the systems and methods utilizing recipient luminophoric mediums to alter light provided by light emitting diodes. The predetermined blends of luminescent materials within the luminophoric mediums provide predetermined spectral power distributions in the white light output.
Type:
Application
Filed:
June 8, 2020
Publication date:
September 24, 2020
Applicant:
Ecosense Lighting Inc.
Inventors:
Raghuram L.V. Petluri, Paul Kenneth Pickard
Abstract: Systems and methods to provide multiple channels of light to form a blended white light output, the systems and methods utilizing recipient luminophoric mediums to alter light provided by light emitting diodes. The predetermined blends of luminescent materials within the luminophoric mediums provide predetermined spectral power distributions in the white light output.
Type:
Grant
Filed:
July 30, 2018
Date of Patent:
September 15, 2020
Assignee:
EcoSense Lighting, Inc.
Inventors:
Raghuram L. V Petluri, Paul Kenneth Pickard
Abstract: The present disclosure provides methods for generating tunable white light. The methods include using a plurality of LED strings to generate light with color points that fall within white, red, and cyan color ranges, with each LED string being driven with a separately controllable drive current in order to tune the generated light output.
Type:
Grant
Filed:
January 17, 2020
Date of Patent:
September 15, 2020
Assignee:
Ecosense Lighting Inc.
Inventors:
Raghuram L. V. Petluri, Paul Kenneth Pickard
Abstract: The present disclosure provides methods for generating tunable white light with controllable circadian energy performance. The methods use a plurality of LED to generate light with color points that fall within blue, yellow/green, red, and cyan color ranges, with each LED being driven with a separately controllable drive current in order to tune the generated light output. Different light emitting modes can be selected that utilize different combinations of the plurality of LED in order to tune the generated white light.
Type:
Application
Filed:
May 20, 2020
Publication date:
September 10, 2020
Applicant:
Ecosense Lighting Inc.
Inventors:
Raghuram L.V. Petluri, Paul Kenneth Pickard
Abstract: A lighting device assembly includes: a heat sink; a light source attached to one end of the heat sink; an optic assembly configured to pivot an optic about the light source; a housing member having a cavity in which at least a portion of the optic assembly is received; and an elastic member configured to press the optic assembly against the cavity to maintain an adjusted position of the optic.
Abstract: Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.
Type:
Grant
Filed:
April 22, 2016
Date of Patent:
August 25, 2020
Assignee:
Berkeley Lights, Inc.
Inventors:
Xiao Guan, Mark P. White, Jason M. McEwen, Gang F. Wang, Kevin T. Chapman, Xiaohua Wang, Christine E. Sun