Abstract: The present invention relates to activatable promoters, as well as expression systems, vectors, delivery systems, reaction vessels, aqueous objects and compositions comprising said activatable promoters. The invention also provides compositions comprising assemblies and networks of aqueous objects comprising said activatable promoters. The invention additionally provides methods of expressing a transcript and methods of expressing a polypeptide using the activatable promoters of the invention. The invention further provides phospholipid mixtures, as well as aqueous objects and compositions comprising said phospholipid mixtures.

Abstract: Immunogenic compositions and methods of their use in eliciting immune responses to coronaviruses, such as SARS-CoV-2 are provided.

Abstract: The present invention provides recombinant adenoviral vectors, immunogenic compositions thereof and their uses in medicine. In particular, the present invention provides an adenoviral vector comprising the genome of an adenovirus other than AdHu5 and AdY25, wherein the genome of the adenovirus has been modified such that the vector lacks the native E4 locus of the adenovirus and comprises heterologous E4Orf1, E4Orf2 and E4Orf3 coding regions from AdY25.

Abstract: The present application relates to an electrode suitable for use in electrochemical sensing of a target species. The electrode comprises a peptide monolayer of defined length and to one end of which are attached both a redox active species and a receptor that is capable of binding to the target species. Also provided is an electrochemical method of sensing a target species, which involves the use of the electrode.

Abstract: The invention relates to a method for sequencing a heteropolymeric target nucleic acid sequence that involves stochastic sensing. The invention also relates to a method for improving a pore for sequencing a target nucleic acid sequence by modifying one or more sites in the pore.

Abstract: The invention relates the use of single particle light scattering, preferably interferometric scattering microscopy (also referred to herein as iSCAT), to measure the concentration of a particle in a solution. The invention furthermore relates to the use of light scattering to detect lipoprotein particles in a sample, and to related diagnostic and treatment methods.

Abstract: The present invention relates to peptides, in particular cell-penetrating peptides, and to conjugates of such cell-penetrating peptides with a therapeutic molecule. The present invention further relates to use of such peptides or conjugates in methods of treatment or as a medicament, especially in the treatment of genetic disorders and in particular muscular dystrophies such as Duchenne muscular dystrophy.

Abstract: The invention relates to an adjuvant comprising Pattern Recognition Receptor (PRR) agonist molecules linked to polymer chains that are capable of undergoing particle formation in aqueous conditions, or in aqueous conditions in response to external stimuli; and methods of treatment or prevention of disease using such an adjuvant.

Abstract: The invention relates to a composition for inducing a T cell mediated immune response for the treatment or prevention of prostate cancer comprising a modified Vaccinia virus Ankara (MVA) vector expressing the 5T4 antigen polypeptide under control of a poxvirus F11 promoter. Suitably said poxvirus F11 promoter is the endogenous MVA F11 promoter. More suitably said vector expresses a polypeptide having the amino acid sequence of SEQ ID NO: 1 or said vector expresses a polypeptide encoded by a polynucleotide having the nucleic acid sequence of SEQ. ID NO: 2. The invention also relates to uses and methods.

Abstract: An interferometric scattering microscope is adapted by performing spatial filtering of output light, which comprises both light scattered from a sample location and illuminating light reflected from the sample location, prior to detection of the output light. The spatial filtering passes the reflected illumination light but with a reduction in intensity that is greater within a predetermined numerical aperture than at larger numerical apertures. This enhances the imaging contrast for coherent illumination, particularly for objects that are weak scatterers.

Abstract: The invention relates to a method for sequencing a heteropolymeric target nucleic acid sequence that involves stochastic sensing. The invention also relates to a method for improving a pore for sequencing a target nucleic acid sequence by modifying one or more sites in the pore.

Abstract: The present invention provides a combined therapy comprising the use of one or more omega-3 fatty acids and one or more B vitamins and for treating cognitive impairment, such cognitive impairment disorders include, especially but not exclusively, Mild Cognitive Impairment (MCI) and Alzheimer's disease (AD). The present invention also provides a treatment for individuals suffering from cognitive impairment disorders that occur as a result of brain or cerebral atrophy, the invention includes inter alia methods of treating and/or reducing progression of brain atrophy and pharmaceutical compositions and nutritional supplements therefor.

Abstract: The invention relates to a process for producing a compound comprising an 18F-difluoromethyl group or a compound comprising an 18F-difluoromethylene group, which process comprises contacting a compound comprising a nucleophilic group with 18F-difluorocarbene. The invention also relates to a compound of Formula (I). Compounds comprising an 18F-difluoromethyl group or an 18F-difluoromethylene group are also the subject of the present invention.

Abstract: The application discloses a method and apparatus for imaging a sample by interferometric scattering microscopy, the method comprising illuminating a sample with at least one coherent light source, the sample being held at a sample location comprising an interface having a refractive index change, illuminating the sample with illuminating radiation to generate a backpropagating signal from the sample comprising light reflected at the interface and light scattered by the sample, splitting the backpropagating signal into first and second signals, modifying the second signal using a modifying element such that the second signal differs from the first signal, directing the first and second signals onto first and second detectors to generate, respectively, first and second images and comparing, by a processor, the first and second images to determine one or more characteristics of the sample.

Abstract: Disclosed are conjugates of an oligonucleotide and a peptide covalently bonded or linked via a linker to the oligonucleotide, the peptide including at least one cationic domain comprising at least 4 amino acid residues and at least one hydrophobic domain comprising at least 3 amino acid residues, provided that the peptide includes a total of 7 to 40 amino acid residues and does not include any artificial amino acid residues; and the oligonucleotide including a total of 12 to 40 contiguous nucleobases, where at least 12 contiguous nucleobases are complementary to a target sequence in a human dystrophin gene.

Abstract: The present invention provides recombinant adenoviral vectors, immunogenic compositions thereof and their uses in medicine. In particular, the present invention provides an adenoviral vector comprising the genome of an adenovirus other than AdHu5 and AdY25, wherein the genome of the adenovirus has been modified such that the vector lacks the native E4 locus of the adenovirus and comprises heterologous E4Orf1, E4Orf2 and E4Orf3 coding regions from AdY25.

Abstract: This disclosure teaches multi-classification of endoscopic imaging using a segmentation neural network. The network is trained on imaging data using a novel loss function with components of both focal and boundary loss. During a lithotripsy procedure, images are received by a deployed endoscopic probe. Based on the received imaging data and inference data from its prior learning, the segmentation neural network identifies renal calculi and surgical instruments within the imaging data. The imaging data is modified and displayed to assist the lithotripsy procedure.

Abstract: A process for preparing a fluorinating reagent from a calcium-containing compound is disclosed. The process bypasses the requirement to form hydrofluoric acid. The fluorinating reagent can be used to prepare high-value fluorochemicals.

Abstract: The invention relates to a process for moving a droplet from a first location to a second location, which droplet comprises a magnetic material disposed in an aqueous medium. The invention also relates to a droplet assembly comprising a droplet, which droplet comprises a magnetic material disposed in an aqueous medium. Various uses of the droplet assembly are also described.

Abstract: This disclosure teaches determining a renal calculus size based on calibration data generated from an endoscopic imager. During a lithotripsy procedure, images are received by a deployed endoscopic probe. Based on the received imaging data, the generated calibration data, and known properties of the endoscopic probe, one or more renal calculi in the images are identified and sized. The determined size values are displayed with the images of the renal calculi.