Abstract: The present disclosure relates to materials and methods for spatial detection of nucleic acid in a tissue sample or a portion thereof. In particular, provided herein are materials and methods for detecting RNA so as to obtain spatial information about the localization, distribution or expression of genes in a tissue sample. In some embodiments, the materials and methods provided herein enable detection of gene expression in a single cell.

Abstract: A device for detecting postpartum hemorrhage is provided. A mat defines a proximal end and a distal end. The mat is configured to be disposed between a support and a patient and has a surface with a fluid flow region configured to direct flow of bodily fluids from the patient towards the distal end of the mat. A first detachable and optionally sealable bag is configured to receive the bodily fluids from the patient in a first operational mode of the device. A second sealable bag is configured to receive the bodily fluids from the patient in a second operational mode of the device. The second sealable bag comprises a visual volume indicator to measure the bodily fluids collected in the second sealable bag.

Abstract: An organic optoelectronic device comprises a substrate having first and second regions, a first electrode positioned over the first region of the substrate, a shutter electrode positioned over the second region of the substrate, an organic heterojunction layer comprising an organic heterojunction material, positioned over at least a portion of the first electrode, an insulator layer positioned over at least a portion of the shutter electrode, an organic channel layer, comprising an organic channel material, positioned over at least a portion of the heterojunction and insulator layers, and a second electrode positioned over the channel layer in the second region of the substrate, wherein the shutter electrode is configured to generate a repulsive potential barrier in the channel layer, suitable to at least reduce movement of charge in the channel layer. A method of measuring received light in an optoelectronic device is also described.

Abstract: The present disclosure relates to methods for characterizing and diagnosing dental diseases. In particular, the present disclosure relates to methods for characterizing and diagnosing dental disease (e.g., gingivitis and periodontal disease) based on levels of AI-2 in oral fluids and plaque.

Abstract: A method of fabricating a thin film structure includes printing, using an electrohydrodynamic jet (e-jet) printing apparatus, a first layer comprising a first liquid ink, such that the first layer is supported by a substrate, curing the first layer; printing, using the e-jet printing apparatus, a second layer comprising a second liquid ink, such that the second layer is supported by the first layer, and curing the second layer.

Abstract: Methods of preparing engineered cementitious composite precursors include carbonating a fly ash comprising >about 25% by weight of calcium oxide (CaO) and having a water content of >about 12% to <about 18% by weight of water by exposing the fly ash to a first gas stream comprising carbon dioxide to form a carbonated fly ash. A steel slag is also carbonated that comprises >about 40% by weight of calcium oxide (CaO) and having a water content of >about 12% to <about 18% by weight of water by exposing the steel slag to a second gas stream comprising carbon dioxide to form a carbonated steel slag. The carbonated fly ash and the carbonated steel slag are suitable for use as engineered cementitious composite precursors in a bendable engineered cementitious composite composition that further comprises Portland cement, a polymeric fiber, and a superplasticizer.

Abstract: Provided herein are systems, methods, and articles of manufacture for collecting and merging two different size droplets using a substrate comprising a plurality of trapping sites. In certain embodiments, provided herein are systems composed of a plurality of larger droplets and smaller droplets and a substrate comprising a plurality of trapping sites where each trapping site is configured to trap only one of the larger droplets and only one of the smaller droplets when the larger droplet is already present at the trapping site. In particular embodiments, the larger and/or smaller droplets are sorted prior to being contacted with the substrate to ensure they contain the desired component (e.g., cell or barcoded bead). In other embodiments, each trapping site is composed of one or multiple fluidically linked capture wells. In some embodiments, collected larger and smaller droplets are merged (e.g., via a demulsifier or electricity).

Abstract: The present invention provides methods, compositions, systems, and kits comprising nano-satellite complexes and/or serum albumin carrier complexes, which are used for modulating antigen-specific immune response (e.g., enhancing anti-tumor immunity). In certain embodiments, the nano-satellite complexes comprise: a) a core nanoparticle complex comprising a biocompatible coating surrounding a nanoparticle core; b) at least one satellite particle attached to, or absorbed to, the biocompatible coating; and c) an antigenic component conjugated to, or absorbed to, the at least one satellite particle component. In certain embodiments, the complexes further comprise: d) an type I interferon agonist agent. In some embodiments, the serum albumin complexes comprise: a) at least part of a serum albumin protein, b) an antigenic component conjugated to the carrier protein, and c) a type I interferon agonist agent.

Abstract: The disclosure provides materials in the form of flavivirus variants that each encode a Non-Structural Protein-1 (NS1) variant, wherein the coding region is a chimera of at least two different NS1 coding regions, or wherein the coding region has at least one mutation in a codon of a canonical Asn-Xxx-Ser/Thr N-linked glycosylation site, wherein Asn is asparagine, Xxx is any amino acid, and Ser/Thr is either serine or threonine, or wherein the coding region is both a chimera and has at least one mutation in a codon of a canonical N-liked glycosylation site, wherein Asn is asparagine, Xxx is any amino acid, and Ser/Thr is either serine or threonine. The disclosure also provides methods of using such flavivirus variants to inhibit the transmission of infectious flavivirus.

Abstract: Methods for performing non-invasive thrombolysis with ultrasound using, in some embodiments, one or more ultrasound transducers to focus or place a high intensity ultrasound beam onto a blood clot (thrombus) or other vascular inclusion or occlusion (e.g., clot in the dialysis graft, deep vein thrombosis, superficial vein thrombosis, arterial embolus, bypass graft thrombosis or embolization, pulmonary embolus) which would be ablated (eroded, mechanically fractionated, liquefied, or dissolved) by ultrasound energy. The process can employ one or more mechanisms, such as of cavitational, sonochemical, mechanical fractionation, or thermal processes depending on the acoustic parameters selected. This general process, including the examples of application set forth herein, is henceforth referred to as “Thrombolysis.

Abstract: A roadmanship system comprises a computational device and a vehicle comprising a plurality of sensors and a vehicle control system in communication with the computational device and the plurality of sensors. The computational device can be configured to: (i) receive driving data from a group of vehicles; (ii) calculate a regression curve based on the driving data; (iii) calculate a threshold value of an engineering parameter based on the regression curve and a predetermined roadmanship level; and (iv) output the threshold value to the vehicle control system. The vehicle control system can be configured to: (a) receive the threshold value from the computational device; (b) receive operational information associated with at least one of the vehicle and a driving environment surrounding the vehicle from the plurality of sensors; and (c) cause the vehicle to perform a vehicle maneuver based on the threshold value and the operational information.

Abstract: Three-dimensional (3D) micro-scale shells are presented with openings of various sizes and geometries on the surface. The shell consist of a suspended ring-shaped resonator, multiple support beams, a support post, and a cap region that connects the support beams to the support post. Shells with openings of various sizes and geometries allow the creation of micro electromechanical systems (MEMS) sensors and actuators with a wide range of engineered mechanical and electrical properties. The openings on the shell surface can, for example, control the mechanical quality factor (Q) and resonance frequencies of the shell when the shell is used as a suspended proof mass of a mechanical resonator of a vibratory gyroscope. The shells can also serve as mechanical supporting layers and/or an electrode connection layer for MEMS actuators and sensors that use 3D shells as proof masses.

Abstract: A fully automated ultrasound apparatus includes a sensor or probe which can be initially manually attached to a side of the neck of a patient, an ultrasound interface to control the sensor and periodically acquire raw ultrasound data, a signal and image processing system to autonomously convert the raw ultrasound data into a measurement that is useful to physicians, and a display to relay the current measurements and measurement history to provide data trends. The sensor can include one or more ultrasound transducers built into a housing. A disposable component can serve to secure the sensor to the neck of the patient and to provide a coupling medium between the sensor and the skin of the patient.

Abstract: The present disclosure relates to methods of determining a treatment course of action. In particular, the present disclosure relates to mutations in the gene encoding estrogen receptor and their association with responsiveness to estrogen therapies for cancer.

Abstract: An electron cyclotron resonance (ECR) thruster includes a magnetic field source configured to generate a magnetic field, a thruster body that defines a chamber, the thruster body being disposed relative to the magnetic field source such that the magnetic field is present in the chamber and such that a magnetic nozzle is established, an antenna configured to propagate radio frequency (RF) power within the chamber, and a waveform generator coupled to the antenna to generate an RF waveform for the RF power. The waveform generator is configured such that the RF waveform includes multiple frequencies.

Abstract: Disclosed is compound of formula I and the pharmaceutically acceptable salts and solvates thereof, wherein R, R1a, R1b, R1h, L2, L3, #, #, # are as defined as set forth in the re) specification. Disclosed is compound of formula I for use to treat a condition or disorder responsive to Mcl-1 inhibition such as cancer.

Abstract: The disclosure provides biocatalysts that halogenate complex chemical compounds in specific and predictable ways. Also disclosed are halogenated complex organic compounds. The disclosure further provides methods for the halogenation of complex chemical compounds and methods of inhibiting the contraction of smooth muscle in mammals.

Abstract: A method of providing an augmented reality game environment within a game space includes obtaining, by a processor, sensor data for the game space, determining, by the processor, a position of a player in the game space based on the sensor data, generating, by the processor, player image data of a peripersonal boundary of the player based on the determined position of the player for rendering a representation of the peripersonal boundary in the game space, the peripersonal boundary being disposed about, and spaced from, the determined position, obtaining, by the processor, player data for the player via an input modality, the player data being indicative of a player directive to modulate the peripersonal boundary, adjusting, by the processor, a size of the peripersonal boundary as a function of the player data, and updating, by the processor, the player image data based on the adjusted size of the peripersonal boundary.

Abstract: A machine vision method includes obtaining a first representation of an image captured by an image sensor array, analyzing the first representation for an assessment of whether the first representation is sufficient to support execution of a machine vision task by the processor, if the first representation is not sufficient, determining, based on the first representation, a region of the image of interest for the execution of the machine vision task, reusing the image captured by the image sensor array to obtain a further representation of the image by directing the image sensor array to sample the image captured by the image sensor array in a manner guided by the determined region of the image of interest and by the assessment, and analyzing the further representation to assess whether the further representation is sufficient to support the execution of the machine vision task by implementing a procedure for the execution of the machine vision task in accordance with the further representation.

Abstract: Provided herein is technology relating to materials having microscale and/or nanoscale features and particularly, but not exclusively, to porous materials comprising microscale features, methods for producing porous materials comprising microscale features, drug delivery vehicles, and related kits, systems, and uses.