Abstract: Systems and methods for structuring unstructured data according to a data object structure that enables fast query look-ups across a variety of space and time dimensions. Furthermore, many embodiments optimize the storage of the data objects using a set of compression techniques that configure the data types used for the data objects based on properties of the stored data. Furthermore, many embodiments provide are able to service query look-up requests without having to deserialize data within the byte stream format as stored in memory by encoding information that provide memory locations for requested data, thereby allowing for the immediate retrieval of the data as it is stored in the persistent memory.

Abstract: Systems and method for light field imaging with mirror arrays in accordance with embodiments of the invention are illustrated. One embodiment includes a light field imaging system, including a mirror array where each mirror in the mirror array is positioned to reflect light from a target object towards a lens such that a different view of the target object is visible in each mirror from the viewpoint of the lens. The system further includes a lens configured to focus the reflected light, a sensor configured to capture an image depicting a plurality of reflections of the target object, a processor, and a memory containing a light field imaging application that directs the processor to obtain the captured image, extract a light field of the target object based on the plurality of reflections of the target object, and construct a three-dimensional model of the target object based on the light field.

Abstract: Various embodiments comprise systems, methods, architectures, mechanisms, apparatus, and improvements thereof for scaling and summing a plurality of weighted-data-representative analog signals provided by columns of in-memory computing bit cells within an N×M array of bit cells such that analog accumulation or summation of the weighted-data-representative analog signals provides a scaled result for further processing.

Abstract: An apparatus and method for extracting nitrogen including compounds from wastewater is disclosed herein. The methods and apparatus use molecularly designed selective electrocatalysts, electrolytes, and separation reactors to enable automated, distributed ammonia manufacturing with minimal environmental impacts. In some embodiments, a method of nitrogen reduction of wastewater is provided. The method includes: providing an apparatus including: a first chamber comprising a salt solution; a second chamber comprising a wastewater source; a third chamber comprising a salt solution and a third electrode, wherein the second chamber is positioned between the first chamber and the third chamber; an anion exchange membrane positioned between the first chamber and the second chamber; and a cation exchange membrane positioned between the second chamber and the third chamber; applying a potential bias between any two of the first chamber, the second chamber, and the third chamber.

Abstract: A non-reciprocal microwave network is provided that includes an in-line ferromagnetic element with adjoining polarizing adapters to achieve directivity via a multi-mode interaction at or near the ferrite to act as new class of 4-port circulator or 2-port isolator, with standard waveguide inputs for assembly in larger networks.

Abstract: The presently disclosed subject matter provides a biomimetic lung disease model, and methods of its production and use. In one exemplary embodiment, the biomimetic lung disease model can include a first and second microchannel with a membrane coated with airway epithelial cells disposed between the microchannels and at least one device coupled to the biomimetic model that delivers an agent to at least one microchannel. In certain embodiments, the agent is cigarette smoke.

Abstract: An apparatus and method for medical imaging uses a first and second contrast agent, the second agent targeted to a particular tissue type. First images are obtained using the first agent, and second images using the second agent using medical imaging systems. An image processing system is adapted to process the first and second medical images by fitting parameters of a pharmacokinetic model to the first medical images, identifying a nontargeted tissue type, scaling the fitted parameters to best match the nontargeted tissue in the second medical images, executing the pharmacokinetic model to prepare a correction image, and generating corrected medical images by subtracting the correction image from the second medical images.

Abstract: The invention provides a supramolecular polymer composition capable of co-assembly to maintain a three dimensional (3-D) macrostructural form after 3-D printing, made of a solvent, a template molecule; and a reactive component. The reactive component can be at least one monomer that is capable of hydrogen bonding with the template molecule to form a 1D supramolecular structure. The template may be an amphiphilic polymer. The monomer has at least two pendant groups capable of covalent crosslinking. The invention also includes a 3-D structure formed by crosslinking a 3-D printed supramolecular polymer composition, which optionally has a mesoporous structure. Also included is a method of manufacturing a 3-D structure by delivering a supramolecular polymer composition onto a surface of a substrate to form the 3-D structure.

Abstract: Disclosed are methods, systems, components, and compositions for synthesis of sequence defined polymers. The methods, systems, components, and compositions may be utilized for incorporating novel substrates that include non-standard amino acid monomers and non-amino acid monomers into sequence defined polymers. As disclosed herein, the novel substrates may be utilized for acylation of tRNA via flexizyme catalyzed reactions. The tRNAs thus acylated with the novel substrates may be utilized in synthesis platforms for incorporating the novel substrates into a sequence defined polymer.

Abstract: A component with a reflective substrate, a photoresist layer disposed on the reflective substrate, and a light diffusing layer sandwiched between the reflective substrate and the photoresist layer is provided. The light diffusing layer includes an outer metal oxide layer with an outer rough surface configured to diffuse laser light during laser interference lithography of the photoresist layer. The outer metal oxide is also configured to be reduced to a conductive metallic layer during electroplating of the substrate. The outer metal oxide layer includes a plurality of elongated light diffusing elements extending in an outward direction from the substrate such that the outer rough surface diffuses at least 90% of laser light during the laser interference lithography of the photoresist layer.

Abstract: A technique is presented for quantizing pixels of an image using Sigma Delta quantization. In one aspect, pixel values for an image are segmented into columns of pixel values; and for each column in the matrix, pixel values of a given column are quantized using sigma delta modulation. The pixel values in a given column are preferably quantized as a whole, thereby minimizing accumulated quantization error from a starting pixel value in the given column to a current pixel value in the given column. In another aspect, the pixels of an image are quantized using a 2D generalization of Sigma Delta modulation.

Abstract: De novo artificial protein based reporters that may be expressed in eukaryotic (e.g., mammalian) cells and methods of using the same are provided herein.

Abstract: The present disclosure relates to methods of detecting manganese in a solution using resacetophenone oxime by spectrophotometric analysis wherein the resacetophenone oxime produces colorimetric responses to indicate the presence of manganese. The resacetophenone oxime is not only stable in solution, but it also may be inserted into a hydrocolloid gel to facilitate a “spot test” for the detection of manganese, resulting in a long shelf life. These manganese testing methods disclosed herein may be used at the pre-disinfection process at water treatment facilities. The disclosed methods of manganese testing may be combined with an ammoniacal buffer reagent that may be used by water processing facilities, prior to final disinfection, to augment existing manganese (II) efforts of removal. A secondary flocculation with this buffer will scavenge additional manganese (II), that can then be removed by sedimentation or filtration.

Abstract: High affinity PD-1 mimic polypeptides are provided, which (i) comprise at least one amino acid change relative to a wild-type PD-1 protein; and (ii) have an increased affinity for PD-L1 relative to the wild-type protein. Compositions and methods are provided for modulating the activity of immune cells in a mammal by administering a therapeutic dose of a pharmaceutical composition comprising a high affinity PD-1 mimic polypeptide, which blocks the physiological binding interaction between PD-1 and its ligand PD-L1 and/or PD-L2.

Abstract: Disclosed is a biogel nanosensor for detection of an analyte that includes an acryloyl or methacryloyl modified hydrogel and nucleic acid amplification reagents in picoliter or nanoliter volume in the form of microarray. Also disclosed are methods of making the disclosed biogel nanosensor, and methods of using the biogel nanosensors.

Abstract: Here we show that P. ananatis produces at least three phosphonates, two of which were purified and structurally characterized. The first, designated pantaphos, was shown to be 2-(hydroxy(phosphono)methyl)maleate; the second, a probable biosynthetic precursor, was shown to be 2-(phosphonomethyl)maleate. Purified pantaphos is both necessary and sufficient for the hallmark lesions of onion center rot. Moreover, when tested against mustard seedlings, the phytotoxic activity of pantaphos was comparable to the widely used herbicides glyphosate and phosphinothricin. Pantaphos was also active against a variety of human cell lines.

Abstract: A method of producing para-hydroxybenzoic acid (pHBA) or a derivative thereof includes culturing the recombinant microorganism in a fermentation broth, wherein said recombinant microorganism comprising a genetically engineered pathway expressing at least one nucleic acid sequence encoding a polypeptide selected from: an exogenous chorismate pyruvate lyase of EC 5.4.4.2 or EC 4.1.3.40; an exogenous 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase of EC 4.1.2.15, or EC 2.5.1.54; an exogenous shikimate kinase of EC 2.7.1.71; or an exogenous 3-dehydroquinate dehydratase (DHQ) of EC 4.2.1.10; adding a carbon source to the fermentation broth; and isolating the pHBA from the fermentation broth.

Abstract: Disclosed is an enabling process for high throughput manufacturing of large variety of chemical and biological sensors that utilize well-developed spin-based sensing of analyte binding. A method for producing a nanometer scale analyte specific sensor may be provided, that includes providing a first material (such as a diamond) having a luminescent center (such as an NV center) within predetermined distance (such as 2-5 nm) of a first surface, where the luminescent center configured to emit within an optical emission range, providing photochemically active molecules (such as molecules with a photo-uncaging protective group) where an optical absorption range of the photochemically active molecules at least partially overlaps the optical emission range, coating the first surface of the first material with the photochemically active molecules, and may include placement of the photochemically active surface molecule within a distance smaller than the Forster Resonant Excitation Transfer (i.e.

Abstract: A method for treating a subject suffering from a disease is provided. The method includes diagnosing the subject as suffering from a disease; upregulating the cathelicidin gene CAMP in the subject; and administering to the subject at least one treatment for the disease.

Abstract: The present disclosure provides methods and compositions for treating X-CGD in subjects, comprising genetically modifying cells from the subjects ex vivo by integrating a functional, codon-optimized CYBB cDNA at the endogenous CYBB locus.