Abstract: Materials and methods for using modified Cas9-APOBEC fusion polypeptides for targeted modification of specific DNA sequences are provided herein.

Abstract: A method is provided for predicting that a patient will require a blood transfusion during a treatment. The method includes obtaining, on a processor, first data that indicates values for one or more parameters of a characteristic of a continuous photoplethysmographic (PPG) waveform collected during the treatment. The method further includes applying, on the processor, coefficients to the values for the one or more parameters. The method further includes determining, on the processor, second data that indicates a prediction that the patient will require the blood transfusion during the treatment based on applying the coefficients to the values for the one or more parameters. An apparatus is also provided for predicting that the patient will require the blood transfusion during the treatment.

Abstract: An additive manufacturing device utilizing an electron beam and laser integrated scanning comprises: a vacuum generating chamber (1); a worktable means having a forming region at least provided in the vacuum generating chamber (1); a powder supply means configured to supply a powder to the forming region; an electron-beam emission focusing and scanning means (6) and an laser-beam emission focusing and scanning means (7) configured in such a manner that a scanning range of the electron-beam emission focusing and scanning means (6) and a scanning range of the laser-beam emission focusing and scanning means (7) cover at least a part of the forming region; and a controller configured to control the electron-beam emission focusing and scanning means (6) and the laser-beam emission focusing and scanning means (7) to perform a powder integrated-scanning and forming treatment on the forming region.

Abstract: Provided herein are materials and in planta methods for using haploid inducer lines containing a targeted endonuclease to generate transgenic or non-transgenic plants with targeted mutations and/or genomic modifications.

Abstract: The present invention provides compositions and methods for transducing cells (e.g. T cells or immune cells). Also provided herein are methods of treating a disease in a subject in need thereof.

Abstract: The present invention relates to a recombinant microorganism having an enhanced ability to produce heme, coproporphyrin III (Copro III), and uroporphyrin III (Uro III), and a method for producing heme, coproporphyrin III, and uroporphyrin III using same. When using a recombinant microorganism incorporating a gene that codes glutamyl-tRNA reductase (HemA), glutamate-1-semialdehyde aminotransferase (HemL), and diphtheria toxin repressor (DtxR), which is a transcription factor capable of inducing the expression of genes related to heme metabolic pathways, porphyrin-based structures can be produced at high yield, and thus the method is economic.

Abstract: Disclosed is a kit for detecting content of fluoride ions in a microsample, including: at least one 96-well plate, reagent A, reagent B, reagent C, reagent D, reagent E and a fluoride standard solution having a concentration of 2.5 mg/L. The kit can be used to effectively overcome the uncertainties in the existing methods for detecting fluoride ions, and also involves rapid and convenient operation. Moreover, this method involves simple and rapid operation, the use of a small amount of a sample and simultaneous detection of multiple samples. This kit provides a more standardized detection to lower the human error, thereby allowing for a more reliable result and for a suitable application in the on-site detection of content of fluoride ions in various environments such as in water quality engineering or in the laboratory.

Abstract: An external transmitter apparatus for a transcutaneous energy transfer (TET) system for supplying power for use in energising an implantable medical device is disclosed, the apparatus comprising an external transmitter apparatus comprising a plurality of transmitter coils for delivering power transcutaneously to one of a plurality of receiver coils of an implantable receiver apparatus of the TET system when located in proximity thereto. The external transmitter apparatus is provided with power by a pulsed power supply. The coils of the external transmitter apparatus and the implantable receiver apparatus may be printed on flexible substrates. Also disclosed are methods of operating such a system, an external transmitter apparatus for use in such a system, an external transmitter apparatus and an implantable receiver apparatus including flexible coils.

Abstract: A ride attraction system includes a tower track and a ride vehicle configured to accommodate one or more riders. The ride vehicle is coupled to and configured to move relative to the tower track and the ride vehicle includes one or more user input devices. The ride attraction system further includes an image system configured to display a ride environment, wherein the user input devices are configured to enable the one or more riders to interact with elements of the ride environment via the one or more user input devices. The ride attraction system further includes a controller communicatively coupled to the ride vehicle and the image system and configured to control movement of the ride vehicle relative to the tower track based on signals from the one or more user input devices.

Abstract: The present invention provides amphiphilic telodendrimers that aggregate to form nanocarriers characterized by a hydrophobic core and a hydrophilic exterior. The nanocarrier core may include amphiphilic functionality such as cholic acid or cholic acid derivatives, and the exterior may include branched or linear poly(ethylene glycol) segments. Nanocarrier cargo such as hydrophobic drugs and other materials may be sequester in the core via non-covalent means or may be covalently bound to the telodendrimer building blocks. Telodendrimer structure may be tailored to alter loading properties, interactions with materials such as biological membranes, and other characteristics.

Abstract: A thermionic emission device comprises a first electrode, a second electrode, a single carbon nanotube, an insulating layer and a gate electrode. The gate electrode is located on a first surface of the insulating layer. The first electrode and the second electrode are located on a second surface of the insulating layer and spaced apart from each other. The carbon nanotube comprises a first end, a second end opposite to the first end, and a middle portion located between the first end and the second end. The first end of the carbon nanotube is electrically connected to the first electrode, and the second end of the carbon nanotube is electrically connected to the second electrode.

Abstract: The present invention provides an automatic jet breaking tool for solid fluidization exploitation of natural gas hydrate, which mainly includes an upper joint, an outer cylinder, an inner sliding sleeve, a lockup sliding sleeve, a thrust bearing, a spring, a jet joint, a telescopic jet sprinkler, a plug block and an extrusion seal ring. The present invention mainly adopts the principle of throttling control pressure to control the position of the inner sliding sleeve by controlling a flow rate of a drilling fluid, so as to turn on and turn off the jet breaking tool. The application of the present invention can realize automatic jet breaking of solid fluidization exploitation of the natural gas hydrate, reduce procedures of a round trip operation, and effectively improve the efficiency and safety of the exploitation operation of the natural gas hydrate.

Abstract: In an embodiment, the present disclosure pertains to a cavitation generation device that includes a dactyl plunger rotatable about an axis between an open position and a closed position and a propus socket having a channel. The propus socket is rigidly mounted below the dactyl plunger, and the dactyl plunger is received into the propus socket when the dactyl plunger is in the closed position. The cavitation generation device can also include a torsion spring that biases the dactyl plunger into contact with the propus socket. In another embodiment, the present disclosure pertains to a method of inducing a cavitation including biasing a dactyl plunger via a torsion spring, and rotating the dactyl plunger, by action of the torsion spring, into a propus socket. The propus socket includes a nozzle-shaped channel. The method further includes ejecting a socket cavity volume through the nozzle-shaped channel thereby inducing a cavitation event.

Abstract: Systems and methods for prediction of state of charge (SOH), state of health (SOC) and other characteristics of batteries using acoustic signals, includes determining acoustic data at two or more states of charge and determining a reduced acoustic data set representative of the acoustic data at the two or more states of charge. The reduced acoustic data set includes time of flight (TOF) shift, total signal amplitude, or other data points related to the states of charge. Machine learning models use at least the reduced acoustic dataset in conjunction with non-acoustic data such as voltage and temperature for predicting the characteristics of any other independent battery.

Abstract: Novel cell penetrating agents for intracellular delivery of desired cargo, including proteins. Use of cell penetrating agents to deliver cargos to the interior of cells and cellular compartments and organelles is transformative for diagnostic, therapeutic, and research processes.

Abstract: The present invention relates to novel compositions and methods for reducing or eliminating the thrombogenicity of a graft by modifying the graft with a cell-derived extracellular matrix lacking thrombospondin-2 (TSP2-null ECM) to render it non-thrombogenic when transplanted to a subject in need thereof. The invention also provides a method for improving the biocompatibility of a medical device or an implant by modifying the medical device or implant with a cell-derived TSP2-null ECM, whereby the medical device or implant is rendered non-thrombogenic and pro-migratory.

Abstract: A double attention network (DANet)-based drone patrol and inspection system for coastline floating garbage, including: an image acquisition module configured to shoot a video of a coastline in need of patrol and inspection by using a drone, and obtain an image from the video; a feature extraction module configured to extract shallow features and deep features, fuse the shallow features and the deep features to obtain a shared feature, and finally output a panoramic recognition result; a network training module configured to perform training on the labeled image so that the network can recognize the coastline and floating garbage; and a path correction module configured to adjust a flying direction of the drone.

Abstract: The present invention relates to a proton ceramic fuel cell which has a hydrogen-permeable film as an anode and in which an electrolyte material is BaZrxCe1-x-zYzO3 (x=0.1 to 0.8, z=0.1 to 0.25, x+z?1.0) (BZCY). An electron-conducting oxide thin film having a film thickness of 1-100 nm is present between a cathode and an electrolyte comprising the material. The present invention also relates to a method for producing a proton ceramic fuel cell having a hydrogen-permeable film as an anode. The method comprises forming a thin film having a thickness of 1-100 nm between a cathode and an electrolyte comprising BZCY, the thin film comprising an electron-conducting oxide. The present invention provides a novel means for improving the output of a PCFC in which BZCY is used in an electrolyte material, and provides a PCFC having an output that exceeds a benchmark of 0.5 W cm?2 at 500° C.

Abstract: Systems and connectors for hemostasis valves and associated methods are disclosed herein. According to an aspect, a connector for a medical device includes a body defining a first opening, a second opening, and a channel. The channel provides passage between the first opening and the second opening. The body is configured to engage an inlet port of a hemostasis valve at the first opening when the body and the hemostasis valve are arranged in one or more positions with respect to each other. The connector also includes a clip attached to the body and configured to fasten the body to the hemostasis valve when the body and the hemostasis valve are arranged in one of the positions.

Abstract: Compounds of formula: are useful as antitumor agents. In these compounds, R10 is (a) (C1-C10) hydrocarbyl, (C1-C10)halohydrocarbyl, (C1-C6)hydroxyalkyl, or or R10 is (b) in which Q and A are linkers and Ar is optionally substituted monocyclic or bicyclic aryl or heteroaryl.