Abstract: The present disclosure provides AAV8 vectors and variants thereof that express nucleic acids sharing identity to a codon optimized NAGLU (coNAGLU) that improves transduction and distribution in brain cells and will improve disease outcomes in the Mucopolysaccharidoses IIIB (MPS IIIB) mouse model. The present disclosure also provides methods of treatment of a subject, and methods of transducing one or more brain cells, by administering these vectors, as well as uses of these vectors in the manufacture of medicaments for treatment. The present disclosure also provides compositions and host cells comprising rAAV vectors and rAAV particles that express a coNAGLU heterologous nucleic acid and confer enhanced transduction efficiency in human cells, such as brain cells (e.g., neurons). These compositions may be administered to a subject in need thereof.

Abstract: A fiber optic temperature sensor, a sensing head structure, and a manufacturing method are provided. The fiber optic temperature sensor includes a broad spectrum light source, a first fiber optic coupler, a spectrometer, a first sensing interferometer, and a second sensing interferometer. The first sensing interferometer and the second sensing interferometer have opposite temperature responses. A first free spectral range corresponding to the first sensing interferometer is close to but not equal to a second free spectral range corresponding to the second sensing interferometer. In the fiber optic temperature sensor, two sensing interferometers both sensitive to temperature are used, and the two sensing interferometers have opposite temperature responses, thereby achieving an enhanced vernier effect, and improving the sensitivity of temperature measurement.

Abstract: Provided is a method for producing, from a carboxylic acid ester, a corresponding ether. In the method, the reaction product and catalyst can be easily separated, and a large amount of salt waste or hazardous waste is not discharged. The method for producing an ether includes reducing a carboxylic acid ester with molecular hydrogen in the presence of the following Catalyst and producing the corresponding ether, in which the —C(?O)O— group of the carboxylic acid ester has been converted to a —CH2O— group: Catalyst: a catalyst in which the following M1 and M2 are supported as metal species on the following Support. M1: platinum, ruthenium, rhodium, palladium, or iridium. M2: molybdenum, rhenium, tungsten, or vanadium. Support: zirconium oxide, titanium oxide, cerium oxide, or hydroxyapatite.

Abstract: A collaborative filtering recommending apparatus includes an initial-embedding generation module configured to generate first and second initial embeddings; a dual co-evolving ordinary differential equation module comprising first and second ordinary differential equation processing members which repeat, for a specific time, a process of receiving first and second previous embeddings, respectively, and outputting first and second subsequent embeddings, respectively, through mutual influence as a learning time elapses, the first and second previous embeddings initially corresponding to the first and second initial embeddings, respectively; and a final-embedding generation module configured to generate a first final embedding by cumulative summing the first previous and subsequent embeddings, and to generate a second final embedding by cumulative summing the second previous and subsequent embeddings.

Abstract: A medical implant is adapted to be implantable within a gallbladder in order to limit movement of any gallbladder stones present within the gallbladder. The medical implant includes a resilient body that is adapted to fit within the interior volume of the gallbladder and a plurality of pores that are formed within the resilient body. The pores are adapted to allow bile to flow through the pores.

Abstract: A contextual visual-based synthetic-aperture radar (SAR) target detection method and apparatus, and a storage medium, belonging to the field of target detection is described. The method includes: obtaining an SAR image; and inputting the SAR image into a target detection model, and positioning and recognizing a target in the SAR image by using the target detection model, to obtain a detection result. In the present disclosure, a two-way multi-scale connection operation is enhanced through top-down and bottom-up attention, to guide learning of dynamic attention matrices and enhance feature interaction under different resolutions. The model can extract the multi-scale target feature information with higher accuracy, for bounding box regression and classification, to suppress interfering background information, thereby enhancing the visual expressiveness.

Abstract: An image sensor integrated with a convolutional neural network computation circuit is provided. The image sensor includes: a pixel array including pixels divided into pixel groups, wherein each pixel converts a light signal into a PWM signal; a convolution computation circuit controlling a turn-on time of a corresponding weighted current according to the first PWM signal of each pixel, and accumulating the weighted currents into an integrated current; a comparison circuit converting the integrated current into a second PWM signal and comparing it with that of an adjacent pixel group to output a larger one; and a classification circuit quantizing the second PWM signal to a quantization value according to a weight of a node in a fully-connected layer corresponding to each pixel group, accumulating the quantization values of all pixel groups into a feature value, and comparing the feature value with a feature threshold to obtain a classification result.

Abstract: Ultra-high temperature carbide (UHTC) foams and methods of fabricating and using the same are provided. The UHTC foams are produced in a three-step process, including UHTC slurry preparation, freeze-drying, and spark plasma sintering (SPS). The fabrication methods allow for the production of any kind of single- or multi-component UHTC foam, while also providing flexibility in the shape and size of the UHTC foams to produce near-net-shape components.

Abstract: Disclosed is an optimizing method for multi-source municipal solid waste combinations based on machine learning, including obtaining relevant property data, classifying the feature variables and obtaining a raw materials pre-combination from the classified feature variables according to a classification ratio, followed by cooperative combustion treatment to obtain data after combustion, summarizing the obtained data into a database, constructing a matrix of raw material components, operating conditions and pollutant distribution according to the database, obtaining matrix data; performing principal component analysis on the matrix data, constructing an information processing model, obtaining a data set of samples; carrying out training according to the data set to construct a relational model, obtaining processed parameters; training the obtained processed parameters to construct a regression module, an optimal parameter, and performing regression calculation using the optimal parameter together with the matr

Abstract: Provided herein are methods of tracking molecular dynamics in three dimensions. In some embodiments, the methods include introducing an incident light toward a second surface of a substrate to induce a plasmonic wave at least proximal to a first surface of the substrate. A population of particles is connected to the first surface of the substrate via one or more first biomolecules. In some embodiments, the methods also include detecting a change in position of the particles in the population along at least three dimensions over a duration from a change in intensity of the incident light reflected at an interface of the first surface of the substrate. Related systems and computer readable media are also provided.

Abstract: In an embodiment, the invention provides a method and reagents for detection of ?-herpesvirus circRNA. In another embodiment, the invention provides a method and reagents for detection of EBV circRNA. In still another embodiment, the invention provides a method and reagents for detection of KSHV circRNA. The method can be expanded to other herpesviruses and even non-herpesviruses that generate circRNA upon cellular infection.

Abstract: Provided are a scalable analog passive intermodulation (PIM) module, a method of controlling analog PIM, a signal processing circuit, and a sensor device. The scalable analog PIM module includes a first plural number of digital-to-analog converters (DACs), a first plural number of static random access memory (SRAM) calculators connected to the first plural number of DACs, at least one analog-to-digital converter (ADC) connected to the first plural number of SRAM calculators and configured to convert an analog convolution result signal into digital convolution data, and an analog PIM controller configured to output an enable control signal for enabling a second number, which is equal to or less than first plural number, of SRAM calculators among the first plural number of SRAM calculators to the first plural number of SRAM calculators on the basis of the convolution data output from the ADC.

Abstract: Provided are SpdE polypeptides and variants and nucleic acids encoding the SpdE polypeptides and variants. Also provided are vectors including one or more nucleic acids encoding a SpdE polypeptide or variant and cells including a nucleic acid encoding the SpdE polypeptide or variant, as well as cells expressing a SpdE polypeptide or variant and compositions including such cells and a pharmaceutically acceptable carrier. Finally, methods of detecting presence and/or amount of one or more amino acids in a sample are provided. The methods include contacting the sample with a SpdE protein, measuring diguanylate cyclase activity of the SpdE protein; and comparing the diguanylate cyclase activity of the SpdE protein to a control. The methods can utilize isolated SpdE protein or a cell expressing a SpdE protein.

Abstract: A microdroplet-based three-dimensional (3D) laser printing system, which includes a laser beam subsystem, a transparent donor substrate, voxel arrays, and the receiver substrate. By irradiating the voxel array with a pulsed laser beam deriving from the laser beam subsystem through the transparent donor substrate, the voxel array is melted and driven away from the transparent donor substrate to generate the ejecting microdroplet array and then deposited onto the receiver substrate. The 3D microstructure is printed in parallel by sequentially irradiating the voxel array and controlling the depositing locations of microdroplet arrays onto the receiver substrate. The system can avoid the satellite microdroplets generating, improve the printing efficiency and resolution, and obtain a wide process window.

Abstract: The adjuvanted conjugate opioid vaccine described herein is a conjugate of a protein carrier and at least one opioid backbone component or hapten conjugated thereto, admixed with at least one adjuvant. Anti-opioid effects are demonstrated after administration of a vaccine made up of the CRM197 protein carrier linked to a FEN backbone, combined with adjuvants such as dmLT or LTA1.

Abstract: A pharmaceutical composition comprising a nucleic acid molecule encoding a variable domain deleted E2 polypeptide of HCV (e.g., E2Delta123). The composition is suitable for use, for use, or when used, in the treatment or prevention of HCV infection. The nucleic acid molecule may be DNA or RNA or a modified or synthetic form, or contained within a plasmid, a viral or non-viral vector for vaccination, a polynucleotide expression cassette, or a cell for vector propagation. Methods of administration as prime and boost vaccinations are also provided.

Abstract: The present disclosure relates to PD-1 variants having minimal mutations for enhancing binding ability to PD-L1. The PD-1 variants of the present disclosure have fewer mutations than existing PD-1 and PD-1 variants and have significantly increased binding ability to PD-L1 compared to the existing variants, thereby solving the problem of immunogenicity. In addition, since these variants are very small-sized proteins as compared to existing antibody therapeutic agents, PD-1/PD-L1 binding of tumors and immune cells in a tumor micro-environment can be effectively inhibited, and since the problem of low binding ability of PD-L1 to existing PD-1 has been solved, the therapeutic effect thereof as a therapeutic agent can be significantly improved. These variants can also be used as an imaging agent for detecting the expression level of PD-L1.

Abstract: The present disclosure belongs to the technical field of food processing, and in particular relates to a rapid and non-destructive quantitative evaluation method for taste characteristics of fried rice. The method includes three steps: constructing a quantitative model of seasoning for the fried rice, constructing a model for identifying types of materials of the fried rice, and performing quantitative characterization of the taste characteristics of the fried rice. The quantitative model of the seasoning is established based on sensitivity of a spectral signal to changes of the content of seasoning liquid. An analytic equation of key parameters of the quantitative model of the seasoning is established according to characteristics that the total amount of the seasoning in finished fried rice is equal to the total amount of the seasoning added during frying of the fried rice. The quantitative model of the seasoning is rapidly constructed by solving the equations.

Abstract: An overlapping and progressive forming method for a high-performance multi-element NiAl-based alloy tubular part, including: winding continuously flexible substrates of Ni and Al, and alloying coating continuously or selectively along a width direction or a rolling direction to obtain coated flexible substrates; winding continuously the coated flexible substrates on an outer surface of a core roller according to a sequence of Ni above and Al below to form a Ni/Al laminated structure having a plurality of layers with an outermost layer being a Ni layer, and consolidating with ultrasonic with assistance of a pulse current to combine the continuously wound flexible substrates into a laminated tube blank; and placing the laminated tube blank into a mold, applying a pulse current to both ends of the laminated tube blank for hot fluid high-pressure forming, and synthesizing in-situ to prepare the tubular part with assistance of the pulse current.

Abstract: A system for controlling multicomputer interaction with deep learning is disclosed that includes a controller system that is configured to generate one or more first user-controllable avatars on an interaction field, where the first avatars include movement controls and prompt functionality that is controllable by a first user to cause the first avatar to generate a prompt. A client system is configured to generate a second user-controllable avatar on the interaction field, where the second avatar includes movement controls and response functionality that is controllable by a second user to cause the second avatar to generate a response to the prompt. A deep learning processing system is configured to receive the prompt and the response and to process the prompt and the response to generate a score and to assign the score to one of two or more categories associated with the second user.