Abstract: A sensor element includes an element main body having an oxygen ion-conducting solid electrolyte body, a detection electrode which is disposed on an outer surface of the element main body and contains Pt and Au, a reference electrode which is disposed in the element main body, a connecting terminal for detection electrode which is disposed on the outside of the element main body, a lead portion for detection electrode which contains Pt, is disposed on the outside of the element main body, and electrically connects between the detection electrode and the connecting terminal for detection electrode, a lower insulating layer which is disposed between the lead portion for detection electrode and the element main body and insulates the two from each other, and an upper insulating layer which covers a surface of the lead portion for detection electrode and has a porosity of 10% or less.

Abstract: [Problem to be Solved] A biosensor having high detection sensitivity and detection specificity is provided. [Solution] There is provided a biosensor comprising: an identification substance capable of binding to a substance to be detected; and an electrode charged with a charge of the identification substance, and detecting a change in a charge density of the electrode caused by binding of the substance to be detected to the identification substance, wherein a polymer layer in which a molecular template having a structure complementary to a molecular structure of the substance to be detected is formed is formed on all or part of a surface of the electrode, the identification substance is contained in the polymer layer, and the polymer layer is an ultrathin film layer.

Abstract: Provided is a method for measuring a component of a biological sample with a biosensor provided with: a capillary for introducing the biological sample; an electrode part including a first electrode system that includes a first working electrode and a first counter electrode in the capillary; and a reagent part disposed so as to be in contact with the electrode part, the reagent part containing an enzyme and a mediator, and the method including a step of starting voltage application for a duration longer than 0 second and up to 0.7 second to the first electrode system within 0 second to 0.5 second after detection of the introduction of the biological sample to obtain a hematocrit value based on a current value obtained thereby.

Abstract: Nanopore-based molecular analysis systems including a disposable well array, methods of analysis of biomolecules using nanopore molecular analysis systems, and methods of fabricating disposable well arrays are provided.

Abstract: A test strip includes a working electrode including a conductive layer and an electro-catalytic layer deposited on the conductive layer. A method for simultaneously detecting a glucose concentration and a percentage of glycated hemoglobin in a single test strip includes following steps: providing a blood sample, providing the aforementioned test strip, performing a sample injecting step, performing an initial step, performing a first detecting step, performing a second detecting step, performing a third detecting step, performing a first analyzing step, and performing a second analysis step.

Abstract: An electro-optical stimulation and recording system is disclosed, including a substrate and a plurality of wells coupled to the substrate. The system also includes at least one electrode set disposed proximate a respective one of the plurality of wells, wherein the electrode set comprises at least one electrode configured to collect an electric signal associated with at least a portion of the respective well. The system also includes a light-emitting element set corresponding to a respective one of the wells and configured to deliver optical stimulation to at least a portion of the respective well.

Abstract: This disclosure provides techniques for extending useful life of a reference electrode, as well as a novel voltametric system and measurement cell design and related chemistries. An automated, repeatable-use system features a reference electrode that directly immerses a metallic conductor into an analyte, with electrolytes (e.g., chlorides) used for measurement being separately added and removed for each measurement cycles; the metallic conductor can optionally be left exposed to clean dry air in between measurements. In one implementation, the system can be restricted to application with specific analytes (e.g., ground water) that are known in advance to be free of substances that could degrade reference electrode use or lifetime. Cleaning solutions can optionally be used that would not be practical with conventional (insulated) reference electrode designs.

Abstract: A gas sensor includes a sensor element, metal terminals, a tubular separator holding the metal terminals, lead wires connected to rear ends of the metal terminals, and an elastic member that holds the lead wires extending rearward from the separator. Each metal terminal includes a forward end portion electrically connected to an electrode portion, a central portion, and a lead wire connection portion. The forward end portion or the central portion is held by the separator. The central portion and the lead wire connection portion are connected to each other through a neck portion. The rearward-facing rearmost surface of the central portion is engaged with a forward-facing surface of the separator, and the circumferential length of a first connection portion between the central portion and the neck portion is longer than the circumferential length of a second connection portion between the lead wire connection portion and the neck portion.

Abstract: A biosensor has the following components: a sensor base with an insulating substrate and at least one electrically conductive working electrode arranged thereon, in particular formed from separately controllable interdigital electrodes, reduced graphene applied to at least one working electrode, a spacer covalently bound to the reduced graphene, and an antibody fragment Fab covalently bound to the spacer. There is also described a process for producing the biosensor, a biochip equipped with the sensor and a method of detecting an analyte using the biosensor/biochip.

Abstract: A method of making an electrochemical sensor strip that includes: depositing a first electrode on a base; depositing a second electrode on the base; applying a first layer onto the first electrode; and applying a second layer onto the second electrode. The first layer includes an oxidoreductase and a mediator. The second layer includes a soluble redox species.

Abstract: A structure of an electrochemical unit includes a substrate, a first metal layer disposed on the substrate, and an array of electrochemical cells disposed on the first metal layer. The array of the electrochemical cells includes a plurality of electrochemical cells. Each of the electrochemical cells includes the first metal layer disposed on the substrate, a first electrode disposed on the first metal layer, a polymer layer disposed on the substrate and adjacent to the first metal layer and the first electrode. A second metal layer is disposed on the polymer layer, and a second electrode is disposed on the second metal layer. A pore is constituted between the polymer layers of every the two electrochemical cells. A cavity located above the first electrode is defined between every the two electrochemical cells, wherein the cavity is communicated with the pore.

Abstract: Described herein are nanopore devices as well as methods for assembling a nanopore device including one or more nanopores that can be used to detect molecules such as nucleic acids, amino acids (proteins), and the like. Specifically, a nanopore device includes an insulating layer that reduces electrical noise and thereby improves the sensing resolution of the one or more nanopores integrated within the nanopore device.

Abstract: A sensor using electrophoresis may include a microfluidic channel and electrodes on opposite sides of the microfluidic channel to generate an electric field across, or normal to, the channel. The electric field may be used to drive charged particles of material, particularly material suspended in fluid in the microfluidic channel, toward or away from the one of the electrodes. The electric field may be modulated to allow material to continue flowing through the microfluidic channel, to remove non-target material, or to measure another target material.

Abstract: Methods and apparatus providing for the isolation of an unknown mutation from a sample comprising wild type nucleic acids and mutated nucleic acids through the application of time-varying driving fields and periodically varying mobility-altering fields to the sample within in an affinity matrix.

Abstract: An electrochemical gas sensor (10) has a housing (20), a working electrode (51), a counterelectrode (52) and a reference electrode (53). The housing (20) has an electrolyte reservoir (30), a gas inlet orifice (21) and at least one gas outlet orifice (22). The electrolyte reservoir (30) is filled with a liquid electrolyte (40). The gas sensor (10) has a counterelectrode carrier (26). The counterelectrode (52) is suspended on the counterelectrode carrier (26) in such a way that the counterelectrode (52) is suspended in the electrolyte reservoir (30) and the electrolyte (40) flows around the counterelectrode (52) on all sides. Preferably, the electrolyte includes (I) a solvent, e.g. water, propylene carbonate, ethylene carbonate or mixtures thereof; (ii) a conductive salt, especially an ionic liquid; and/or (iii) an organic mediator, for example substituted quinones, anthraquinones, etc.

Abstract: In one aspect, methods of biomolecular analysis are described herein. Briefly, a method comprises providing a composition comprising glycosaminoglycans and contacting the composition with a membrane comprising at least one nanopore. An electric field is applied across the nanopore, and data of glycosaminoglycan translocation events through the nanopore are recorded. A molecular weight distribution of the glycosaminoglycans is derived from the data.

Abstract: The present disclosure discloses a multi-droplet sensing and actuation system, for use in a digital microfluidic chip operation wherein a linearly independent alternating current signal is applied to each discrete actuation electrode thus encoding the electrode's identity. The combined measured impedance signal from multiple channels is then processed to decode an impedance measurement for the volume between each discrete actuation electrode and its corresponding conductive counter electrode region, where the sensed impedance is inversely proportional to an amount of liquid within the volume.

Abstract: Disclosed is an ammonia sensor element for detecting ammonia in a gas under measurement, including two mixed-potential-type ammonia detection cells that respectively include first and second ammonia detection electrodes. The first ammonia detection electrode has a metal composition containing Au as a predominant component. The second ammonia detection electrode has a metal composition containing Au as a predominant component. The metal compositions of the first and second ammonia detection electrodes are different from each other.

Abstract: Methods and apparatus for detecting an analyte using fluorinated phthalocyanines is disclosed herein. A method for detecting an analyte includes illuminating an analyte solution which contacts an electrode comprising a conductive material having a photosensitizer coupled thereto to generate a reactive oxygen species, wherein the photosensitizer is a fluorinated pthalocyanine having a metal or a non-metal center, oxidizing an analyte present in the analyte solution with the reactive oxygen species to form an oxidized analyte, and detecting a current resulting from the reduction of the oxidized analyte at the electrode.

Abstract: Apparatuses and methods for whole column imaging detection (WCID) capillary isoelectric focusing (CIEF). The apparatus includes a separation capillary having a separation inner diameter and a separation outer diameter; a base, wherein the separation capillary is anchored to the base; an inlet transfer capillary having an inlet inner diameter and an inlet outer diameter; and an outlet transfer capillary having an outlet inner diameter and an outlet outer diameter. The inlet transfer capillary, the separation capillary, and outlet transfer capillary are configured to be in fluidic communication with each other. The separation inner diameter exceeds the outlet inner diameter.