Abstract: The present invention relates to a clearing technique which uses a solution containing at least one of a compound having a delipidation ability, a compound having a biochrome decoloring ability, a compound having a decalcification ability, a compound having a refractive index adjusting ability, and a compound having a tissue swelling ability. The clearing technique is suitable for use in high-throughput and low-magnification imaging which involves a simple process.

Abstract: Two rhodamine reversible fluorescent sensor derivatives, L1 and L2, bearing 2-methoxy-1-naphthaldehyde and 5-bromo-3-methoxy salicylaldehyde units were synthesized using microwave-assisted organic synthesis and used for selective and sensitive reversible sequential fluorescence detection of aluminum ion (Al3+) and azide (N3?) in aqueous acetonitrile solution via the fluorescence spectral changes. Stoichiometry and binding mechanisms for both sensors are well characterized and established by the respective spectroscopic techniques. L1 and L2 sensors are useful for the analysis of Al3+ and N3? in environmental samples and biological studies.

Abstract: A chromogenic assay includes a substrate comprising an array of 5 or more dyes which react with volatile organic compounds, wherein the dyes are chromogenic when reacted with volatile organic chemical biomarkers, wherein the volatile organic chemical biomarkers comprise acids, alcohols, aldehydes, alkenes, amines, antioxidants, aromatic compounds, esters, ethylene, lactones, ketones, organosulfur compounds, sulfides, reactive oxygen species, terpenes, or a combination thereof. A method of detecting volatile organic chemical biomarkers includes contacting the chromogenic assay with a sample or sample headspace, wherein the sample or sample headspace is suspected of containing volatile organic chemical biomarkers, and identifying, based on a colorimetric pattern on the chromogenic assay after contacting, the source of the volatile organic chemical biomarkers. Also included are articles and systems including the chromogenic assay.

Abstract: The invention relates generally to compositions and methods for the detection of zinc. Provided herein is a class of zinc-responsive probes with tunable photophysical properties that can be modified for coupling to a solid support or other chemical moieties. In particular, modifications to the 5-position of the BODIPY core allows for alteration of probe properties and functionalities.

Abstract: A composition, method and kit for quantifying the concentration of one peracid or the total concentration of two or more peracids in a water source are disclosed. In particular, an oxidation sensitive or peroxycarboxylic acid selective leuco dye is used to react only with one or more peroxycarboxylic acids in a water source but not with peroxide usually co-existing in the water source. Not only does the oxidation sensitive or peroxycarboxylic acid selective leuco dye react with peracid(s) but also the reaction generates a color species that can be used visually or through absorbance measurement to quantify the peracid concentration. The method disclosed here is not a titration method, it involves a simple mixing step. The kit or composition disclosed here does not utilize a catalyst, such as iodine.

Abstract: The disclosure provides probes comprising dipyrromethane-BF2 derivatives which exhibits different fluorescent spectral properties when conjugated to the amino acids, compositions and kits comprising same. The disclosure also provides methods for detecting/identifying amino acids and sequencing polypeptide molecules by conjugating a dipyrromethane-BF2 derivative which exhibits different fluorescent spectral properties when conjugated to the amino acids.

Abstract: Disclosed are a water-soluble fluorescent probe and nanoparticles with an aggregation induced emission (AIE) effect and preparation methods and the application. The fluorescent probe molecule includes one imidazole ring substituted by two methoxyphenyls and one phenyl and two quaternary ammonium salt structures. It has good dispersion in water, may form micelles. It has the characteristics of AIE effect and a large Stokes shift etc. It is suitable for fluorescence detection. Under the induction of lysophosphatidic acid, the probe can be self-assembled so as to aggregate and generate yellow fluorescence. Lysophosphatidic acid may be quantitatively analyzed by measuring the intensity of emitted fluorescence.

Abstract: A cartridge capable of a centrifugation and an automatic analysis according to an embodiment of the present invention includes: at least two or more sample wells, a centrifugation well, a measuring well, and a tip receiving well, in which a container is formed into a lower portion along a circumference of a circular plate with a predetermined interval, wherein the centrifugation well is sealed with one lid paper along with at least two or more of the sample wells, a blood injection unit is further formed inside of a radius direction for the centrifugation well, the blood injection unit has an outer blood injection hole formed to be spaced inside the centrifugation well in the radius direction, an inclined passage that communicates with the centrifugation well from the outer blood injection hole, and a cover integrally attached to the outer blood injection hole to open and close the blood injection hole.

Abstract: A rotary analysis apparatus and related methods are disclosed. The apparatus generally includes a rotary machine operable to rotate or spin a removable disk-type analytical cartridge. The cartridge includes a plurality of fluidly isolated processing trains for processing multiple samples simultaneously. Each process train includes an extractant mixing chamber, slurry filtration chamber, supernatant collection chamber, and reagent mixing chamber in fluid communication. In one use, soil sample slurry is prepared and added to the extractant mixing chamber. The slurry is mixed with an extractant by rotating the cartridge to separate out an analyte from the mixture. A sediment filter in the filtration chamber deliquifies and traps soil particles to produce clear supernatant. A color changing reagent or fluorescent agent may be mixed with the collected supernatant for subsequent colorimetric, fluorescent, turbidimetric, or other type of analysis.

Abstract: Provided herein are processes for preparing fluorescent 1-cyano-2-substituted isoindole compounds or N-substituted phthalazinium compounds, comprising reacting an aromatic dialdehyde or aromatic aldehyde-ketone compound with a material that contains primary amino or hydrazine groups, and assaying methods involving the processes thereof.

Abstract: Embodiments of the present disclosure pertain to methods of monitoring an environment for the presence of a solvent by: (i) exposing the environment to a luminescent compound, where the relative luminescence emission intensity of the luminescent compound changes upon interaction with the solvent; and (ii) monitoring a change in the relative luminescence emission intensity of the luminescent compound, where the absence of the change indicates the absence of the solvent from the environment, and where the presence of the change indicates the presence of the solvent in the environment. The luminescent compounds include a phosphorous atom with one or more carboxyl groups, where the carboxyl groups are coordinated with one or more metallic ions (e.g., lanthanide ions and yttrium ions). The present disclosure also pertains to sensors for monitoring an environment for the presence of a solvent, where the sensors include one or more of the aforementioned luminescent compounds.

Abstract: Compositions for testing soil to measure one or more of the following properties of soil: pH, buffer pH, potassium, phosphorus, calcium, and/or magnesium. Test methods for testing soil to measure one or more of the following properties of soil: pH, buffer pH, potassium, phosphorus, calcium, and/or magnesium. A multichamber cartridge containing two or more of the compositions in separate chambers.

Abstract: The synthesis and characterization of a platinum (II) terpyridine system tethered with a latent organic dye of rhodamine derivative as colorimetric and fluorescent sensory moiety has been reported to show selective Hg2+ ion sensing behavior. The interplay of aggregation/de-aggregation behavior of the alkynylplatinum(II) terpyridine complex and the ring-opening process of rhodamine derivative has been investigated. The spectral change of aggregation NIR emission at 800 nm and rhodamine fluorescence at 585 nm provides a possible ratiometric luminescence measurement. Morphological studies from TEM and SEM images showing nanospherical structures confirmed the aggregation in the absence of Hg2+ ion.

Abstract: A method for analyzing a diagnostic test may include receiving an image depicting a diagnostic test, wherein the diagnostic test comprises a test region indicating a test result, validating quality of the image, locating a test region image portion of the image depicting the test region of the diagnostic test; and predicting the test result based on the test region image portion. Furthermore, a method for facilitating analysis of a diagnostic test may include receiving one or more images depicting one or more control markings on a scan surface, wherein the one or more control markings are representative of one or more predetermined test results for the diagnostic test, and verifying detection of the one or more control markings in the one or more images using at least one computer vision technique.

Abstract: Cross-linked polymer networks that are at least partially conjugated (e.g., phenylene vinylene polymer networks). The cross-linked polymer networks may be thin-films disposed on a substrate. The cross-linked polymer network may be covalently bonded to the substrate. The cross-linked polymer networks can be used, for example, in methods of detecting explosives (e.g., RDX (cyclotrimethylenetrinitramine)) and degradation products thereof.

Abstract: The invention relates generally to compositions and methods for the detection of zinc. Provided herein is a class of zinc-responsive probes with tunable photophysical properties that can be modified for coupling to a solid support or other chemical moieties. In particular, modifications to the 5-position of the BODIPY core allows for alteration of probe properties and functionalities.

Abstract: Compositions, kits, and methods of using the kits and compositions to determine the hardness of and/or magnesium concentration in a solution are described. The kit can include a lyophilized composition that has an absorbance at a detectable wavelength in response to the hardness of the solution and or the concentration of magnesium in the solution.

Abstract: The teachings herein provide for a method of analyzing testosterone using mass spectrometry. The method entails combining in a vial or well, a tagging reagent that is reactive with testosterone, an aqueous precipitation agent that precipitate proteins from solution, and an internal standard solution, the internal standard solution containing a known concentration of an isotopically enriched testosterone and then adding to the vial or well, a sample containing or suspected to contain testosterone. The vial can then be mixed to cause simultaneous precipitation of proteins and reaction of any testosterone present with the tagging reagent to form a mixture of a precipitate and a liquid solution. The liquid solution can then be separated from any precipitate and then analyzed for testosterone using liquid chromatography tandem mass spectrometry.

Abstract: Described are precursor compounds and methods for atomic layer deposition of films containing scandium(III) oxide or scandium(III) sulfide. Such films may be utilized as dielectric layers in semiconductor manufacturing processes, particular for depositing dielectric films and the use of such films in various electronic devices.

Abstract: A microfluidic structure in which a plurality of chambers arranged at different positions are connected in parallel and into which a fixed amount of fluid may be efficiently distributed without using a separate driving source, and a microfluidic device having the same. The microfluidic device includes a platform having a center of rotation and including at least one microfluidic structure. The microfluidic structure includes a sample supply chamber configured to accommodate a sample, a plurality of first chambers arranged in a circumferential direction of the platform at different distances from the center of rotation of the platform, and a plurality of siphon channels, each of the siphon channels being connected to a corresponding one of the first chambers.