Patents Examined by Emily R. Berkeley
  • Patent number: 10753915
    Abstract: A method for analyzing and quantifying a panel of drugs in a clinical sample comprises: trapping a first portion of drug parent compounds and their metabolites on a first chromatographic column; trapping a second portion of the drug parent compounds and their metabolites on a second chromatographic column; separately eluting the first and second portions of the drug parent compounds and their metabolites from the first and second chromatographic columns; detecting concentrations of each of the drug parent compounds and metabolites eluted from each of the first and second chromatographic columns with a detector; and summing the detected concentration of each drug parent compound together with the detected concentrations of all of its respective analytes so as to derive a respective total concentration of each drug in the sample.
    Type: Grant
    Filed: July 27, 2016
    Date of Patent: August 25, 2020
    Assignee: Thermo Finnigan LLC
    Inventors: Joseph Herman, Sarah J. Fair, Dayana Argoti
  • Patent number: 10746639
    Abstract: A method of extracting an analyte from a sample is described. The sample is added to a sample container. A liquid solvent and a gas are added into the sample container. The addition of the gas is controlled to establish an elevated pressure within the sample container. The liquid solvent is heated to an elevated temperature that is below the boiling temperature of the liquid solvent at the elevated pressure. A type of gas is used that does not transition to a supercritical fluid at the elevated temperature and pressure used in the extraction process. The analyte can dissolve from the solid sample into the liquid solvent. Next, at least a portion of the liquid solvent containing the dissolved analyte can be removed from the sample container for subsequent analysis.
    Type: Grant
    Filed: August 10, 2016
    Date of Patent: August 18, 2020
    Assignee: Dionex Corporation
    Inventors: Kannan Srinivasan, SM Rahmat Ullah
  • Patent number: 10746662
    Abstract: A method and system incorporating a process for analyzing a sample to be tested to determine the presence of or to quantify an analyte in the sample. The process employs a reaction which produces a reaction medium derived from the sample that possesses fluorescence properties, in response to illumination by a light source. The process includes illuminating the sample and detecting a fluorescent signal, introducing the reaction medium, and illuminating the sample and detecting a second fluorescent signal from the sample. The process further includes processing the two signals to determine which portion of the fluorescent signal is produced solely by the reaction medium.
    Type: Grant
    Filed: December 6, 2018
    Date of Patent: August 18, 2020
    Assignee: BIOMERIEUX
    Inventors: Bruno Colin, Laurent Drazek, Frederic Pinston
  • Patent number: 10750577
    Abstract: A fluidic chip comprising: a sealing layer having an upper surface and a lower surface; and a formed part comprising a generally planar body having a lower surface sealed with the upper surface of the sealing layer, the generally planar body having a number of through holes and a number of wells in fluid communication with the number of through holes, wherein together with the upper surface of the sealing layer, the number of through holes and the number of wells respectively define a number of fluid inlets and a number of fluid chambers in fluid connection with each other in the fluidic chip.
    Type: Grant
    Filed: October 7, 2017
    Date of Patent: August 18, 2020
    Assignee: CELL ID PTE LTD
    Inventor: Lye Hock Sim
  • Patent number: 10732167
    Abstract: A multi-phase system includes a phase-separated solution comprising at least two phases, each phase having a phase component selected from the group consisting of a polymer, a surfactant and combinations thereof, wherein at least one phase comprises a polymer, wherein the phases, taken together, represent a density gradient. Novel two-phase, three-phase, four-phase, five-phase, or six-phase systems are disclosed. Using the disclosed multi-phase polymer systems, particles, or other analyte of interest can be separated based on their different densities or affinities.
    Type: Grant
    Filed: July 24, 2017
    Date of Patent: August 4, 2020
    Assignee: President and Fellows of Harvard College
    Inventors: Charles R. Mace, Ozge Akbulut Halatci, Ashok A. Kumar, Nathan D. Shapiro, George M. Whitesides
  • Patent number: 10732163
    Abstract: Apparatuses and methods of measuring a hydrogen diffusivity of a metal structure including during operation of the metal structure, are provided. A hydrogen charging surface is provided at a first location on an external surface of the structure. In addition, a hydrogen oxidation surface is provided at a second location adjacent to the first location on the external surface of the structure. Hydrogen flux is generated and directed into the metal surface at the charging surface. At least a portion of the hydrogen flux generated by the charging surface is diverted back toward the surface. A transient of the diverted hydrogen fluxes measured, and this measurement is used to determine the hydrogen diffusivity of the metal structure in service.
    Type: Grant
    Filed: April 28, 2017
    Date of Patent: August 4, 2020
    Assignee: Saudi Arabian Oil Company
    Inventors: Abderrazak Traidia, Mohammed Shahrani, Sebastien A. Duval
  • Patent number: 10725057
    Abstract: The present invention addresses the problem of providing a novel method for the pretreatment of a biological sample containing lenalidomide enantiomer and thereby establishing a simple and accurate method for the quantitative analysis of lenalidomide enantiomer. In the present invention, the racemization and decomposition of lenalidomide enantiomer in a biological sample can be prevented by the deproteinization under acidic conditions of the biological sample containing lenalidomide enantiomer, and the lenalidomide enantiomer can be simply and accurately quantitatively analyzed by subjecting to HPLC the biological sample that has been pretreated in such a way.
    Type: Grant
    Filed: May 21, 2015
    Date of Patent: July 28, 2020
    Assignee: Shiseido Company, Ltd.
    Inventors: Yosuke Tojo, Masashi Mita, Wolfgang Lindner
  • Patent number: 10718746
    Abstract: A water hardness-measuring method and a fouling prevention method for hardness-measuring device include adding a colorant containing Eriochrome Black T and/or Calmagite and a sulfate ester-type anionic surfactant having a bonding unit derived from a sulfate ester including an alkyl group having 8 to 18 carbon atoms that may have one or more substituent in its chemical formula to a sample water.
    Type: Grant
    Filed: February 10, 2015
    Date of Patent: July 21, 2020
    Assignee: KURITA WATER INDUSTRIES LTD.
    Inventor: Junichi Takahashi
  • Patent number: 10712338
    Abstract: In the method of preparing a sample for analysis, a reaction is performed that produces different modified product depending on the sialic acid linkage type when a sialic acid is bound to a sugar chain of an analyte. In this reaction, an analyte containing a sugar chain, an amine containing two or more carbon atoms, and a dehydration-condensation agent are used. The sialic acid linkage type can be identified by analyzing the resulting sample with mass spectrometry etc. This method is applicable not only to free sugar chains but also to glycopeptides and glycoproteins.
    Type: Grant
    Filed: March 31, 2016
    Date of Patent: July 14, 2020
    Assignee: SHIMADZU CORPORATION
    Inventor: Takashi Nishikaze
  • Patent number: 10688489
    Abstract: Fluid storage containers and analysis cartridges for use in assay processes are presented. In addition, systems comprising such storage containers and analysis cartridges and methods of using such containers and cartridges are presented as well. In specific embodiments, fluid storage containers are configured to be coupled to analysis cartridges in a first stage and a second stage.
    Type: Grant
    Filed: February 8, 2017
    Date of Patent: June 23, 2020
    Assignee: LUMINEX CORPORATION
    Inventors: Ross Johnson, Chuck Collins
  • Patent number: 10682622
    Abstract: A micro-fluidic reactor may comprise a photosensitive glass substrate with a plurality of features produced by etching. The features may include micro-channels, micro-lenses, and slots for receiving optical fibers. During operation of the micro-fluidic reactor, the optical fibers may transmit optical signals for measuring characteristics of fluid reagents and reactions taking place. The micro-lenses may focus optical signals from the optical fibers to create an approximately collimated optical path for the optical signals, reducing optical spread and enhancing fiber-to-fiber optical power coupling.
    Type: Grant
    Filed: May 3, 2017
    Date of Patent: June 16, 2020
    Assignee: Board of Trustees of the University of Alabama, for and on behalf of the University of Alabama in Huntsville
    Inventors: William Randall Gaillard, John D. Williams
  • Patent number: 10684201
    Abstract: Fluidic devices and methods associated with mixing of fluids in fluidic devices are provided. In some embodiments, a method may involve the mixing of two or more fluids in a channel segment of a fluidic device. The fluids may be in the form of, for example, at least first, second and third fluid plugs, composed of first, second, and third fluids, respectively. The second fluid may be immiscible with the first and third fluids. In certain embodiments, the fluid plugs may be flowed in series in the channel segment, e.g., in linear order, causing the first and third fluids to mix without the use of active components such as mixers. The mixing of fluids in a channel segment as described herein may allow for improved performance and simplification in the design and operations of fluidic devices that rely on mixing of fluids.
    Type: Grant
    Filed: February 27, 2017
    Date of Patent: June 16, 2020
    Assignee: OPKO Diagnostics, LLC
    Inventors: Matthew Dirckx, Vincent Linder, Jason Taylor
  • Patent number: 10677779
    Abstract: Disclosed is a platelet-testing chip. The platelet-testing chip includes a plurality of sample chambers storing blood samples, stirrers provided in the sample chambers to apply shearing force to the blood samples, a plurality of waste sample chambers provided so as to correspond to the plurality of the sample chambers, microchannels, through which the sample chambers and the waste sample chambers corresponding to each other are independently connected to thus form paths through which the blood samples flow from the sample chambers to the corresponding waste sample chambers, and microbeads which are received in one or more of the plurality of the sample chambers and which are coated with a reagent for activating platelets on an outer surface thereof. When the blood samples are transferred from the sample chambers through the microchannels, the microbeads are transferred together with the blood samples.
    Type: Grant
    Filed: February 23, 2016
    Date of Patent: June 9, 2020
    Assignee: Korea University Research and Business Foundation
    Inventor: Se-Hyun Shin
  • Patent number: 10677811
    Abstract: A sample receptacle including one or more receptacle cavities each having an opening dimensioned such that a liquid within the cavity is retained when the cavity opening is oriented downwardly and/or a gas vent in the base of each cavity sized and positioned to allow gases contained within the cavity to egress whilst preventing the egress of liquid at atmospheric pressure. A sample liquid may be poured into the sample receptacle so that the level of the sample liquid is above each cavity opening and the sample receptacle inverted so as to remove liquid above each cavity whilst retaining sample liquid in each sample receptacle when inverted. This may be used for sample separation or to provide relatively uniform sample volumes to sample wells of a sample container when mated. In another embodiment plungers may be used to eject liquid from receptacle wells via an aperture in the base of each receptacle well.
    Type: Grant
    Filed: February 26, 2016
    Date of Patent: June 9, 2020
    Assignee: Mastaplex Ltd.
    Inventor: Olaf Bork
  • Patent number: 10663458
    Abstract: The present invention relates to a new method for the determination of free copper in serum. In particular to a method with a high degree of sensitivity and accuracy for the determination of free copper in serum samples of patients with Alzheimer's disease and Wilson's disease and other forms of free copper dyshomeostasis. The invention also relates to kits for the determination of free copper in serum comprising filter devices and coumarin fluorescent probe.
    Type: Grant
    Filed: November 7, 2012
    Date of Patent: May 26, 2020
    Assignee: IGEA RESEARCH CORPORATION
    Inventors: Nicola Antonio Colabufo, Rosanna Squitti
  • Patent number: 10661277
    Abstract: A microfluidic device includes a substrate, a microchannel, a plurality of spaced-apart pillars, and a porous nanofiber structure. The substrate has a substrate top surface. The microchannel is indented downwardly from the substrate top surface. The pillars are disposed in the microchannel. Each of the pillars has a pillar top surface that is lower in level than the substrate top surface. The porous nanofiber web structure is formed in the microchannel, and includes a first web portion residing in a space formed among the pillars.
    Type: Grant
    Filed: October 27, 2017
    Date of Patent: May 26, 2020
    Assignee: NATIONAL TAIWAN NORMAL UNIVERSITY
    Inventors: Tien-Li Chang, Chi-Huang Huang, Cheng-Ying Chou
  • Patent number: 10641752
    Abstract: A compact explosive detecting system collects explosive residues in the form of vapor powder. The residues are accumulated on a desorber which is subjected to pyrolysis to release a gaseous sample. The sample is pumped to a detecting system through a metering valve. A luminol cell reacts with the gaseous sample to create chemiluminescence, the light output of which is measured by a photo multiplier tube. The light intensity is indicative of the amount of explosive present. Based on the amount of explosive present, a metering valve is adjusted to pass the gaseous sample into a highly sensitive metal oxide sensor array to detect NO2 from nitrogen containing explosive and CO/CO2 from non nitrogen containing explosive. The metal oxide sensor array reliably selects explosives from those compounds indicating chemiluminescence.
    Type: Grant
    Filed: May 30, 2017
    Date of Patent: May 5, 2020
    Assignee: SCINTREX TRACE CORPORATION
    Inventor: Dao Hinh Nguyen
  • Patent number: 10640513
    Abstract: The present invention relates to a compound for detecting phosgene and DCP (diethyl chlorophosphate) and a composition for detecting phosgene and DCP (diethyl chlorophosphate) comprising the said compound. More precisely, the compound for detecting phosgene and DCP of the present invention can selectively detect phosgene and DCP either in the liquid phase of gas phase by detecting the changes of fluorescence and color development very quickly within a few seconds with nM sensitivity. Therefore, the compound can also be effectively used as an ingredient for the composition and kit for the detection of one or more materials selected from the group consisting of phosgene and DCP.
    Type: Grant
    Filed: February 23, 2017
    Date of Patent: May 5, 2020
    Assignee: EWHA UNIVERSITY - INDUSTRY COLLABORATION FOUNDATION
    Inventors: Juyoung Yoon, Xin Zhou, Ying Hu, Liyan Chen
  • Patent number: 10626141
    Abstract: The present invention provides a method for specifically cleaving a C?-C bond of a peptide backbone and/or a side chain of a protein and a peptide, and a method for determining amino acid sequences of protein and peptide. A method for specifically cleaving a C?-C bond of a peptide backbone and/or a side chain bond of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of at least one hydroxynitrobenzoic acid selected from the group consisting of 3-hydroxy-2-nitrobenzoic acid, 4-hydroxy-3-nitrobenzoic acid, 5-hydroxy-2-nitrobenzoic acid, 3-hydroxy-5-nitrobenzoic acid, and 4-hydroxy-2-nitrobenzoic acid. A method for determining an amino acid sequence of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of the above specific hydroxynitrobenzoic acid to specifically cleave a C?-C bond of a peptide backbone and/or a side chain bond, and analyzing generated fragment ions by mass spectrometry.
    Type: Grant
    Filed: May 12, 2017
    Date of Patent: April 21, 2020
    Assignees: SHIMADZU CORPORATION, NATIONAL UNIVERSITY OF CORPORATION HIROSHIMA UNIVERSITY
    Inventors: Shunsuke Izumi, Koichi Tanaka, Yuko Fukuyama
  • Patent number: 10620171
    Abstract: The present disclosure generally relates to improved methods for separating and analyzing compounds by liquid chromatography, particularly when coupled with mass spectrometry. The methods include the addition of an additive to a mobile phase carrying a sample. The mobile phase additive may be effective for improving peak shapes of targeted analytes in acquired data, and/or eliminating or at least reducing ion suppression. The methods are particularly suited for anionic compounds such as phosphorylated compounds.
    Type: Grant
    Filed: August 31, 2017
    Date of Patent: April 14, 2020
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventors: Jordy J. Hsiao, Hongfeng Yin, Oscar G. Potter