Abstract: A bioreactor for producing hydrogen gas and metabolites and methods for making and using the same. The bioreactor comprises a main reactor chamber, a semipermeable membrane, a sleeve, microorganism, such as cyanobacteria, for producing hydrogen gas and metabolites, a power supply, a substrate medium, a heating member, a plurality of tubing members, a collection reservoir, a pressure-sealed connecter member, a metabolite purifying filter member, a light source, and an agitator. The bioreactor utilizes light, fermentation, and other metabolic processes for the production of metabolites, derived from various microorganisms contained within the bioreactor through respective metabolic pathways.

Abstract: A bioreactor has a container, an end plate, an agitator, a drive unit, and at least one field device arranged in the interior, field device electronics, a data transmission unit arranged on the end plate, and a motor head piece coupled in terms of energy to the drive unit. At least one communication unit is coupled in terms of signaling to the motor head piece and includes an adapter piece, having adapter electronics which are electrically connected to the field device electronics of the field device, and a communicator piece. The adapter piece and the communicator piece are separate components which can be coupled to one another, wherein the motor head piece is a separate component which can be coupled to the communication units.

Abstract: A single-cell screening device, a screening assembly, a screening method, and use thereof. The single-cell screening device includes a body, a plurality of spray holes, heating portions, and a flow channel. The spray holes are located in the body, the heating portions are located in the spray holes and used for heating a liquid entering the spray holes to generate bubbles so as to spray out cells. The flow channel is located in the body and extends to the spray holes according to a preset path, so as to deliver a cell suspension into the spray holes. The single-cell screening device of the present disclosure has a simple design and is easy to fabricate. Cell printing (spraying) using the screening assembly of the present disclosure has a much higher cell printing speed than conventional methods, allowing for a rapid cell sorting process.

Abstract: The present disclosure provides systems and methods for recycling gas in a reactor. An example of gas-recycling system comprises: a housing, a gas conduit, and a powered propeller. The housing encloses a gas space and a liquid space, wherein the gas space is configured to collect gas within a reactor. The powered propeller comprises a shaft having an upper end and a lower end; and a plurality of radial blades connected to the lower end of the shaft. Upon rotation of the powered propeller, the powered propeller is configured to: generate a suction to cause the collected gas to flow from the gas space to the liquid space through the conduit; cause fluid of the reactor to flow in a direction from the upper end of the shaft to the lower end of the shaft; and mix the liquid and the collected gas proximate the powered propeller.

Abstract: Systems and methods herein generally relate to prolonging viability of bodily tissue, especially an organ, by adjusting pressure as needed to maintain a constant pressure within the organ even during external pressure fluctuations due, for example, to transportation of the organ in an airplane. The systems and methods herein can include an electronic pump that pumps gas into an organ and a mechanical pressure regulator to release gas based on organ pressure.

Abstract: A system and method for molecular delivery in vitro, ex vivo, or in vivo applications using a synergistic effect of corona charge treatment and elevated temperatures. Applications of the invention include cell death and biological particle destruction/inactivation, including, but not limited to viruses and prions.

Abstract: An apparatus and a method for continuously harvesting a biological substance produced by a cultured cell. An apparatus and a method for continuously harvesting a biological substance produced by a cultured cell using 2 sets of filters having different pore sizes.

Abstract: The invention provides an automated system for producing induced pluripotent stem cells (iPSCs) from adult somatic cells. Further, the system is used for producing differentiated adult cells from stem cells.

Abstract: Methods and systems for creating patterns in tissue growth for tissue engineering are disclosed. In one embodiment, a method for arranging biological cells along predetermined patterns using an ultrasound includes: emitting the ultrasound by an ultrasound transducer; transmitting the ultrasound through a holographic lens toward a plurality of cells; and generating a pressure field in the predetermined patterns. The predetermined pattern includes a plurality of mutually parallel transverse planes. The parallel transverse planes are configured to entrap groups of cells of the plurality of cells. The axial pressure gradients within the parallel transverse planes are smaller than a first predetermined threshold. The lateral pressure gradients within the parallel transverse planes are larger than a second predetermined threshold. In response to generating the pressure field, the groups of entrapped cells are aligned within parallel transverse planes.

Abstract: Described herein are biofilm bioreactors for synthesis at the interface between two liquids, and methods of using such bioreactors for the biotransformation of feedstocks into chemical products. Also contemplated is the extraction of such products.

Abstract: A recycling method for the biomass raw material includes: heating an original biomass raw material, and performing biochemical reaction and/or synthesis reaction on the heated biomass raw material according to a preset treatment condition, to obtain a primary recycled product of the biomass raw material; and performing cyclic shear filtration and/or ultrasonic enhanced dispersion on the primary recycled product to turn the primary recycled product into a secondary recycled product, wherein the secondary resource product includes a solid biomass portion and a liquid biomass portion; the solid biomass portion is returned to the biochemical reaction step; and the liquid biomass portion waits for packaging or the liquid biomass portion waits for packaging after being concentrated. A complete set of equipment included in the system is miniaturized and can be widely used in various scenes that require nearby or on-site solutions for biomass raw material recycling.

Abstract: A cassette assembly (1) comprises a cover (100), two cassette halves (200A, 200B) and a slider (300) comprising multiple test chambers (302). The cassette halves (200A, 200B) comprise waste tanks (230) in fluid connection with reservoirs (240), prefilled in one of the cassette halves (200A, 200B) with test agents. The particular design of the cassette halves (200A, 200B) enable forming predefined 5 volumes of liquid to achieve predefined concentrations of the test agents in the reservoirs (240) in one of the cassette halves (200A, 200B) and liquid in the reservoirs (240) in the other of the cassette halves (200A, 200B). Gradients of the test agents can then be established over the multiple test chambers (302).

Abstract: A fluidic system for producing extracellular vesicles from suspended producer cells, including at least one container, a liquid medium contained in the container, suspended producer cells, a liquid medium agitator, a device for controlling the speed of the agitator suitable for the growth of the suspended producer cells, wherein the device for controlling the speed of the agitator, the agitator and the shape and dimensions of the container are suitable for generating a turbulent flow of the liquid medium in the container for exerting shear stresses on the producer cells in order to carry out the production of extracellular vesicles, the Kolmogorov length of the flow being less than or equal to 50 ?m.

Abstract: A centrifugal bioreactor includes a turntable assembly, an electric motor coupled to the turntable assembly, and a replaceable rotor assembly coupled to the turntable assembly. The replaceable rotor assembly includes a plurality of reaction chambers that house cell cultures. A fresh medium tank holds fresh medium for the cell cultures, a spent medium tank holds a first portion of spent medium produced by the cell cultures, and an inoculation tank combines the fresh medium from the fresh medium tank and a second portion of the spent medium. A gas exchanger exposes the fresh medium and the second portion of the spent medium to gas. Additionally, a first pump supplies the fresh medium and the second portion of the spent medium to the plurality of the reaction chambers, and a second pump discard the first portion of the spent medium to the spent medium tank.

Abstract: Some variations provide a multi-stage process for anaerobic digestion of animal manure, comprising: providing a feedstock comprising animal manure; introducing feedstock with a hydrolytic microorganism to a hydrolysis stage; introducing feedstock with an acidogenic microorganism to an acidogenesis stage; introducing feedstock with a methanogenic microorganism to a methanogenesis stage; forming an anaerobic environment in each stage; monitoring the gas composition and the ratio of volatile fatty acids to total alkalinity in each of the hydrolysis stage, the acidogenesis stage, and the methanogenesis stage; and feeding a steady-state amount of the feedstock into the hydrolysis stage, and processing the steady-state amount of the feedstock, continuously or intermittently, through the hydrolysis stage, the acidogenesis stage, and the methanogenesis stage, thereby generating a product comprising biogas.

Abstract: An organic-waste-recycling apparatus for fast formation of compost includes a processing barrel, a grinding device, a control device, a stirring device, and a guiding device. The processing barrel has a first feeding port and a second feeding port, a mixing room, and an output. To-be-processed objects are fed into the mixing room through the first feeding port or the second feeding port. The grinding device is between the first feeding port and the mixing room. The control device adjusts the temperature and the humidity in the mixing room. The stirring device rolls and blends the to-be-processed objects in the mixing room so that the to-be-processed objects are heated evenly. The guiding device guides the water vapor generated in the mixing room back to the mixing room. The apparatus decomposes and turns organic waste into organic carbon fertilizer that has economic value in an effective and efficient way.

Abstract: A flow distribution device for bioprocess systems, comprising: a flow distribution manifold (12; 112) comprising: at least four fluid connection conduits (14), wherein each fluid connection conduit (14) comprises a first end (18) for fluid connection and an opposite second end (20), and wherein at least three of the fluid connection conduits comprise a membrane (19a) and a valve seat (19b), which membrane (19a) can be put in at least two different positions in relation to the valve seat (19b) for allowing or preventing fluid flow between the first end (18) and the second end (20) of the fluid connection conduit (14); and a central common compartment (30) to which the second ends (20) of each of the fluid connection conduits (14) are connected, whereby the first ends (18) of each of the fluid connection conduits (14) can be in fluid communication with the central common compartment (30) and wherein the fluid connection conduits (14) are entering the central common compartment (30) from at least three different

Abstract: This pyrotechnic cell disruption device is provided with: a pyrotechnic charge to be ignited which is configured to be burnt upon ignition; and a pressure chamber for housing a cell-containing fluid sample therein, said pressure chamber being configured to be pressurized when the pyrotechnic charge is ignited and burnt.

Abstract: A germination box having a container for receiving grain. A beam is arranged inside the container. Arranged on the beam is a conveyor device, in particular a worm screw, which is arranged horizontally with respect to a base and serves to load and unload the container, wherein the conveyor device is movable in a vertical direction. A plurality of turning screws, arranged vertically with respect to the base, are mounted on the beam and are operated in pairs in opposite directions in order to turn the grain. Stripping tools are mounted at an end of each of the turning screws facing towards the base. The turning screws are configured to be operated in the same directions in order to empty the germination box. The invention makes available a method for emptying the germination box according to the invention, and a method for retrofitting a germination box.

Abstract: The present invention relates to a novel bioreactor system for preparing an engineered three-dimensional biological tissue construct. The bioreactor system comprises a cultivation chamber that is designed to allow the formation, cultivation and the subsequent testing and/or stimulation of tissue constructs on one or more support elements with a minimum risk of microbial contamination or mechanical damage. The invention furthermore relates to a method for preparing an engineered tissue construct using the novel bioreactor system. The invention also relates to the use of the bioreactor system for preparing engineered biological tissue constructs, preferably tissue constructs which are suitable for being used in clinical tissue replacement and reconstructive therapy, drug development, drug screening, toxicity testing, cosmetic studies, safety testing, developmental studies, disease modeling, or food purposes.