Patents Examined by William H. Beisner
  • Patent number: 11028360
    Abstract: The present invention provides a separator for microorganisms in cavitary contents. The separator for microorganisms in cavitary contents includes a raw material vessel, a stage filter, a liquid storage vessel, a closed separated material vessel, a separate loading unit, a deodorizer, pipelines for connecting all the units, etc.
    Type: Grant
    Filed: February 15, 2015
    Date of Patent: June 8, 2021
    Inventors: Faming Zhang, Youquan Zhao, Huiquan Wang, Bota Cui, Pan Li, Guozhong Ji
  • Patent number: 11028432
    Abstract: Methods, devices, and kits are provided for performing PCR and other thermal cycling reactions in <20 seconds per cycle, using induction heating.
    Type: Grant
    Filed: November 5, 2014
    Date of Patent: June 8, 2021
    Assignees: BioFire Diagnostics, LLC, University of Utah Research Foundation
    Inventors: David Jones, Carl T. Wittwer
  • Patent number: 11014056
    Abstract: Agitating device (10) for a digester (1) of a biogas plant (100) having a housing (11) and a driving device (12) for rotatably driving the agitator blades (13-15). The driving device (12) comprises a drive shaft (16) and an electric drive motor (20) wherein the drive motor (20) is accommodated sealed in the housing (11). The drive motor (20) comprises an outer, hollow stator (21) and a rotary rotor (22) which is centrally accommodated therein and is configured at least partially hollow. The rotor (22) is rotatably supported at the housing (11) and comprises a coupling device (23) for non-rotatable coupling with the drive shaft (16) to drive the at least one agitator blade (13-15) by means of the drive shaft (16).
    Type: Grant
    Filed: November 5, 2015
    Date of Patent: May 25, 2021
    Inventor: Andreas Czwaluk
  • Patent number: 11016031
    Abstract: The present invention relates in one aspect to a ballast water analysis system comprising fluorometer and light scattering meter. The fluorometer comprises a first light source arranged to illuminate a first ballast water sample for obtaining a first fluorescence measurement on a first ballast water sample. The light scattering meter comprises a second light source arranged to illuminate a second ballast water sample with a second light beam and first and second photodetectors arranged to receive light at respective angles relative to a direction of the second light beam. The second and third photodetectors are configured to receive scattered light resulting from interaction between light from the second light source and matter, such as viable or non-viable microorganisms and other particles, in the second ballast water sample.
    Type: Grant
    Filed: November 3, 2015
    Date of Patent: May 25, 2021
    Assignee: Ballast Water Monitoring A/S
    Inventors: Ole Olsen, Kristoffer Kampmann
  • Patent number: 11015162
    Abstract: The present disclosure provides a flow-through electroporation device configured for use in an automated multi-module cell processing environment and configured to decrease cell processing time and the risk of clogging.
    Type: Grant
    Filed: January 2, 2021
    Date of Patent: May 25, 2021
    Assignee: Inscripta, Inc.
    Inventors: Nathan Bade, Jorge Bernate, Phillip Belgrader, Don Masquelier
  • Patent number: 11015159
    Abstract: A single-use cell culturing apparatus and a culturing bag are provided, each of which can improve efficiency of stirring a culture solution and further reduce a possibility of leakage of the culture solution. The single-use cell culturing apparatus of the present invention includes a housing that accommodates the culturing bag which is flexible and can seal the culture solution inside thereof. The housing has, on an inner surface thereof, a curved convex-shaped protrusion provided at a portion of a contact face at which the housing is in contact with the culturing bag. The culturing bag of the present invention is accommodated in the housing of the single-use cell culturing apparatus; is flexible; and can seal the culture solution inside thereof. The culturing bag includes a concave portion having a shape corresponding to the curved convex protrusion disposed on a part of the housing.
    Type: Grant
    Filed: July 1, 2016
    Date of Patent: May 25, 2021
    Inventors: Masaru Nanba, Takashi Andou, Yui Sugita, Sei Murakami, Hiroyuki Matsuda
  • Patent number: 11015160
    Abstract: A filter holding device (1; 41; 75; 81) arranged for providing at least one filter (7; 43a, 43b; 85) inside a flexible bag (3; 71), said filter holding device comprising attaching means (19a-d, 21a-d; 79; 83) for attaching the filter holding device to an inner surface of the flexible bag such that the at least one filter held by the filter holding device is provided at a distance from the inner surface of the flexible bag.
    Type: Grant
    Filed: September 12, 2016
    Date of Patent: May 25, 2021
    Assignee: Cytiva Sweden AB
    Inventors: Eric Faldt, Ralph Stankowski
  • Patent number: 11001798
    Abstract: The present invention provides an apparatus for applying electrical stimulation to cells in a liquid culture medium without using electrodes immersed in the liquid culture medium. This apparatus is an electrical stimulation apparatus A for electrically stimulating cells in a liquid culture medium (2). A liquid culture medium vessel (3) includes: a ring-shaped recess (7) for holding the liquid culture medium (2); and a through hole (6) formed within the ring-shaped recess (7). A magnetic core (1) is made of a magnetic material and disposed such that a portion thereof is inserted through the through hole (6) of the liquid culture medium vessel (3). An excitation coil (5) is wound around the magnetic core (1). A coil power supply (8) supplies a varying current to the excitation coil (5).
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: May 11, 2021
    Inventors: Ryoichi Nagatomi, Toshiyuki Takagi, Shinichi Izumi, Toshihiko Abe, Hitoshi Mori, Kenji Yashima, Kazumi Mori
  • Patent number: 11000031
    Abstract: A method of decellularizing a tissue includes disposing the tissue within a device, the device. The device includes a container, first and second electrodes disposed within the container and defining a space between the first and second electrodes to receive the tissue, a perfusion pump, and a conduit connected to the perfusion pump to transport a decellularization composition from the pump into the tissue. The method further includes disposing the decellularization composition within the container to surround the tissue and contact the first and second electrodes, applying an electric potential across the first and second electrodes, and perfusing the decullarization composition into the tissue.
    Type: Grant
    Filed: May 12, 2018
    Date of Patent: May 11, 2021
    Inventors: Robert C. Chin, Leonid Bunegin
  • Patent number: 11000804
    Abstract: A system for removing undesirable compounds from contaminated air includes a biofilter having porous glass media. Hydrogen sulfide is removed from contaminated air by passing the contaminated air through the biofilter.
    Type: Grant
    Filed: June 10, 2020
    Date of Patent: May 11, 2021
    Assignee: Evoqua Water Technologies LLC
    Inventor: Timothy Frank Matheis
  • Patent number: 11001797
    Abstract: A system and methods for aerosol delivery of an entity or agent are disclosed. The system and methods can include a target application surface. A nebulizer can be located in close proximity to the target application surface. The nebulizer can include a chamber to hold the entity, a nozzle plate including one nozzle, and a piezoelectric element coupled to the nozzle plate. A power source can be coupled to the piezoelectric element. The power source, when activated, can energize the piezoelectric element to vibrate the nozzle plate to cause the entity to be nebulized through the nozzle to impact the target application surface.
    Type: Grant
    Filed: April 15, 2013
    Date of Patent: May 11, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Karel Domansky, Michael Karpelson, Donald E. Ingber
  • Patent number: 10987670
    Abstract: A device includes a microfluidic trap disposed along a microfluidic channel, the trap and channel having dimensions to create a fluid vortex within the trap to trap a particle of interest and an electrode having interdigitated electrically isolated fingers positioned in the trap to create an electric field across the trap such that the electric field causes electroporation of a molecule into the particle of interest. A further device includes an array of channels, traps and interdigitated electrically isolated fingers.
    Type: Grant
    Filed: April 14, 2016
    Date of Patent: April 27, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Soojung Claire Hur, Mengxing Ouyang
  • Patent number: 10988726
    Abstract: Systems and methods for automated cell culturing are disclosed. In some embodiments, one or more cell culture vessels are fluidly connected with one or more multiport valves and one or more fluid pumps. The fluid pumps may pump various fluids into and out of the cell culture vessels as necessary to support cell growth, routed by the one or more multiport valves. In some embodiments, one or more components may be removable from other components so that some components may be prepared and sterilized independently prior to usage.
    Type: Grant
    Filed: August 16, 2019
    Date of Patent: April 27, 2021
    Inventors: Ali Afshar, James Cunningham, Henry Miskin, Ignacio Willats
  • Patent number: 10988724
    Abstract: The present disclosure relates to the design, fabrication, and applications of a three-dimensional (3D) bioreactor for cell expansion and cell secreted substance production. The bioreactor is composed of non-random interconnected voids providing a continuous three-dimensional surface area for cell adherence and growth.
    Type: Grant
    Filed: May 3, 2017
    Date of Patent: April 27, 2021
    Assignee: Southwest Research Institute
    Inventors: Jian Ling, Jeffrey N. Harris, Michael J. Rubal
  • Patent number: 10988723
    Abstract: The present invention relates to modular assemblies and systems for conducting cell analyses. In particular embodiments, an assembly has micron-scale fluidic structures, thereby minimizing sample use, and possesses a modular architecture that facilitates disassembly of the apparatus. Thus, one such modular assembly facilitates on-chip cell growth and on-chip assays; and then, as needed, the assembly can be disassembled to provide direct access to the cells that were incubated and treated within the chip. Methods of making and using such assemblies, as well as systems thereof, are further described herein.
    Type: Grant
    Filed: September 22, 2016
    Date of Patent: April 27, 2021
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Anson Hatch, Vinay Abhyankar, Chung-Yan Koh
  • Patent number: 10983031
    Abstract: A method for producing a sample slice includes providing a disk, providing a jig on the disk for supporting side portions of a material, providing the material inside the jig, providing a pillar assembly on the jig, the pillar assembly that includes a first base member having a plurality of through holes, a second base member detachably mounted to said first base member, and a plurality of pillars supported between said first and second base members and arranged to be exposed through the plurality of holes, inserting the plurality of pillars into the material, solidifying the material, separating the jig and the pillar assembly to remove the material from the jig, and cutting a sample slice from the material with a cutter.
    Type: Grant
    Filed: October 15, 2019
    Date of Patent: April 20, 2021
    Inventors: Hyo Jeong Kuh, Ji Hoon Kang, Dong Wo Lee
  • Patent number: 10982180
    Abstract: Provided is a system and a method for propagating cells, the system comprising a multi well plate and a plate sealing means for sealing at least one well of a multiwell plate, the plate sealing means comprising at least one solid bulge or at least one bulge comprising a solid base, said at least one bulge consisting of a resilient elastomer to securely seal at least one well of the multiwell plate. Also disclosed is the use of such a system for propagating cells and the corresponding method.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: April 20, 2021
    Assignee: INSPHERO AG
    Inventors: Bjoern Niggemann, Jan Lichtenberg, Wolfgang Moritz, Jens Kelm
  • Patent number: 10982182
    Abstract: The present invention discloses a flow electroporation device comprising a three-dimensional electrode device and a flow electroporation chamber, the three-dimensional electrode device comprising an electrode array and an electrode fixing assembly for fixing electrodes in the electrode array, the flow electroporation chamber comprising a cavity, an inlet, an outlet and an opening, the inlet, the outlet and the opening on the cavity, and the electrode array being inserted into the cavity through the opening. By means of the above-mentioned manner, the flow electroporation device of the present invention can achieve a high throughput cell treatment, and also can work under a low voltage condition, can avoid damages to cells brought by liquid flow, can reduce effects brought by heat and cathode effect induced by electric pulses, and can maintain an closed aseptic environment.
    Type: Grant
    Filed: June 19, 2020
    Date of Patent: April 20, 2021
    Assignee: ETTA BIOTECH CO., LTD.
    Inventor: Edward Dai
  • Patent number: 10981112
    Abstract: The present disclosure relates to a filter material which can be used in reducing the content of contaminants in a raw gas or liquid and a device which comprises the filter material. The disclosure also relates to a method for reducing the content of contaminants in a raw gas or liquid which applies the filter material and/or device.
    Type: Grant
    Filed: April 26, 2017
    Date of Patent: April 20, 2021
    Inventor: Bonno Koers
  • Patent number: 10975345
    Abstract: A compact culture device capable of performing large-scale culture in an aseptic state. A culture device has a culture bag which has ports through which a liquid flows and enables cell culture, a bag holding portion having holding plates holding the culture bag and rotating shafts, a rotation mechanism supporting and rotating the rotating shaft, a liquid supply/discharge mechanism supplying and discharging a liquid to/from the culture bag, and a rotation control portion controlling the rotation of the rotation mechanism.
    Type: Grant
    Filed: May 24, 2016
    Date of Patent: April 13, 2021
    Inventors: Masakatsu Takeuchi, Atsushi Taguchi