Abstract: An automated cell culture and tissue engineering system comprising defined and separate environmental zones provide for increased control and maintenance of the internal environment of the system such that the temperature, air flow and gases surrounding the bioreactor module form one zone that is maintained separately to a second zone formed surrounding the reagent fluid reservoir. The system further comprises means for elimination and/or management of condensation within the second zone of the system.

Abstract: The invention features devices and kits for capturing and culturing microorganisms (e.g., bacteria, fungi, or protists) and methods of using the devices and kits to detect microorganisms in environmental and other samples. The device includes a nutrient media having a flat growth area on which microorganisms can grow. Samples are collected by contacting the device with any environmental sample, e.g., rolling device on a work surface or exposing device to air, or by filtering a sample through a membrane. Microorganisms deposited on the membrane derive nutrients from the underlying media and grow into colonies that can then be detected using methods known in the art. The detected colonies can be imaged digitally or with film.

Abstract: The device for a cell suspension culture comprises a culture chamber comprising first and second axisymmetric-shaped walls having at least a partially curved radial section the concavity thereof facing the inside of the culture chamber, the walls delimiting an inner compartment and form an inner continuous surface; an inlet opening provided in the first wall and adapted to enable a fluid to enter; a supplying conduit connected to the inlet opening; and a valve member disposed inside the culture chamber adapted to tightly close or open the inlet opening. The device comprises also a device for moving the valve member, disposed outside the culture chamber.

Abstract: A growth chamber system includes a power source and an inner chamber. An access opening is included to the inner chamber. An outer chamber surrounds at least one wall of the inner chamber. A temperature sensor is included which monitors the temperature inside the inner chamber. A heater and pump are fluidly connected to the outer chamber and a working fluid is circulated by the pump through the heater and outer chamber such that an increase in temperature of the working fluid produces a temperature increase within the inner chamber by contact of the working fluid with an outer surface with at least one wall of the inner chamber. A rotor blade is positioned within the inner chamber and connected to an electric motor. At least one controller is included and connected to the fluid heater, pump, and electric motor and configured to control temperature and rotation speed.

Abstract: Disclosed are devices and methods for non-cryogenic vitrification of biological materials that include the steps of providing one or more capillary channels of which a first opening is operably in contact with a moisture containing vitrification mixture made of a biological material and a vitrification agent.

Abstract: An apparatus for perfusing an organ or tissue includes a perfusion circuit for perfusing the organ or tissue; a controller configured to control the apparatus; and a detector configured to detect an optional component of the apparatus, and provide a signal to the controller indicative of the presence of the optional component, wherein the controller is configured to control the perfusion circuit in a first mode when the optional component is detected and to control the perfusion circuit in a second mode when the optional component is not detected.

Abstract: A method includes simultaneously measuring electrophysiological responses and contractility responses of a plurality of cells forming a tissue culture using a system comprising a sensor array configured to simultaneously measure the electrophysiological responses and the contractility responses of the plurality of cells forming the tissue culture. Fabrication techniques for making such systems include: forming a sensor array comprising an interpenetrating arrangement of IDEs and electrodes of a MEA in or on a substrate surface; forming a plurality of contacts for interfacing the system with one or more external devices in or on the substrate surface; and forming leads between the plurality of contacts and the sensor array.

Abstract: A fermentation device includes a fermenter. The fermenter includes a tank, augers, and a motor. The tank is horizontally arranged. A first end of the tank is connected with a material feeding port. A second end of the tank is connected with a material discharging port. A height of the material discharging port is not less than a height of the tank. Liquid raw material and solid raw material are added into the tank from the material feeding port. The augers are arranged in the tank. Each of the augers includes a central shaft and a blade. An inner edge of each blade is connected with a corresponding central shaft. A gap is defined between an outer edge of each blade and inner walls of the tank. The motor is connected with the augers and drives the augers to rotate in a first direction or a second direction.

Abstract: The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.

Abstract: A photobioreactor for cultivation and/or propagation of a photosynthetic organism and associated systems/methods are disclosed herewith. The photobioreactor includes (1) a substantially spherical vessel having a wall defining an interior vessel volume; (2) a water-submersible system for converting electrical energy into electromagnetic radiation; (3) a temperature management system for circulating heat dispersal fluid into and out of the water-submersible system; and (4) a photobioreactor control system comprising a processor and a controller.

Abstract: The disclosure provides for multilayered organ-on-a-chip systems that can be used to generate topographic neural organoids, and uses thereof, including as models to study neurological disorders.

Abstract: An array platform for three-dimensional cell culturing and drug testing and screening is disclosed. In the array platform, a hydrogel-cell mixture injection area is configured to inject a plurality of kinds of hydrogel-cell mixtures. Cell observation areas are connected to the hydrogel-cell mixture injection area. Electrodes are disposed under the cell observation areas and automatic cell quantification and three-dimensional cell co-arrangement of the plurality of kinds of hydrogel-cell mixtures in the cell observation areas through the electrodes to imitate a structure of body's tissues. A drug injection area is configured to inject a plurality of kinds of drugs. Drug combination generators respectively correspond to the cell observation areas and are connected to the drug injection area. Each drug combination generator has a microfluidic channel structure and configured to generate drug combinations according to the plurality of kinds of drugs.

Abstract: It is solved by the rod-shaped tubular cell structure maintaining support device for cultivating a tubular cell structure including a hollow portion inside for a predetermined period of time while maintaining the hollow portion, the tubular cell structure maintaining support device including an outer diameter insertable into the hollow portion of the cell structure, and when inserted into the hollow portion, capable of allowing the tubular cell structure maintaining support device to adhere to an inner surface of the cell structure, and a penetration conduit including openings at both ends of the tubular cell structure maintaining support device, and penetrating between the openings, wherein the tubular cell structure maintaining support device has an oxygen permeable structure from the penetration conduit to an outer surface of the tubular cell structure maintaining support device.

Abstract: The gas collection device includes a constant temperature chamber whose interior is maintained at a set temperature; a culture flask unit located inside the constant temperature chamber and culturing bacteria therein; a mass flow controller located outside the constant temperature chamber and connected to the culture flask unit through an injection flow path to control a gas injected into the culture flask unit through the injection flow path; and an impinger located outside the constant temperature chamber and connected to the culture flask unit through a discharge flow path to receive a gas discharged from the culture flask unit through the discharge flow path in real time.

Abstract: The disclosure relates to systems, devices and methods for at least one of cell and gene therapy manufacture. In some embodiments, a cell processing unit is provided and comprises a housing defining an enclosure into which a cell processing platform can be mounted, a platform mounting bracket within the housing and configured and arranged to receive and retain a cell processing platform, a drive apparatus configured and arranged to operatively engage and act upon a cell processing platform so as to move same with respect to the platform mounting bracket, and an actuator configured and arranged to exert a force on a container mounted into the cell processing platform so as to expel a contents from the container.

Abstract: A system, device and method for electroporation of living cells and the introduction of selected molecules into the cells utilizes a fluidic system where living cells and biologically active molecules flow through a channel that exposes them to electric fields, causing the molecules to be transferred across the cell membrane. The device is structured in a manner that allows precise control of the cells location, motion, and exposure to electric fields within the flow channel device. The method is particularly well suited for the introduction of DNA, RNA, drug compounds, and other biologically active molecules into living cells.

Abstract: The present invention relates to bioreactors for root organ cultures that enable large parts of the inoculation or biomass transfer, cultivation and harvest of the cultures to be performed outside of an aseptic environment. The present invention further relates to methods of producing and aseptically harvesting roots, and methods of producing root cultures using the aforementioned bioreactors alone or as part of an apparatus further comprising an inoculation vessel or at least one further bioreactor.

Abstract: There is disclosed a cell culture plate comprising at least two sequentially arranged wells and a channel adapted for fluid communication between the wells, wherein the channel is connected or coupled to a first pump via openings at each end of the channel, and wherein said first pump is operable to circulate fluid between the at least two wells. Devices and systems incorporating the cell culture plate and methods and uses employing same are also disclosed.

Abstract: The present invention relates to a blood diagnostic device, which includes one or more blood input parts into which blood is injected, a deterministic lateral displacement separation part which communicates with the blood input part to form a blood flow path along which the blood flows and separates white blood cells from remaining blood components, a first microchannel which communicates with the deterministic lateral displacement separation part so that the white blood cells separated through the deterministic lateral displacement separation part flow therein, one or more second microchannels which communicate with the deterministic lateral displacement separation part so that the remaining blood components separated through the deterministic lateral displacement separation part flow therein, a first discharge part which communicates with the first microchannel so that the white blood cells flowing in the first microchannel are discharged therethrough, and a second discharge part which communicates with the

Abstract: The disclosure provides, in various embodiments, systems, devices and methods relating to ex-vivo organ care. In certain embodiments, the disclosure relates to maintaining an organ ex-vivo at near-physiologic conditions. The present application describes, for example, a method for using lactate measurement in the arterial and the venous blood lines of the Organ Care System Heart perfusion device to evaluate, for example, the: 1) overall perfusion status of an isolated heart; 2) metabolic status of an isolated heart; and 3) overall vascular patency of an isolated donor heart. This aspect of the present disclosure may use, for example, the property of myocardial cell's unique ability to produce/generate lactate when they are starved for oxygen and metabolize/utilize lactate for energy production when they are well perfused with oxygen.