Abstract: Described herein is a multiplex 5mC marker barcode counting (MMBC) to quantify 5mC markers in DNA, in which multiple 5mC markers could be targeted for capture and linear amplification. The inventors' method provides highly sensitive and specific quantification of multiple 5mC loci.

Abstract: The present disclosure provides a method and a vehicle control system for controlling an autonomous vehicle. The method comprises utilizing the sensor group of said vehicle to acquire data related to said vehicle, wherein said data includes the speed of said vehicle, the speed of another object ahead of said vehicle, and the distance between said vehicle and another object, determining the crash probability between said vehicle and another object according to the data related to said vehicle, calculating the total magnitude of value according to said crash probability on the basis of the prospect theory, wherein said total magnitude of value indicates the balance between the value of crash risk reduction and the value of user comfort loss, and calculating the brake force according to said total magnitude of value.

Abstract: A spray-dried zirconocene catalyst system comprising a zirconocene catalyst and a hydrophobic fumed silica, which supports the zirconocene catalyst. A spray-drying method of making same. Polyolefins; methods of making and using same; and articles containing same.

Abstract: A vehicle includes a traction battery. The vehicle further includes a controller programmed to generate a capacity estimate of the traction battery and, in response to an amount of energy supplied to the traction battery during a charge cycle being less than an estimated amount of energy stored in the traction battery during the charge cycle, detect that the capacity of the traction battery has changed and alter an operating strategy of the traction battery until the capacity estimate is updated.

Abstract: The present invention relates to methods for expanding a stem cell population, as well as other cell populations. More particularly, the invention relates, inter alia, to methods and compositions for expanding a stem cell and/or other cell population, particularly a hematopoietic stem cell population.

Abstract: A vehicle includes a traction battery and a controller in communication with the battery and programmed to control battery charging in response to a user-selected one of a plurality of charging strategies having different charging rates based on detection of lithium plating in the battery. The charging strategies may include options for faster charging with an urgent or emergency charging strategy selectable a limited number of times to mitigate battery performance degradation associated with lithium plating. A method implemented by a vehicle controller in a vehicle having a traction battery, may include controlling, by the controller, battery charging in response to a user-selected charging strategy selected from one of a plurality of available charging strategies each having a different charging rate and displayed on a user interface in response to detection of lithium plating in the traction battery, at least one charging strategy associated with additional lithium plating if selected.

Abstract: Lithium ion battery state of charge (SOC) is a function of open circuit voltage (OCV). Battery internal diffusion process needs to be nearly completed to be able to measure battery open circuit voltage. The length of the minimum settling time depends on the battery type, usage and temperature. Described are methods to determine electric vehicle battery voltage relaxation time based on battery temperature and usage history.

Abstract: A vehicle includes a battery pack, an electric motor, and a contactor to electrically connect the pack and motor. The contactor is configured with a control circuit to electrically connect the pack and motor. The control circuit includes a leak detection sensor. The vehicle further includes a controller to output a leakage resistance associated with the pack. The leakage resistance is based on a voltage of the pack and a leak voltage detected by the sensor while the contactor is closed.

Abstract: The current disclosure provides a method that can specifically label and directly amplify 5hmC site on genomic DNA without pull-down or bisulfite treatment, which enables one to map the 5hmC site from a single DNA molecule. Aspects of the disclosure relate to a method for detecting 5-hydroxymethylcytosine (5hmC) nucleic acid bases in a nucleic acid molecule or a plurality of nucleic acid molecules, the method comprising: a. modifying the 5hmC nucleic acid base with a first functional group; b. covalently attaching a modified nucleic acid probe comprising a second functional group to the first functional group; wherein the nucleic acid probe and nucleic acid molecule are covalently linked through the first and second functional groups; c. annealing a primer to the nucleic acid probe; d. performing primer extension of the annealed primer to make a new strand; and e. detecting the new strand.

Abstract: A system and method to verify software includes a debugger setting a breakpoint in the software. The breakpoint indicates a point at which to pause or stop execution of the software. The method also includes setting one or more anchor points associated with the breakpoint. Each of the one or more anchor points represents another point in the software that must be executed prior to pausing or stopping the execution of the software at the breakpoint.

Abstract: The present invention relates generally to the field of molecular biology. More particularly, it concerns methods and compositions for detecting, evaluating, and/or mapping 5-hydroxymethyl-modified cytosine bases within a nucleic acid molecule.

Abstract: A system and method to verify software includes a debugger setting a breakpoint in the software. The breakpoint indicates a point at which to pause or stop execution of the software. The method also includes setting one or more anchor points associated with the breakpoint. Each of the one or more anchor points represents another point in the software that must be executed prior to pausing or stopping the execution of the software at the breakpoint.

Abstract: Lithium ion battery state of charge (SOC) is a function of open circuit voltage (OCV). Battery internal diffusion process needs to be nearly completed to be able to measure battery open circuit voltage. The length of the minimum settling time depends on the battery type, usage and temperature. Described are methods to determine electric vehicle battery voltage relaxation time based on battery temperature and usage history.

Abstract: Methods, compositions and kits are provided to amplify the amount of genomic methylated DNA can by subsequently analyzed and/or sequenced. It has particular use with small amounts of DNA, including, but not limited to cell free DNA samples. In some embodiments, the ratio of polymerase and methyltransferase is controlled in order to provide maximum yields. In some embodiments, a dual primase/polymerase is used.

Abstract: A vehicle includes a traction battery having cells and at least one controller. The controller is programmed to charge and discharge the traction battery according to a net current for the traction battery derived from sets of temperature-based current values, each of the sets corresponding to a different one of the cells, and each of the values for a given one of the sets corresponding to a different zone of the cell.

Abstract: A system and method to verify software includes a debugger setting a breakpoint in the software. The breakpoint indicates a point at which to pause or stop execution of the software. The method also includes setting one or more anchor points associated with the breakpoint. Each of the one or more anchor points represents another point in the software that must be executed prior to pausing or stopping the execution of the software at the breakpoint.

Abstract: A hybrid electric vehicle (HEV) and method of operation includes a thermal management system (TMS) that maintains optimal battery temperature operating ranges. The TMS includes a controller, heater, pump, and valves that control a flow rate of a convective fluid about the battery and other components. The TMS responds to battery temperature and differential signals, converting them into pump speed and heater signals utilizing a fuzzy logic multidimensional membership function (MF) that improves performance while reducing energy consumption by generating real-time, smoothed pulse width modulated (PWM) pump speed and heater signals. The MF capability requires less processing power than prior systems, which in turn enables improved response time to battery thermal and temperature rate changes during HEV operation.

Abstract: Lithium ion battery state of charge (SOC) is a function of open circuit voltage (OCV). Battery internal diffusion process needs to be nearly completed to be able to measure battery open circuit voltage. The length of the minimum settling time depends on the battery type, usage and temperature. Described are methods to determine electric vehicle battery voltage relaxation time based on battery temperature and usage history.

Abstract: A system and method to verify software includes a debugger setting a breakpoint in the software. The breakpoint indicates a point at which to pause or stop execution of the software. The method also includes setting one or more anchor points associated with the breakpoint. Each of the one or more anchor points represents another point in the software that must be executed prior to pausing or stopping the execution of the software at the breakpoint.

Abstract: A computer-implemented method includes analyzing memory content a first time, where the memory content results from running a program. Based on the analyzing the memory content the first time, first description data is generated describing a plurality of instances of a plurality of known data structures defined in the program. A request is received for a plurality of instances of a new data structure, where the new data structure is not defined in the program. A definition of the new data structure is received separate from the program. Based on the analyzing the memory content a second time, second description data is generated describing a plurality of instances of the new data structure.