Abstract: The invention provides compositions and methods for reducing expression of a target gene in a cell, involving contacting a cell with an isolated double stranded nucleic acid (dsNA) in an amount effective to reduce expression of a target gene in a cell. The dsNAs of the invention possess a single stranded extension (in most embodiments, the single stranded extension comprises at least one modified nucleotide and/or phosphate back bone modification). Such single stranded extended Dicer-substrate siRNAs (DsiRNAs) were demonstrated to be effective RNA inhibitory agents compared to corresponding double stranded DsiRNAs.

Abstract: Apparatus and methods are provided to minimize waste and improve quality and production in web processing operations. The apparatus and methods provide defect detection in deposition of acquisition material, which on current machines frequently flips and is difficult to detect when it has flipped causing manufacturers to scrap thousands of products. Using the present invention, defects are able to be detected by discerning a difference in the appearance from side to side with a vision camera, and an acquisition inverter can flip the material to a correct orientation.

Abstract: The present invention relates to lipid compounds and uses thereof. In particular, the compounds include a class of cationic lipids having an amine moiety, such as an amino-amine or an amino-amide moiety. The lipid compounds are useful for in vivo or in vitro delivery of one or more agents (e.g., a polyanionic payload or an antisense payload, such as an RNAi agent).

Abstract: The invention provides compositions and methods for reducing expression of a target gene in a cell, involving contacting a cell with an isolated double stranded nucleic acid (dsNA) in an amount effective to reduce expression of a target gene in a cell. The dsNAs of the invention possess a single stranded extension (in most embodiments, the single stranded extension comprises at least one modified nucleotide and/or phosphate back bone modification). Such single stranded extended Dicer-substrate siRNAs (DsiRNAs) were demonstrated to be effective RNA inhibitory agents compared to corresponding double stranded DsiRNAs.

Abstract: A device and method for locally authenticating an accessor device to access an operable device. The method comprises receiving reservation information at a validation device from an access granting device, the reservation information identifying one or more accessor devices as having permission to utilize the operable device, where the operable device is local to (e.g., within a predetermined proximity) of the validation device. The method further comprises receiving a request from an accessor device requesting permission to utilize the operable device, the request including an identifier of the requesting accessor device, validating the request based on the reservation information and the identifier, and when validation is successful, granting to the requesting accessor device permission to utilize the operable device.

Abstract: There is disclosed a dispenser (10), such as a confectionery dispenser, for dispensing product objects such as pieces of confectionery (40). The dispenser (10) comprises a container (12) for storing product objects; and at least one dispensing arrangement (14, 16). The dispensing arrangement (14), (16) comprises an opening (18) provided in the container (12); a collection region (26) located within the container (12) and adjacent to the opening (18); a guide (32) disposed within the container (12) and adjacent to the collection region (26); and a dispensing tray (29) disposed within the container (12) and adjacent to the opening (18). The dispensing arrangement (14), (16) is configured the opening (18) and having a dispensing surface 30 accessible through such that in use in a loading orientation of the dispensing arrangement (14), (16) product objects collect in the collection region (26).

Abstract: A school bus stop arm and drive unit therefor are disclosed. The stop an includes a stop sign and a drive unit for moving the stop sign between a retracted position and a deployed position. The drive unit comprises a motor operably coupled to the stop sign, one or more position sensors operable to detect the presence of the stop sign, and a controller for controlling operation of the motor. The controller configured to, monitor one or more operating characteristics of the motor for conditions indicative of an obstruction to movement of the stop sign, monitor the position sensor for a confirmation signal indicative of the presence of the stop sign in the deployed or retracted position, and based on the conditions indicative of an obstruction and the confirmation signal, determine if the stop sign has reached one of the deployed and retracted positions or is being obstructed.

Abstract: The present invention relates to lipid compounds and uses thereof. In particular, the compounds include a class of cationic lipids having an amine moiety, such as an amino-amine or an amino-amide moiety. The lipid compounds are useful for in vivo or in vitro delivery of one or more agents (e.g., a polyanionic payload or an antisense payload, such as an RNAi agent).

Abstract: A device and method for locally authenticating an accessor device to access an operable device. The method comprises receiving reservation information at a validation device from an access granting device, the reservation information identifying one or more accessor devices as having permission to utilize the operable device, where the operable device is local to (e.g., within a predetermined proximity) of the validation device. The method further comprises receiving a request from an accessor device requesting permission to utilize the operable device, the request including an identifier of the requesting accessor device, validating the request based on the reservation information and the identifier, and when validation is successful, granting to the requesting accessor device permission to utilize the operable device.

Abstract: Apparatus and methods are provided to minimize waste and improve quality and production in web processing operations. The apparatus and methods provide defect detection in deposition of acquisition material, which on current machines frequently flips and is difficult to detect when it has flipped causing manufacturers to scrap thousands of products. Using the present invention, defects are able to be detected by discerning a difference in the appearance from side to side with a vision camera, and an acquisition inverter can flip the material to a correct orientation.

Abstract: Techniques for transmitting messages to send configuration information, and/or commands, to features, agents, applications and/or other aspects of endpoints and/or networked computing devices. In an example, server, a host of an analytics platform, or other computing device may send messages within a network to an endpoint that is remote from the computing device. In the example, a first message may be sent to a first agent operating on the endpoint, wherein the first message directs configuration of a first feature of the first agent. The computing device may receive consumption data from the endpoint, which may be monitored and/or analyzed by the analytics platform of the computing device. In the example, a second message may be sent to a second agent operating on the endpoint, wherein the second message has a parameter that allows free-format data, and wherein a command is encoded within the parameter.

Abstract: The description relates to devices and device adjustment. One example can include a first portion that defines a cam pivot. The example can also include a height adjuster comprising a wedge and a positioning loop positioned over the cam pivot, such that rotation of a cam on the cam pivot to contact one cam follower of the positioning loop that moves the wedge toward another component or to contact an opposite cam follower of the positioning loop that moves the wedge away from the another component.

Abstract: The invention provides compositions and methods for reducing expression of a target gene in a cell, involving contacting a cell with an isolated double stranded nucleic acid (dsNA) in an amount effective to reduce expression of a target gene in a cell. The dsNAs of the invention possess a single stranded extension (in most embodiments, the single stranded extension comprises at least one modified nucleotide and/or phosphate back bone modification). Such single stranded extended Dicer-substrate siRNAs (DsiRNAs) were demonstrated to be effective RNA inhibitory agents compared to corresponding double stranded DsiRNAs.

Abstract: The invention provides compositions and methods for reducing expression of a target gene in a cell, involving contacting a cell with an isolated double stranded nucleic acid (dsNA) in an amount effective to reduce expression of a target gene in a cell. The dsNAs of the invention possess a single stranded extension (in most embodiments, the single stranded extension comprises at least one modified nucleotide and/or phosphate back bone modification). Such single stranded extended Dicer-substrate siRNAs (DsiRNAs) were demonstrated to be effective RNA inhibitory agents compared to corresponding double stranded DsiRNAs.

Abstract: The invention provides compositions and methods for reducing expression of a target gene in a cell, involving contacting a cell with an isolated double stranded nucleic acid (dsNA) in an amount effective to reduce expression of a target gene in a cell. The dsNAs of the invention possess a single stranded extension (in most embodiments, the single stranded extension comprises at least one modified nucleotide and/or phosphate back bone modification). Such single stranded extended Dicer-substrate siRNAs (DsiRNAs) were demonstrated to be effective RNA inhibitory agents compared to corresponding double stranded DsiRNAs.

Abstract: A system and method for automatically flushing urine from a pet litter box are disclosed. In one embodiment, the system and method may be directed to a combination of a urine-separating pet litter box with odor controlling conditionally non-absorbent litter and an automatic flushing device. The urine-separating pet litter box includes an upper litter tray with a perforated bottom and a lower liquid holding tank, which holds water inside. The lower liquid holding tank is connected to water intake pipes and a drainpipe and flushes the urine and holding water and fills freshwater in the lower liquid holding tank by detecting a water level of the holding water in the lower liquid holding tank with sensors. The flushing was performed by using a Venturi pump effect to control two electrical valves open or close. In some embodiment, Sodium Sesquicarbonate (SSC) may be used as conditionally non-absorbent litter for complete disposal of feces.

Abstract: The invention provides for double stranded nucleic acid molecules comprising a 5? extension of the sense or antisense strand and further comprising a plurality of nucleotides that are conjugated to a ligand and methods of using the double-stranded nucleic acid molecules. Ligand-modified oligomers where the sense stands form a tetraloop provide new potent and stable RNA interference agents. These dsNA molecules are synthesized using a plurality of nucleotides that include ligand-modified monomers, nucleotide analog monomers, modified nucleotide monomers and the like, using standard nucleotide synthetic methods and systems.

Abstract: There is disclosed a dispenser (10), such as a confectionery dispenser, for dispensing product objects such as pieces of confectionery (40). The dispenser (10) comprises a container (12) for storing product objects; and at least one dispensing arrangement (14, 16). The dispensing arrangement (14), (16) comprises an opening (18) provided in the container (12); a collection region (26) located within the container (12) and adjacent to the opening (18); a guide (32) disposed within the container (12) and adjacent to the collection region (26); and a dispensing tray (29) disposed within the container (12) and adjacent to the opening (18). The dispensing arrangement (14), (16) is configured the opening (18) and having a dispensing surface 30 accessible through such that in use in a loading orientation of the dispensing arrangement (14), (16) product objects collect in the collection region (26).

Abstract: There is disclosed a dispenser 10 for dispensing product objects, comprising: a main chamber 20 for storing product objects; and a portion dispensing mechanism for dispensing a portion comprising a plurality of project objects 40. The portion dispensing mechanism comprises a gate arrangement 36 and a collection chamber 24 which are configured for relative linear movement between a loading configuration and a dispensing configuration. In the loading configuration a plurality of product objects 40 within the main chamber 20 collect within the collection chamber 24 and the gate arrangement 36 prevents product objects 40 from being dispensed from the collection chamber 24. In the dispensing configuration the gate arrangement 36 prevents product objects within the main chamber 20 from entering the collection chamber 24 and a plurality of product objects are dispensed from the collection chamber.

Abstract: Apparatus and methods are provided to minimize waste and improve quality and production in web processing operations. The apparatus and methods provide defect detection both before and after application of component patches to a traveling web to create a product. Web defect detection may be provided by way of at least one visual inspection station located upstream from the patch applicator. Patch defect detection may be accomplished by way of a visual inspection station located proximate the patch applicator. If defects are detected in either the traveling web or the component patch prior to patch application, patch application may be prevented until both a satisfactory web and patch are provided. If defects are detected after patch application, the resulting product may be culled. Furthermore, the apparatus may be provided with diagnostic software to warn against extant or imminent machine complications.