Abstract: The present invention is based, at least in part, upon discovery of a process for identifying phase changing peptides. Such phase changing peptides are capable of enhancing in vitro and in vivo delivery of oligonucleotides (e.g., dsRNAs) in lipidic, vesicular, micellar and/or naked oligonucleotide formulations.

Abstract: A system includes a router transceiver unit that is configured to be disposed on-board a vehicle system. The vehicle system may have at least a source vehicle and a separate linked vehicle that are communicatively linked with each other through a system network of the vehicle system. The router transceiver unit is configured to be communicatively coupled to a requesting operational component of the source vehicle and the system network. The router transceiver unit is also configured to receive a local data packet from the requesting operational component that is directed toward a target operational component of the linked vehicle. The router transceiver unit includes an encapsulation module that is configured to transform the local data packet into an in-tunnel data packet, wherein the local and in-tunnel data packets have different packet formats.

Abstract: Formulations comprising anionic agents such as nucleic acids within a lipid-containing particle methods of formulating a lipid-containing particle comprising an anionic agent such as a nucleic acid, methods for preparing a lipid-containing particle comprising an anionic agent such as a nucleic acid, methods for therapeutic delivery of an anionic agent to a patient in need thereof, where the anionic agent is formulated in a lipid-containing particle as described herein.

Abstract: The present invention is based, at least in part, upon discovery of a process for identifying phase changing peptides. Such phase changing peptides are capable of enhancing in vitro and in vivo delivery of oligonucleotides (e.g., dsRNAs) in lipidic, vesicular, micellar and/or naked oligonucleotide formulations.

Abstract: This invention relates to compounds, compositions, and methods useful for reducing HIF-1? target RNA and protein levels via use of dsRNAs, e.g., Dicer substrate siRNA (DsiRNA) agents.

Abstract: There is provided a voltage or current sensing device. An exemplary voltage or current sensing device includes a drive circuitry configured to deliver a drive current to a magnetic core operably coupled with a conductor, for driving the core to cyclical magnetic saturation. The device also includes sense circuitry configured to receive a voltage signal corresponding to an application current in the conductor. The device also includes signal processing circuitry configured to sample the voltage signal, wherein a first sample is in phase with the drive current and a second sample is out of phase with the drive current. The device also includes a feedback loop configured to deliver a compensation current to the magnetic core, wherein the compensation current is configured to balance the magnetic core and wherein the compensation current is based at least in part on the first sample in phase with the drive current.

Abstract: A system includes a router transceiver unit that is configured to be disposed on-board a vehicle system. The vehicle system may have at least a source vehicle and a separate linked vehicle that are communicatively linked with each other through a system network of the vehicle system. The router transceiver unit is configured to be communicatively coupled to a requesting operational component of the source vehicle and the system network. The router transceiver unit is also configured to receive a local data packet from the requesting operational component that is directed toward a target operational component of the linked vehicle. The router transceiver unit includes an encapsulation module that is configured to transform the local data packet into an in-tunnel data packet, wherein the local and in-tunnel data packets have different packet formats.

Abstract: A communication system is disclosed for use with a train consist. The consist communication system may have a first fluid conduit, a first coupling connected to the first fluid conduit, a first cover connected to the first coupling and configured to close an end of the first fluid conduit, and at least a first cable disposed within the first fluid conduit. The system may also have a second fluid conduit substantially identical to the first fluid conduit, a second coupling substantially identical to the first coupling, fixedly connected to the second fluid conduit, and connectable to the first coupling, and a second cover connected to the second coupling and configured to close an end of the second fluid conduit. At least a second cable may be disposed within the second fluid conduit in communication with the at least a first cable through the first and second covers. The first and second fluid conduits may be pressurized.

Abstract: The invention provides compositions and methods for reducing expression of a target gene in a cell, involving contacting a cell with an isolated double stranded nucleic acid (dsNA) in an amount effective to reduce expression of a target gene in a cell. The dsNAs of the invention possess a single stranded extension (in most embodiments, the single stranded extension comprises at least one modified nucleotide and/or phosphate back bone modification). Such single stranded extended Dicer-substrate siRNAs (DsiRNAs) were demonstrated to be effective RNA inhibitory agents compared to corresponding double stranded DsiRNAs.

Abstract: The invention provides compositions and methods for reducing expression of a target gene in a cell, involving contacting a cell with an isolated double stranded nucleic acid (dsNA) in an amount effective to reduce expression of a target gene in a cell. The dsNAs of the invention possess a single stranded extension (in most embodiments, the single stranded extension comprises at least one modified nucleotide and/or phosphate back bone modification). Such single stranded extended Dicer-substrate siRNAs (DsiRNAs) were demonstrated to be effective RNA inhibitory agents compared to corresponding double stranded DsiRNAs.

Abstract: A consist communication system is disclosed for use with a train consist. The system may have a sensor associated with a component of the consist and configured to generate a signal indicative of a performance parameter of the component. The system may further include a controller, a fluid conduit, at least a first cable disposed within the fluid conduit and configured to transmit the signal from the sensor to the controller, and a glad-hand coupling fixedly connected to an end of the fluid conduit.

Abstract: The present invention relates to lipid compounds and uses thereof. In particular, the compounds include a class of cationic lipids having an amine moiety, such as an amino-amine or an amino-amide moiety. The lipid compounds are useful for in vivo or in vitro delivery of one or more agents (e.g., a polyanionic payload or an antisense payload, such as an RNAi agent).

Abstract: A system includes a router transceiver unit that is configured to be disposed on-board a vehicle system. The vehicle system may have at least a source vehicle and a separate linked vehicle that are mechanically linked with each other to travel together along a route and that are communicatively linked with each other through a system network of the vehicle system. The router transceiver unit is configured to be communicatively coupled to a requesting operational component of the source vehicle and the system network. The router transceiver unit is also configured to receive a local data packet from the requesting operational component that is directed toward a target operational component of the linked vehicle. The router transceiver unit includes an encapsulation module that is configured to transform the local data packet into an in-tunnel data packet, where the local and in-tunnel data packets have different packet formats.

Abstract: The invention provides compositions and methods for reducing expression of a target gene in a cell, involving contacting a cell with an isolated double stranded nucleic acid (dsNA) in an amount effective to reduce expression of a target gene in a cell. The dsNAs of the invention possess a pattern of deoxyribonucleotides (in most embodiments, the pattern comprises at least one deoxyribonucleotide-deoxyribonucleotide base pair) designed to direct the site of Dicer enzyme cleavage within the dsNA molecule. Deoxyribonucleotides of the dsNA molecules of the invention are located within a region of the dsNA that can be excised via Dicer cleavage to generate an active siRNA agent that no longer contains the deoxyribonucleotide pattern (e.g., deoxyribonucleotide-deoxyribonucleotide base pairs). Such DNA-extended Dicer-substrate siRNAs (DsiRNAs) were demonstrated to be more effective RNA inhibitory agents than corresponding double stranded RNA-extended DsiRNAs.

Abstract: A method of assembling a combustion system for a gas turbine engine includes providing a combustion chamber frame, an inner casing structure, and an outer casing structure. The method also includes mounting the combustion chamber frame between the inner casing structure and the outer casing structure such that the combustion chamber frame is coupled to the inner casing structure and the outer casing structure.

Abstract: The present disclosure relates to a monitor for a blowout preventer of a wellbore. The blowout preventer includes a housing, rams, and an actuator comprising a cylinder with a piston. The piston is operatively connectable with the ram and movable therewith. The monitor includes a monitor base operatively connectable to the cylinder (the monitor base having an interior side inside the cylinder and an exterior side outside the cylinder), an interior plate positionable inside the cylinder about the interior side of the base (the interior plate operatively connectable to the piston and movable therewith), an exterior plate positionable outside the cylinder about the exterior surface of the monitor base (the exterior plate coupled by magnets to the interior plate and rotatable therewith), and a trigger sensor operatively connectable about the monitor base and the exterior plate to detect rotation thereof whereby a position of the ram may be determined.

Abstract: The invention provides compositions and methods for reducing expression of a target gene in a cell, involving contacting a cell with an isolated double stranded nucleic acid (dsNA) in an amount effective to reduce expression of a target gene in a cell. The dsNAs of the invention possess a pattern of deoxyribonucleotides (in most embodiments, the pattern comprises at least one deoxyribonucleotide-deoxyribonucleotide base pair) designed to direct the site of Dicer enzyme cleavage within the dsNA molecule. Deoxyribonucleotides of the dsNA molecules of the invention are located within a region of the dsNA that can be excised via Dicer cleavage to generate an active siRNA agent that no longer contains the deoxyribonucleotide pattern (e.g., deoxyribonucleotide-deoxyribonucleotide base pairs). Such DNA-extended Dicer-substrate siRNAs (DsiRNAs) were demonstrated to be more effective RNA inhibitory agents than corresponding double stranded RNA-extended DsiRNAs.

Abstract: The present invention is based, at least in part, upon discovery of a process for identifying phase changing peptides. Such phase changing peptides are capable of enhancing in vitro and in vivo delivery of oligonucleotides (e.g., dsRNAs) in lipidic, vesicular, micellar and/or naked oligonucleotide formulations.

Abstract: A coupling for a communication conduit is disclosed for use with a train consist. The coupling may include a nipple configured for insertion within a fluid conduit, a flange configured to engage an end of the fluid conduit, a retention member configured to engage an outer surface of the fluid conduit, a first fitting extending from the flange away from the nipple, and, a retaining tab configured to retain a second fitting of another coupling in engagement with the first fitting. The coupling may further include a closure mechanism configured to close off an end of the first fitting and movable during engagement with the second fitting to open the end of the first fitting.

Abstract: An electrical connector comprising a housing having at least one terminal receiving passageway, an opening positioned proximate at least one terminal receiving passageway, a primary latch for latching the electric connector to a mating connector, and a slot extending longitudinally along side the primary latch. The connector has a connector position assurance member (CPA) positionable in the opening and having a locking latch which is laterally offset from the primary latch of the housing. The CPA has first and second longitudinal positions. The first position where the CPA is in a partially inserted position and a second position where the housing is in a fully mated position within a mating connector housing and the CPA is in a fully inserted position.