Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems.

Abstract: The present disclosure generally relates to systems, methods and compositions related to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and components thereof. The present disclosure also relates to methods, systems, and compostions modified to reduce immunogenicity. Additionally, the present disclosure relates to methods for developing or designing CRISPR-Cas system based therapy or therapeutics.

Abstract: Systems and methods described herein relate to the efficient handling of data purge requests in the context of a distributed storage system. A plurality of data purge requests is stored in a first data structure. The data purge requests may be grouped into batches that are processed at least partially in parallel. A first data purge request from the plurality of data purge requests is successfully processed, and is moved from the first data structure to a second data structure. Processing of a second data purge request from the plurality of data purge requests is unsuccessful. The second data purge request is retained in the first data structure. Purge status data is generated based on the first data purge request being in the second data structure and the second data purge request being in the first data structure. The purge status data may be presented at a user device.

Abstract: Integrated circuit structures having patch spacers, and methods of fabricating integrated circuit structures having patch spacers, are described. For example, an integrated circuit structure includes a stack of horizontal nanowires. A gate structure is vertically around the stack of horizontal nanowires, the stack of horizontal nanowires extending laterally beyond the gate structure. An internal gate spacer is between vertically adjacent ones of the stack of horizontal nanowires and laterally adjacent to the gate structure. An external gate spacer is along sides of the gate structure and over the stack of horizontal nanowires, the external gate spacer having one or more patch spacers therein.

Abstract: Techniques to form semiconductor devices can include one or more via structures having substrate taps. A semiconductor device includes a gate structure around or otherwise on a semiconductor region (or channel region). The gate structure may extend over the semiconductor regions of any number of devices along a given direction. The gate structure may be interrupted, for example, between two transistors with a via structure that extends through an entire thickness of the gate structure and includes a conductive core. The via structure has a conductive foot portion beneath the gate structure and a conductive arm portion extending from the conductive foot portion along a height of the gate structure. The conductive foot portion has a greater width along the given direction than any part of the conductive arm portion. The via structure may further include one or more dielectric layers between the conductive arm portion and the gate structure.

Abstract: Some embodiments of the present disclosure provide electric heating devices and methods for kilns of substrate glass, relating to the field of arrangements of electric heating structures for the kilns of substrate glass. To address the problem of insufficient melting in front zones of kilns of high-generation and large-tonnage substrate glass, a heating structure combining side-stack tin oxide electrode bricks and bottom-inserted molybdenum electrodes is designed. A comprehensive thermal efficiency of the kiln is determined by introducing an appropriate amount of gas and determining an energy consumption of glass melting and a thermal energy contribution of gas and electricity under an extraction volume. This leads to a novel electric heating device for the kiln of high-generation and large-feeding substrate glass and a method thereof, effectively solving the problem of insufficient melting and unstable convection in the front zone of the kiln.

Abstract: Devices and methods for measuring erosion resistance of electrofused high zirconia bricks are provided. The devices include a crucible, a crucible cover, a positioning axis, a plurality of holes, and a plurality of fixing members. The crucible cover is installed on an opening of the crucible, the positioning axis is installed at a center of the crucible cover, the plurality of holes are uniformly disposed along a circumferential direction of the crucible cover, and the plurality of fixing members are installed on the plurality of holes.

Abstract: Described herein are systems, methods, and compositions capable of targeting nucleic acids. Describe in certain exemplary embodiments herein are a class of small Cas proteins (Type II-D Cas proteins) and systems thereof. Also described in certain exemplary embodiments herein are methods of modifying target sequences using the class of small Cas proteins (Type II-D Cas proteins) and systems thereof described herein.

Abstract: The invention provides for delivery, engineering and optimization of systems, methods and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.

Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.

Abstract: Techniques are provided to form semiconductor devices that include through-gate structures (e.g., gate cut structures or conductive via structures) that have an airgap spacer between the structure and the adjacent gate electrode. In an example, a semiconductor device includes a gate structure around or otherwise on a semiconductor region (or channel region) that extends from a first source or drain region to a second source or drain region. A through-gate structure may extend in a third direction through an entire thickness of the gate structure and adjacent to the semiconductor region along the second direction. The through-gate structure may be a dielectric structure (e.g., a gate cut) or a conductive structure (e.g., a via). In either case, an airgap spacer exists between the through-gate structure and the gate structure.

Abstract: A hybrid powertrain and a vehicle are provided. The hybrid powertrain includes a first electrical machine, an engine, a main shaft, a clutch assembly, a second electrical machine, and a power battery. One end of the main shaft is connected to an output shaft of the engine, and another end of the main shaft is selectively connected to an electrical machine shaft of the first electrical machine through the clutch assembly. The first electrical machine is configured to selectively output power to a first wheel end through the clutch assembly. The second electrical machine includes a rotor and a stator, the rotor is configured to rotate relative to the stator, and the rotor is fixedly connected to the main shaft. The first electrical machine and the second electrical machine are connected to the power battery.

Abstract: Techniques are provided to form semiconductor devices where portions of the gate structure (e.g., foot structures) adjacent to the subfins have been removed. A semiconductor device includes a gate structure around or otherwise on a semiconductor region. The gate structure includes a gate dielectric and a gate electrode. The gate structure may be interrupted, for example, between two transistors with a gate cut that extends through an entire thickness of the gate structure and includes dielectric material to electrically isolate the portions of the gate structure on either side of the gate cut. The gate cut includes dielectric lobe structures that extend outwards from the sidewalls of the gate cut. The lobe structures effectively replace foot structures of the gate structure between the gate cut and subfin portions of the semiconductor fins. Removing the gate foot structures contributes to the reduction of the parasitic capacitance in the semiconductor device.

Abstract: A phase shifter includes first and second dielectric substrates opposite to each other and a plurality of phase shift units between the first and the second dielectric substrates; the phase shift unit includes first and second electrode layers and an adjustable dielectric layer between the first and the second electrode layers; orthographic projections of the first and second electrode layers on the first dielectric substrate at least partially overlap each other, and at least one accommodation cell of the phase shift unit is defined in a region where the orthographic projections of the first and second electrode layers on the first dielectric substrate overlap each other; the adjustable dielectric layer is in at least the accommodation cell; and cell gaps of the accommodation cells of at least a part of the plurality of phase shift units are different from each other.

Abstract: A frame for a pool that may be installed as a series of frame assemblies is disclosed. The frame assemblies may comprise a frame member, a sleeve comprising an end wall and a protrusion extending from the end wall, and a joint configured to receive the frame within the sleeve that fits within an opening of the joint, and receive a portion of the protrusion of the sleeve to lock the protrusion in place. The sleeve may comprise one or more ribs on an outer wall of the sleeve.

Abstract: The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect broth DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

Abstract: Provided herein are compositions, systems, and methods for delivering cargo to a target cell. The compositions, systems, and methods comprise one or more polynucleotides encoding one or more LTR retroelement polypeptides for forming a delivery vesicle and one or more capture moieties for packaging a cargo within the delivery vesicle. The one or more LTR retroelement polypeptides for forming a delivery vesicle may comprise two or more of an LTR retroelement gag protein, a retroelement envelope protein, an LTR retroelement reverse transcriptase, or a combination thereof. The LTR retroelement polypeptide alone, the LTR retroelement envelope protein alone, or both the LTR retroelement-derived polypeptide and LTR retroelement envelope protein may be endogenous.

Abstract: Described herein are engineered paraneoplastic Ma protein (PNMA) capable of forming a capsid. In some embodiments, the engineered PNMA proteins comprise one or more modifications that enhance binding or loading of a cargo into the capsid, one or more modifications that modify cell-specificity of the capsid, one or more modifications that enhance intracellular delivery of the capsid, or a combination thereof. Also described herein are delivery systems comprising capsids comprising an engineered PNMA protein and a cargo.

Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA or RNA-targeting systems comprising a novel DNA or RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.

Abstract: A computer implemented method includes receiving a start date and an end date specifying a future absence or away-from-work time period for a user, generating an away from office communication specifying the away-from-work time period, retrieving via the computer a list of collaborators based on frequency and duration of recent interactions with the user, receiving via the computer a selection of collaborators from the list, identifying meetings involving the user during the away-from-work time period, generating via the computer a list of user viewable items for the user to complete prior to the away-from-work time period, and generating via the computer a list of items for the user to attend to upon return from the away-from-work time period for presentation to the user upon return.