Abstract: The present invention provides methods and systems for real-time measurements of PCR with multiplexing capability. Certain embodiments relate to methods and systems that use fluorescently encoded superparamagnetic microspheres for the immobilization of amplification products during the PCR process, and an imaging chamber of a measurement device that is also capable of controllable thermal cycling for assisting the PCR process.

Abstract: An extraction method for preparing samples for analytical analysis is disclosed. The method includes the steps of placing a sample matrix containing one or more analytes in a heat conductive sample cup, positioning the heat conductive sample cup in a pressure-resistant reaction chamber, dispensing solvent into the heat conductive sample cup, dispersing the solvent and the sample matrix in the sample cup in the reaction chamber, heating the sample matrix and the extraction solvent in the heat conductive sample cup in the reaction chamber to a temperature at which the dispensed solvent generates an above-atmospheric pressure, and releasing the extraction solvent extract from the sample cup at atmospheric pressure.

Abstract: Lenses are designed using the corneal topography or wavefront measurements of the eye derived by subtracting the optical power of the eye after orthokeratology treatment from the optical power before orthokeratology treatment.

Abstract: Contact lenses incorporate high plus or add power profiles that at least one of slow, retard or preventing myopia progression and minimize halo effect. The lens includes a center zone with a negative power for myopic vision correction; and at least one treatment zone surrounding the center zone, the at least one treatment zone having a power profile that increases from an outer margin of the center zone to a positive power within the at least one treatment zone of greater than +5.00D.

Abstract: The present disclosure relates to ophthalmic devices such as ophthalmic lenses. An ophthalmic device may comprise an ophthalmic lens for at least one of slowing, retarding or preventing myopia progression. The ophthalmic lens may comprise a center zone with a negative power for myopic vision correction; and at least one treatment zone surrounding the center zone, the at least one treatment zone having a power profile comprising an ADD power, the at least one treatment zone having a surface shape comprising a portion of a generally toroidal shape, wherein the at least one treatment zone is arranged as to form a continuous surface with the center zone.

Abstract: Ophthalmic lenses incorporate multifocal properties for the purpose of slowing, retarding, controlling or preventing myopia development or progression, correcting presbyopic vision or allowing extended depth of focus. The lens has electronically controlled adjustable focus where the change in focus oscillates so rapidly that it is imperceptible to human vision.

Abstract: Provided herein are pharmaceutical compositions and kits useful for sensitizing a microorganism or a population of microorganisms to a quinolone antibiotic. In a particular aspect, a carbon source and an electron acceptor can sensitize an antibiotic persistent microorganism to treatment with a fluoroquinolone antibiotic. Methods for sensitizing a microorganism to a quinolone antibiotic, reducing the density-dependent persistence (DDP) of an antibiotic resistant microorganism, and reducing the number of persistent cells in a population are also provided. Theses compositions and methods are useful in treating infections resulting from high-density bacterial cultures, such as pneumonia, genitourinary infections, biofilms, prosthetic graft infections, sepsis, and endovascular infections.

Abstract: The present disclosure is related to an engineered nucleic acid encoding a post-poly A signal RNA 3? to a terminator for expression of protein, and/or non-coding RNA. Also provided herein are methods for reducing epigenetic silencing, genetic modification, transcriptional regulation of the engineered nucleic acid described herein.

Abstract: Contact lenses incorporate high plus or add power profiles that at least one of slow, retard or preventing myopia progression and minimize halo effect. The lens includes a center zone with a negative power for myopic vision correction; and at least one treatment zone surrounding the center zone, the at least one treatment zone having a power profile that increases from an outer margin of the center zone to a positive power within the at least one treatment zone of greater than +5.00 D.

Abstract: The invention controls a power cinching function of a motor vehicle door latch in order to improve door seal performance under conditions wherein vibrations could otherwise reduce seal effectiveness. With the door latched closed in a flush condition, the vehicle speed is compared to a slow-speed threshold. When the vehicle speed is greater than the slow-speed threshold, then a vehicle vibration parameter is compared to a vibration threshold. When the vibration parameter is greater than the vibration threshold, then the power cinching function is activated to move the door to to a subflush condition which increases a compression of the seal between the door and a vehicle door frame. When the door is in the subflush condition, the vehicle speed continues to be compared to the slow-speed threshold, and when the vehicle speed is less than the slow-speed threshold then the door is released back to the flush condition.

Abstract: Software build solutions are tailored to cache a build graph. The build graph is a dependency graph used to guide processes performed during a software build. Build graph caching provides faster builds after changes are made to source code files, allowing the retrieval from cache previously constructed build graph content unaffected by the change. The build graph cache includes a key-value store with an API providing StoreBuildGraph and FetchBuildGraph routines. Keys include fingerprints (hashes) of graph input parameter values, such as specification file paths, enumerated directories, and environment variables. Fingerprints may be combined by concatenation and further hashing. The API may be build-language agnostic. Pseudocode for cache store and fetch routines, and examples of their operation, are disclosed.

Abstract: Contact lenses incorporate high plus or add power profiles that at least one of slow, retard or preventing myopia progression and minimize halo effect. The lens includes a center zone with a negative power for myopic vision correction; and at least one treatment zone surrounding the center zone, the at least one treatment zone having a power profile that increases from an outer margin of the center zone to a positive power within the at least one treatment zone of greater than +5.00 D.

Abstract: Disclosed herein are hydrogels comprising a polynucleotide-based structural component. Methods of altering a property of a hydrogel based on user-defined nucleic acid input sequences are also disclosed. In addition, various applications are described that utilize these hydrogels and methods.

Abstract: The invention controls a power cinching function of a motor vehicle door latch in order to improve door seal performance under conditions wherein vibrations could otherwise reduce seal effectiveness. With the door latched closed in a flush condition, the vehicle speed is compared to a slow-speed threshold. When the vehicle speed is greater than the slow-speed threshold, then a vehicle vibration parameter is compared to a vibration threshold. When the vibration parameter is greater than the vibration threshold, then the power cinching function is activated to move the door to to a subflush condition which increases a compression of the seal between the door and a vehicle door frame. When the door is in the subflush condition, the vehicle speed continues to be compared to the slow-speed threshold, and when the vehicle speed is less than the slow-speed threshold then the door is released back to the flush condition.

Abstract: An instrument system for acid digestion is disclosed. The instrument includes a heating block, a reaction vessel formed of a polymer that is resistant to acid and other chemical attack at temperatures above 150° C. and that has a structure (thickness, etc.) sufficient to withstand pressures above atmospheric, a metal sleeve surrounding the polymeric reaction vessel, and an opening in the block that has a cross-section corresponding to the cross-section of the metal sleeve.

Abstract: Software build solutions are tailored to cache a build graph. The build graph is a dependency graph used to guide processes performed during a software build. Build graph caching provides faster builds after changes are made to source code files, allowing the retrieval from cache previously constructed build graph content unaffected by the change. The build graph cache includes a key-value store with an API providing StoreBuildGraph and FetchBuildGraph routines. Keys include fingerprints (hashes) of graph input parameter values, such as specification file paths, enumerated directories, and environment variables. Fingerprints may be combined by concatenation and further hashing. The API may be build-language agnostic. Pseudocode for cache store and fetch routines, and examples of their operation, are disclosed.

Abstract: Systems and methods for image recognition are provided. A style-transfer neural network is trained for each real image to obtain a trained style-transfer neural network. The texture or style features of the real images are transferred, via the trained style-transfer neural network, to a target image to generate styled images which are used for training an image-recognition machine learning N model (e.g., a neural network). In some cases, the real images are clustered and representative style images are selected from the clusters.

Abstract: Disclosed herein are a high-throughput continuous culture system and novel methodologies for the experimental evolution of natural and synthetic microbes using the continuous culture system. The microbial culture is exposed to a stress ramp function which is overlaid on top of a culture fitness function. The amount of stress applied to the culture is increased in response to increased fitness of the microbial culture.

Abstract: An extraction method for preparing samples for analytical analysis is disclosed. The method includes the steps of placing a sample matrix containing one or more analytes in a heat conductive sample cup, positioning the heat conductive sample cup in a pressure-resistant reaction chamber, dispensing solvent into the heat conductive sample cup, dispersing the solvent and the sample matrix in the sample cup in the reaction chamber, heating the sample matrix and the extraction solvent in the heat conductive sample cup in the reaction chamber to a temperature at which the dispensed solvent generates an above-atmospheric pressure, and releasing the extraction solvent extract from the sample cup at atmospheric pressure.