Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Abstract: Methods, compositions and kits are provided herein for insertional modification of nucleic acids by, for example transposase-mediated covalent insertion of insertion sequence into a sample nucleic acid molecule. Using sequence of the insertion to direct amplification of adjacent nucleic acid sequence, and using bar codes to map amplified sequence to partitions, one can map sample nucleic acid sequence to single molecules of the nucleic acid sample that are derived directly from the sample nucleic acid molecule.

Abstract: Methods, compositions and kits are provided herein for insertional modification of nucleic acids by, for example transposase-mediated covalent insertion of insertion sequence into a sample nucleic acid molecule. Using sequence of the insertion to direct amplification of adjacent nucleic acid sequence, and using bar codes to map amplified sequence to partitions, one can map sample nucleic acid sequence to single molecules of the nucleic acid sample that are derived directly from the sample nucleic acid molecule.

Abstract: A footwell assembly for a vehicle is disposed herein. The footwell assembly includes at least one foot pedal disposed in the footwell and operable between a use position and a stowed position. A receiving member is positioned within the footwell and is defining a recess, wherein the at least one foot pedal is configured to nest within the recess while in the stowed position. A cover feature is operably coupled to the receiving member and is configured to cover the at least one foot pedal when the at least one foot pedal is nested within the recess of the receiving member in the stowed position.

Abstract: Per- and polyfluoroalkyl substances (PFAS) are destroyed by oxidation in supercritical conditions. PFAS in water can be concentrated and prepared for destruction in a pretreatment phase. Following annihilation of the PFAS in supercritical conditions to levels below 5 parts per trillion (ppt), the water effluent can be used to recover heat, returned to sub-critical conditions, and then released back into the environment.

Abstract: A footwell assembly for a vehicle is disposed herein. The footwell assembly includes at least one foot pedal disposed in the footwell and operable between a use positon and a stowed position. A receiving member is positioned within the footwell and is defining a recess, wherein the at least one foot pedal is configured to nest within the recess while in the stowed position. A cover feature is operably coupled to the receiving member and is configured to cover the at least one foot pedal when the at least one foot pedal is nested within the recess of the receiving member in the stowed position.

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Abstract: A multi-stack fuel cell system includes upper and lower housings defining interior chambers in corresponding upper and lower stacks of fuel cells are disposed. A heat exchanger assembly is fluidly coupled with the interior chambers. The heat exchanger assembly receives input fuel and/or input air from outside of the housings and receives outgoing fuel and/or outgoing air from the fuel cells. The heat exchanger assembly heats the input fuel and/or the input air, and/or cools the outgoing fuel and/or the outgoing air. The heat exchanger assembly may be disposed between the upper and lower housings. The upper housing an upper stack of fuel cells and/or the heat exchanger assembly may assist in compressing the fuel cells in the lower stack against each other.

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Abstract: Disclosed herein are compositions and methods related to the elimination of molecules of a selected sequence from a nucleic acid sample or from an sequence dataset resulting from the sequencing of a sample, for example to exclude such molecules from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.

Abstract: Apparatus and methods for providing small cell backhaul are disclosed. A network node that acts as a gateway for a local communication network to a main communication network through a bonded link with the main communication network also provides a wireless communication module with a backhaul communication link to the main communication network through its bonded link. A switch module in the network node switches communication traffic between the local communication network, the wireless communication module and the bonded link to the main communication network. The network node may power the wireless communication module utilizing remote power provided by the main communication network, the local communication network, and/or a local source of power. Apparatus and methods for providing a transparent bonded link through a network access multiplexer are also disclosed, including management of the bonded link and of nodes subtending from the bonded link.

Abstract: Disclosed herein are compositions and methods related to the elimination of molecules of a selected sequence from a nucleic acid sample or from an sequence dataset resulting from the sequencing of a sample, for example to exclude such molecules from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Abstract: A method of managing water entering through a hood vent includes steps of: collecting the water in a water collection tray of the hood vent, delivering the water, through a first water drain of the hood vent, to a water channel of a hood insulator and discharging the water from a second water drain in the hood insulator.

Abstract: Apparatus and methods for providing small cell backhaul are disclosed. A network node that acts as a gateway for a local communication network to a main communication network through a bonded link with the main communication network also provides a wireless communication module with a backhaul communication link to the main communication network through its bonded link. A switch module in the network node switches communication traffic between the local communication network, the wireless communication module and the bonded link to the main communication network. The network node may power the wireless communication module utilizing remote power provided by the main communication network, the local communication network, and/or a local source of power. Apparatus and methods for providing a transparent bonded link through a network access multiplexer are also disclosed, including management of the bonded link and of nodes subtending from the bonded link.

Abstract: Rapid nucleic acid libraries, methods of generation, kits, and compositions relating to library synthesis, including reagents, intermediaries and final products are disclosed herein. The disclosure enables rapid synthesis of libraries that allow independent verification of sequence information and rapid identification of sequence information with template of origin.

Abstract: Rapid nucleic acid libraries, methods of generation, kits, and compositions relating to library synthesis, including reagents, intermediaries and final products are disclosed herein. The disclosure enables rapid synthesis of libraries that allow independent verification of sequence information and rapid identification of sequence information with template of origin.

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Abstract: A method of managing water entering through a hood vent includes steps of: collecting the water in a water collection tray of the hood vent, delivering the water, through a first water drain of the hood vent, to a water channel of a hood insulator and discharging the water from a second water drain in the hood insulator.

Abstract: An engine air induction system includes an air box and a hood insulator. The air box has an open top and a perimeter seal extending continuously around the open top. The hood insulator has a body including an air box closeout feature providing a continuous interface with the perimeter seal when a hood assembly incorporating the hood insulator is in a closed position. A related method of sealing an open top on an air box of a motor vehicle is also disclosed.