Abstract: Disclosed herein are methods of long range target specific amplification and sequencing using an RNA intermediate synthesized directly from the target including using hairpin adaptors having a double stranded promoter and an overhang which hybridizes with a reverse-complementary overhang on a target nucleic acid. RNA transcription eliminates clonal amplification of early synthesis errors. Approaches allow for the identification of target-adjacent sequence, such as sequence adjacent to a repeat element target. Also disclosed herein are compositions and kits for amplification and sequencing.

Abstract: A vehicle includes a metallic floor pan. A plurality of trim components defining a foot well is proximate the floor pan. A flooring component is coupled with the floor pan which includes a polymeric structural layer. An insulation layer is positioned on the structural layer. A sealing layer is coupled with the floor pan and extends to seal the insulation layer against the structural layer.

Abstract: A hood assembly includes a hood body and a hood vent water management system. The hood vent water management system includes a hood vent having a mounting bracket including a water collection tray and a first water drain and a hood insulator including a water channel and a second water drain. The hood vent water management system diverts water entering through the hood vent away from an underlying engine compartment component. A related method is also disclosed.

Abstract: An antenna array (106) which in use emits radiation in two respectively orthogonally polarised directions, the array including a plurality of elements, the elements including at least one element of a first type and at least four elements of a second type wherein the element of the first type comprises part of two balanced feeds with two elements of the second type and the element of the first type is capacitively coupled to two further elements of the second type, wherein the elements used to produce radiation in a first direction lie in a first plane (104), the elements used to produce radiation in a second direction lie in a second plane (102), and the first and second planes are spaced apart, and the element of the first type includes two portions, one of which lies in the first plane and one of which lies in the second plane.

Abstract: The disclosure provides a method of inking a polymer pen lithography tip array (“PPL tip array”), including applying an ink composition to a PPL tip array using at least two spray applicators to deposit the ink as a non-uniform ink layer. The disclosure further provides a method of depositing at least two ink compositions on PPL tip array, including applying a first ink to a first defined area using a first spray applicator, and applying a second ink to a second defined area using a second spray applicator. The disclosure further provides a method of scaling a linear ink composition gradient provided by at least two spray applicators to a PPL tip array having a length, L, including positioning the spray applicators at a distance of about 5.0 L to about 8.0 L relative to the surface of the PPL tip array, positioning the spray applicators at a distance of about 0.5 L to about 3.0 L relative to each other, and applying an ink composition.

Abstract: A vehicle includes a metallic floor pan. A plurality of trim components defining a foot well is proximate the floor pan. A flooring component is coupled with the floor pan which includes a polymeric structural layer. An insulation layer is positioned on the structural layer. A sealing layer is coupled with the floor pan and extends to seal the insulation layer against the structural layer.

Abstract: A control system for a water treatment apparatus that includes a control valve assembly having a servo chamber in which a stationary port defining signal ports is located. A regeneration control disc sealingly engages and rotates on a planar surface of the port disc and selectively communicates fluid signals to water pressure operated components within the control valve. An electric motor located in a dry chamber is operatively coupled to the regeneration control disc and rotates the disc during a regeneration cycle. An encoder coupled to the control disc monitors its position and movement. A turbine assembly monitors water treated or to be treated and is electronically coupled to a regeneration controller. A sensor emits pulses related to rotation of the usage turbine and communicates these pulses to the controller which uses this information to determine when a regeneration is needed whereupon the drive motor is appropriately energized.

Abstract: A hood assembly includes a hood body and a hood vent water management system. The hood vent water management system includes a hood vent having a mounting bracket including a water collection tray and a first water drain and a hood insulator including a water channel and a second water drain. The hood vent water management system diverts water entering through the hood vent away from an underlying engine compartment component. A related method is also disclosed.

Abstract: Methods, compositions and kits are provided herein for insertional modification of nucleic acids by, for example transposase-mediated covalent insertion of insertion sequence into a sample nucleic acid molecule. Using sequence of the insertion to direct amplification of adjacent nucleic acid sequence, and using bar codes to map amplified sequence to partitions, one can map sample nucleic acid sequence to single molecules of the nucleic acid sample that are derived directly from the sample nucleic acid molecule.

Abstract: The invention provides an antenna array including an array of unit cells, each unit cell including two annular elements of a first type and two annular elements of a second type wherein in each unit cell: the elements of the first type comprise a balanced feed to produce radiation in a first polarisation direction, the elements of the second type comprise a balanced feed to produce radiation in a second polarisation direction, and each element of the first type is capacitively coupled to a further element of the first type located in an adjacent unit cell, and each element of the second type is capacitively coupled to a further element of the second type located in an adjacent unit cell.

Abstract: An engine air induction system includes an air box and a hood insulator. The air box has an open top and a perimeter seal extending continuously around the open top. The hood insulator has a body including an air box closeout feature providing a continuous interface with the perimeter seal when a hood assembly incorporating the hood insulator is in a closed position. A related method of sealing an open top on an air box of a motor vehicle is also disclosed.

Abstract: An adjustable food holding apparatus, said apparatus comprising: at least one fork assembly selectively, releasably, and slidably disposed along a rotisserie rod.

Abstract: A compressor may include a scroll member, a hub, a discharge valve and a bypass valve. An end plate of the scroll member includes a recess, a discharge passage in communication with the recess, and a bypass passage in communication with the recess and disposed radially outward relative to the discharge passage. The hub may be received in the recess and may include a central opening in communication with the discharge passage and the bypass passage. The discharge valve may be disposed between the hub and the end plate and may control fluid flow through the discharge passage. The bypass valve may be disposed between the hub and the end plate and may be movable between a first position restricting fluid flow through the bypass passage and a second position allowing fluid to flow through the bypass passage, around the discharge valve and through the central opening.

Abstract: Technology is provided for an aqueous solution constituent analyzer. The analyzer includes an ultraviolet light emitting diode (LED) with a current source providing variable current thereto. A spectrometer is positioned for receiving light from the LED transmitted through an aqueous solution. A controller receives radiant flux data for a plurality of wavelengths and determines, based on the radiant flux data, a usable number of the plurality of wavelengths that satisfies a relative uncertainty threshold. The controller can increase the current to the LED if the usable number of wavelengths is less than a minimum threshold and calculate a concentration of a constituent of interest in the solution. The controller can also determine a peak wavelength of the plurality of wavelengths having the greatest intensity value, and decrease the current level to the LED if the peak wavelength has an intensity value greater than a saturation value for the spectrometer.

Abstract: Provided here are hard transparent polymer pen arrays, methods of printing indicia using the same, and methods of preparing the same.

Abstract: The present invention relates to a device and method for providing immediate additional ballistic resistant portable personal protection for first responders, such as Police, Fire, EMS, and military personnel, during an emergency event. The device, configured for insertion into either the driver or passenger interior floor of an emergency vehicle is comprised of a molded shell with layers comprising a ballistic resistant layer that both provides vehicle floor matting and fits seamlessly into the contours of the emergency vehicle foot well and interior floor. For easy removal and immediate use as a directional portable personal protection device, handles are provided on the shell such that the handles do not interfere with driving.

Abstract: In accordance with an embodiment of the disclosure, a tip array can include an elastomeric tip substrate layer comprising a first surface and an oppositely disposed second surface, the tip substrate layer being formed from an elastomeric material; a plurality of tips fixed to the first surface, the tips each comprising a tip end disposed opposite the first surface, the tips having a radius of curvature of less than about 1 micron; and an array of heaters disposed on the second surface of the tip substrate layer and configured such that when the tip substrate layer is heated by a heater, a tip disposed in a location of a heated portion of tip substrate layer is lowered relative to a tip disposed in a location of an unheated portion of the tip substrate layer.

Abstract: A multi-stack fuel cell system includes upper and lower housings defining interior chambers in corresponding upper and lower stacks of fuel cells are disposed. A heat exchanger assembly is fluidly coupled with the interior chambers. The heat exchanger assembly receives input fuel and/or input air from outside of the housings and receives outgoing fuel and/or outgoing air from the fuel cells. The heat exchanger assembly heats the input fuel and/or the input air, and/or cools the outgoing fuel and/or the outgoing air. The heat exchanger assembly may be disposed between the upper and lower housings. The upper housing an upper stack of fuel cells and/or the heat exchanger assembly may assist in compressing the fuel cells in the lower stack against each other.

Abstract: The present invention relates to a device and method for providing immediate additional ballistic resistant portable personal protection for first responders, such as Police, Fire, EMS, and military personnel, during an emergency event. The device, configured for insertion into either the driver or passenger interior floor of an emergency vehicle is comprised of a molded shell with layers comprising a ballistic resistant layer that both provides vehicle floor matting and fits seamlessly into the contours of the emergency vehicle foot well and interior floor. For easy removal and immediate use as a directional portable personal protection device, handles are provided on the shell such that the handles do not interfere with driving.

Abstract: A capacitor with improved lead frame attachment is described wherein the improved lead frame attachment mitigates defects. The capacitor comprises parallel conductive internal electrodes of alternating polarity with a dielectric between the conductive internal electrodes. A first copper undercoat is in electrical contact with the conductive internal electrodes of a first polarity and a second copper undercoat is in electrical contact with conductive internal electrodes of a second polarity. A first lead is in electrical contact with the first copper undercoat with a first solder between the first lead and the first copper undercoat. A second lead is in electrical contact with the second copper undercoat with a second solder between the second lead and the second copper undercoat.