Abstract: Described herein are methods for the expression and purification of Cas13a and methods for detecting target RNA using Cas13a.

Abstract: Techniques for predicting a manufacturer and/or contents of a received package are described herein. Images of a package may be received from cameras. A visual vector for the package in a vector space of package attributes may be generated using the images. Physical attributes of the package may be received. A subset of candidate packages may be determined by comparing the physical attributes of a plurality of historically received packages to the physical attributes of the package. A ranking of the subset of candidate packages may be determined by identifying a distance in the vector space of the package attributes between a vector for the subset of candidate packages and the visual vector for the package. An identifier associated with a particular package of the ranked subset of candidate packages may be obtained. Data for the particular package may be retrieved from a database using the identifier.

Abstract: Methods, apparatus, systems and articles of manufacture (e.g., physical storage media) to credit commercial broadcasts are disclosed. Example commercial crediting apparatus disclosed herein compare first identification information and first timing information obtained from a first monitored data feed with second identification information and second timing information in a second monitored data feed to identify a first final distributor code represented in the second monitoring data feed, the first identification information and the first timing information corresponding to a first commercial represented in the first monitored data feed. Disclosed example apparatus also access data that maps a first original distributor code to the first final distributor code, identify the first commercial in lineup data based on the first original distributor code, and adjust, based on a duration of the first commercial, a duration obtained for a first media program listed in the lineup data.

Abstract: The present invention provides a catalytic cracking reactor comprising a conduit, configured to allow the passage of a flow of catalyst particles, and an injection zone comprising a ring of feed injectors extending inwardly from the wall of reactor and angled to inject feed into the flow of catalyst particles, characterised in that the reactor also comprises a contacting device protruding into the reactor from the inner wall of said reactor upstream of the injection zone.

Abstract: Various embodiments include techniques for performing self-synchronizing remote memory operations in a multiprocessor computing system. During a remote memory operation in the multiprocessor computing system, a source processing unit transmits multiple segments of data to a destination processing. For each segment of data, the source processing unit transmits a remote memory operation to the destination processing unit that includes associated metadata that identifies the memory location of a corresponding synchronization object. The remote memory operation along with the metadata is transmitted as a single unit to the destination processing unit. The destination processing unit splits the operation into the remote memory operation and the memory synchronization operation. As a result, the source processing unit avoids the need to perform a separate memory synchronization operation, thereby reducing inter-processor communications and increasing performance of remote memory operations.

Abstract: A roasting fork cleaning assembly for cleaning food debris from a roasting fork includes a handle that includes a plurality of slidable sections such that the handle has a telescopically adjustable length. A grip is attached to the handle and a holder is attached to the handle. A collar is insertable into the holder thereby facilitating the roasting fork to be inserted into the collar when the collar is inserted into the holder. A plurality of bristles is each attached to the collar. Each of the bristles is directed toward a center of the collar thereby facilitating each of the bristles to abrade the roasting fork when the roasting fork is inserted into the collar. In this way the bristles can scrub food debris from the roasting fork.

Abstract: This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRSPR/Cas endonuclease systems having mutant Cas9 proteins that display reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system.

Abstract: The present invention pertains to a tag-free CM1 polypeptide and methods for improving homology directed repair (HDR) in a recipient cell using the same. Preferred donor template delivery vehicles include Adeno virus-associated vectors for delivering donor templates, and a preferred tag-free CM1 polypeptide called CM1tf. Isolated nucleic acids encoding tag-free CM1 polypeptides are also provided.

Abstract: A roller shade assembly includes a roller tube including a first end opposite a second end, the roller tube defining an opening longitudinally extending between the first and second ends, and an idler assembly partially received by the opening at the first end, the idler assembly including an idler housing, a plunger received by the idler housing, and a biasing member configured to apply a biasing force onto the plunger, wherein the plunger is configured to slide relative to the idler housing, and the plunger is configured to selectively engage a bracket member.

Abstract: An adhesive composition comprising a core shell vinyl ester and a urethane acrylate, which is suitable to be applied to a rough or uneven surface of a substrate to render a smooth surface. The present adhesive composition is particularly suitable to coat large objects made through additive manufacturing to form molds or prototypes.

Abstract: A stem sealing device for a valve can include a device body defining an annular shape; a stem seal defining an annular shape and configured to seal a gap between a valve stem of the valve and a valve body of the valve and positioned within the device body, the stem sealing device installable in and removable from the valve as an assembly without prior or subsequent removal of the stem seal; and a fastener configured to secure the device body to the valve body.

Abstract: According to one aspect, a system and method of exchanging GRUUs (Globally Routed User Agent URI (Uniform Resource Identifier)) between a first telephony-enabled device and a second telephony enabled device using a circuit-switched message is provided. Once exchanged, the telephony enabled devices can exchange SIP (session initiated protocol) communications routed by the GRUUs. Any one of the telephony-enabled devices can add a media component to the SIP communications. According to another aspect, a system and method of generating GRUUs is provided. According to another aspect, a system and method of handing off communications to a packet switched network from a circuit switched network is provided.

Abstract: The transmission and reception group delay in a front end structure of a mobile device may be determined using closed loop calibration. The closed loop may be a near field radiated closed loop between pairs of antennas in an antenna array of the mobile device. The delay based on time of transmission and time of reception may be measured for a plurality of pairs of antennas, from which the transmit and receive group delay within a single path may be determined. The propagation delay of the signal between antennas may be included in the group delay calibration for increased accuracy. In another implementation, a conducted closed loop, e.g., in the transceiver or in a radio frequency switching network may be used to calibrate the group delay. Pre-characterization of the delay caused by components between the closed loop and antennas may be included in the group delay calibration for increased accuracy.

Abstract: Systems and methods for tracking unintentional high amplitude muscle movements of a user and stabilizing a handheld tool are described. The method may include detecting motion of a housing of the handheld tool when manipulated by a user while the user is performing a task with a user-assistive device attached to an attachment arm of the handheld tool, and storing the detected motion in a memory of the handheld tool as motion data. Furthermore, the method may include controlling a first motion generating mechanism and a second motion generating mechanism by generating a first motion signal and a second motion signal that respectively drive the first motion generating mechanism in a first degree of freedom and the second motion generating mechanism in a second degree of freedom to stabilize motion of the user-assistive device attached to the attachment arm of the handheld tool.

Abstract: In various examples, a transaction type of a transaction from a processing resource of a plurality of processing resources sharing a bus may be determined and used to track bandwidth usage for the processing resource with respect to a time slot. Transactions that indicate usage of downstream bandwidth may be distinguished from transactions that do not indicate usage of downstream bandwidth. Bandwidth usage for a time slot may be tracked using one or more counters. The system may block or permit transactions from reaching the bus based at least on the counter exceeding a threshold value. The total allocation of bandwidth to the processing resources sharing a bus may be limited to a value that is less than a maximum capability of the bus to allow for headroom. Bandwidth coming from different lines and/or lanes and belonging to the same processing resource may be shared.

Abstract: An imaging system and methods including a gantry defining a bore and an imaging axis extending through the bore, and at least one support member that supports the gantry such that the imaging axis has a generally vertical orientation, where the gantry is displaceable with respect to the at least one support member in a generally vertical direction. The imaging system may be configured to obtain a vertical imaging scan (e.g., a helical x-ray CT scan), of a patient in a weight-bearing position. The gantry may be rotatable between a first position, in which the gantry is supported such that the imaging axis has a generally vertical orientation, and a second position, such that the imaging axis has a generally horizontal orientation. The gantry may be displaceable in a horizontal direction and the system may perform a horizontal scan of a patient or object positioned within the bore.

Abstract: A thermosetting composition comprising a crosslinkable component such as a vinyl ester and/or an unsaturated polyester, and an initiator component, such as cumene hydroperoxide or BPO or blends of cumene hydroperoxide and methyl ethyl ketone peroxide. The composition is suitable to be used in additive manufacturing to form objects such as molds or prototypes.

Abstract: An apparatus and method for radiation measurement are used to determine clearance of a rotating gas turbine component. The apparatus includes a probe body on a stationary component outward of the rotating component and a pair of sensor assemblies coupled to the probe body. Each sensor assembly includes a plurality of prisms coupled to the probe body Each prism of the plurality of prisms has a distinct angular orientation to direct radiation from the focusing lens to the rotating component surface off-axis with respect to an axis of the respective radiation source of the pair of radiation sources and with less directional variation than radiation from the respective radiation source.

Abstract: An apparatus for detecting radiation deflected from a rotating component includes a probe body on a stationary component radially outward of the rotating component; and a pair of arrival time sensors coupled to the probe body. Each arrival time sensor includes a light pipe that receives radiation deflected from the rotating component and that reduces directional variation of the radiation. A detector is optically coupled to each respective light pipe. The detector is positioned to receive the radiation with reduced directional variation from the light pipe. A method of detecting radiation from the rotating component using the apparatus determines a clearance between the rotating component (e.g., a turbomachine blade) and a stationary component (e.g., a casing).

Abstract: A method for providing a user agent (UA) with service identification data. The method includes an application server (AS) transmitting Session Initiation Protocol (SIP) data. The SIP data comprises an identifier of the AS and a service identifier for at least one service supported by the AS. The method further includes the UA receiving the SIP data.