Abstract: An electrical connector includes an insulation body, at least one first terminal and at least one second terminal, and a middle grounding plate. The insulation body has a base and a tongue located in a front end of the base. The first terminal and the second terminal are arranged in the base in an upper row and a lower row, and exposed to an upper surface and a lower surface of the tongue. The middle grounding plate has a body portion fixed to the tongue, and located between the first terminal and the second terminal, the first terminal has a first front edge, the second terminal has a second front edge, and the first front edge, the second front edge and an edge in a front end of the body portion are located in a same vertical plane.

Abstract: An electrical connector includes an insulation body having a base and a tongue located in a front end of the base, terminals arranged in upper and lower rows, and a middle grounding plate disposed in the base and extending to the tongue. Terminals in the upper and lower rows are arranged on upper and lower surfaces of the tongue, respectively. The middle grounding plate is located between the upper and lower rows of terminals, and having at least one first hole. An edge of each of two lateral sides of the middle grounding plate is inwardly depressed to form a first groove. The first hole is located between the two first grooves. The insulation body has a first insulation post and a second insulation post, the first insulation post protrudes into the first hole, and the second insulation post protrudes into the first grooves on the lateral sides.

Abstract: An electrical connector used for mating a mating connector includes an insulation body having a base portion and a tongue extending from the base portion, terminals fixedly disposed in the base portion and grouped into an upper row and a lower row, and a middle shielding sheet embedded in the tongue and located between the upper and lower rows. The tongue has upper and lower surfaces arranged opposite to each other, and a front surface connected to the upper and lower surfaces. The upper and lower rows of terminals are exposed on the upper and lower, respectively. The middle shielding sheet has a front end surface extending out of the front surface, a slot formed on the front end surface, and a first projection portion extending into the slot, overlapping a central line of the middle shielding sheet along a longitudinal direction, and located behind the front surface.

Abstract: The present invention is directed to novel methods and kits to be employed for preparing adapter-ligated amplicons or a sequencing library of a target DNA, respectively.

Abstract: The present invention is in the fields of molecular biology. The present invention is directed to novel compositions, methods and kits useful for the generation of nucleic acids from an RNA template and further nucleic acid replication. Specifically, the invention is directed to the generation and amplification of nucleic acids by reverse transcriptase-polymerase chain reaction.

Abstract: The invention relates to a method for the quantification of one or more nucleic acids in a sample, for example: making a sample available which contains at least one nucleic acid to be quantified, adding an oligonucleotide probe, the oligonucleotide probe comprising a sequence which can specifically hybridize to the nucleic acid to be quantified or to a common sequence of the nucleic acids to be quantified, incubating the sample under conditions which allow the hybridization of the oligonucleotide probe to the nucleic acid(s) to be quantified, incubating the sample under conditions which allow the extension of hybridized probes, the nucleic acid(s) serving as a template in each case, removing the non-hybridized probes from the sample and quantifying the hybridized oligonucleotide probes to measure the quantity of the nucleic acid(s) to be quantified. The invention also relates to a kit for carrying out said method.

Abstract: The present invention relates to methods and uses where a combination of (i) an enzyme exhibiting 5?->3? exonuclease activity and specifically degrading 5?-phosphorylated strands of double-stranded nucleic acids is used, and (ii) one or more primer pair(s), each primer being 5? phosphorylated, in a method comprising at least a first and a second amplification reaction of a first and a second template, respectively, for preventing contamination of the second amplification reaction with amplification products of the first amplification reaction, as well as novel methods and kits using the combination. Also, the present invention relates to kits comprising the enzyme and either 5?-phosphorylated primers or a polynucleotide kinase.

Abstract: An includes an insulation body, multiple terminals, an inner metal shell, and an outer metal shell. The insulation body has a base and a tongue located in a front end of the base. The terminals are fixedly arranged in the base in upper and lower rows. Each terminal has a contact portion exposed to an upper surface or a lower surface of the tongue. The inner metal shell has a covering portion disposed on the upper surface of the tongue, and an extending portion extending from the covering portion to the base, and covering the base. The outer metal shell wraps the inner metal shell.

Abstract: The invention is directed to novel methods, kits and uses to be employed for the generation of ligation-ready DNA amplicons of a target DNA by using 5?-phosphorylated primers.

Abstract: A method for planarizing a semiconductor device includes steps herein. A substrate is provided, on which a stop layer is formed. A trench is formed in the substrate. A first semiconductor film is deposited conformally on the stop layer and the trench. A second semiconductor film is deposited to fill the trench and cover the first semiconductor film. A chemical-mechanical polishing process is performed until the stop layer is exposed. A removal rate of the chemical-mechanical polishing process on the first semiconductor film is higher than that on the second semiconductor film. The first dielectric layer on the substrate selectively is removed.

Abstract: The present invention relates to methods and uses for the detection or quantification of newly-synthesized double-stranded target nucleic acid molecules in a sample during quantitative real-time polymerase chain reaction (qPCR) amplification. According to the invention, an intercalating dye recognizing double-stranded DNA molecules with higher affinity than single-stranded DNA molecules and a fluorophore-labeled oligonucleotide-probe being sequence specific for a target nucleic acid molecule are simultaneously employed, thus enabling quantification a specific target and total amount of a mixed nucleic acid population, and enabling assessing the cause of suboptimal PCR performance.

Abstract: The present invention relates to a method for real-time monitoring and/or quantification of newly-synthesized complementary deoxyribonucleic acid (cDNA) during a reverse transcription reaction of an ribonucleic acid (RNA) template in a sample, the method using a fluorogenic dye binding to RNA:cDNA hybrids. The present invention also relates to the use of this method as well as to kits employing the fluorogenic dye.

Abstract: A method for planarizing a semiconductor device includes steps herein. A substrate is provided, on which a stop layer is formed. A trench is formed in the substrate. A first semiconductor film is deposited conformally on the stop layer and the trench. A second semiconductor film is deposited to fill the trench and cover the first semiconductor film. A chemical-mechanical polishing process is performed until the stop layer is exposed. A removal rate of the chemical-mechanical polishing process on the first semiconductor film is higher than that on the second semiconductor film. The first dielectric layer on the substrate selectively is removed.

Abstract: Methods are described which utilize additives that ease secondary structure of DNA to improve sequencing performance. In one embodiment, said additive is betaine. The addition of betaine leads to decreased error rates.

Abstract: Methods are described which utilize modified sequencing primers that bind to template with high specificity and stability to improve sequencing performance. In one embodiment, the method utilizes sequencing primers having 3? and 5? ends, comprising a minor groove binder (MGB) molecule linked to the 5? end. In one embodiment said primer further comprises a 5? flap and said MGB molecule is linked to the 5? flap.

Abstract: Methods are described for the separation of microspheres covered with nucleic acids of interest from undesired microspheres and/or molecules. These separations may be negatively affected by the presence of non-specific interactions between nucleic acids or microspheres.

Abstract: An electrical connector, for mating a mating connector having two metal elastic sheets, includes: an insulation body having a base portion and a tongue located at a front end thereof; an upper and lower rows of terminals fixed to the base portion, each terminal having a contact portion exposed from an upper or lower surface of the tongue; a middle shielding sheet, fixed to the base portion and tongue, and located between the two terminal rows; two snap-fit portions disposed at two sides of the middle shielding sheet and exposed from two sides of the tongue, where the two metal elastic sheets buckle the snap-fit portions to prevent disengagement; and an outer metal casing wrapping peripheries of the base portion and tongue. When the mating connector is inserted into the electrical connector after it's assembling, the two snap-fit portions are fixed to the mating connector to ensure stable high-frequency performance.

Abstract: The present invention is in the fields of molecular biology. The present invention is directed to novel compositions, methods and kits useful for the generation of nucleic acids from an RNA template and further nucleic acid replication. Specifically, the invention is directed to the generation and amplification of nucleic acids by reverse transcriptase-polymerase chain reaction.

Abstract: The present invention relates to a method for detecting and/or for quantifying poly(A) RNA and/or mRNA, wherein a poly(dT) oligonucleotide which features a fluorescent dye and also a quencher is hybridized to the nucleic acid to be detected. Non-hybridized poly(dT) oligonucleotide is degraded by an added nuclease, and as a result, fluorescently labelled nucleotides are released and the resulting fluorescent signal is measured.

Abstract: The present invention relates to a method for detecting and/or for quantifying poly(A) RNA and/or mRNA, wherein a poly(dT) oligonucleotide which features a fluorescent dye and also a quencher is hybridized to the nucleic acid to be detected. Non-hybridized poly(dT) oligonucleotide is degraded by an added nuclease, and as a result, fluorescently labelled nucleotides are released and the resulting fluorescent signal is measured.