Abstract: The invention relates to a method for preparing a strand-specific library from an nucleic acid or preferably RNA sample, for RNA comprising the steps of: (i) optionally fragmenting said RNA sample, (ii) generating a plurality of first cDNA strands by subjecting said fragmented RNA to reverse transcription by using a reverse transcriptase and first oligonucleotide primers, (iii) generating a plurality of second cDNA strands by using a DNA polymerase, second oligonucleotide primers, and the plurality of first cDNA strands, and (iv) ligating adapters to the 3? and 5? termini of the of double-stranded cDNA, (v) wherein the first cDNA strand allows no adapter ligation at its 5? terminus and said second cDNA strand allows adapter ligation at its 5? terminus, or vice versa, and, (v) optionally cloning, sequencing or otherwise using the strand-specific library.

Abstract: A production method of a negative electrode active material for non-aqueous electrolyte secondary batteries containing particles of lithium-containing silicon compound includes: preparing particles of silicon compound containing a silicon compound (SiOx: 0.5?x?1.6); obtaining particles of lithium-containing silicon compound by making the particle of silicon compound contact with a solution A that contains lithium and has an ether-based solvent as a solvent; and heating the particles of the lithium-containing silicon compound. A production method of a negative electrode active material for non-aqueous electrolyte secondary batteries is capable of increasing battery capacity of the negative electrode active material and capable of improving the first time efficiency and cycle characteristics.

Abstract: A semiconductor device package includes a first redistribution layer (RDL), a first die, a second die, a second RDL and an encapsulant. The first die is disposed on the first RDL and is electrically connected to the first RDL. The first die has a first electrical contact. The second die is disposed on the first RDL and is electrically connected to the first RDL. The second die has a first electrical contact. The second RDL is surrounded by the first RDL. The second RDL has a first electrical contact electrically connected to the first electrical contact of the first die and a second electrical contact electrically connected to the first electrical contact of the second die. A size of the first electrical contact of the second RDL is greater than a size of the second electrical contact of the second RDL.

Abstract: A negative electrode active material contains particle of the negative electrode active material, wherein the particle of the negative electrode active material contains particle of a silicon compound which contains a silicon compound (SiOx: 0.5?x?1.6), the particle of the silicon compound contains lithium, and the particle of the negative electrode active material has a total content rate of a polyphenylene compound component and a polycyclic aromatic component measured by TPD-MS of 1 ppm by mass or more and 4,000 ppm by mass or less. As a result, a negative electrode active material is capable of improving cycle characteristics and initial charge/discharge characteristics when it is used as a negative electrode active material of a lithium ion secondary battery.

Abstract: An electrical connector includes an insulation body having a base and a tongue located in a front end of the base, terminals arranged in upper and lower rows, and a middle grounding plate disposed in the base and extending to the tongue. Terminals in the upper and lower rows are arranged on upper and lower surfaces of the tongue, respectively. The middle grounding plate is located between the upper and lower rows of terminals, and having at least one first hole. An edge of each of two lateral sides of the middle grounding plate is inwardly depressed to form a first groove. The first hole is located between the two first grooves. The insulation body has a first insulation post and a second insulation post, the first insulation post protrudes into the first hole, and the second insulation post protrudes into the first grooves on the lateral sides.

Abstract: The invention refers to a novel method of preparing strand-specific RNA-sequencing libraries that can be used to identify DNA coding and non-coding strands that are transcribed mRNA Strand to RNA. Such strand-specific RNA-sequencing libraries are especially useful in discovering anti-sense RNA and non-coding RNA. Random primer oligonucleotides, covalently coupled to a moiety, which blocks ligation, are used for RT reaction or the subsequent generation of the second DNA strand so that only one strand of the generated double-stranded DNA is ligated to sequencing adapters at the 5? nucleotide and sequenced by paired-end sequencing.

Abstract: The objective of the present invention is to provide a mixture paste that is for the production of a negative electrode for a lithium ion secondary battery, that is not prone to cycle deterioration as a result of changes in volume, and that is not prone to decreases in initial charge/discharge efficiency as a result of reduction of a polyimide during charging. This objective is achieved by a mixture paste for a negative electrode of a lithium ion secondary battery comprising: a binder resin composition that comprises a polyamic acid and/or a corresponding polyimide, said polyamic acid being obtained from a diamine compound and a tetracarboxylic acid dianhydride, and said diamine compound comprising a diamine represented by formula (I) or (II); and a negative electrode active substance containing a silicon oxide represented by SiOx (0.5?x?1.5) and carbon particles.

Abstract: The present invention provides new methods and kits to improve the efficiency of ligation reactions, in particular in molecular biology applications, such as the next generation sequencing (NGS) library construction methods. In next-generation sequencing methods, the ligation step is critical in adding sequencing platform-specific adapters to the DNA fragments that are to be sequenced. Said improvement is achieved by the addition of single- or double-stranded DNA-binding proteins in the ligation step.

Abstract: An electrical connector includes an insulation body having a base portion and a tongue located at a front end of the base portion. Multiple terminals are fixed on the base portion and partially exposed from a surface of the tongue. An outer metal casing surrounds the base portion and the tongue and forms an insertion space receiving the tongue. The outer metal casing has a bottom wall and two side walls. Each side wall extends downward to form a stop wall. Each stop wall extends downward to form two soldering pins. The four soldering pins are arranged in two rows. A cushion block is located below the bottom wall and fixed on the two stop walls. Four protruding blocks are formed by protruding downward from a bottom surface of the cushion block. At least two of the protruding blocks are located disposed between the soldering pins in the two rows.

Abstract: An electrical connector, for mating a mating connector having two metal elastic sheets, includes: an insulation body having a base portion and a tongue located at a front end thereof; an upper and lower rows of terminals fixed to the base portion, each terminal having a contact portion exposed from an upper or lower surface of the tongue; a middle shielding sheet, fixed to the base portion and tongue, and located between the two terminal rows; two snap-fit portions disposed at two sides of the middle shielding sheet and exposed from two sides of the tongue, where the two metal elastic sheets buckle the snap-fit portions to prevent disengagement; and an outer metal casing wrapping peripheries of the base portion and tongue. When the mating connector is inserted into the electrical connector after it's assembling, the two snap-fit portions are fixed to the mating connector to ensure stable high-frequency performance.

Abstract: The present invention relates to a method for real-time monitoring and/or quantification of newly-synthesized complementary deoxyribonucleic acid (cDNA) during a reverse transcription reaction of an ribonucleic acid (RNA) template in a sample, the method using a fluorogenic dye binding to RNA:cDNA hybrids. The present invention also relates to the use of this method as well as to kits employing the fluorogenic dye.

Abstract: A semiconductor package device comprises a circuit layer, an electronic component disposed on the circuit layer, a package element and a first encapsulant. The package element is disposed on the circuit layer. The package element includes at least two electrical contacts electrically connected to the circuit layer. The first encapsulant is disposed on the circuit layer. The first encapsulant encapsulates the electronic component and the package element and exposes the electrical contacts of the package element.

Abstract: A semiconductor package structure includes at least one semiconductor die, at least one conductive pillar, an encapsulant and a circuit structure. The semiconductor die has an active surface. The conductive pillar is disposed adjacent to the active surface of the semiconductor die. The encapsulant covers the semiconductor die and the conductive pillar. The encapsulant defines at least one groove adjacent to and surrounding the conductive pillar. The circuit structure is electrically connected to the conductive pillar.

Abstract: The present invention is directed to novel methods and kits to be employed for preparing adapter-ligated amplicons or a sequencing library of a target DNA, respectively.

Abstract: An electrical connector includes an insulation body. Multiple terminals are fixed on the insulation body in an upper row and a lower row. Each terminal has a soldering portion. The soldering portions are arranged in two rows in a front-rear direction. A middle shielding sheet is fixed on the insulation body, and has a planar plate portion. A baffle extends downward from a rear end of the plate portion and is located between the soldering portions in the two rows. The plate portion extends horizontally in a lateral direction to form a connecting portion, which is in front of the rear end of the plate portion. A rear edge of the connecting portion bends downward to form a leg. In the front-rear direction, a distance between the rear end of the plate portion and the rear edge of the connecting portion is greater than a width of the connecting portion.

Abstract: The present invention is directed to methods for the generation of nucleic acids from an RNA template and further nucleic acid replication. Specifically, the invention is directed to the generation and amplification of nucleic acids by reverse transcriptase-polymerase chain reaction (RT-PCR).

Abstract: A mating connector includes a plastic body, which has a main body and an insertion portion formed by extending forward from the main body. A mating space is concavely formed backward from a front end surface of the insertion portion. The insertion portion has a side wall located at a periphery of the mating space and running through the mating space to form at least two through-holes, and the two through-holes do not communicate with each other. A metal cover surrounds the plastic body and is fastened to the main body. A grounding sheet is located between the metal cover and the side wall. The grounding sheet has a body and at least two elastic portions formed by bending forward from the body. The body covers the side wall, and each of the elastic portions is accommodated in a corresponding through-hole and enters the mating space.

Abstract: An electrical connector includes an insulation body. Multiple terminals are arranged in the insulation body in two rows. Each terminal in the lower row has a first retaining portion, a first extending portion bending downward from the first retaining portion, and a first contact portion extending forward from the first retaining portion. A second extending portion is connected to the first extending portion. A middle grounding plate is disposed in the insulation body, having a body portion and a plate portion. The body portion has a first section connected to the plate portion and located above and parallel to the second extending portion, and a second section extending forward from the first section. The plate portion is located at and parallel to the first extending portion. A distance between the first section and the second extending portion is greater than that between the plate portion and the first extending portion.

Abstract: An electrical connector includes an insulation body having a base portion and a tongue located at a front end of the base portion. Multiple terminals are fixed on the base portion and partially exposed from a surface of the tongue. An outer metal casing surrounds the base portion and the tongue and forms an insertion space receiving the tongue. The outer metal casing has a bottom wall and two side walls. Each side wall extends downward to form a stop wall. Each stop wall extends downward to form two soldering pins. The four soldering pins are arranged in two rows. A cushion block is located below the bottom wall and fixed on the two stop walls. Four protruding blocks are formed by protruding downward from a bottom surface of the cushion block. At least two of the protruding blocks are located disposed between the soldering pins in the two rows.

Abstract: An electrical connector includes an insulation body, at least one first terminal and at least one second terminal, and a middle grounding plate. The insulation body has a base and a tongue located in a front end of the base. The first terminal and the second terminal are arranged in the base in an upper row and a lower row, and exposed to an upper surface and a lower surface of the tongue. The middle grounding plate has a body portion fixed to the tongue, and located between the first terminal and the second terminal, the first terminal has a first front edge, the second terminal has a second front edge, and the first front edge, the second front edge and an edge in a front end of the body portion are located in a same vertical plane.