Abstract: The invention relates to a method to increase production of itaconic acid in a micro-organism by inhibiting the expression or functioning of the enzyme itaconyl-CoA transferase, itaconyl-CoA hydratase (citramalyl-CoA hydrolyase; EC 4.2.1.56) and/or citramalyl-CoA lyase. Also embodied are micro-organisms, preferably Aspergillus s terreus or Aspergillus niger, in which said enzyme is inhibited.

Abstract: Closure assembly comprising a spout (1) and a cap (2) including a tamper-evident ring (3). The spout (1) has a neck (14) around a product passage (15) and a wall (10). The wall is spaced from the neck (14) to form an inner space (11) which is open from above to receive the tamper-evident ring (3). The tamper-evident ring (3) is integrally moulded via breakable bridges (34) to the cap and comprises a base portion (32) with at least one snap portion (33) at abase portion outer face (32a). The wall (10) of the spout (1) comprises at least one window (16), wherein the at least one snap portion (33) fits inside the window (16) to snap the tamper-evident ring (3) to the spout (1) when the tamper-evident ring (3) is inserted in the inner space (11).

Abstract: A flow controller for a hot water appliance includes a housing having at least three channels, a branching chamber arranged in the housing and in which the at least three channels debouch, and in which a shut-off valve is arranged with which at least two of the three channels can be closed and left clear. The shut-off valve has an adjustment range with a first extreme position in which a first channel and a second channel of the at least three channels are in flow connection with each other via the branching chamber and in which the first channel and a third channel of the at least three channels are substantially closed off from each other. A hot water appliance can include such a flow controller.

Abstract: The invention relates to a method for enzymatically synthesizing an (oligo)peptide, comprising coupling (a) an (oligo)peptide C-terminal ester or thioester and (b) an (oligo)peptide nucleophile having an N-terminally unprotected amine, wherein the coupling is carried out in a fluid comprising water, and wherein the coupling is catalyzed by a subtilisin BPN? variant or a homolog thereof, which comprises the following mutations compared to subtilisin BPN? represented by SEQUENCE ID NO: 2 or a homolog sequence thereof: a deletion of the amino acids corresponding to positions 75-83; a mutation at the amino acid position corresponding to S221, the mutation being S221C or S221 selenocysteine; preferably a mutation at the amino acid position corresponding to P225 wherein the amino acid positions are defined according to the sequence of subtilisin BPN? represented by SEQUENCE ID NO: 2. Further, the invention relates to an enzyme suitable for use as a catalyst in a method of the invention.

Abstract: The invention relates to a subtilisin BPN? variant or homologue thereof, having the following mutations compared to subtilisin BPN? represented by SEQUENCE ID NO: 2 or a homologue sequence thereof:—a deletion of the amino acids corresponding to positions 75-83;—a mutation at the amino acid position corresponding to S221, the mutation corresponding to S221C or S221 selenocysteine, preferably S221C;—at least one further mutation selected from the group consisting of amino acid positions corresponding to F189W, F189Y, S33D, S33T, N218D, N218T, N218E, N62D, N62S, N62W, and N62Y; preferably a mutation at the amino acid position corresponding to P225; wherein the amino acid positions are defined according to the sequence of subtilisin BPN? represented by SEQUENCE ID NO: 2. The invention further relates to enzymatically synthesizing a peptide.

Abstract: A method of monitoring a bone fracture in an individual. The method comprises the steps of: measuring a first parameter indicative of the load on said bone fracture; measuring a second parameter indicative of the activity level of said individual; obtaining a plurality of values for said first and second parameters; correlating said values to determine a measure of bone fracture condition; monitoring a change in said correlation over time; and determining a measure of the change in bone fracture condition over time. A bone fixation system implements this method of monitoring a bone fracture in an individual. The method and system provide a more accurate and reliable measure of bone fracture condition and of the progression of healing of the bone fracture.

Abstract: A system and computer-implemented method of altering perceptibility of depth in an image. The method comprises receiving a desired change in the perceptibility of depth in the image; receiving a depth-map corresponding to the image; and determining at least one characteristic of the image. The method further comprises applying an image process to the image, the image process varying in strength according to the depth map, and in accordance with a non-linear predetermined mapping relating a strength of the applied image process to a change in the perceptibility of depth, the mapping being determined with respect to the identified characteristic.

Abstract: A hot water appliance includes a housing defining an inner space; a heat source arranged in the inner space of the housing and comprising at least one burner; a flue gas discharge arranged in the inner space of the housing and configured to discharge combustion gases of the at least one burner therethrough; and a heat exchanger arranged in the inner space of the housing and associated with the flue gas discharge. The combustion gases of the at least one burner form a first heat exchanging fluid of the heat exchanger associated with the flue gas discharge. A flue gas discharge for a hot water appliance and a method for heating a fluid are also described.

Abstract: The invention relates to a method for enzymatically synthesizing an (oligo)peptide, comprising coupling (a) an (oligo)peptide C-terminal ester or thioester and (b) an (oligo)peptide nucleophile having an N-terminally unprotected amine, wherein the coupling is carried out in a fluid comprising water, and wherein the coupling is catalyzed by a subtilisin BPN? variant or a homologue thereof, which comprises the following mutations compared to subtilisin BPN? represented by SEQUENCE ID NO: 2 or a homologue sequence thereof : a deletion of the amino acids corresponding to positions 75-83; a mutation at the amino acid position corresponding to S221, the mutation being S221C or S221 selenocysteine; preferably a mutation at the amino acid position corresponding to P225 wherein the amino acid positions are defined according to the sequence of subtilisin BPN? represented by SEQUENCE ID NO: 2. Further, the invention relates to an enzyme suitable for use as a catalyst in a method of the invention.

Abstract: A method for providing pre-assembled and sterilized container closure assemblies that are each adapted to be secured onto a container including manufacturing pre-assembled container closure assemblies each adapted to be secured onto a container, injection molding a monolithic spout body of thermoplastic material, injection molding a manually removable rotational cap, securing the cap onto the neck of the spout body in a closed position of the cap, submerging each of the pre-assembled container closure assemblies in a bath of sterilizing liquid, and removing the container closure assemblies from the bath of sterilizing liquid, and allowing the sterilizing liquid to drain. A pre-assembled container closure assembly adapted to be secured onto a container is also disclosed.

Abstract: A domestic appliance has an operating element, the operating element comprising a carrier, at least one pressure sensor configured for detection of an operation of the operating element, and at least one light source. A control is configured to switch the light source on or off subject to a sensor signal coming from the pressure sensor. At least one light conductor is arranged close to the light source and is configured to conduct light from the light source to at least a side of the light conductor remote from the carrier. Provided on or close to the side of the light conductor remote from the carrier is a screen comprising at least one opaque part and at least one translucent part, which together form a symbol.

Abstract: The invention relates to a method for enzymatically synthesizing an (oligo)peptide, comprising coupling (a) an (oligo)peptide C-terminal ester or thioester and (b) an (oligo)peptide nucleophile having an N-terminally unprotected amine, wherein the coupling is carried out in a fluid comprising water, and wherein the coupling is catalyzed by a subtilisin BPN? variant or a homo-log thereof, which comprises the following mutations compared to subtilisin BPN? represented by SEQUENCE ID NO: 2 or a homolog sequence thereof: a deletion of the amino acids corresponding to positions 75-83; a mutation at the amino acid position corresponding to S221, the mutation being S221C or S221 selenocysteine; preferably a mutation at the amino acid position corresponding to P225 wherein the amino acid positions are defined according to the sequence of subtilisin BPN? represented by SEQUENCE ID NO: 2. Further, the invention relates to an enzyme suitable for use as a catalyst in a method of the invention.

Abstract: The present invention pertains to a method for automatic sea lice monitoring in fish aquaculture, the method comprising submerging a camera (4) in a sea pen (300) comprising fish, using the camera to make an image of at least one of said fish, analysing the image to differentiate between individual sea lice present on the fish and the fish itself and assessing the number of sea lice present on the fish, wherein the camera is attached to a device (1, 10, 100) for guiding the salmon along an imaging track (5), the camera being directed to the track.

Abstract: The invention relates to the use of a cytosolic citric acid synthase for the heterologous production of citrate outside the mitochondrion of a micro-organism or algae, wherein the protein is selected from A. niger An08g10920, An01g09940, An09g03570 ,or an ortholog of these genes. Such production is achieved by introducing the nucleic acid encoding such a protein into a suitable host cell. Preferably the protein is A. niger An08g10920, An01g09940, An09g03570 or an ortholog thereof, more particularly, wherein such an ortholog is chosen from the group of proteins listed in FIG. 9 and proteins having a percentage identity of 70%, more preferably 75%, more preferably 80%, more preferably 85%, more preferably 90%, more preferably 95%, more preferably 98%, more preferably 99% with An08g10920, An01g09940 or An09g03570.

Abstract: Liquid dispensing tap 1 connectable to a liquid container 70, e.g. a bag in box, for controlled dispensing of a liquid therefrom. The tap comprises a housing 10 forming an inlet of the tap and comprising a dispensing chamber wall portion 13 delimiting a dispensing chamber 14 with a valve seat 16 at an axial end and forming a lateral dispensing outlet 17 in communication with the dispensing chamber 14. The tap 1 comprises a plunger 30 with a stem 31 and a valve portion 32, the stem having an actuator engagement portion 33 outside said dispensing chamber and extending along an axis 15 through a bore 18 in the dispensing chamber wall portion and through the dispensing chamber. The plunger moves axially between a closed and an open position. A manually operable plunger actuator 50 engages said engagement portion of the stem to open the tap.

Abstract: A content distribution system is provided comprising a content client and a content server connected through a network. The content server is configured to send the content client a session description comprising at least one media description to enable it to receive over the network a content item. The system comprises a network monitor for monitoring network traffic going through the network, and a recommender for marking in dependency upon the network monitor, one or more of the media descriptions in the session description to obtain a marked session description. The marked media description recommends the content client to prefer certain content above others in view of the current network condition. For example, if available bandwidth is low, content is recommended which requires low bandwidth. Network congestion is thereby avoided for all users of the system.

Abstract: The present invention relates to a centrifugal fan comprising a fan housing with a radial outlet opening arranged in a radial peripheral wall thereof, a rotor arranged rotatably in the fan housing and provided close to an axial rotation axis thereof with a rotor inflow opening, wherein the fan housing is provided close to the rotation axis of the rotor with an axial inlet opening, and a tube part which extends in the fan housing from the radial outlet opening arranged in the radial peripheral wall and inward along the radial peripheral wall. The invention further relates to a heating device comprising a burner with a fuel mixture infeed opening, a centrifugal fan according to the invention, and wherein a fluid connection is provided between the fuel mixture infeed opening of the burner and the outlet opening of the centrifugal fan.

Abstract: The invention further relates to a process for the enzymatic synthesis of an (oligo)peptide. The invention relates to a method for designing an enzymatic synthesis process of an (oligo)peptide, comprising identifying two or more (oligo)peptide fragments of an (oligo)peptide, which fragments are (oligo)peptides suitable for preparing the (oligo)peptide by enzymatic condensation of the two or more peptide fragments using a ligase. The invention relates to a method for designing an enzymatic synthesis process of a cyclic (oligo)peptide, comprising identifying a non-cyclic (oligo)peptide from which the cyclic (oligo)peptide can be prepared by cyclisation, catalysed by a cyclase. The invention further relates to a process for the enzymatic synthesis of an (oligo)peptide.

Abstract: A collapsible pouch (1) is provided with a closure assembly (10; 110; 210; 310; 410), which comprises a monolithic spout base and cap part (11; 111; 311) and a tubular inner part (30; 130; 230; 330; 430). The inner part comprises a fixation portion (31; 131; 331; 431) and a neck portion (32; 132; 232) and has been inserted into the spout base and cap part via the lower opening of the bore thereof in a pre-assembly operation prior to sealing of the pre-assembled closure assembly between the two walls (3, 4) of the pouch. The fixation portion is received and fixed in the bore of the spout base (12; 112; 212). The neck portion protrudes from the spout base into the cap cavity and extends to a mouth opening (34; 134) of the neck portion.