Abstract: The invention discloses a method for increasing the yield of L-arginine by knocking out flavin reductases, and belongs to the technical field of amino acid production by microbial fermentation. Genes frd1 and frd2 for encoding hypothetic NADPH-dependent FMN reductase in Corynebacterium crenatum SDNN403 are over-expressed in E. coli BL21 and are purified to form target proteins Frd181 and Frd188, and functions of the target proteins are identified to obtain a result showing that the proteins Frd181 and Frd188 both are NAD(P)H-dependent flavin reductases producing H2O2. By taking a genome of the Corynebacterium crenatum SDNN403 as a template, frd1 and frd2 gene deletion fragments are obtained by overlap extension PCR; connecting pK18mobsacB to obtain knockout plasmids pK18mobsacB-?frd1 and pK18mobsacB-?frd2; carrying out electric shock to transform the Corynebacterium crenatum SDNN403; and carrying out secondary screening to obtain recombinant strains 403?frd1 and 403?frd2.

Abstract: This invention describes a method of using microbial to produce fats, such as fatty acids and their derivatives, or products derived from the fatty acid synthesis cycle, such as hydroxyfatty acids, methyl ketones, and the like.

Abstract: A water-cooling device includes a liquid reservoir main body having a heat exchange chamber, a pump having a stator and a rotor and a heat exchange component connected with the liquid reservoir main body. The heat exchange chamber is for a cooling liquid to pass through. The rotor is connected with an impeller and exposed to the cooling liquid in the heat exchange chamber. The stator is integrally embedded in the liquid reservoir main body by embedded injection molding. The stator is isolated from the heat exchange chamber. According to the design of the water-cooling device, the structural strength of the liquid reservoir main body is enhanced and a waterproof effect for the stator is achieved.

Abstract: An autonomous driving system for a vehicle includes an autonomous lane change safety check method. An object relative to the vehicle and a current lane of travel is mapped and object data of the object is assessed. An object threat value for the object is assigned based on the object map and the object data. The object is grouped based the object threat value. An overall critical value is determined from the object threat value for the object and filtered to reduce signal noise. The critical value is compared to a predetermined criticality threshold, where the lane change safety check is failed when the critical value is over the threshold.

Abstract: Use of BUBR1 as a biomarker for predicting the response to a compound, preferably resistance of a disease such as cancer in a subject, wherein the compound is a furazanobenzimidazole compound of general formula (I).

Abstract: Disclosed is a method for producing L-citrulline using recombinant Corynebacterium crenatum cells as whole-cell biocatalysts. The present invention provides a recombinant C. crenatum that expresses an exogenous arginine deiminase gene from Lactobacillus brevis. The recombinant C. crenatum SDNN403 is used as biocatalysts for converting L-arginine to produce L-citrulline. Using the method of the invention, the concentration of L-citrulline reached 301.4 g/L after a 48 hr conversion reaction, and the molar conversion rate reached 99.9%.

Abstract: A method and sensor system are disclosed for automatically determining object sensor position and alignment on a host vehicle. A radar sensor detects objects surrounding the host vehicle in normal operation. Static objects are identified as those objects with ground speed approximately equal to zero. Vehicle dynamics sensors provide vehicle longitudinal and lateral velocity and yaw rate data. Measurement data for the static objects—including azimuth angle, range and range rate relative to the sensor—along with the vehicle dynamics data, are used in a recursive geometric calculation which converges on actual values of the radar sensor's two-dimensional position and azimuth alignment angle on the host vehicle.

Abstract: The present invention relates to application of a novel signal peptide in L-arginine and its derivatives production from konjac powder, which belongs to the field of gene engineering, enzyme engineering and metabolism engineering. The present invention fused the signal peptide set forth in SEQ ID NO.1 with the ?-mannanase of Bacillus subtilis CCTCC M 209200, and expressed the fused gene in the strain with high L-arginine yield. The recombinant strain Corynebacterium crenatum CGMCC 0890/p MSPman had advantages on utilizing cheaper konjac powder as substrate, and after fermenting for 96 hours in a 5 L bioreactor, the L-arginine yield reached 45 g/L. Another two recombinant strains were constructed based on Corynebacterium crenatum CGMCC 0890/pMSPman, and after fermenting for 96 hours in a 5 L bioreactor, the L-ornithine yield and L-citrulline reached 23.5 g/L and 26.3 g/L respectively.

Abstract: The present disclosure is applicable to the field of information technologies, and provides a route information displaying method and apparatus. The method includes: creating a three-dimensional coordinate system, and loading an electronic map in the XY plane of the three-dimensional coordinate system; acquiring, by using a satellite positioning system, a real-time location of a mobile terminal and a real-time speed, of the mobile terminal, corresponding to the real-time location; and drawing both a moving route and a speed curve of the mobile terminal in the three-dimensional coordinate system according to the acquired real-time location and real-time speed, where the real-time location of the mobile terminal is represented in the XY plane of the three-dimensional coordinate system, and the real-time speed of the mobile terminal is represented on the Z axis of the three-dimensional coordinate system.

Abstract: The present invention relates to application of a novel signal peptide in L-glutamate and its derivatives production from konjac powder, which belongs to the field of gene engineering, enzyme engineering and metabolism engineering. The signal peptide which mediated secretion of ?-mannanase was invented, and the recombinant strain with this signal peptide had advantages on utilizing konjac powder to produce related products, and its utilization efficiency of konjac powder, production efficiency, and yield were higher than other signal peptides. The recombinant strain possessing this new signal peptide had advantages on utilizing cheaper konjac powder as substrate to lower the process costs on L-glutamic acid and its high-value-added products.

Abstract: The present disclosure is applicable to the field of information technologies, and provides a route information displaying method and apparatus. The method includes: creating a three-dimensional coordinate system, and loading an electronic map in the XY plane of the three-dimensional coordinate system; acquiring, by using a satellite positioning system, a real-time location of a mobile terminal and a real-time speed, of the mobile terminal, corresponding to the real-time location; and drawing both a moving route and a speed curve of the mobile terminal in the three-dimensional coordinate system according to the acquired real-time location and real-time speed, where the real-time location of the mobile terminal is represented in the XY plane of the three-dimensional coordinate system, and the real-time speed of the mobile terminal is represented on the Z axis of the three-dimensional coordinate system.

Abstract: The present disclosure is applicable to the field of information technologies, and provides a route information displaying method and apparatus. The method includes: creating a three-dimensional coordinate system, and loading an electronic map in the XY plane of the three-dimensional coordinate system; acquiring, by using a satellite positioning system, a real-time location of a mobile terminal and a real-time speed, of the mobile terminal, corresponding to the real-time location; and drawing both a moving route and a speed curve of the mobile terminal in the three-dimensional coordinate system according to the acquired real-time location and real-time speed, where the real-time location of the mobile terminal is represented in the XY plane of the three-dimensional coordinate system, and the real-time speed of the mobile terminal is represented on the Z axis of the three-dimensional coordinate system.

Abstract: A method of arbitrating conflicting outputs in a redundant control system. Execution data of a task executed by each controller in the redundant control system is recorded. The execution data includes an initial timestamp of each execution stream, identification of critical functions in each execution stream, and parameter values used by the critical functions. A path executed by each controller is identified based only on the critical functions executed for each execution stream. The recorded execution data of each executed path is applied to an arbitration module. An output result from one of the respective controllers selecting, by an arbitration module, based on the recorded execution data of each executed path. The output result of the selected controller is communicated to a next module for further processing.

Abstract: Disclosed is a method for producing L-citrulline using recombinant Corynebacterium crenatum cells as whole-cell biocatalysts. The present invention provides a recombinant C. crenatum that expresses an exogenous arginine deiminase gene from Lactobacillus brevis. The recombinant C. crenatum SDNN403 is used as biocatalysts for converting L-arginine to produce L-citrulline. Using the method of the invention, the concentration of L-citrulline reached 301.4 g/L after a 48 hr conversion reaction, and the molar conversion rate reached 99.9%.

Abstract: The present disclosure describes a genetically engineered a KASIII-independent fatty acid biosynthetic pathway that makes use of the promiscuous nature of the rest of the FAS enzymes (3-ketoacyl-ACP synthetase, 3-ketoacyl-ACP reductase, 3-hydroxyacyl ACP dehydrase, enoyl-ACP reductase) to bypass the KASIII step by providing a Co-A precursor of two or higher than two carbons (such as the four carbon butyryl-CoA) as the starting molecule. Since many CoA-based starter molecules can be supplied for the fatty acid synthesis, much more diversified products can be obtained with various carbon-chain lengths. As such, this disclosure will serve as a powerful and efficient platform to produce low to medium chain length products carrying many different functional groups.

Abstract: Use of BUBR1 as a biomarker for predicting the response to a compound, preferably resistance of a disease such as cancer in a subject, wherein the compound is a compound of general formula I wherein R represents phenyl, thienyl or pyridinyl wherein phenyl is optionally substituted by one or two substituents independently selected from alkyl, halo-lower alkyl, hydroxy-lower alkyl, lower alkoxy-lower alkyl, acyloxy-lower alkyl, phenyl, hydroxy, lower alkoxy, hydroxy-lower alkoxy, lower alkoxy-lower alkoxy, phenyl-lower alkoxy, lower alkylcarbonyloxy, amino, monoalkylamino, dialkylamino, lower alkoxycarbonylamino, lower alkylcarbonylamino, substituted amino wherein the two substituents on nitrogen form together with the nitrogen heterocyclyl, lower alkylcarbonyl, carboxy, lower alkoxycarbonyl, cyano, halogen, and nitro; and wherein two adjacent substituents are methylenedioxy; and wherein pyridinyl is optionally substituted by lower alkoxy, amino or halogen; X represents a group C?Y, wherein Y stands for ox

Abstract: The present disclosure is applicable to the field of information technologies, and provides a route information displaying method and apparatus. The method includes: creating a three-dimensional coordinate system, and loading an electronic map in the XY plane of the three-dimensional coordinate system; acquiring, by using a satellite positioning system, a real-time location of a mobile terminal and a real-time speed, of the mobile terminal, corresponding to the real-time location; and drawing both a moving route and a speed curve of the mobile terminal in the three-dimensional coordinate system according to the acquired real-time location and real-time speed, where the real-time location of the mobile terminal is represented in the XY plane of the three-dimensional coordinate system, and the real-time speed of the mobile terminal is represented on the Z axis of the three-dimensional coordinate system.

Abstract: A novel nucleoside phosphoramidate compound of the formula below, or a stereoisomer, salt, hydrate, solvate or crystal thereof for the treatment of Flaviviridae family viral infection, especially hepatitis C viral infection, and having a good anti-HCV effect; a pharmaceutical composition having the compound, or a stereoisomer, salt, hydrate, solvate or crystal thereof:

Abstract: A method for monitoring performance of a navigation system onboard a vehicle is provided. The method obtains, at a vehicle electronic control unit (ECU), a triangulated vehicle location and vehicle motion data, the triangulated vehicle location obtained from the navigation system; computes an estimated vehicle location, using the triangulated vehicle location and the vehicle motion data; calculates a probability that the estimated vehicle location exists within an error bound of the triangulated vehicle location; and when the probability indicates that the estimated vehicle location does not exist within the error bound, performs a task onboard the vehicle.

Abstract: The present disclosure is applicable to the field of information technologies, and provides a route information displaying method and apparatus. The method includes: creating a three-dimensional coordinate system, and loading an electronic map in the XY plane of the three-dimensional coordinate system; acquiring, by using a satellite positioning system, a real-time location of a mobile terminal and a real-time speed, of the mobile terminal, corresponding to the real-time location; and drawing both a moving route and a speed curve of the mobile terminal in the three-dimensional coordinate system according to the acquired real-time location and real-time speed, where the real-time location of the mobile terminal is represented in the XY plane of the three-dimensional coordinate system, and the real-time speed of the mobile terminal is represented on the Z axis of the three-dimensional coordinate system.