Patents Issued in July 15, 2014
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Patent number: 8778640Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: July 15, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8778641Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: July 15, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8778642Abstract: Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid.Type: GrantFiled: June 4, 2012Date of Patent: July 15, 2014Assignee: Celexion, LLCInventors: Brian M. Baynes, John Michael Geremia
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Patent number: 8778643Abstract: The present disclosure relates to methods of increasing lipid levels in an algal or yeast cell population, methods of producing saturated or monounsaturated triacylglycerols in an algal or yeast cell population, and methods of decreasing polyunsaturated triacylglycerol production in an algal or yeast cell population by contacting the cell population with a chemical compound that is capable of increasing lipid levels or altering the lipid composition in the cell population.Type: GrantFiled: March 13, 2013Date of Patent: July 15, 2014Assignee: The Regents of the University of CaliforniaInventors: Annaliese Kirsten Franz, Megan Danielewicz, Diana Meiying Wong, Lisa Adele Anderson
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Patent number: 8778644Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.Type: GrantFiled: September 16, 2013Date of Patent: July 15, 2014Assignee: Commonwealth Scientific and Industrial Research OrganisationInventors: Surinder Pal Singh, Stanley Suresh Robert, Peter David Nichols, Susan Irene Ellis Blackburn, Xue-Rong Zhou, James Robertson Petrie, Allan Graham Green
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Patent number: 8778645Abstract: The present invention relates to a novel method for the biocatalytic production of unsaturated dicarboxylic acids by cultivating a recombinant microorganism co-expressing a glutaconate CoA-transferase and a 2-hydroxyglutaryl-CoA dehydratase system. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as a method of preparing polyamide or polyester copolymers making use of said dicarboxylic acids as obtained by said biocatalytic production method.Type: GrantFiled: January 15, 2010Date of Patent: July 15, 2014Assignee: BASF SEInventors: Oskar Zelder, Wolfgang Buckel, Ivana Djurdjevic
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Patent number: 8778646Abstract: A method of reducing undesirable microorganism concentration, the method comprises (a) introducing a quantity of fermentable carbohydrate, sugar or cellulose to an aqueous system, (b) introducing a quantity of desirable microorganism to the aqueous system, (c) introducing a hops acid extract into the aqueous system and (d) introducing an organic acid solution into said system.Type: GrantFiled: March 15, 2013Date of Patent: July 15, 2014Assignee: Hercules IncorporatedInventors: John S. Chapman, Corinne E. Consalo
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Patent number: 8778647Abstract: The invention provides improved methods for the production of isoprene from biological materials using a membrane bioreactor to culture isoprene-producing cells.Type: GrantFiled: December 22, 2010Date of Patent: July 15, 2014Assignees: Danisco US Inc., The Goodyear Tire & Rubber CompanyInventors: Anthony R. Calabria, Gopal K. Chotani, Robin Fong, Alex T. Nielsen, Karl J. Sanford
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Patent number: 8778648Abstract: Compositions suitable for oral administration to an animal comprising at least one immune stress-reducing enzyme in an amount effective to decrease the level of positive acute phase protein in an animal, increase the level of negative acute phase protein in an animal, and/or improve animal growth performance is provided, as are methods using such compositions. The compositions include animal feed compositions, liquid compositions other than animal feed, and solid compositions other than animal feed.Type: GrantFiled: March 25, 2011Date of Patent: July 15, 2014Assignee: Eli Lilly and CompanyInventors: David M. Anderson, Humg-Yu Hsiao, Lin Liu
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Patent number: 8778649Abstract: A thermophilic endo-beta-1,4-xylanase derived from Acidothermus cellulolyticus is disclosed. The xylanase exhibits xylanase activity at an optimal temperature of 90° C. and an optimal pH range of about 4.5-6.0. The isolated xylanase is useful in the hydrolysis of lignocellulosic material.Type: GrantFiled: October 16, 2012Date of Patent: July 15, 2014Assignee: The Regents of the University of CaliforniaInventors: Rebecca E. Parales, Alison M. Berry, Juanito V. Parales, Jr., Ravi D. Barabote
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Patent number: 8778650Abstract: The invention is directed to an alkaline protease that has the amino acid sequence of SEQ ID NO: 2 or of an amino acid sequence having an identity of 90% or more thereto, except that one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of SEQ ID NO: 2, or at positions corresponding thereto, are substituted with certain other amino acid residues. The invention is also directed to detergent compositions and culture medium that contain the protease.Type: GrantFiled: April 26, 2010Date of Patent: July 15, 2014Assignee: Kao CorporationInventors: Masatoshi Tohata, Mitsuyoshi Okuda, Tsuyoshi Sato, Keiji Endo, Katsuhisa Saeki
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Patent number: 8778651Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.Type: GrantFiled: November 12, 2012Date of Patent: July 15, 2014Assignee: Verenium CorporationInventors: Kelvin Wong, Jay M. Short, Mark Burk, Grace DeSantis, Robert Farwell, Kelly Chatman
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Patent number: 8778652Abstract: The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.Type: GrantFiled: June 22, 2012Date of Patent: July 15, 2014Assignee: Codexis, Inc.Inventors: Ezhilkani Subbian, John H. Grate, Catherine M. Cho, Benjamin Mijts, Jeanne Bonomo Benoit
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Patent number: 8778653Abstract: A method for purification of viral compositions is provided. In particular, a method for reduction of unwanted residual DNA in a viral composition while retaining the immunogenicity of the virus itself is provided. The resulting immunogenic viral composition is substantially free of residual DNA, and is useful for the manufacture of medical products, such as vaccines designed for human or animal.Type: GrantFiled: August 12, 2010Date of Patent: July 15, 2014Assignee: Yisheng Biopharma Holdings Ltd.Inventors: Yi Zhang, Jinming Dai, Yajin Ni
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Patent number: 8778654Abstract: Recombinant bacteria for producing deoxyviolacein and uses thereof are provided, wherein the recombinant bacteria is obtained by introducing the deoxyviolacein synthesis-related gene cluster into Escherichia coli BL21-CodonPlus (DE3)-RIL or Pseudomonas putida mt-2. The deoxyviolacein synthesis-related gene cluster is obtained by knocking out VioD gene from the violacein synthesis-related gene cluster composed of VioA, VioB, VioC, VioD and VioE, and the nucleotide sequence is as shown in the SEQ ID NO: 1 in the sequence listing. A method for producing deoxyviolacein by fermenting the recombinant bacteria to produce deoxyviolacein by using L-tryptophan as substrate is provided. The method has high efficiency of deoxyviolacein production, the deoxyviolacein produced is convenient to be extracted, and simple to be separated and purified.Type: GrantFiled: April 22, 2009Date of Patent: July 15, 2014Assignee: Tsinghua UniversityInventors: Xinhui Xing, Peixia Jiang
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Patent number: 8778655Abstract: The invention relates to Gram-negative bacteria carrying an inactivated gene encoding a glycosyltransferase involved in the synthesis of the core of the LPS of said Gram-negative bacteria, wherein said inactivated gene results in the synthesis of a LPS having a modified core. These strains have an attenuated virulence but induce a humoral immunity sufficient for ensuring vaccination of the host.Type: GrantFiled: September 21, 2010Date of Patent: July 15, 2014Assignees: Institut National de la Sante et de la Recherche Medicale (INSERM), Centre National de la Recherche Scientifique—CNRS, Universite de la Mediterranee—Aix-Marseille II, Universidad de NavarraInventors: Jean-Pierre Gorvel, Vilma Arce Gorvel, Maite Iriarte, Ignacio Moriyon, Raquel Conde-Alvarez
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Patent number: 8778656Abstract: This invention relates to succinic acid production from renewable feedstock using microbial biocatalysts genetically modified to produce succinic acid in commercially significant quantities. More specifically, this invention relates to the genetic manipulations in the pathway of carbon from renewable feedstock to succinic acid.Type: GrantFiled: November 18, 2010Date of Patent: July 15, 2014Assignee: Myriant CorporationInventors: Sudhanshu Dole, R. Rogers Yocum
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Patent number: 8778657Abstract: The present invention discloses culture medium unit doses for cultivating microorganisms comprising at least two compositions, each composition packaged in a composition unit dose of a predetermined amount, the composition unit doses being used for combining one of each composition unit dose forming the culture medium unit dose. The composition unit doses being packaged separately and individually until a time the culture medium unit dose is to be prepared for use for cultivation of microorganisms, wherein the time one of each composition unit dose are combined thereby forming the culture medium unit dose. The invention also discloses a method of manufacturing the composition unit doses, and a kit for cultivating microorganisms, the kit comprising a combination of the composition unit doses.Type: GrantFiled: October 17, 2012Date of Patent: July 15, 2014Assignee: Hardy DiagnosticsInventors: Gary A. Peterson, Andre Y. Hsiung
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Patent number: 8778658Abstract: The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production.Type: GrantFiled: July 13, 2012Date of Patent: July 15, 2014Assignee: Verdezyne, Inc.Inventors: Stephen Picataggio, Tom Beardslee
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Patent number: 8778659Abstract: The disclosure relates to method of reducing O-glycosylation levels of the insulin or insulin analog precursor molecule produced by Pichia sp. The present disclosure provides genetically engineered knock-out strains of methylotrophic yeast including Pichia and especially Pichia pastoris by disruption of Protein mannosyl transferase (PMT) genes and rendering them capable of producing heterologous proteins with reduced glycosylation. Vectors, which comprise coding sequences for PMT1, PMT2, PMT4, PMT5, and PMT6 genes, for transforming methylotrophic yeasts are contemplated by the present disclosure. PMT inactivated strains of this disclosure have been deposited at MTCC, Chandigarh. The strains are PMT1/GS115 (MTCC 5515), PMT4/GS115 (MTCC 5516), PMT5/GS115 (MTCC 5517) and PMT6/GS115 (MTCC 5518).Type: GrantFiled: March 29, 2010Date of Patent: July 15, 2014Assignee: Biocon LimitedInventors: Nagaraj Govindappa, Komal Kanojia, Krishnamurthy Venkatesan, Nitesh Dave, Mukesh Babuappa Patale, Sanjay Tiwari, Kedarnath N. Sastry, Harish Iyer
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Patent number: 8778660Abstract: A method of increasing yield of cultivated algae, which entails the step of cultivating one or more species of algae in the presence of one or more species of PPFM bacteria, for at least a portion of the algae cultivation.Type: GrantFiled: April 22, 2011Date of Patent: July 15, 2014Assignee: NewLeaf Symbiotics, Inc.Inventors: Mark Holland, Patrick Di Bello, Richard M. Carlton
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Patent number: 8778661Abstract: A biological composite and method for reducing H2S are disclosed. The biological composite for reducing H2S includes a carrier and Thermus sp. immobilized on the carrier. Therefore, if a sample containing H2S has contact with Thermus sp. or the carrier with Thermus sp. immobilized thereon, the amount of H2S can be reduced.Type: GrantFiled: March 12, 2012Date of Patent: July 15, 2014Assignee: National Chiao Tung UniversityInventors: Ching-Ping Tseng, Yu-Pei Chen
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Patent number: 8778662Abstract: Disclosed is a method for removing endotoxin from proteins. Also disclosed are products made by using the method. The method may be used, for example, to produce endotoxin-free lactoferrin. Bovine milk-derived lactoferrin may be produced in commercial quantities by the method, and endotoxin-free bovine lactoferrin may be used for a variety of therapeutic uses, including improving wound healing.Type: GrantFiled: March 19, 2012Date of Patent: July 15, 2014Assignee: Glanbia Nutritionals (Ireland) PLCInventors: Loren S. Ward, Kevin Thomson, Stanley Wroby
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Patent number: 8778663Abstract: A thermalcycler includes a first thermalcycler body section having a first face and a second thermalcycler body section having a second face. A cavity is formed by the first face and the second face. A thermalcycling unit is positioned in the cavity. A heater trace unit is connected to a support section, to the first thermalcycler body section, to the second thermalcycler body section, and to the thermalcycling unit. The first thermalcycler body section and the second thermalcycler body section are positioned together against the support section to enclose the thermalcycling unit and the heater trace unit.Type: GrantFiled: September 18, 2007Date of Patent: July 15, 2014Assignee: Lawrence Livermore National Security, LLC.Inventors: William J. Benett, John T. Andreski, John M. Dzenitis, Anthony J. Makarewicz, Dean R. Hadley, Satinderpall S. Pannu
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Patent number: 8778664Abstract: A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof.Type: GrantFiled: June 19, 2012Date of Patent: July 15, 2014Assignee: Canon Kabushiki KaishaInventors: Nobuhiro Tomatsu, Toshifumi Fukui, Nobuyoshi Shimizu, Atsushi Takayanagi
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Patent number: 8778665Abstract: This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications.Type: GrantFiled: March 30, 2010Date of Patent: July 15, 2014Assignee: Theranos, Inc.Inventors: Ian Gibbons, Shaunak Roy, Edmond Ku
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Patent number: 8778666Abstract: A device comprises: one or more cantilevered biomimetic cilia, and a liquid disposed among the one or more biomimetic cilia, wherein individual biomimetic cilia are at least partially submerged in the liquid, and wherein the biomimetic cilia are arranged for excitation into resonance, such as for mixing and pumping via the resonant behavior of the excited cilia.Type: GrantFiled: October 27, 2009Date of Patent: July 15, 2014Assignee: University of WashingtonInventors: Jae Chung, Santosh Devasia, James J. Riley, Kieseok Oh, Kyong Hoon Lee, Jiradech Kongthon
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Patent number: 8778667Abstract: The present invention relates to a unit to be used for detection of interactions of biologically relevant molecules using a carrier on which the biologically relevant molecules are immobilized, comprising: a hollow holder having an open part at one end with the other end being closed; and a carrier-supporting member that can be inserted into the hollow holder, on which is mounted a carrier upon which biologically relevant molecules are immobilized, wherein: while the carrier-supporting member is being inserted into the hollow holder, a rear-end portion of the carrier-supporting member is engaged with an edge of the open part of the hollow holder, so that the hollow holder is sealed and the positions of the carrier-supporting member and the hollow holder are determined; and the area on the left and the area on the right of the axial center, which are defined by the inner side of the hollow holder and the external side of the carrier-supporting member on which the carrier has been mounted, are approximately thType: GrantFiled: May 26, 2009Date of Patent: July 15, 2014Assignee: Toyo Kohan Co., Ltd.Inventors: Hirofumi Yamano, Shuuichi Kamei, Michifumi Tanga
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Patent number: 8778668Abstract: The present invention provides a modular ethanol production plant constructed of a number of identically sized modules each having a supporting structure main framework to which the components contained in the modules are attached. The plurality of modules includes a fermentation module, a distillation module, a ground grain module, or a sugar source feedstock module, and an optional heating module. Each of the modules are sized to occupy the same approximate space as a standard sized ocean going shipping container. Also, each of the modules has a central walkway including piping and electrical control boxes that are aligned for each connection to the next adjacent module.Type: GrantFiled: January 13, 2012Date of Patent: July 15, 2014Inventor: Mark K. Gaalswyk
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Patent number: 8778669Abstract: The present invention discloses a vessel for culturing cells which includes: a bottom including a base with an upwardly extending wall at least partially bounding the base of the bottom; a top including a base with a downwardly extending wall at least partially bounding the base of the top; a tubular neck with an opening defined therein; and, one or more shelves, wherein, each shelf includes a base with an upwardly extending wall at least partially bounding the base of the shelf. The upwardly extending wall of a first shelf adjoins the downwardly extending wall of the top with the first shelf being located intermediate the bottom and the top. The base of each of the shelves having at least one aperture formed therein. The bottom, the top and the one or more shelves collectively define an enclosed volume for culturing cells. The tubular neck extends from the vessel with the enclosed volume being accessible by the opening in the tubular neck.Type: GrantFiled: July 21, 2010Date of Patent: July 15, 2014Assignee: Corning IncorporatedInventors: William J. Lacey, Michael S. Shanler, Amy W. Cai
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Patent number: 8778670Abstract: A method and apparatus for embedding cells that utilizes a flow-through embedding technique. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway. The apparatus produces an embedded cell block having concentrated cells near the plane of the block to be sectioned in a quick and efficient manner.Type: GrantFiled: September 22, 2011Date of Patent: July 15, 2014Assignee: University of MassachusettsInventor: Andrew H. Fischer
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Patent number: 8778671Abstract: The present invention relates to attenuated, immunogenic West Nile virus chimeras built on a dengue virus backbone for the production of immunogenic, live, attenuated West Nile virus vaccines.Type: GrantFiled: June 18, 2004Date of Patent: July 15, 2014Assignees: The United States of America, as represented by the Secretary of the Department of Health & Human Services, The Walter Reed Army Institute of ResearchInventors: Alexander G. Pletnev, Joseph R. Putnak, Robert M. Chanock, Brian R. Murphy, Stephen S. Whitehead, Joseph E. Blaney, Jr.
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Patent number: 8778672Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for an Arabidopsis thaliana nucellar 1/inner integument (AT NUC1) promoter. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.Type: GrantFiled: October 26, 2010Date of Patent: July 15, 2014Assignee: Pioneer Hi Bred International IncInventors: Shai Lawit, Mark Chamberlin
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Patent number: 8778673Abstract: The present invention generally relates to a method for seeding cells on to a support. In particular, the method relates to a method for seeding cells onto a porous hydrophobic support. The method utilizes centrifugal forces to uniformly guide cell seeding into the support with no loss in viability.Type: GrantFiled: August 11, 2011Date of Patent: July 15, 2014Assignee: Lifescan, Inc.Inventors: Alireza Rezania, Ragae Ghabrial
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Patent number: 8778674Abstract: Embodiments of the present invention include the use of placental alkaline phosphatase alone or in combination with human transferrin and, optionally, human ?1-antitrypsin to enhance the proliferation and survival of transplanted stem cells and stem cell-derived progenitor cells.Type: GrantFiled: February 8, 2013Date of Patent: July 15, 2014Assignee: Zoltan Laboratories LLCInventor: Zoltan Kiss
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Patent number: 8778675Abstract: The present invention relates to specific immortalized avian cell lines expressing telomerase reverse transcriptase (TERT), and exhibiting distinct biologics production patterns. More particularly, the present invention relates to immortalized avian cell line capable of either amplifying Flaviviridae but not capable of amplifying Vaccinia virus strain Copenhagen (VV-COP) nor Modified Vaccinia virus Ankara (MVA), or capable of amplifying both Flaviviridae and Poxviridae. The invention further relates to the use of said immortalized avian cell lines and related methods for producing biologics, including viruses and proteins.Type: GrantFiled: February 25, 2013Date of Patent: July 15, 2014Assignee: Transgene S.A.Inventors: Jean-Marc Balloul, Marina Kapfer, Thierry Menguy
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Patent number: 8778676Abstract: MicroRNA genes are highly associated with chromosomal features involved in the etiology of different cancers. The perturbations in the genomic structure or chromosomal architecture of a cell caused by these cancer-associated chromosomal features can affect the expression of the miR gene(s) located in close proximity to that chromosomal feature. Evaluation of miR gene expression can therefore be used to indicate the presence of a cancer-causing chromosomal lesion in a subject. As the change in miR gene expression level caused by a cancer-associated chromosomal feature may also contribute to cancerigenesis, a given cancer can be treated by restoring the level of miR gene expression to normal. microRNA expression profiling can be used to diagnose cancer and predict whether a particular cancer is associated with an adverse prognosis. The identification of specific mutations associated with genomic regions that harbor miR genes in CLL patients provides a means for diagnosing CLL and possibly other cancers.Type: GrantFiled: August 21, 2013Date of Patent: July 15, 2014Assignee: Thomas Jefferson UniversityInventors: Carlo M. Croce, Chang-Gong Liu, George A. Calin, Cinzia Sevignani
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Patent number: 8778677Abstract: The present invention relates to hybrid cells and methods for producing hybrid cells. In particular, the invention relates to hybrid cells generated from the hybridization of at least three cells where at least two cells are derived from different lineages. The invention further relates to the use of hybrid cells for the expression of proteins useful in a range of diagnostic, prophylactic, therapeutic and/or research applications.Type: GrantFiled: June 10, 2010Date of Patent: July 15, 2014Assignee: BTS Research International PTY. Ltd.Inventors: Galina Kaseko, Tohsak L. Mahaworasilpa
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Patent number: 8778678Abstract: The composition of activated CD4 cells is derived from a healthy human donor. The composition from the healthy human donor is suspended in an infusion media and packaged in a vehicle for administration to a subject to treat disease.Type: GrantFiled: September 21, 2010Date of Patent: July 15, 2014Assignee: Immunovative Therapies Ltd.Inventor: Michael Har-Noy
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Patent number: 8778679Abstract: Human umbilical cord lining stem cells that are capable of differentiating into cells of the mesodermal lineage and ectodermal lineage are described, as well as methods of isolating, expanding, culturing, and cryopreserving such cells.Type: GrantFiled: November 2, 2012Date of Patent: July 15, 2014Assignee: DaVinci Biosciences LLCInventors: Francisco J. Silva, Rafael Gonzalez
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Patent number: 8778680Abstract: A method of differentiating adult stem cells, such as those derived from a teratocarcinoma cell line, the Ntera2/D1 clone (NT2). The developed cells exhibit a stable neurotransmitter phenotype without the required use of growth factors or retinoic acid in differentiation process, which may be difficult to completely remove during commercial production. An identification of specific neurotransmitters is possible in these differentiated NT2-derived neurons (NT2-N) after 30 days in culture or 30 days survival in vivo. The invention includes a method to stably differentiate neuronal stem/precursor cells to a neuronal phenotype for use in cell replacement therapy for neurodegenerative disease, stroke or spinal cord injury. At least four different types of neurons are produced from this method of differentiation: dopaminergic, cholinergic, GABAergic and glutaminergic.Type: GrantFiled: December 28, 2007Date of Patent: July 15, 2014Assignee: University of South FloridaInventors: Samuel Saporta, Elise Spencer, Rania Shamekh
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Patent number: 8778681Abstract: It is an object of the present invention to provide a method for efficient differentiation into blood cells. Specifically, in the present invention, when blood cells are produced from pluripotent stem cells such as ES cells or iPS cells in vitro, the cells are cultured under a low oxygen partial pressure to increase the efficiency of differentiating the pluripotent stem cells into hematopoietic progenitor cells, erythroid progenitor cells, and the like, so as to increase the number of finally obtained, desired blood cells.Type: GrantFiled: March 8, 2013Date of Patent: July 15, 2014Assignees: University of Tokyo, Terumo Kabushiki KaishaInventors: Shinya Sano, Koji Eto, Naoya Takayama, Hiromitsu Nakauchi
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Patent number: 8778682Abstract: A method of delivering exogenous molecules, comprising: providing a plurality of cells having a cell membrane; adding a plurality of exogenous molecules to the cells; exposing the cells to a defocused infrared (IR) light to permeabilize the cell membrane of the cells; and delivering the exogenous molecules to the cells through the permeablized cell membrane, wherein an intensity of the IR light at the optical focus is at least greater than or equal to an order of 104 W/cm2.Type: GrantFiled: August 30, 2011Date of Patent: July 15, 2014Assignee: General Electric CompanyInventors: Vasile Bogdan Neculaes, Kenneth Roger Conway, Allen Lawrence Garner, Evelina Roxana Loghin, Siavash Yazdanfar, Dmitry Vladimirovich Dylov, Brian Michael Davis, Chulmin Joo
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Patent number: 8778683Abstract: The present disclosure provides temperature sensitive essential nucleic acid molecules from a psychrophilic bacterium, proteins encoded by the nucleic acid molecules, as well as recombinant cells into which have been introduced such nucleic acid molecules. The disclosed recombinant cells containing one or more essential nucleic acid molecules from a psychrophilic bacterium are thereby made temperature sensitive, and can be administered to a mammal to induce an immune response in the mammal.Type: GrantFiled: October 7, 2010Date of Patent: July 15, 2014Assignee: UVic Industry Partnerships Inc.Inventor: Francis E. Nano
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Patent number: 8778684Abstract: There is provided an HSV complex which comprises an avirulent HSV and a targeting agent which allows the HSV particle to infect and lyse a specific targeted cell. The inventors have found a way in which avirulent HSV can be targeted to disease cells, e.g. cancer cells, by incorporating an antibody binding domain into one or more viral glycoproteins.Type: GrantFiled: April 13, 2011Date of Patent: July 15, 2014Assignee: Virttu Biologics LimitedInventors: Susanne Moira Brown, Joe Conner
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Patent number: 8778685Abstract: The present invention provides dual labeled protein standards useful for the simultaneous determination of the molecular weight of a subject protein as well as the relative mass (i.e., amount) of the subject protein present in an electrophoresis lane. The invention is also directed to methods suitable for the preparation of such dual labeled protein standards and to methods of using such dual labeled proteins to simultaneously determine the molecular weight and the relative amount of a subject protein. Further embodiments are directed to the use dual labeled protein standards to make a more accurate determination of the amount of a protein present in an electrophoresis lane. Yet further embodiments are directed to kits containing the presently described dual protein standards. Dual labeled protein standards made and used in accordance with the embodiments set forth herein may be used to simultaneously determine the molecular weight and the relative amount of a subject protein in real time.Type: GrantFiled: August 25, 2010Date of Patent: July 15, 2014Assignee: Life Technologies CorporationInventors: Thomas Diller, Timothy Updyke
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Patent number: 8778686Abstract: Provided are an automatic analyzer which moves a dispensing apparatus having a dispensing probe, or a vessel by a driving mechanism to dispense plural liquids into the vessel via the dispensing probe, and measures and analyzes optical characteristic of a reaction liquid in which each of the dispensed liquids is reacted, and a dispensing method thereof. The automatic analyzer includes a drive controlling unit which controls the driving mechanism so that the liquid surface of the liquid and a distal end of the dispensing probe are moved relatively to each other in a vertical direction, and, when the liquids including precipitated substance are to be dispensed, the dispensing probe sucks in the liquids from plural different positions along the vertical direction.Type: GrantFiled: December 29, 2008Date of Patent: July 15, 2014Assignee: Beckman Coulter, Inc.Inventor: Akihisa Kuroda
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Patent number: 8778687Abstract: A method and apparatus for determining the hematocrit of a blood sample disposed within an analysis chamber, the method including the steps of: a) imaging at least a portion of the sample that contains one or more red blood cells and one or more areas void of red blood cells; b) determining a representative optical density value for a plurality of image units optically aligned with portions of the red blood cells, and assigning an optical density first boundary value to those image units; c) determining a representative optical density value of a plurality of image units optically aligned with the one or more regions of the sample devoid of red blood cells, and assigning a second optical density boundary value to those image units; and d) determining the hematocrit of the sample.Type: GrantFiled: January 28, 2013Date of Patent: July 15, 2014Assignee: Abbott Point of Care, Inc.Inventors: Robert A. Levine, Stephen C. Wardlaw, Darryn Unfricht, Niten V. Lalpuria
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Patent number: 8778688Abstract: Described herein are high-throughput methods of monitoring D-serine levels in plasma. The assay involves the use of strong cation solid phase extraction (SPE) to isolate D-serine from plasma, followed by quantitation of D-serine using the D-amino acid oxidase- (DAAO-) catalyzed reaction. Also described are methods of screening for compounds that act as DAAO inhibitors.Type: GrantFiled: November 8, 2012Date of Patent: July 15, 2014Assignee: The Johns Hopkins UniversityInventors: Barbara S. Slusher, Jesse Alt, Camilo Rojas, Takashi Tsukamoto
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Patent number: 8778689Abstract: We disclose the synthesis and use of chiral ionic liquids based on a substituted pentose, furanose, hexose, or pyranose sugar. The compounds and processes to make or use them are provided.Type: GrantFiled: May 23, 2008Date of Patent: July 15, 2014Assignee: The University of the West IndiesInventors: Gurdial Singh, Patrice G. J. Plaza, Bhoomendra A. Bhongade