Patents Issued in August 11, 2016
  • Generation of Lineage-Restricted Progenitor Cells from Differentiated Cells
    Publication number: 20160230144
    Abstract: Method for reprogramming differentiated cells into lineage restricted progenitor cells is provided. The method may include contacting differentiated cells with inhibitors of tyrosine phosphatases and apoptosis to de-differentiate differentiated cells into lineage restricted progenitor cells.
    Type: Application
    Filed: September 28, 2015
    Publication date: August 11, 2016
    Inventors: Irina M. Conboy, Preeti Paliwal
  • DEVICE AND SURGICAL SYSTEM FOR RAPID ASEPTIC ISOLATION AND CONCENTRATION OF AUTOLOGOUS FAT DERIVED STEM CELLS
    Publication number: 20160230145
    Abstract: The present disclosure provides an apparatus for harvesting stem cells from fat tissue. The apparatus may include a first transducer coupled to a first end of an resonant horn to form an ultrasonic resonator. The resonant horn may include an elongated body having a plurality of through-holes configured to accommodate a plurality of specimen containers that are positioned substantially perpendicular to the elongated body. The apparatus may also include a wave generator coupled to the first transducer to generate an ultrasonic wave, wherein the elongated body has a length that is multiples of the half-wavelength of the ultrasonic wave.
    Type: Application
    Filed: February 5, 2016
    Publication date: August 11, 2016
    Inventor: Julian Henley
  • METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELL FROM MESENCHYMAL STEM CELL AND INDUCED PLURIPOTENT STEM CELL PRODUCED BY THE METHOD
    Publication number: 20160230146
    Abstract: The present invention relates to a medium composition containing an Ecklonia cava extract for dediffentiating an induced pluripotent stem cell. Also, the present invention relates to a method for producing induced pluripotent stem cells using the medium composition. When using the medium composition, according to the present invention, induced pluripotent stem cells using mesenchymal stem cells can be produced safely, easily, and efficiently, and the pluripotent stem cells which have been produced can be useful as a cell treatment agent by being capable of being differentiated into a variety of cells.
    Type: Application
    Filed: November 1, 2013
    Publication date: August 11, 2016
    Inventors: Sang Yeon LEE, Won Ju JUNG, Ho Bin KIM, Min Sun OH, Kye Ho LEE
  • METHOD FOR PRODUCING ENVELOPED VIRUSES
    Publication number: 20160230147
    Abstract: The invention relates to a process for producing enveloped viruses in a mildly acid medium. The processes of the invention are useful for producing and recovering at a large scale enveloped viruses under conditions observing good manufacturing practices (or GMP).
    Type: Application
    Filed: September 12, 2014
    Publication date: August 11, 2016
    Applicant: GENETHON
    Inventor: DAVID FENARD
  • PRODUCTION OF VIRUS OCCLUSION BODIES THAT OCCLUDE VIRIONS COMPRISING GENOMES OF DIFFERENT SPECIES OF BACULOVIRUSES THAT CAN BE USED TO COMBAT INSECT PESTS
    Publication number: 20160230148
    Abstract: Production of virus occlusion bodies that occlude virions comprising genomes of different species of baculoviruses that can be used to combat insect pests. A method is presented for the production of occlusion derived virions (ODVs) that simultaneously comprise genomes of different baculovirus species, occluded in a viral occlusion body (OB) with the structural and morphological features characteristic of baculoviruses. Mixed genome ODVs and OBs can be produced by co-infecting insect cells or insect hosts using two or more different baculoviruses species. Co-infection may be achieved by simultaneous inoculation of the different baculoviruses or with a time interval between inoculations, which results in different proportions of each species' genomes in the ODVs and OBs that are produced. The produced OBs can be used either directly for preparing an insecticide, or to infect susceptible insects to produce larger quantities of mixed genome ODVs and OBs, also useful for combating pest insects.
    Type: Application
    Filed: September 23, 2013
    Publication date: August 11, 2016
    Applicants: UNIVERSIDAD PÚBLICA DE NAVARRA, CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICASS, INSTITUTO DE ECOLOGÍA, A.C., ASSOCIATION POUR LA RECHERCHE ET LE DEVELOPPEMENT DES METHODES ET PROCESSUS INDUSTRIELS (ARMINES)
    Inventors: Primitivo CABALLERO MURILLO, Inés BEPERET ARIVE, Oihane SIMÓN DE GOÑl, Trevor WILLIAMS, Miguel LOPEZ-FERBER
  • PRODUCT AND PROCESS FOR MUCUS VISCOSITY NORMALIZATION
    Publication number: 20160230149
    Abstract: Disclosed are compositions and methods for decreasing the viscosity and/or cohesiveness of and/or increasing the liquefaction of excessively or abnormally viscous or cohesive mucus or sputum. The composition contains a protein or peptide containing a thioredoxin monocysteinic active site in a reduced state and optionally further contains a reducing system.
    Type: Application
    Filed: September 11, 2015
    Publication date: August 11, 2016
    Inventor: Peter B. Heifetz
  • HETERO-TRANSGLYCOSYLASE AND USES THEREOF
    Publication number: 20160230150
    Abstract: The present invention relates to a hetero-transglycosylase protein having cellulose:xyloglucan endotransglucosylase (CXE) activity in addition to mixed-linkage beta-glucan:xyloglucan endotransglucosylase (MXE) activity. The protein may comprise the amino acid sequence of any one of SEQ ID NOs: 2, 6 and 8 or a functional fragment thereof; or an amino acid sequence having at least 60% sequence identity to any one of SEQ ID NO: 2, 6 and 8, or to SEQ ID NO: 2 from amino acid 22 to 280, to SEQ ID NO: 6 from amino acid 26 to 283, or to SEQ ID NO: 8 from amino acid 29 to 287. The invention furthermore relates to an isolated nucleic acid encoding the protein described herein, a chimeric gene comprising, inter alia, the nucleic acid described herein, a vector comprising said chimeric gene, a host cell comprising said vector or said chimeric gene and an according transgenic plant. Further disclosed herein in are a method of producing a transgenic plant and a method of improving properties of cellulosic material.
    Type: Application
    Filed: September 24, 2014
    Publication date: August 11, 2016
    Inventors: Frank Meulewaeter, Ilse Van Den Brande, Stephen C. Fry, Kyle E. Mohier, Lenka Frankova, Tom J. Simmons, Claire Holland, Andrew Hudson
  • MICROORGANISM WITH ENHANCED L-LYSINE PRODUCTIVITY AND METHOD FOR PRODUCING L-LYSINE BY USING SAME
    Publication number: 20160230151
    Abstract: Provided are a beta prime subunit mutant of RNA polymerase, a microorganism of the Corynebacterium genus including a polynucleotide coding the same, and a method for producing L-lysine by culturing the same.
    Type: Application
    Filed: June 24, 2014
    Publication date: August 11, 2016
    Inventors: Sang Hee PARK, Jun Ok MOON, Kwang Ho LEE
  • DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION
    Publication number: 20160230152
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Application
    Filed: April 25, 2016
    Publication date: August 11, 2016
    Inventors: Keith Bauer, Fred Reichert, Thomas W. Myers
  • DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION
    Publication number: 20160230153
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Application
    Filed: April 25, 2016
    Publication date: August 11, 2016
    Inventors: Fred Reichert, Keith Bauer, Thomas W. Myers
  • DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION
    Publication number: 20160230154
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Application
    Filed: April 25, 2016
    Publication date: August 11, 2016
    Inventors: Keith Bauer, Fred Reichert, Thomas W. Myers
  • MODIFIED SULFAMIDASE AND PRODUCTION THEREOF
    Publication number: 20160230155
    Abstract: Disclosed herein are a modified sulfamidase, a composition comprising a modified sulfamidase, as well as methods for preparing a modified sulfamidase and therapeutic use of such a sulfamidase. In particular, the present disclosure relates to a modified sulfamidase comprising substantially no epitopes for glycan recognition receptors, thereby enabling transportation of said sulfamidase across the blood brain barrier of a mammal, wherein said sulfamidase has catalytic activity in the brain of said mammal.
    Type: Application
    Filed: July 22, 2015
    Publication date: August 11, 2016
    Applicant: SWEDISH ORPHAN BIOVITRUM AB (PUBL)
    Inventors: Charlotta BERGHARD, Erik NORDLING, Stefan Svensson GELIUS, Agneta TJERNBERG
  • RECOMBINANT FUSION PROTEIN POSSESSING NUCLEASE AND PHOSPHATASE ACTIVITY
    Publication number: 20160230156
    Abstract: Disclosed herein is a fusion protein possessing both nuclease and phosphatase activities. The described fusion protein simplifies the processing of amplified DNA to degrade residual primers and nucleotide triphosphates and thereby facilitates subsequent DNA analysis.
    Type: Application
    Filed: March 17, 2014
    Publication date: August 11, 2016
    Inventor: Stephen HENDRICKS
  • GLUCOAMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME
    Publication number: 20160230157
    Abstract: The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: April 26, 2016
    Publication date: August 11, 2016
    Applicant: NOVOZYMES A/S
    Inventors: Esben Peter Friis, Leonardo De Maria, Jesper Vind, Thomas A. Poulsen, Allan Svendsen, Steffen Danielsen, Rolf T. Lenhard, Henrik Friis-Madsen, Lars K. Skov
  • Polypeptides Having Cellulolytic Enhancing Activity and Polynucleotides Encoding Same
    Publication number: 20160230158
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: April 26, 2016
    Publication date: August 11, 2016
    Inventors: Yu Zhang, Junxin Duan, Lan Tang, Wenping Wu
  • Multiprotease Therapeutics for Chronic Pain
    Publication number: 20160230159
    Abstract: The invention includes Clostridial neurotoxin derivatives containing at least two light chain endopeptidase domains, and nucleic acids encoding such Clostridial neurotoxin derivatives. In preferred embodiments, the invention includes methods and compositions for the treatment of inflammatory disorders (such as arthritis); chronic pain, such as neuropathic pain and inflammatory pain through the use of such Clostridial neurotoxin derivatives, including those derived from an intact BoNT/A having an LC/E-derived endopeptidase joined to the LC/A endopeptidase.
    Type: Application
    Filed: December 22, 2015
    Publication date: August 11, 2016
    Inventors: James Oliver Dolly, Jiafu Wang, Jianghui Meng
  • MODIFIED BETA-LACTAMASES AND METHODS AND USES RELATED THERETO
    Publication number: 20160230160
    Abstract: The present invention relates to pharmaceuticals and modified beta-lactamases. Specifically, the invention relates to novel recombinant beta-lactamases and pharmaceutical compositions comprising the beta-lactamases. Also, the present invention relates to methods for modifying a beta-lactamase, producing the beta-lactamase and treating or preventing beta-lactam antibiotic induced adverse effects. Furthermore, the present invention relates to the beta-lactamase for use as a medicament and to the use of the beta-lactamase in the manufacture of a medicament for treating or preventing beta-lactam antibiotics induced adverse effects. Still further, the invention relates to a polynucleotide and a host cell comprising the polynucleotide.
    Type: Application
    Filed: April 26, 2016
    Publication date: August 11, 2016
    Inventors: Pertti Koski, Ulla Airaksinen, Katja Valimaki
  • PURIFIED LYSIN PROTEIN
    Publication number: 20160230161
    Abstract: The invention discloses a purified lysin protein for lysing Acinetobacter baumannii cells without pretreatment process using chloroform or EDTA. The purified lysin protein includes a first peptide fragment and a second peptide fragment. The first peptide fragment has an amino acid sequence set forth as SEQ ID NO: 4 and the second peptide fragment has a amino acid sequence set forth as SEQ ID NO: 5.
    Type: Application
    Filed: April 30, 2015
    Publication date: August 11, 2016
    Inventors: Chih-Hsin HUNG, Huei-Ru LUO, Jen-Yi WANG
  • XYLOSE ISOMERASE GENES AND THEIR USE IN FERMENTATION OF PENTOSE SUGARS
    Publication number: 20160230162
    Abstract: The present invention relates to eukaryotic cells which have the ability to isomerise xylose directly into xylulose. The cells have acquired this ability by transformation with nucleotide sequences coding for a xylose isomerase that has one or more specific sequence elements typical for isomerases having the ability of functional expression in yeasts, such as e.g. xylose isomerases obtainable from a bacterium of the genera Clostridium and Fusobacterium or a tunicate form the genus Ciona. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation.
    Type: Application
    Filed: April 7, 2016
    Publication date: August 11, 2016
    Inventors: Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Johannes Adrianus Maria De Bont
  • COMPOSITIONS AND METHODS COMPRISING ASPARTYL-TRNA SYNTHETASES HAVING NON-CANONICAL BIOLOGICAL ACTIVITIES
    Publication number: 20160230163
    Abstract: Isolated aspartyl-tRNA synthetase polypeptides and polynucleotides having non-canonical biological activities are provided, as well as compositions and methods related thereto.
    Type: Application
    Filed: May 4, 2015
    Publication date: August 11, 2016
    Inventors: Ryan A. Adams, Fei Hong, Ji Zhao, Kristi Piehl, Eva R. Armour, Kenny D'Arigo, Leslie A. Greene, Eve Merriman
  • IMPROVED ACETYL-COA CARBOXYLASE VARIANTS
    Publication number: 20160230164
    Abstract: The disclosure relates to acetyl-CoA carboxylase (ACC) variants and host cells expressing them for the production of malonyl-CoA derived compounds including fatty acid derivatives. Further contemplated are methods of producing increased amounts of malonyl-CoA derived compounds and related cell cultures.
    Type: Application
    Filed: September 12, 2014
    Publication date: August 11, 2016
    Applicant: REG Life Sciences, LLC
    Inventors: Derek L. GREENFIELD, Donald E. TRIMBUR, Andreas W. SCHIRMER, Cindy CHANG, Behnaz BEHROUZIAN, Jessica WINGER
  • POLYNUCLEOTIDE ENCODING ACYL-COA SYNTHETASE HOMOLOG AND USE THEREOF
    Publication number: 20160230165
    Abstract: The present invention relates to an acyl-CoA synthetase homolog protein from microorganisms of the genus Mortierella, a polynucleotide encoding the protein, and so on. The invention provides polynucleotides comprising an acyl-CoA synthetase homolog protein gene from, e.g., Mortierella alpina, expression vectors comprising these polynucleotides and transformants thereof, a method for producing lipids or fatty acids using the transformants, food products containing the lipids or fatty acids produced by the method, etc.
    Type: Application
    Filed: February 11, 2016
    Publication date: August 11, 2016
    Applicant: SUNTORY HOLDINGS LIMITED
    Inventor: Misa OCHIAI
  • POLYNUCLEOTIDE ENCODING ACYL-COA SYNTHETASE HOMOLOG AND USE THEREOF
    Publication number: 20160230166
    Abstract: The present invention relates to an acyl-CoA synthetase homolog protein from microorganisms of the genus Mortierella, a polynucleotide encoding the protein, and so on. The invention provides polynucleotides comprising an acyl-CoA synthetase homolog protein gene from, e.g., Mortierella alpina, expression vectors comprising these polynucleotides and transformants thereof, a method for producing lipids or fatty acids using the transformants, food products containing the lipids or fatty acids produced by the method, etc.
    Type: Application
    Filed: February 11, 2016
    Publication date: August 11, 2016
    Applicant: SUNTORY HOLDINGS LIMITED
    Inventor: Misa OCHIAI
  • Method and Apparatus for Reprogramming Living Cells
    Publication number: 20160230167
    Abstract: A method and an apparatus for reprogramming living cells without using viruses. In that method a cocktail comprising at least two transcription factors and a microRNA is transfected into the interior of at least one cell in order to convert this cell into iPS cells or into another type of cell, by storing the cells to be converted in an aqueous environment of the cocktail without viral carriers and focusing a femtosecond laser in a laser scanning microscope with a numerical aperture between 0.9 and 1.5 on a cell membrane of the cell to be reprogrammed and controlling the position of the focus. The exposure period and laser power for the optical treatment of the cell such that the focus depending on the pulse repetition frequency with an output between 7 mW and 100 mW generates a transient small-pore hole with a size up to 500 nm.
    Type: Application
    Filed: February 9, 2016
    Publication date: August 11, 2016
    Inventors: Karsten KOENIG, Aisada UCHUGONOVA
  • APPARATUS FOR SEPARATING PARTICLES UTILIZING ENGINEERED ACOUSTIC CONTRAST CAPTURE PARTICLES
    Publication number: 20160230168
    Abstract: An apparatus for separating particles from a medium includes a capillary defining a flow path therein that is in fluid communication with a medium source. The medium source includes engineered acoustic contrast capture particle having a predetermined acoustic contrast. The apparatus includes a vibration generator that is operable to produce at least one acoustic field within the flow path. The acoustic field produces a force potential minima for positive acoustic contrast particles and a force potential minima for negative acoustic contrast particles in the flow path and drives the engineered acoustic contrast capture particles to either the force potential minima for positive acoustic contrast particles or the force potential minima for negative acoustic contrast particles.
    Type: Application
    Filed: April 14, 2016
    Publication date: August 11, 2016
    Inventors: Gregory Kaduchak, Michael D. Ward
  • RNA INTERFERENCE SUPPRESSION OF NEURODEGENERATIVE DISEASES AND METHODS OF USE THEREOF
    Publication number: 20160230169
    Abstract: The present invention is directed to RNA interference (RNAi) molecules targeted against a nucleic acid sequence that encodes poly-glutamine repeat diseases, and methods of using these RNAi molecules.
    Type: Application
    Filed: September 15, 2015
    Publication date: August 11, 2016
    Applicant: UNIVERSITY OF IOWA RESEARCH FOUNDATION
    Inventors: Beverly L. Davidson, Alejandro Mas Monteys, Ryan Boudreau
  • SIRNA SEQUENCE-INDEPENDENT MODIFICATION FORMATS FOR REDUCING OFF-TARGET PHENOTYPIC EFFECTS IN RNAI, AND STABILIZED FORMS THEREOF
    Publication number: 20160230170
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Application
    Filed: February 4, 2016
    Publication date: August 11, 2016
    Inventors: Nitin PURI, Irudaya CHARLES, Susan MAGDALENO, Alexander VLASSOV, Christopher BURNETT
  • METHODS FOR INDUCING GLUCOSE UPTAKE
    Publication number: 20160230171
    Abstract: The present invention provides a method for inducing glucose uptake in a muscle cell by inhibiting Trim32 protein in the cell and to a method for inducing glucose uptake in a muscle cell, by increasing the abundance of plakoglobin protein in the cell.
    Type: Application
    Filed: September 11, 2014
    Publication date: August 11, 2016
    Inventors: Shenhav COHEN, Goldberg L. ALFRED
  • COMPOSITIONS FOR MODULATING EXPRESSION OF C9ORF72 ANTISENSE TRANSCRIPT
    Publication number: 20160230172
    Abstract: Disclosed herein are compositions and methods for reducing expression of C90RF72 antisense transcript in an animal with C90RF72 antisense transcript specific inhibitors. Such methods are useful to treat, prevent, or ameliorate neurodegenerative diseases in an individual in need thereof. Such C90RF72 antisense transcript specific inhibitors include antisense compounds.
    Type: Application
    Filed: October 14, 2014
    Publication date: August 11, 2016
    Applicant: Ionis Pharmaceuticals, Inc.
    Inventor: Frank Rigo
  • METHODS FOR OVERCOMING GLUCOCORTICOID RESISTANCE AND FOR DETERMINING GLUCOCORTICOID RESISTANCE POTENTIAL IN CANCER
    Publication number: 20160230173
    Abstract: The invention provides methods for overcoming glucocorticoid resistance of cancers by inhibition of CASP1. Also disclosed are diagnostic methods for determining glucocorticoid resistance potential by measuring expression level or promoter methylation status of CASP1 gene and/or NLRP3 gene.
    Type: Application
    Filed: September 16, 2014
    Publication date: August 11, 2016
    Applicant: St. Jude Children's Research Hospital, Inc.
    Inventors: Steven W. Paugh, William E. Evans, Erik Bonten
  • TOLEROGENIC DENDRITIC CELLS TO TREAT INFLAMMATORY BOWEL DISEASE
    Publication number: 20160230174
    Abstract: Methods are disclosed herein for treating or preventing an inflammatory bowel disease in a subject. These methods include administering to a subject an effective amount of tolerogenic dendritic cells, wherein the tolerogenic dendritic cells comprise at least one of an antisense compound specific for CD40, and antisense compound specific for CD80 and an antisense compound specific for CD86.
    Type: Application
    Filed: September 26, 2014
    Publication date: August 11, 2016
    Applicant: University of Pittsburgh - Of the Commonwealth System of Higher Education
    Inventors: Nick Giannoukakis, Massimo M. Trucco
  • METHODS AND COMPOSITIONS FOR RAPID SEAMLESS DNA ASSEMBLY
    Publication number: 20160230175
    Abstract: Provided herein are methods for assembling DNA fragments employing at least three enzymatic activities: DNA polymerase, flap endonuclease, and DNA ligase. Certain aspects include methods for generating closed circular DNA products, e.g., plasmid vectors, by assembling various DNA fragments having complementary ends that hybridize to one another. The resulting circular products can be introduced into host cells and selected for desired properties. Kits for performing the method are also provided.
    Type: Application
    Filed: February 11, 2015
    Publication date: August 11, 2016
    Inventor: Carsten Carstens
  • Recombinant Cells and Organisms Having Persistent Nonstandard Amino Acid Dependence and Methods of Making Them
    Publication number: 20160230176
    Abstract: Novel recombinant cells and recombinant organisms persistently expressing nonstandard amino acids (NSAAs) are provided. Methods of making novel recombinant cells and recombinant organisms dependent on persistently expressing NSAAs for survival are also provided. These methods may be used to make safe recombinant cells and recombinant organisms and/or to provide a selective pressure to maintain one or more reassigned codon functions in recombinant cells and recombinant organisms.
    Type: Application
    Filed: September 26, 2014
    Publication date: August 11, 2016
    Inventors: George M. Church, Daniel J. Mandell, Marc J. Lajoie
  • E. COLI SEPARATOME-BASED PROTEIN EXPRESSION AND PURIFICATION PLATFORM
    Publication number: 20160230177
    Abstract: Provided is a separatome-based peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome of E. coli. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data, and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies facilitating efficient product purification.
    Type: Application
    Filed: September 17, 2014
    Publication date: August 11, 2016
    Inventors: Ellen M. BRUNE, Robert R. BEITLE, Mohammad M. ATAAI, Patrick R. BARTLOW, Ralph L. henry
  • A BACTERIAL SURROGATE FOR TESTING OF ANTIMALARIALS: THYA KNOCKOUT, FOLA KNOCKOUT, FOLP KNOCKOUT, AND FOLK KNOCKOUT BACTERIA FOR TESTING OF INHIBITION OF ANTIFOLATE PATHWAY
    Publication number: 20160230178
    Abstract: In this invention, cell lines are created for enzyme inhibitory testing of inhibitors against Plasmodium falciparum DHFR-TS and HPPK-DHPS. Provided the complementing DHFR-TS and HPPK-DHPS have sufficient activities to support growth of the surrogates in un-supplemented medium, the same surrogates could be used for screening inhibitors of targets against other parasite and pathogen species e.g. Plasmodium vivax, Trypanosoma brucei, Trypanosoma cruzi, Toxoplasma gondii or Mycobacterium tuberculosis. The cell lines in this invention are Escherichia coli strain whose thyA, folA, folK, and folP genes were disrupted using genetic knockout coupled with elimination of antibiotic resistance markers. The thyA KO, folP KO, folK KO, thyAfolA KO, folKfolP KO, thyAfolAfolP KO, thyAfolAfolK KO and thyAfolAfolKfolP KO E.
    Type: Application
    Filed: September 18, 2014
    Publication date: August 11, 2016
    Applicant: National Science And Technology Development Agency
    Inventors: Nattida SUWANAKITTI, Yuwadee TALAWANICH, Yongyuth YUTHAVONG, Sumalee KAMCHONWONGPAISAN
  • SHUTTLE PLASMID REPLICABLE IN BOTH CLOSTRIDIA AND ESCHERICHIA COLI
    Publication number: 20160230179
    Abstract: Disclosed is a shuttle plasmid replicable in both Clostridium and Escherichia coli comprising: a base sequence of a first replication origin replicable in Escherichia coli; and a base sequence of a second replication origin derived from pUB 110 plasmid.
    Type: Application
    Filed: February 4, 2016
    Publication date: August 11, 2016
    Inventor: Sang-Hyun LEE
  • ENGINEERING THE PLANT ACTIN CYTOSKELETON TO MODULATE CELL WALL PROPERTIES
    Publication number: 20160230180
    Abstract: A method for controlling the mechanical properties of the cell wall and polarized growth in a cell that includes selectively manipulating the cell cytoskeleton is presented herein.
    Type: Application
    Filed: February 3, 2016
    Publication date: August 11, 2016
    Applicant: PURDUE RESEARCH FOUNDATION
    Inventor: Daniel B Szymanski
  • TOBACCO NICOTINE DEMETHYLASE GENOMIC CLONE AND USES THEREOF
    Publication number: 20160230181
    Abstract: The present invention features tobacco nicotine demethylase nucleic acid and amino acid sequences, tobacco plants and plant components containing such sequences, including tobacco plants and plant components having reduced expression or altered enzymatic activity of nicotine demethylase, methods of use of nicotine demethylase sequences to create plants having altered levels of nornicotine or N?-nitrosonornicotine (“NNN”) or both relative to a control plant, as well as tobacco articles having reduced levels of nornicotine or NNN.
    Type: Application
    Filed: April 22, 2016
    Publication date: August 11, 2016
    Inventor: Dongmei Xu
  • POLYPEPTIDE REGULATING AND CONTROLLING THE FORMATION OF PLANT AGRONOMIC TRAIT OR YIELD TRAIT, AND USE OF POLYPEPTIDE
    Publication number: 20160230182
    Abstract: The invention relates to a polypeptide for regulating agronomic characters and yield characters of plants and application thereof. The invention alters agronomic characters and yield characters of plants such as root development, tillering ability, heading period, panicle structure, grains number per panicle, grain morphology, and 1000 grains weight by directional regulating expression level of cytokinin-O-glycosyltransferase 1 in plants, thus achieve the aim of modifying plant type, panicle structure, grain morphology and weight, increasing yield.
    Type: Application
    Filed: June 24, 2014
    Publication date: August 11, 2016
    Applicant: SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCES
    Inventors: Fangqing GUO, Xiaoling SHANG, Hua TIAN
  • TRANSGENIC PLANTS WITH ENHANCED AGRONOMIC TRAITS
    Publication number: 20160230183
    Abstract: This invention provides recombinant DNA constructs, transgenic plant nuclei and cells with such recombinant DNA construct for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
    Type: Application
    Filed: February 8, 2016
    Publication date: August 11, 2016
    Inventors: Mark Scott Abad, Jeffery E. Ahrens, Alice Clara Augustine, Erin Bell, Robert J. Bensen, Srikanth Venkatachalayya, Paolo Castiglioni, R. Eric Cerny, Jaishree M. Chittoor-Vijayanath, Maureen Daley, Jill Deikman, Molian Deng, Todd DeZwaan, Stephen Duff, Michael D. Edgerton, Bradon J. Fabbri, Jason Fenner, Karen Gabbert, Meghan Galligan Donnarummo, Garrett J. Lee, Barry S. Goldman, Deborah J. Hawkins, Steve He, Xianfeng Chen, Balasulojini Karunanandaa, Paul Loida, Savitha Madappa, Robert J. Meister, Ming Peng, Donald E. Nelson, Anand Pandravada, Obed Patty, Marie Petracek, Rajani Monnanda Somaiah, Monica P. Ravanello, Richard Johnson, Thomas G. Ruff, Rick A. Sanders, Beth Savidge, Steven H. Schwartz, Shobha Char, Padmini Sudarshana, Jindong Sun, Rebecca L. Thompson-Mize, Virginia Ursin, Dale V. Val, Tymagondlu V. Venkatesh, Kammaradi Vidya, K. Vijayalakshmi, Todd C. Weber, Jingrui Wu, Wei Zheng, Zhidong Xie, Dhanalakshmi Ramachandra
  • HERBICIDE-RESISTANT SUNFLOWER PLANTS, POLYNUCLEOTIDES ENCODING HERBICIDE-RESISTANT ACETOHYDROXYACID SYNTHASE LARGE SUBUNIT PROTEINS, AND METHODS OF USE
    Publication number: 20160230184
    Abstract: Herbicide-resistant sunflower plants, isolated polynucleotides that encode herbicide-resistant and wild-type acetohydroxyacid synthase large subunit (AHASL) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.
    Type: Application
    Filed: February 26, 2016
    Publication date: August 11, 2016
    Applicants: BASF Aktiengesellschaft, Nidera S.A.
    Inventors: Carlos Alberto SALA, Mariano Bulos, Sherry R. Whitt, Robert Ascenzi, Adriana Mariel Echarte
  • COMPOSITIONS AND METHODS FOR CONTROLLING DIABROTICA
    Publication number: 20160230185
    Abstract: The present invention provides methods for controlling invertebrate pest infestations, for instance in plants, and related compositions and polynucleotides useful in such methods. More specifically, the present invention provides polynucleotides and methods of use thereof for modifying the expression of genes in an invertebrate pest, for instance through RNA interference.
    Type: Application
    Filed: March 12, 2014
    Publication date: August 11, 2016
    Applicant: Monsanto Technology LLC
    Inventors: James A. Baum, Renata Bolognesi, Lex Evan Flagel, Gerrit Cornelis Segers
  • COMPOSITIONS AND METHODS FOR CONTROLLING DIABROTICA
    Publication number: 20160230186
    Abstract: The present invention provides methods for controlling invertebrate pest infestations, for instance in plants, and related compositions and polynucleotides useful in such methods. More specifically, the present invention provides polynucleotides and methods of use thereof for modifying the expression of genes in an invertevrate pest, for instance through RNA interference.
    Type: Application
    Filed: March 12, 2014
    Publication date: August 11, 2016
    Applicant: Monsanto Technology LLC
    Inventors: James A. Baum, Renata Bolognesi, Gerrit Cornelis Segers
  • COMPOSITIONS AND METHODS FOR IMPROVING INSECT RESISTANCE
    Publication number: 20160230187
    Abstract: Compositions and methods for improving the pest resistance of plants. Plants and plant parts identified, selected and/or produced using the compositions and the methods.
    Type: Application
    Filed: September 17, 2014
    Publication date: August 11, 2016
    Inventors: Aiping Zheng, Ping Li, Qiao Li, Jun Zhu, Qiming Deng, Shiquan Wang, Shuangcheng Li
  • METHOD OF DE-DIFFERENTIATING AND RE-DIFFERENTIATING SOMATIC CELLS USING RNA
    Publication number: 20160230188
    Abstract: RNA prepared by in vitro transcription using a polymerase chain reaction (PCR)-generated template can be introduced into a cell to modulate cell activity. This method is useful in de-differentiating somatic cells to pluripotent, multipotent, or unipotent cells; re-differentiating stem cells into differentiated cells; or reprogramming of somatic cells to modulate cell activities such as metabolism. Cells can also be transfected with inhibitory RNAs, such as small interfering RNA (siRNA) or micro RNA (miRNA), or combinations thereof to induce reprogramming of somatic cells. For example, target cells are isolated from a donor, contacted with one or more RNA's causing the cells to be de-differentiated, re-differentiated, or reprogrammed in vitro, and administered to a patient in need thereof. The resulting cells are useful for treating one or more symptoms of a variety of diseases and disorders, for organ regeneration, and for restoration of the immune system.
    Type: Application
    Filed: February 1, 2016
    Publication date: August 11, 2016
    Inventors: Peter M. Rabinovich, Sherman M. Weissman
  • NANOPARTICLE-MEDIATED GENE DELIVERY, GENOMIC EDITING AND LIGAND-TARGETED MODIFICATION IN VARIOUS CELL POPULATIONS
    Publication number: 20160230189
    Abstract: An improved nanoparticle for transfecting cells is provided. The nanoparticle includes a core polyplex and a silica coating on the core polyplex and, optionally, a polymer attached to an outer surface of the silica coating , where the polyplex includes an anionic polymer, a cationic polymer, a cationic polypeptide, and a polynucleotide. Also provided is an improved method of modifying intracellular polynucleotides. The method includes contacting a cell with a nanoparticle that includes a core polyplex and a silica coating on the core polyplex and, optionally, a polymer attached to an outer surface of the silica coating, where the polyplex includes an anionic polymer, a cationic polymer, a cationic polypeptide, and a polynucleotide.
    Type: Application
    Filed: September 23, 2014
    Publication date: August 11, 2016
    Applicant: RENSSELAER POLYTECHNIC INSTITUTE
    Inventors: Shiva Prasad KOTHA, Andre Ronald WATSON, Vaibhav A. PANDIT
  • Lentiviral Vectors Having a Mutated Integrase Protein and uses Thereof
    Publication number: 20160230190
    Abstract: The present invention relates to a lentiviral vector wherein the expressed integrase protein comprises at least one point mutation consisting of the substitution of the aspartic acid residue at position 167 by an amino acid selected from the group consisting of histidine, arginine and lysine.
    Type: Application
    Filed: September 17, 2014
    Publication date: August 11, 2016
    Inventors: Che Serguera, Francois Lachapelle, Noelle Dufour, Muhammad Qamar Saeed
  • RETROVIRAL VECTORS
    Publication number: 20160230191
    Abstract: There is disclosed a retroviral vector comprising a primer binding site, a long terminal repeat and an RNA packaging sequence, wherein the RNA packaging sequence is located 3? of the long terminal repeat and no long terminal repeat is located 3? of the RNA packaging sequence such that reverse transcription initiated at the primer binding site does not lead to reverse transcription of the RNA packaging sequence into vector DNA in a target cell. Also described is a host cell, a virion, a pharmaceutical composition, a method and uses including or involving the vector described above. Further, a cell or transgenic animal produced by using the vector is also described.
    Type: Application
    Filed: October 16, 2014
    Publication date: August 11, 2016
    Inventors: Conrad Vink, Steven Howe
  • TRITERPENOIDS HIGH YIELDING STRAIN OF ANTRODIA CINNAMOMEA AND USE THEREOF
    Publication number: 20160230192
    Abstract: The present invention discloses a triterpenoids high yielding strain of Antrodia cinnamomea and use thereof. The accession number of the Antrodia cinnamomea strain with a high triterpenoids yield is CCTCC M2013359. As for the strain mycelium, the growth rate is fast, the triterpenoids output is high, and the genetic properties are stable, so the strain mycelium is suitable for industrialized fermentation. Compared with the original strain, the mycelium output under a liquid culturing condition is increased by more than 1.5 times, and the intracellular triterpenoids output is increased by 2 to 3 times, the fermentation period is shortened to 65 hours compared with the original more than 10 days; and the total triterpenoids output under a solid culturing condition is increased by 1 to 2 times, and the fermentation period is shortened to 10 days compared with the original more than 90 days.
    Type: Application
    Filed: August 20, 2013
    Publication date: August 11, 2016
    Inventors: Zongjie Zhao, Haitao Xie, Qingsong Cai
  • SOLID WASTE TREATMENT WITH CONVERSION TO GAS AND ANAEROBIC DIGESTION
    Publication number: 20160230193
    Abstract: Waste solids are treated by pyrolysis at a temperature over 700 degrees C. to produce char and a gas. The gas is treated in an anaerobic digester. In one system, gas and digestate are brought into contact in a diffusion cone. In another option, headspace gas above the digestate is re-circulated through the digestate, for example by way of an eductor downstream of the diffusion cone.
    Type: Application
    Filed: February 4, 2016
    Publication date: August 11, 2016
    Inventors: Juan Carlos JOSSE, Yaniv D. SCHERSON