Patents Issued in March 2, 2017
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Publication number: 20170059527Abstract: In a method of eddy current testing via an eddy current testing instrument, in response to the eddy current test instrument experiencing acceleration, the eddy current test instrument outputs an alternating magnetic field which induces eddy currents in a specimen and detects the induced eddy currents. When the eddy current test instrument is not experiencing acceleration, after a delay, the eddy current test instrument withholds outputting the alternating magnetic field to, thereby, conserve power.Type: ApplicationFiled: August 30, 2016Publication date: March 2, 2017Inventor: Edward Lopez
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Publication number: 20170059528Abstract: Provided is to ultrasonic probe used in scanning defect of welded zone of tube, the ultrasonic probe includes a probe body having a predetermined length, a connection bar connected to one end of the probe body, a phased array ultrasonic sensor installed on one side surface of the probe body to scan positions and shapes of the defects, and a pair of time of flight diffraction (TOFD) sensors respectively installed in the same line with the phased array ultrasonic sensor therebetween to scan depths of the defects. The present invention having the above-described configurations may significantly reduce a time taken to scan and accurately scan the positions, the shapes, and the depths of the defects.Type: ApplicationFiled: July 15, 2016Publication date: March 2, 2017Inventors: Un Hak SEONG, Sung Woo RYU, Won Il KIM, Su Jin BAE, Byeong Lae CHO, Jong Bum WON
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Publication number: 20170059529Abstract: An additive manufacturing process, including: selectively-heating a layer of powder (18) to form a solid deposit layer (10) having a solid deposit (28), where the solid deposit layer constitutes part (24) of a component, via a selective laser heating process; propagating ultrasonic energy waves (50, 60) through the solid deposit prior to completion of the component by using a wave generating laser (40) set apart from a surface (44) of the solid deposit to direct a wave-generating laser beam (42) at the surface; detecting propagated ultrasonic energy waves (62); assessing the propagated ultrasonic waves for information about a physical characteristic of the solid deposit; and forming another solid deposit layer (80) in response to the information obtained about the solid deposit.Type: ApplicationFiled: August 24, 2015Publication date: March 2, 2017Inventors: Ahmed Kamel, Anand A. Kulkarni
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Publication number: 20170059530Abstract: An acoustic wave probe includes a support member including a plurality of through holes and a concave portion which is concave facing an object disposed in a measurement position at the time of measurement, and an acoustic wave transducer unit including at least a transducer. The acoustic wave transducer unit is mounted in the through hole facing approximately a center of curvature of the concave portion, and a thickness of the acoustic wave transducer unit is thinner on a side of the center of curvature.Type: ApplicationFiled: August 23, 2016Publication date: March 2, 2017Inventor: Atsushi Kandori
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Publication number: 20170059531Abstract: Apparatus and methods for ultrasonic inspection of elongated composite members in a single scan pass using pulse echo phased arrays operating in a bubbler method. The system concept is fully automated by integrating an inspection probe assembly to a robot and using the robot to move the inspection probe assembly along the part (i.e., outside of an inspection tank); and by integrating tooling fixtures that move out of the way as the inspection probe assembly travels along the length of the part during the inspection. In addition, the system allows for generally elongated composite members having lengthwise variation in shape, curvature and dimensions.Type: ApplicationFiled: August 26, 2015Publication date: March 2, 2017Applicant: THE BOEING COMPANYInventors: Barry A. Fetzer, Justin D. Serrill
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Publication number: 20170059532Abstract: A system for detecting rodent activity in an area. A series of detectors that calibrated to detect rodent vocalizations are placed in a geographic area. One or more of the detectors detect rodent vocalizations and transmits data notifying a central computer of detected vocalizations. The computer is provided with a program that generates a report of incidences and locations of detected vocalizations.Type: ApplicationFiled: August 26, 2016Publication date: March 2, 2017Inventors: Jeffrey Frisch, Jonathan Frisch
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Publication number: 20170059533Abstract: A cooling-assisted inside needle capillary adsorption trap device for sampling and delivering volatile and semi-volatile analytes to an analytical device is disclosed. The device includes an inside needle capillary adsorption trap having a first end and a second end and a side aperture located between the first and second ends. The side aperture enables entering the carrier gas into the INCAT and flowing upon the surface of the sorbent (when injected into the GC injector) for complete releasing and eluting of the analytes from the interior surface of the needle. A sorbent is multiwall carbon nanotube/polyaniline and is coated onto the interior surface of the needle between the second end and the side aperture to entrap an analyte within a sample. The cooling-assisted inside needle capillary adsorption trap device also includes a cooling device configured to cool the sorbent.Type: ApplicationFiled: September 26, 2016Publication date: March 2, 2017Inventors: Alireza Ghiasvand, Fatemeh Yazdankhah, Farzaneh Zarghami
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Publication number: 20170059534Abstract: A system reduces unwanted noise due to pump movement. The system includes a pump including a non-conductive piston extending into a pumping chamber for pumping the sample fluid along an analysis fluid path, a wash chamber, and a seal fluidly separating the pumping chamber from the wash chamber. The system also includes a wash path supplying a wash fluid from a wash reservoir to the wash chamber, an electrical conductor electrically connecting the analysis fluid path to the wash fluid path, and a ground conductor electrically connecting the electrical conductor to ground via a resistor. The electrical conductors and resistor combination between the chambers reduces unwanted noise in the conductivity detector due voltage potentials between the eluent and wash chambers created as the piston moves between the chambers.Type: ApplicationFiled: August 28, 2015Publication date: March 2, 2017Inventors: Michael J. McADAMS, Ihab ELZIND
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Publication number: 20170059535Abstract: The present invention relates to newborn screening kits, methods, stable isotopically-labeled internal standards or internal standard solution for high throughput screening and analysis of metabolic disorders using liquid chromatography mass spectrometry (LC-MS) are provided. The metabolic disorders can be amino acid, organic acid or fatty acid oxidation disorders, and particularly urea cycle disorders or deficiencies, hyperammonemia, Hyperornithinemia-hyperammonemia-homocitrullinuria (HHH), and/or argininosuccinic aciduria. The newborn screening kits, methods, stable isotopically-labeled internal standards or internal standard solution are particularly useful for newborn screening (NBS) of metabolic disorders.Type: ApplicationFiled: September 2, 2016Publication date: March 2, 2017Inventors: Géraldine CARRARD, Ralph FINGERHUT
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Publication number: 20170059536Abstract: A cannabinoid analysis system includes a solvent input port configured to receive a flow of a solvent, a sample input port configured to receive a chemical mixture including a sample, a pump configured to move the solvent and chemical mixture, an extraction cartridge coupled to the pump and configured to separate a combination of the chemical mixture and the flow of the solvent into two or more partially-separated chemical components, a spectroscopy cell configured to generate a chromatogram including two or more overlapping absorption peaks corresponding to the two or more partially-separated chemical components, and a controller configured to determine a chemical concentration of each partially-separated chemical component of the two or more partially-separated chemical components.Type: ApplicationFiled: August 31, 2016Publication date: March 2, 2017Inventor: Dylan Elmer Wilks
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Publication number: 20170059537Abstract: The present invention relates to a method for analyzing mixture components, comprising: (1) separating a mixture sample by using chromatographic technology, to obtain a preliminary chromatogram; (2) sampling as required any one interval in the preliminary chromatogram obtained in step (1), to obtain a series of spectrograms in different retention times, which are referred to as compound spectrograms; (3) performing calculation, by using a series of methods of an entropy minimum algorithm, in the mixture spectrograms obtained in step (2), to obtain each reconstructed pure spectrum and a corresponding pseudo concentration thereof. The method combines the chromatographic technology and the entropy minimum algorithm, and overcomes dependence on component separation in an existing analysis method, making separation not important any more. There is no need to completely separate each component in the mixture. Meanwhile, a prerequisite for using the entropy minimum algorithm is met.Type: ApplicationFiled: February 12, 2015Publication date: March 2, 2017Inventor: Huajun Zhang
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Publication number: 20170059538Abstract: Disclosed are a chemochromic nanoparticle, a method for manufacturing the chemochromic nanoparticle, and a hydrogen sensor comprising the chemochromic nanoparticle. In particular, the chemochromic nanoparticle has a core-shell structure such that the chemochromic nanoparticle and comprises a core comprising a hydrated or non-hydrated transition metal oxide; and a shell comprising a transition metal catalyst.Type: ApplicationFiled: April 26, 2016Publication date: March 2, 2017Inventors: Yong Gyu Noh, Hyun Joon Lee, Hyo Sub Shim, Hyung Tak Seo, Yeong An Lee, Shankara S. Kalanur
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Publication number: 20170059539Abstract: A metering system that may find use to generate values for measured parameters of materials. The metering system may be configured with a metrology device configured to generate a first signal in digital format to convey information about material in a conduit. The metering system may also include an accessory coupled with the metrology device, the accessory configured to use the information of the first signal to generate a second signal, the second signal conveying information that defines a measured parameter for the material. In one implementation, the accessory comprises executable instructions that configure the accessory to exchange information with the metrology device so as to verify a regulatory status for the metrology device.Type: ApplicationFiled: November 20, 2016Publication date: March 2, 2017Inventor: Roman Leon Artiuch
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Publication number: 20170059540Abstract: Provided is a gas sensor in which a protective cover has no looseness even by repetitive use. The gas sensor includes a sensor element for detecting a predetermined measurement target gas component in a measurement gas; a protective cover which protects a side of one end portion of the sensor element; and a housing which houses the sensor element. The protective cover includes a brim portion at one end portion of an outer surface of the protective cover. The brim portion is brought into contact with a contact surface provided in said housing, and is held between a swaging portion and the contact surface, the swaging portion extending from the contact surface while bending, so that the protective cover is fixed to the housing. The brim portion is tilted at a tilt angle of 5° or more and 15° or less relative to a plane vertical to a central axis of the gas sensor.Type: ApplicationFiled: August 19, 2016Publication date: March 2, 2017Inventors: Yusuke WATANABE, Takao MURASE, Masashi YASUI
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Publication number: 20170059541Abstract: An apparatus is described to detect, measure or both, key compounds, such as sulfur dioxide (SO2) associated withoutgassed elemental compounds suggestive of the composition of one or more constituents in a liquid.Type: ApplicationFiled: September 1, 2016Publication date: March 2, 2017Applicant: TrenLot, Inc.Inventor: Gregory Howard
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Publication number: 20170059542Abstract: A system and method for a beverage container configured to test for a contaminated beverage in the beverage container comprises a base of the beverage container, an upper portion of the beverage container, and a sidewall with an inner surface and an outer surface that extends from the base to the upper portion, and a testing material. A portion of the beverage container is the testing material configured to visibly react when the portion of the beverage container is contacted with the contaminated beverage.Type: ApplicationFiled: November 15, 2016Publication date: March 2, 2017Inventors: Michael T. Abramson, John C. MacDonald
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Publication number: 20170059543Abstract: A water sensing assembly includes: a valve box securely mountable on an underground water pipe; a sensor mounted inside the valve box, the sensor configured to sense a property of water within the underground water pipe; a top section connected to the valve box; and an electrical communication device mounted at a top portion of the adjustable top section such that the electrical communication device is positioned at or near the surface of the ground.Type: ApplicationFiled: November 10, 2016Publication date: March 2, 2017Inventors: Kenneth A. Clark, Timofey Sitnikov, Paul Gifford
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Publication number: 20170059544Abstract: Provided are apparatus and systems using mine spectroscopic data at various stages of the hydrocarbon extraction process. The spectrometers may be mounted on various equipment components at the various stages of the hydrocarbon extraction process to passively collect energy reflected from objects. The obtained data may be used to determine mineralogy, bitumen saturation, bitumen viscosity, and grain size distribution in the mining operations.Type: ApplicationFiled: August 5, 2016Publication date: March 2, 2017Inventor: Sherry L. STAFFORD
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Publication number: 20170059545Abstract: A rotating device includes a rotating shaft, a rotating disc, a left clamping disc, a right clamping disc, an upper clamping disc, and a recovering component. The upper clamping disc, the left clamping disc, and the right clamping disc are sleeved on the rotating shaft. The upper clamping disc fixes the rotating disc, the left clamping disc, and the right clamping disc on the rotating shaft. The recovering component abuts between a side of the left clamping disc and a side of the right clamping disc. A clamping zone is formed between the other side of the left clamping disc and the other side of the right clamping disc for clamping the detecting disc, and an overlapping zone is formed between the side of the left clamping disc and the side of the right clamping disc and opposite to the clamping zone.Type: ApplicationFiled: August 11, 2016Publication date: March 2, 2017Inventors: Chen-Fu Chang, Chien-Hui Hsu, Sheng-Wen Chiu, Chuan-Tsung Feng
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Publication number: 20170059546Abstract: A nanopore cell includes a conductive layer and a working electrode disposed above the conductive layer and at the bottom of a well into which an electrolyte may be contained, such that at least a portion of a top base surface area of the working electrode is exposed to the electrolyte. The nanopore cell further includes a first insulating wall disposed above the working electrode and surrounding a lower section of a well, and a second insulating wall disposed above the first insulating wall and surrounding an upper section of the well, forming an overhang above the lower section of the well. The upper section of the well includes an opening that a membrane may span across, and wherein a base surface area of the opening is smaller than the at least a portion of the top base surface area of the working electrode that is exposed to the electrolyte.Type: ApplicationFiled: August 31, 2015Publication date: March 2, 2017Inventors: John Foster, Morgan Mager
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Publication number: 20170059547Abstract: Molecular sensing system including: a sensing device (5) comprising at least one support layer (10), and an active layer (6) mounted on said support layer and having at least one nano-pore (12) configured for translocation of a molecular analyte (18) therethrough; an electrically conducting liquid (4) in contact with the active layer in a region around said nano-pore; and a signal processing circuit (7) comprising an ionic current circuit (8) configured to generate and measure an ionic current (Ii) in the electrically conducting liquid influenced by the translocation of the molecular analyte through the nano-pore. The molecular sensing device of the invention allows for single-nucleotide discrimination and detection of the specific sequence within ssDNA.Type: ApplicationFiled: February 13, 2015Publication date: March 2, 2017Inventors: JIANDONG FENG, KE LIU, ALEKSANDRA RADENOVIC
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Publication number: 20170059548Abstract: A nanodevice includes a reservoir filled with conductive fluid and a membrane separating the reservoir. A nanopore is formed through the membrane having electrode layers separated by insulating layers. A certain electrode layer has a first type of organic coating and a pair of electrode layers has a second type. The first type of organic coating forms a motion control transient bond to a molecule in the nanopore for motion control, and the second type forms first and second transient bonds to different bonding sites of a base of the molecule. When a voltage is applied to the pair of electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels via the first and second transient bonds formed to be measured as a current signature for distinguishing the base. The motion control transient bond is stronger than first and second transient bonds.Type: ApplicationFiled: November 11, 2016Publication date: March 2, 2017Inventors: Ali Afzali-Ardakani, Stefan Harrer, Binquan Luan, Hongbo Peng, Stephen M. Rossnagel, Ajay K. Royyuru, Gustavo A. Stolovitzky, Philip S. Waggoner
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METHODS AND SYSTEMS FOR MEASURING AND USING THE OXIDATION-REDUCTION POTENTIAL OF A BIOLOGICAL SAMPLE
Publication number: 20170059549Abstract: Methods and systems for measuring and using the oxidation-reduction characteristics of a biological sample are provided. The system generally includes a test strip and a readout device. A fluid sample is placed in the test strip, and the test strip is in turn operatively connected to the readout device. The readout device provides a controlled current that is sent across the fluid in the sample chamber. In addition, the readout device identifies an inflection point or transition time at which the voltage between contacts of the test strip is changing at the highest rate. The oxidation-reduction capacity of the sample is taken as the integral of the current profile from the time at which current begins to be supplied to the sample to the identified transition time.Type: ApplicationFiled: May 5, 2016Publication date: March 2, 2017Inventors: Raphael Bar-Or, David Bar-Or, Leonard T. Rael -
Publication number: 20170059550Abstract: The present disclosure provides a biological fluid collection device, such as a blood collection device, that is adapted to receive a multi-component blood sample having a cellular portion and a plasma portion and quickly, efficiently and cost-effectively separate the plasma portion from the sample. A pressure gradient can be applied across a separation member to facilitate movement of the plasma portion ahead of the cellular portion. The present disclosure allows for both passive and active plasma separation and dispensing.Type: ApplicationFiled: August 31, 2016Publication date: March 2, 2017Inventors: Kishore K. Bokka Srinivasa Rao, Daniel J. Marchiarullo, Milan Ivosevic
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Publication number: 20170059551Abstract: A system for preserving a blood sample and removing hematocrit includes a casing, the casing having a sample port and a lateral flow strip in the casing, the lateral flow strip receiving a sample through the sample port, and flowing the sample down a length of the lateral flow strip.Type: ApplicationFiled: August 31, 2016Publication date: March 2, 2017Applicant: Polymer Technology Systems, Inc.Inventors: Aniruddha Patwardhan, Gary L. Hughes
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Publication number: 20170059552Abstract: This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a displacement fluid, a collector, and a primary vessel. In another implementation, the system includes a processing vessel, a displacement fluid, a collector, and a primary vessel.Type: ApplicationFiled: November 14, 2016Publication date: March 2, 2017Applicant: RareCyte, Inc.Inventors: Daniel Campton, Joshua Nordberg, Steve Quarre, David Stewart, Ronald Seubert
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Publication number: 20170059553Abstract: To provide a biomarker which is a metabolite included in a biological sample, and which can be collected in a noninvasive method and detected in a convenient analysis method, and which varies according to progression of pathological conditions of irritable bowel syndrome (IBS). Furthermore, such biomarkers are useful for determination of the presence or absence of morbidity of IBS, determination of severity of IBS, determination of types of IBS, determination of necessity of treatment of IBS, and confirmation of drug efficacy of an IBS therapeutic agent.Type: ApplicationFiled: February 18, 2015Publication date: March 2, 2017Applicant: ONO PHARMACEUTICAL CO., LTD.Inventors: Shin Fukudo, Seishi Katsumata, Hideaki Tada, Akio Hayashi, Chie Murata
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Publication number: 20170059554Abstract: Disclosed is a method for monitoring use of a tobacco product by a subject, the method comprising providing the tobacco product to the subject and, at one or more times after providing the tobacco product, collecting a sample of exhaled breath from the subject and subjecting the collected sample to analysis to determine amount and/or concentration of a marker, wherein the amount and/or concentration of the marker is related to use of the tobacco product by the subject.Type: ApplicationFiled: September 2, 2015Publication date: March 2, 2017Inventor: Gary Dull
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Publication number: 20170059555Abstract: Disclosed herein is a platform for holding a biological sample with a movable radiation source that can be used to expose the sample to controlled dosages of radiation in a targeted manner. In some embodiments, the platform includes a support structure and a cartridge for holding a sample. In some embodiments, the support structure includes a support for holding the sample, which may be in a well in the support structure. A radiation source can be adjusted relative to the sample (for example, the distance between the sample and the radiation source is controlled) in order to administer a particular dose of radiation to the sample.Type: ApplicationFiled: August 30, 2016Publication date: March 2, 2017Applicant: Los Alamos National Security, LLCInventors: Rashi Iyer, Piyush Bajaj, Jen-Huang Huang
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Publication number: 20170059556Abstract: The present invention is a composition identified as a region of ralA binding protein 1, wherein the region neighbors a membrane-associated portion of the ralA binding protein 1, reduces transport activity and membrane association of the ralA binding protein 1 and kills cells undergoing uncontrolled cell growth in a subject that has cells undergoing uncontrolled cell growth. The region is used to generate medicines that kill malignant cells and tumorigenic cells. Medicines may be in the form of antibodies, si-RNA and small molecules that recognize the region.Type: ApplicationFiled: November 10, 2016Publication date: March 2, 2017Applicant: Board of Regents, The University of Texas SystemInventors: Sanjay Aswasthi, Sharad S. Singhal, Sushma Yadav
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Publication number: 20170059557Abstract: A method and device are disclosed for inducing mechanical stress in a cellular sample to evaluate mechanotransduction in the cellular sample. In one embodiment, the mechanical stress is induced by generating a microcavitation bubble in the cellular sample using a pulsed energy. The microcavitation bubble creates a microtsunami, which provides a transient, impulsive mechanical stress on the cellular sample, forming a gradient of effects at distances away from the microcavitation bubble.Type: ApplicationFiled: August 31, 2016Publication date: March 2, 2017Inventors: Elliot L. Botvinick, Vasan Venugopalan, Jonathan Compton, Amy Hellman
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Publication number: 20170059558Abstract: The present invention pertains to a method for standardizing the sensitivity of induced pluripotent stem cell (iPS)-derived neurons to a neurotoxin polypeptide, comprising the steps of: a) cultivating different batches of induced pluripotent stem cell-derived neurons in a cell culture medium comprising GT1b for at least 3 hours; b) contacting the different batches of induced pluripotent stem cell-derived neurons of step a) with a neurotoxin polypeptide; c) cultivating the different batches of induced pluripotent stem cell-derived neurons of step b) for at least 24 hours in the presence of GT1b under conditions which allow for the neurotoxin polypeptide to exert its biological activity, thereby standardizing the sensitivity of the induced pluripotent stem cell-derived neurons to a neurotoxin polypeptide.Type: ApplicationFiled: February 18, 2015Publication date: March 2, 2017Inventors: Karl-Heinz EISELE, Gerd MANDER
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Publication number: 20170059559Abstract: C1q is shown to be expressed in neurons, where it acts as a signal for synapse elimination. Methods are provided for protecting or treating an individual suffering from adverse effects of synapse loss. These findings have broad implications for a variety of clinical conditions, including Alzheimer's disease.Type: ApplicationFiled: November 11, 2016Publication date: March 2, 2017Applicant: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Ben A. Barres, Beth A. Stevens
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Publication number: 20170059560Abstract: A urine sample analyzer is disclosed. A measurement specimen containing a urine sample flows through flow cell, and light is applied to the measurement specimen flowing through flow cell. Then, a signal corresponding to light received from particles contained in the urine sample is acquired. Thereafter, presence of sperms in the urine sample is analyzed based on parameters reflecting the waveform of the acquired signal.Type: ApplicationFiled: August 19, 2016Publication date: March 2, 2017Applicant: SYSMEX CORPORATIONInventors: Mitsumasa SAKAMOTO, Shota TATEYAMA, Masanori KAWANO
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Publication number: 20170059561Abstract: The subject invention provides the materials and methods for fabricating and using an electrochemical immunosensing device to detect a target antigen. In a preferred embodiment, the subject invention utilizes Camelid-derived, single-domain antibodies as the sensing agents immobilized onto the surface of the immunosensing device, more specifically, the working electrode of the immunosensing device. Furthermore, embodiments of the subject invention provide means for increasing the device's detection sensitivity by utilizing a working electrode optionally configured with an array of interdigitated electrodes.Type: ApplicationFiled: August 25, 2016Publication date: March 2, 2017Applicant: The Florida International University Board of TrusteesInventors: Shekhar BHANSALI, Aparajita SINGH, Syed Khalid PASHA
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Publication number: 20170059562Abstract: A processing mechanism unit forms an immune complex containing the test substance in the sample and a labeled substance contained in a labeled reagent on a magnetic particle contained in a solid-phase reagent. The unit liberates the immune complex from the magnetic particle with a liberating reagent. A transfer unit includes a first holder to hold a first reaction chamber, and transfers the first reaction chamber received in the first holder at a first position to a second position while magnetically collecting the magnetic particle in the first reaction chamber by a magnetic source. A dispenser aspirates a liquid phase containing the liberated immune complex in the first reaction chamber transferred to the second position, and dispenses the liquid phase into a second reaction chamber. A detector detects a signal based on a label contained in the immune complex in the liquid phase dispensed into the second reaction chamber.Type: ApplicationFiled: August 19, 2016Publication date: March 2, 2017Applicant: SYSMEX CORPORATIONInventors: Yutaka KAWAMOTO, Toshikatsu FUKUJU
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Publication number: 20170059563Abstract: A fully integrated miniaturized optical biosensor and methods of making the same are disclosed. The biosensor may include a fluid transport system and an optical system.Type: ApplicationFiled: May 1, 2015Publication date: March 2, 2017Inventors: Joseph Smith, Jennifer Blain Christen, Karen Anderson, Benjamin Katchman
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Publication number: 20170059564Abstract: The invention concerns a test element for carrying out an immunological sandwich test for determining an analyte from a liquid sample containing a reagent zone or conjugate zone which contains a conjugate of an analyte binding partner and a label which can be detected directly or indirectly by visual, optical or electrochemical means (e.g. an enzyme, fluorescent or direct label etc.) wherein said conjugate can be dissolved by the liquid sample, a detection zone which contains a permanently immobilized (i.e. which cannot be detached by the liquid sample) binding partner for the analyte or for complexes containing the analyte; and a control zone which contains a permanently immobilized binding partner for the conjugate of analyte binding partner and label characterized in that the control zone additionally contains one or more permanently immobilized binding partner(s) for the analyte or for complexes containing the analyte.Type: ApplicationFiled: November 10, 2016Publication date: March 2, 2017Inventor: Juergen Klepp
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Publication number: 20170059565Abstract: Intramolecular biosensors are disclosed, including PBP-based biosensors, comprising a ligand binding domain fused to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding ligand. At least one of the donor and fluorescent moieties may be internally fused to the biosensor such that both ends of the internally fused fluorophore are fixed. In addition, methods of improving the sensitivity of terminally fused biosensors are provided. The biosensors of the invention are useful for the detection and quantification of ligands in vivo and in culture.Type: ApplicationFiled: February 18, 2016Publication date: March 2, 2017Inventors: Wolf B. Frommer, Sakiko Okumoto, Loren Looger, Marcus Fehr
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Publication number: 20170059566Abstract: A device for determining presence or absence of infection due to an infectious agent is described. The device comprises a sample receiving zone configured to receive a liquid sample from a subject suspected of having an infection due to an infectious agent, the sample receiving zone positioned to distribute the sample along a first fluid flow path to a first label zone and along a second fluid flow path to a second label zone. Test lines in each fluid flow path capture a mobile detectable species as an indicator of presence or absence of the infectious agent. The device also comprises a reference line positioned in the one of the fluid flow paths. The bidirectional fluid flow paths emanate from a common sample zone and provide an efficient approach to detection and differentiate two species as indicators of the same infectious agent or as indicators of two different infectious agents.Type: ApplicationFiled: August 25, 2016Publication date: March 2, 2017Inventors: Robert Reed, Irene Sinn, Robert Weiller, Jason McClure
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Publication number: 20170059567Abstract: Methods of electrochemically detecting or quantifying an analyte by coupling a plurality of redox-active agents (e.g., guanine-rich oligonucleotides) to the analyte are disclosed. More particularly, this application discloses affinity-based methods for isolating one or more analytes from a sample and subsequently detecting or determining the concentration of the one or more analytes. Detecting or determining the concentration of one or more analytes may involve measuring the extent of oxidation of guanine nucleobases that have been or are coupled to the analyte.Type: ApplicationFiled: October 7, 2014Publication date: March 2, 2017Inventors: Himanshu Jayant Sant, Bruce K. Gale, Harikrishnan Jayamohan, Christopher J. Lambert, John Minson
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Publication number: 20170059568Abstract: The invention relates to a method to predict the onset and/or development of PML in a patient or in an immunosuppressed patient by detection of JCV specific CTL's in blood of said patient using any of the ten (10) JCV amino acid sequences SEQ ID NO 1-10 separately or in combination with each other.Type: ApplicationFiled: February 19, 2015Publication date: March 2, 2017Inventor: Lieven Jozef Stuyver
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Publication number: 20170059569Abstract: Methods of determining whether a circulating tumor cell (CTC) marker, e.g., a CTC or fragment thereof, is present in a sample, such as a blood sample, are provided. Aspects of the methods include flow cytometrically assaying a fluorescently labeled sample that has been fluorescently labeled with a fluorescently labeled chlorotoxin binding member to determine whether a CTC marker is present in the sample. Also provided are compositions for practicing the methods. The methods and compositions find use in a variety of applications.Type: ApplicationFiled: August 12, 2016Publication date: March 2, 2017Inventor: Margaret Inokuma
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Publication number: 20170059570Abstract: A method for assessing sarcoma metastasis comprising obtaining a biological sample of the subject, detecting a level of a N-terminal segment of moesin in the biological sample, wherein the level of the N-terminal segment of moesin detected in the biological sample of the subject is indicative of the subject developing or with an increased probability of developing metastasis of sarcoma. The present application also provides a biomarker and a kit for assessing metastasis of sarcoma, and uses of such biomarker. The present application also provides a method of treating sarcoma metastasis.Type: ApplicationFiled: April 22, 2015Publication date: March 2, 2017Inventors: Yue ZHANG, Jun BAO
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Publication number: 20170059571Abstract: Methods of diagnosis and/or prognosis of malignant disease from pleural effusion samples are disclosed. The methods typically include determining the normalized intensity of one or more biomarkers, such as acetone and 3-hydroxybutyate, in pleural effusion samples using nuclear magnetic resonance (NMR), such as proton NMR (1H-NMR) spectroscopy. Reference values for malignant disease diagnosis using the disclosed biomarkers are provided. Reference values for prognosis of median survival time based on the disclosed biomarkers are also provided.Type: ApplicationFiled: August 31, 2015Publication date: March 2, 2017Inventor: Ching-Wan Lam
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Publication number: 20170059572Abstract: The present invention provides that exosomes from human body fluid samples contain double stranded genomic DNA that spans all chromosomes and may be used to determine the mutation status of genes of interest in diseases, such as cancer. Furthermore, the present invention provides the use of exosomes to produce therapeutic proteins and for their use in therapy as well as the detection of cancer cell-derived exosomes to diagnose cancer and monitor therapeutic response.Type: ApplicationFiled: December 4, 2014Publication date: March 2, 2017Applicant: Board of Regents, The University of Texas SystemInventors: Raghu KALLURI, Sónia MELO
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Publication number: 20170059573Abstract: A method of detecting peroxynitrite in a sample is described comprising the steps of: (a) providing a complex of a saccharide with an aryl boronate compound of formula (I): Fp-L1-Z-L2-Ar—B(OH)2 (I) wherein: Fp comprises a fluorophore; L1 and L2 are linker groups; Z is a fluorescence switch; and Ar is optionally substituted aryl; (b) contacting said aryl boronate-saccharide complex with said sample, whereby peroxynitrite in said sample cleaves said aryl boronate-saccharide complex to produce a compound of formula (II): F-L1-Z-L2-Ar—OH (II); and (c) detecting a decrease in a fluorescence intensity of said fluorophore resulting from said cleavage reaction in step (b). Peroxynitrite reacts quantitatively, rapidly, and selectively in step (b) of the reaction, whereby medical conditions associated with elevated peroxynitrite can be diagnosed. Also provided are compounds of formula (I) for use in the methods.Type: ApplicationFiled: February 24, 2015Publication date: March 2, 2017Applicant: UNIVERSITY OF BATHInventors: Tony D. JAMES, Steven D BULL, Xiaolong SUN
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Publication number: 20170059574Abstract: The present disclosure provides analyte-specific binding reagents conjugated with a platinum-containing moiety, e.g., cisplatin, and methods, compositions, and kits for their production and use in assays for analyte detection.Type: ApplicationFiled: August 8, 2016Publication date: March 2, 2017Inventors: Holden Terry Maecker, Michael Duane Leipold, Henrik Mei
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Publication number: 20170059575Abstract: Compositions are provided comprising water-stable semi-conductor nanoplatelets encapsulated in a hydrophilic coating further comprising lipids and lipoproteins. Uses include biomolecular imaging and sensing, and methods of making comprise: colloidal synthesis of CdSe core NPLs; layer-by-layer growth of a CdS shell; and encapsulation of CdSe/CdScore/shell NPLs in lipid and lipoprotein components through an evaporation-encapsulation using zwitterionic phospholipids, detergents, and amphipathic membrane scaffold proteins.Type: ApplicationFiled: August 23, 2016Publication date: March 2, 2017Inventors: Andrew Smith, Sung Jun Lim, Aditi Das
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Publication number: 20170059576Abstract: The present invention relates to a method of determining a protein in a sample and the use of the method for the identification of aptamer target proteins.Type: ApplicationFiled: September 7, 2016Publication date: March 2, 2017Inventors: Michael FAMULOK, Gunter MAYER, Jan VINKENBORG