Patents Issued in July 6, 2017
  • Publication number: 20170191054
    Abstract: A method is provided for extracting and purifying components of biological samples with a two-step process for elution and neutralization of the components from the sample. The separate elution and neutralization steps use adjustment of the buffer pH to improve extraction and purification of the desired components.
    Type: Application
    Filed: March 17, 2017
    Publication date: July 6, 2017
    Applicant: Becton, Dickinson and Company
    Inventors: Matthew P. Collis, Michael Justin Lizzi
  • Publication number: 20170191055
    Abstract: This disclosure relates to a method of generating conditionally active biologic proteins from wild type proteins, in particular therapeutic proteins, which are reversibly or irreversibly inactivated at some physiological conditions. For example, conditionally active biologic proteins are active in tumors, but virtually inactive at other body parts, or conditionally active antibodies cap able of crossing blood-brain-barrier.
    Type: Application
    Filed: May 11, 2015
    Publication date: July 6, 2017
    Applicant: BioAtla, LLC
    Inventors: Jay M. Short, Hwai Wen Chang, Gerhard Frey
  • Publication number: 20170191056
    Abstract: Provided herein are monoliths with attached recognition compounds which selectively bind ligands, methods of preparing such monoliths, arrays thereof and uses thereof. For example, monoliths provide herein can be used in columns and arrays thereof.
    Type: Application
    Filed: March 8, 2017
    Publication date: July 6, 2017
    Inventors: Pehr Harbury, Madan Paidhungat, Robin Prince
  • Publication number: 20170191057
    Abstract: Compositions and methods for expanding CD34+ cells, performing research related to cancer stem cells, RNA-editing enzymes and for monitoring, diagnosing and treating, ameliorating and preventing diseases such as cancers or inflammatory diseases.
    Type: Application
    Filed: February 5, 2015
    Publication date: July 6, 2017
    Inventors: Catriona H. Jamieson, Qingfei Jiang, Leslie Crews Robertson, Maria Anna Zipeto, Anil Sadarangani, Gabriel Pineda, Kathleen M. Kane
  • Publication number: 20170191058
    Abstract: Perforin-2 (P2) is expressed by fibroblasts, microglia and macrophages and was found to be responsible for killing bacteria, for example, Mycobacteria smegmatis, M. avium, Salmonellae, MRSA (drug resistant Stapholococci), E. coli. Compounds identified by screening assays are selected based on the effects of these compounds on P2. Use of these compounds in the treatment of infectious diseases, in particular, bacteria and antibiotic-resistant bacteria is also provided.
    Type: Application
    Filed: September 1, 2016
    Publication date: July 6, 2017
    Inventors: Lesley De Armas, Kirill Lyapichev, Ryan McCormack, Eckhard Podack
  • Publication number: 20170191059
    Abstract: The present invention relates to nucleic acid sequences which are fragments of HIV-1 genome. The nucleic acids of the present invention are use as decoys for the treatment of lentivirus infection. In particular, the present invention relates to nucleic acids capable of forming at least one G-quadruplex domain and that are capable of inhibiting the replication of at least one lentivirus, such as HIV-1.
    Type: Application
    Filed: May 22, 2015
    Publication date: July 6, 2017
    Inventors: Samir AMRANE, Jean-Louis MERGNY, Marie-Aline ANDREOLA, Amina BEDRAT
  • Publication number: 20170191060
    Abstract: The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules such as ricin, particularly to ricin B chain.
    Type: Application
    Filed: January 6, 2017
    Publication date: July 6, 2017
    Inventor: George W. Jackson
  • Publication number: 20170191061
    Abstract: The disclosure teaches antagonists of Hepatitis C Virus (HCV) derived microRNA, which are useful in methods for treating and protecting against HCV-mediated hepatocellular carcinogenesis.
    Type: Application
    Filed: March 25, 2015
    Publication date: July 6, 2017
    Inventor: Ajit KUMAR
  • Publication number: 20170191062
    Abstract: The invention provides compositions of oligonucleotides targeted at influenza genes and at host animal genes involved in response to influenza infection. In some embodiments, the oligonucleotides are modified. In some embodiments, the compositions contain one, or more than one, oligonucleotide. The invention also provides methods and kits using the compositions of the invention for the treatment and prevention of influenza.
    Type: Application
    Filed: January 6, 2017
    Publication date: July 6, 2017
    Inventors: Roderic M.K. Dale, Lun-Quan Sun
  • Publication number: 20170191063
    Abstract: The present invention relates to methods and compositions for treating and/or preventing allergic diseases or conditions by inhibiting one or more components of the steroidogenic pathway.
    Type: Application
    Filed: November 10, 2016
    Publication date: July 6, 2017
    Inventors: Erwin W. Gelfand, Meiqin Wang, Yi Jia
  • Publication number: 20170191064
    Abstract: The present invention relates to oligonucleotides that are capable of inducing expression of ubiquitin-protein ligase E3A (UBE3A) from the paternal allele in animal or human neurons. The oligonucleotides target the suppressor of the UBE3A paternal allele by hybridization to SNHG14 long non-coding RNA downstream of SNORD109B. The present invention further relates to pharmaceutical compositions and methods for treatment of Angelman syndrome.
    Type: Application
    Filed: November 14, 2016
    Publication date: July 6, 2017
    Inventors: Veronica Costa, Maj HEDTJÄRN, Marius Hoener, Ravi Jagasia, Mads Aaboe Jensen, Christoph Patsch, Lykke Pedersen, Søren Vestergaard Rasmussen
  • Publication number: 20170191065
    Abstract: A method of enhancing resistance of a mosquito to a pathogen is disclosed. The method comprising administering to a mosquito an isolated nucleic acid agent comprising a nucleic acid sequence which specifically downregulates an expression of at least one mosquito or pathogen gene wherein a product of said mosquito or pathogen gene participates in infection and/or growth of the pathogen in the mosquito.
    Type: Application
    Filed: May 4, 2015
    Publication date: July 6, 2017
    Inventors: Nitzan PALDI, Humberto BONCRISTIANI JUNIOR, Eyal MAORI, Avital WEISS, Emerson Soares BERNARDES
  • Publication number: 20170191066
    Abstract: The present invention provides compounds comprising oligonucleotides complementary to an LRP8 transcript. Certain such compounds are useful for hybridizing to an LRP8 transcript, including but not limited, to an LRP8 transcript in a cell. In certain embodiments, such hybridization results in modulation of splicing of the LRP8 transcript. In certain embodiments, such hybridization results in an increase in inclusion of exon 19 in the LRP8 mRNA transcript. In certain embodiments, such compounds are used to treat Alzheimer's Disease.
    Type: Application
    Filed: June 4, 2015
    Publication date: July 6, 2017
    Applicants: Ionis Pharmaceuticals, Inc., Rosalind Franklin University of Medicine and Science
    Inventors: Frank Rigo, Michelle L. Hastings
  • Publication number: 20170191067
    Abstract: Methods and compositions are disclosed for controlling expression of TNF receptors (TNFR1 and TNFR2) and of other receptors in the TNFR superfamily using compounds that modulate splicing of pre-mRNA encoding these receptors. More specifically these compounds cause the removal of the transmembrane domains of these receptors and produce soluble forms of the receptor which act as an antagonist to reduce TNF-? activity or activity of the relevant ligand. Reducing TNF-? activity provides a method of treating or ameliorating inflammatory diseases or conditions associated with TNF-? activity. Similarly, diseases associated with other ligands can be treated in like manner. In particular, the compounds of the invention are splice-splice switching oligomers (SSOs) which are small molecules that are stable in vivo, hybridize to the RNA in a sequence specific manner and, in conjunction with their target, are not degraded by RNAse H.
    Type: Application
    Filed: January 17, 2017
    Publication date: July 6, 2017
    Inventors: Peter L. Sazani, Ryszard Kole, Henrik Orum
  • Publication number: 20170191068
    Abstract: In one aspect, the invention relates to methods and compositions for determining migration of a virus particle to the nucleus of a cell. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.
    Type: Application
    Filed: December 23, 2016
    Publication date: July 6, 2017
    Inventors: Mark Spear, Yuntao Wu
  • Publication number: 20170191069
    Abstract: The present invention provides methods to assemble and fuse a full length Abortive Promoter Cassette (APC) to a target nucleic acid during PCR amplification of the target. The linked APC is used to quantify amplicon abundance by the production of RNA Abscripts from the synthetic APC. Stepwise PCR-dependent promoter assembly allows for target-fusion of APCs that are too long to be synthesized as monolithic promoter-primer oligonucleotide reagents.
    Type: Application
    Filed: January 3, 2017
    Publication date: July 6, 2017
    Inventors: David McCarthy, Michelle M. Hanna
  • Publication number: 20170191070
    Abstract: This invention provides high growth capacity strains of auxotrophic Escherichia coli and methods for generating thereof. The high growth capacity strains express a complementing auxotrophic plasmid that allows the strain to grow in the absence of the auxotrophic amino acid. Also, provided herein is a method for preparing a bacterial cell extract of a high growth capacity strain of auxotrophic Escherichia coli for use in an in vitro protein expression.
    Type: Application
    Filed: June 30, 2015
    Publication date: July 6, 2017
    Inventors: Dan Groff, Patrick Rivers, Stuart Bussell, Alexander Steiner
  • Publication number: 20170191071
    Abstract: A bacterium producing monophosphoryl lipid A (MLA) comprising a genetic modification that increases expression of a gene encoding LpxE polypeptide and a method of producing MLA are provided. According to the present invention, MLA may be produced in a simple manner without acid hydrolysis and/or base hydrolysis.
    Type: Application
    Filed: December 14, 2016
    Publication date: July 6, 2017
    Inventors: Hak Suk CHUNG, Eun Gyeong YANG, Dohyeon HWANG
  • Publication number: 20170191072
    Abstract: A strain has a microorganism fertilizer efficacy and can be used as a biopesticide, by isolating multi-functional plant endophytic bacteria, which can widely suppress the growth of plant-pathogenic fungi and bacteria and specifically act on rice as a host plant, thereby retaining both a disease resistance inducing effect and a plant growth promoting effect, followed by mass-culturing and formulating. The present invention provides novel microorganism Bacillus oryzicola or Bacillus oryzicola having a DNA-DNA relatedness value of 70% or more with the Bacillus oryzicola.
    Type: Application
    Filed: May 28, 2015
    Publication date: July 6, 2017
    Applicant: JGREEN INDUSTRY INC.
    Inventors: Young Ryun CHUNG, Mohammad Tofajjal HOSSAIN, Eu Jin CHUNG, Geun Gon KIM, Jung Eun LEE
  • Publication number: 20170191073
    Abstract: Disclosed are nucleotide sequences and corresponding amino acid sequences of Arxula adeninivorans genes that can be utilized to manipulate the lipid content and/or composition of a cell. Methods and compositions for utilizing this information are disclosed to increase the lipid content or modify the lipid composition of a cell by either increasing or decreasing the activity of certain genetic targets.
    Type: Application
    Filed: May 29, 2015
    Publication date: July 6, 2017
    Inventors: Elena E. Brevnova, Arthur J. Shaw, IV, Johnnes P. Van Dijken
  • Publication number: 20170191074
    Abstract: Methods of providing gene suppression DNA in a eukaryotic organism comprising introducing a first DNA segment and at least one second DNA segment into the genome of the organism. One of the DNA segments contains a promoter and a transcribable DNA. Another DNA segment contains at least part of the transcribable DNA. When inserted in tandem, the DNA segments are assembled in vivo forming a recombinant transcription unit. RNA transcribed from the transcription unit can form double-stranded RNA.
    Type: Application
    Filed: March 22, 2017
    Publication date: July 6, 2017
    Inventors: JoAnne Fillatti, Larry Gilbertson, Toni Voelker
  • Publication number: 20170191075
    Abstract: The present invention relates to the modification of lignin biosynthesis in plants, to enzymes involved in the lignin biosynthetic pathway and nucleic acids encoding such enzymes and, more particularly, to methods of modifying lignin biosynthesis via sense suppression and to related nucleic acids and constructs.
    Type: Application
    Filed: January 12, 2017
    Publication date: July 6, 2017
    Applicants: Dairy Australia Limited, Agriculture Victoria Services Pty Ltd
    Inventors: German Spangenberg, Angela Jane Lidgett, Robyn Louise Heath, Russell Leigh McInnes, Damian Paul Lynch, Ulrik Peter John, Aidyn Mouradov, Megan Elizabeth Griffith
  • Publication number: 20170191076
    Abstract: Cotton event pDAB4468.19.10.3 comprises genes encoding AAD-12 and PAT, affording herbicide tolerance to cotton crops containing the event, and enabling methods for crop protection.
    Type: Application
    Filed: March 7, 2017
    Publication date: July 6, 2017
    Inventors: Yunxing C. Cui, Raina King, Tina M. Kaiser, Andrew E. Robinson, Dayakar Pareddy, Sandra G. Toledo, Leon B. Braxton, David M. Anderson, Terry R. Wright
  • Publication number: 20170191077
    Abstract: The invention relates to a plant that includes a transgene encoding a heterologous polypeptide conferring on plant expressing said polypeptide resistance to a hemipteroid sap-sucking insect. The transgene is also expressed in a plant component (such as a leaf). Typically, expression of such polypeptides deters feeding by insects such as psyllids (such as an Asian citrus psyllid, the African citrus psyllid, or the American citrus psyllid). Exemplary plants useful in the invention are citrus or solanaceous plants.
    Type: Application
    Filed: June 4, 2015
    Publication date: July 6, 2017
    Inventors: Herb ALDWINCKLE, Kerik COX, Charles LINN, Ewa BOREJSZA-WYSOCKA, Jean-Michel HILY, Dong H. CHA
  • Publication number: 20170191078
    Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
    Type: Application
    Filed: August 5, 2016
    Publication date: July 6, 2017
    Inventors: Feng ZHANG, Le CONG, Fei RAN
  • Publication number: 20170191079
    Abstract: A method of correcting singletons in a selected AAV sequence in order to increasing the packaging yield, transduction efficiency, and/or gene transfer efficiency of the selected AAV is provided. This method involves altering one or more singletons in the parental AAV capsid to conform the singleton to the amino acid in the corresponding position(s) of the aligned functional AAV capsid sequences.
    Type: Application
    Filed: January 18, 2017
    Publication date: July 6, 2017
    Inventors: Luk Vandenberghe, Guangping Gao, James M. Wilson
  • Publication number: 20170191080
    Abstract: The present invention relates to a method for solid phase transfection of at least one protein and at least one nucleic acid molecule into at least one cell comprising the step of providing a solid carrier containing at least one spot comprising a transfection reagent, at least one protein and the at least one nucleic acid molecule as well as to a solid carrier containing at least one spot comprising a transfection reagent, at least one protein and the at least one nucleic acid molecule.
    Type: Application
    Filed: July 14, 2014
    Publication date: July 6, 2017
    Inventors: Holger ERFLE, Vytaute STARKUVIENE-ERFLE, Ruben BULKESCHER
  • Publication number: 20170191081
    Abstract: The invention relates to a method for the simultaneous integration of two or more copies of a polynucleotide of interest into the chromosome of a fungal host cell comprising at least two pairs of recognition sequences of a site-specific recombinase, each pair flanking a resident negative selection marker; transformation of the cell with a construct carrying a gene of interest also flanked by the recognition sequences to ensure double-crossover events after transient expression of the recombinase, followed by selection for excision of all negative selection markers from the cell.
    Type: Application
    Filed: January 17, 2017
    Publication date: July 6, 2017
    Applicant: Novozymes A/S
    Inventor: Hiroaki Udagawa
  • Publication number: 20170191082
    Abstract: The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.
    Type: Application
    Filed: March 10, 2017
    Publication date: July 6, 2017
    Inventors: Fuqiang CHEN, Gregory D. DAVIS
  • Publication number: 20170191083
    Abstract: A method of synthesizing biogenic elemental selenium nanostructure using Enterobacter cloacae and its application. The method uses E. cloacae Z0206 to reduce selenite to zero valence selenium and forms nano-sized elemental selenium particles, including steps of inoculating activated E. cloacae Z0206 to fermentation broth, adding sodium selenite solution, shaking and incubating, collecting the fermentation broth and separating the elemental selenium nanoparticles.
    Type: Application
    Filed: January 3, 2017
    Publication date: July 6, 2017
    Inventors: Yizhen Wang, Deguang Song, Zeqing Lu, Fengqin Wang, Yuanzhi Cheng, Xiaoxiao Li
  • Publication number: 20170191084
    Abstract: The present invention relates to the biological production of methane (Biogas) by co-culture in an aerobic atmosphere of a methanogenic bacterium and of an anaerobic bacterium capable of producing hydrogen, in a culture medium comprising or being supplemented with carbohydrate compound(s), notably starch and/or sugars, and supplemented with antioxidant compound(s).
    Type: Application
    Filed: April 20, 2015
    Publication date: July 6, 2017
    Applicants: FONDATION MEDITERRANEE INFECTION, UNIVERSITE D'AIX MARSEILLE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE)
    Inventors: Didier RAOULT, Saber KHELAIFIA, Michel DRANCOURT
  • Publication number: 20170191085
    Abstract: The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate. The organism can further contain a hydrogen synthesis pathway. The invention additionally provides methods of using such microbial organisms to produce butadiene or 2,4-pentadienoate by culturing a non-naturally occurring microbial organism containing butadiene or 2,4-pentadienoate pathways as described herein under conditions and for a sufficient period of time to produce butadiene or 2,4-pentadienoate. Hydrogen can be produced together with the production of butadiene or 2,4-pentadienoate.
    Type: Application
    Filed: July 2, 2015
    Publication date: July 6, 2017
    Applicant: Genomatica, Inc.
    Inventors: Priti PHARKYA, Anthony P. BURGARD, Mark J. BURK
  • Publication number: 20170191086
    Abstract: A process for transforming essential oils comprising linalool and the production of mixtures of linalool derivatives useful as fragrances in cosmetic compositions.
    Type: Application
    Filed: December 30, 2015
    Publication date: July 6, 2017
    Inventors: Renata BUELONI, Renata Rabelo SCHEFER, Roberta ROESLER
  • Publication number: 20170191087
    Abstract: The present invention relates to the use of GLO1 to modulate acetic acid tolerance in yeast. More specifically, it relates to the use of a specific GLO1 allele to confer tolerance to acetic acid, and to improve the fermentation performance of yeast in the presence of acetic acid.
    Type: Application
    Filed: May 26, 2015
    Publication date: July 6, 2017
    Inventors: Johan Thevelein, Maria Remedios Foulquié Moreno, Meijnen Jean-Paul
  • Publication number: 20170191088
    Abstract: The present invention provides a microorganism capable of fermenting arabinose to a desired product such as ethanol. In some embodiments, the organism is also capable of fermenting xylose. In some embodiments, the organism is capable of fermenting arabinose and xylose, and expresses one or more cellulases.
    Type: Application
    Filed: March 9, 2017
    Publication date: July 6, 2017
    Inventors: D. Aaron Argyros, Nicky Caiazza, Trisha F. Barrett, Anne K. Warner
  • Publication number: 20170191089
    Abstract: The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: —a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and —a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate.
    Type: Application
    Filed: May 28, 2015
    Publication date: July 6, 2017
    Applicant: DSM IP Assets B.V.
    Inventors: Zheng ZHAO, Bernard MEIJRINK, Robertus Antonius Mijindert VAN DER HOEVEN, Liang WU, Johannes Andries ROUBOS
  • Publication number: 20170191090
    Abstract: Processes for starting up and operating anaerobic, deep tank fermentation reactors including a process for anaerobic bioconversion of a gas substrate comprising carbon monoxide, hydrogen, and carbon dioxide in a reactor by contact of the gas substrate with an aqueous menstruum containing microorganisms suitable for converting the gas substrate to an oxygenated organic compound in the reactor. The process further includes: blanketing the reactor above the aqueous menstruum to the essential exclusion of oxygen with a head space gas comprising at least one of carbon monoxide, hydrogen, carbon dioxide, nitrogen, and a lower alkane; continuously supplying a feed gas comprising at least a portion of the gas substrate to the aqueous menstruum in the reactor; and injecting the gas substrate and a motive liquid into the reactor to form a dispersion of the motive liquid and microbubbles, the microbubbles having a diameter of less than about 500 microns.
    Type: Application
    Filed: March 21, 2017
    Publication date: July 6, 2017
    Inventors: Robert Hickey, Richard E. Tobey, Shih-Perng Tsai
  • Publication number: 20170191091
    Abstract: The present invention relates to a recombinant cell which is capable of producing one or more of itaconic acid, 4-methyl itaconate or 1-methyl itaconate, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. A recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: —a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and —one or more nucleic acids encoding polypeptides which separately or together catalyze a reaction towards acetyl CoA, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.
    Type: Application
    Filed: May 28, 2015
    Publication date: July 6, 2017
    Inventors: Zheng ZHAO, Bernard MEIJRINK, Robertus Antonius Mijndert VAN DER HOEVEN, Liang WU, Johannes Andries ROUBOS, Marina VAN GELDER
  • Publication number: 20170191092
    Abstract: Described herein are genetically-modified microorganisms for producing medium chain lauric acid and/or dodecanedioic acid and methods of using the microorganisms. The microorganisms contain a nucleic acid encoding an Umbellularia californica lauroyl ACP-thioesterase (BTE) operably linked to a promoter.
    Type: Application
    Filed: December 29, 2016
    Publication date: July 6, 2017
    Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
    Inventors: Hsin-Ju HSIEH, Yu-Ju LIN, Hwei-Jiung WANG, Chwan-Deng HSIAO, Cheng-Chung LEE, Ya-Wen CHEN
  • Publication number: 20170191093
    Abstract: Disclosed are methods and compositions for increasing the triacylglycerol content of a cell by increasing the activity of a type 1 diacylglycerol acyltransferase (i.e., DGA2) and increasing the activity of a type 2 diacylglycerol acyltransferase (i.e., DGA1). In some embodiments, the triacylglycerol content of a cell is also modified by decreasing the activity of a triacylglycerol lipase in the same cell. Also disclosed are methods and compositions for increasing the triacylglycerol content of a cell by increasing the activity of a type 1 diacylglycerol acyltransferase (i.e., DGA2), or by increasing the activity of a type 3 diacylglycerol acyltransferase (i.e., DGA3).
    Type: Application
    Filed: May 29, 2015
    Publication date: July 6, 2017
    Inventors: Vasiliki Tsakraklides, Elena E. Brevnova
  • Publication number: 20170191094
    Abstract: The present invention provides recombinant algae expressing exogenous Type I fatty acid synthase (FAS) genes and demonstrating higher rates of fatty acid synthesis with respect to control microorgansims. The recombinant algae are able to produce greater amounts of FAME lipids under nitrogen replete conditions.
    Type: Application
    Filed: December 29, 2016
    Publication date: July 6, 2017
    Inventors: Eric Moellering, Weidong Xu, John H. Verruto, Paul Gordon Roessler
  • Publication number: 20170191095
    Abstract: Described is a method for the production of D-erythrose and acetyl phosphate comprising the enzymatic conversion of D-fructose into D-erythrose and acetyl phosphate by making use of a phosphoketolase. The produced D-erythrose can further be converted into glycolaldehyde by a method for the production of glycolaldehyde comprising the enzymatic conversion of D-erythrose into glycolaldehyde by making use of an aldolase, wherein said aldolase is a 2-deoxyribose-5-phosphate aldolase (EC 4.1.2.4) or a fructose-bisphosphate aldolase (EC 4.1.2.13). The produced glycolaldehyde can finally be converted into acetyl phosphate by the enzymatic conversion of the thus produced glycolaldehyde into acetyl phosphate by making use of a phosphoketolase or a sulfoacetaldehyde acetyltransferase.
    Type: Application
    Filed: May 22, 2015
    Publication date: July 6, 2017
    Inventor: Philippe Marliere
  • Publication number: 20170191096
    Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the carbon chain elongation enzymes or homologs thereof associated with the cyclohexane carboxylate biosynthesis from Syntrophus aciditrophicus or 2-aminoadipate lysine biosynthesis.
    Type: Application
    Filed: March 15, 2017
    Publication date: July 6, 2017
    Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
  • Publication number: 20170191097
    Abstract: Isolated oxidases, isolated polynucleotides encoding the oxidases, and methods of using the oxidases to produce ?-oxocarboxylic acid compounds or L-?-amino acid compounds are described.
    Type: Application
    Filed: July 2, 2015
    Publication date: July 6, 2017
    Applicants: Sumitomo Chemical Company, Limited, Sumitomo Chemical Company, Limited
    Inventor: Hiroyuki ASAKO
  • Publication number: 20170191098
    Abstract: The present invention relates to improving the enzymatic activity of Escherichia coli O157:H7-derived sugar isomerase by molecular mechanics and site-directed mutagenesis. More particularly, the present invention relates to a sugar isomerase having an enzymatic activity improved by mutation, a nucleic acid molecule encoding the same, a vector comprising the nucleic acid molecule, a transformant comprising the vector, a mutant of the sugar isomerase, and a method for producing L-gulose using the improved sugar isomerase.
    Type: Application
    Filed: June 13, 2014
    Publication date: July 6, 2017
    Inventors: Jung-Kul LEE, Chandra Sujan SIGDEL, Jinglin LI, Tae-Su KIM
  • Publication number: 20170191099
    Abstract: A system and method to treat biomass to make bioproducts such as biofuels and biochemical is disclosed. The systems are configured to treat a liquid component separated from biomass to yield sugars available to form a fermentation product or bio-product. The system and method comprises treatment and separation of process streams (e.g. liquids, solids, etc.) to produce bioproducts such as fuel and biochemical as well as to recover/reuse and utilize content of process streams. Lignin is separated from the bioproduct stream (e.g. a solids component) forming a recovered bioproducts; efficient removal of lignin facilitates productive operation of the system. The system disclosed is configured to recover and recycle acid (e.g. phosphoric acid) used as a concentrated acid in the process to pre-treat biomass (for bioprocessing). The acid used in the process is recycled and recovered in order to establish economically attractive biorefinery to make bio-products from biomass (e.g.
    Type: Application
    Filed: December 4, 2016
    Publication date: July 6, 2017
    Applicant: Arbiom Inc.
    Inventors: William F. McDonald, Naga Vinod Kumar Bathula, Michael Bowyer, Brandon Kiser, Vaishnavi Srinivasan, Lisette Tenlep, Brie-Anna Walker
  • Publication number: 20170191100
    Abstract: [Problem] To provide a bacterial cellulose which is highly dispersible in a liquid, shows excellent molding properties and high miscibility with other materials when applied to materials, and, therefore, has a high applicability as a practical material, and a bacterium which produces the bacterial cellulose. [Solution] A bacterial cellulose, water that contains said bacterial cellulose at a final concentration of 0.1±0.006 (w/w) showing a light transmittance at a wavelength of 500 nm of 35% or greater, and a bacterium producing the bacterial cellulose. According to the present invention, the bacterial cellulose that is uniformly dispersible in a liquid such as water can be obtained. The bacterial cellulose shows excellent molding properties and high miscibility with other materials and, therefore, can contribute to the improvement in the qualities of a final product or production efficiency thereof or to the reduction of production cost.
    Type: Application
    Filed: February 16, 2017
    Publication date: July 6, 2017
    Inventors: Kenji Tajima, Ryota Kose
  • Publication number: 20170191101
    Abstract: Disclosed is an improved method for clarifying a cell culture during the manufacture of a protein. The method includes the step of transiently reducing the pH of a cell culture, followed by a holding step for a period of time, followed by the neutralization of the cell culture prior to clarification by centrifugation.
    Type: Application
    Filed: March 17, 2015
    Publication date: July 6, 2017
    Inventors: Benjamin ADAMS, Andrew TUSTIAN, Hanne BAK
  • Publication number: 20170191102
    Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.
    Type: Application
    Filed: September 16, 2016
    Publication date: July 6, 2017
    Inventors: Meixia ZHOU, Bradley Richard SNEDECOR, Chi Kin Domingos NG, Amy SHEN
  • Publication number: 20170191103
    Abstract: The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.
    Type: Application
    Filed: December 20, 2016
    Publication date: July 6, 2017
    Applicant: National University Corporation Chiba University
    Inventors: Daisuke Umeno, Maiko Furubayashi, Norihiko Misawa, Shinichi Takaichi