Patents Issued in July 6, 2017
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Publication number: 20170191004Abstract: The present invention relates to an improved detergent composition for use in the protection of non-metallic inorganic materials such as glassware in automatic ware washing machines.Type: ApplicationFiled: June 1, 2015Publication date: July 6, 2017Inventors: Claudia Schmaelzle, Pavlinka Roy
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Publication number: 20170191005Abstract: The present invention relates to an aqueous liquid laundry formulation comprising an ester based laundry ingredient; an effective cleaning amount of protease enzyme; an effective cleaning amount of lipase enzyme; and from 5 to 60 wt % surfactant; wherein at least 70 wt % of the effective cleaning amount of lipase enzyme is encapsulated and separated from the ester based laundry ingredient and the liquid by a coating which is insoluble in the formulation but which dissolves on dilution with the wash; and wherein the laundry formulation comprises at least 20 wt % water.Type: ApplicationFiled: May 18, 2015Publication date: July 6, 2017Inventors: Morten FOVERSKOV, David Stephen GRAINGER, Henrik LUND, John Francis WELLS
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Publication number: 20170191006Abstract: A method is provided for cleaning a system by conducting continuously through the system a cleaning composition including at least one oxidizing agent and an indicator for detecting the cleanliness of the system by observation of a color change of the indicator. Color values are determined at one or more points and compared with a setpoint value. Color values F are determined at fixed time intervals after exit of the composition from the system; differences ?F are formed from two color successive values; color values are determined before commissioning of the clean system until the difference ?F=0, after which the color value measured last is defined as an inherent system value FA and a maximum tolerable deviation from this value is fixed as a setpoint value ?FA for cleaning; and cleaning of the system is carried out after operation of the system until a difference of ??FA is measured.Type: ApplicationFiled: March 24, 2015Publication date: July 6, 2017Applicant: THONHAUSER GMBHInventors: Daniel HERZOG, Philip THONHAUSER
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Publication number: 20170191007Abstract: The present invention provides a Fischer-Tropsch gasoil fraction having an initial boiling point of at least 165° C. and a final boiling point of at most 220° C. In another aspect the present invention provides a cleaning composition and the use of a Fischer-Tropsch gasoil fraction according to the invention.Type: ApplicationFiled: May 26, 2015Publication date: July 6, 2017Inventors: Johannes Bernardus Wilhelmus MORSINK, Johannes TURFBOER, Rendert Jan WIERSMA, Ferry WINTER
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Publication number: 20170191008Abstract: The present disclosure is directed to compositions including a microemulsion comprising a blend of lecithin, a co-surfactant and a salt of an acidifier, an ester of an acidifier, or combinations thereof. Uses of the compositions are also disclosed.Type: ApplicationFiled: June 5, 2015Publication date: July 6, 2017Inventor: Shireen Baseeth
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Publication number: 20170191009Abstract: The present invention relates to a solid concentrate composition comprising: (a) from 5% to 25% by weight of zinc pyrithione (ZPT); (b) from 8% to 85% by weight of at least one surfactant, preferably, wherein the solid concentrate composition has a penetration hardness of between 20 N and 50 N according to the test method as disclosed herein.Type: ApplicationFiled: September 14, 2015Publication date: July 6, 2017Inventors: Charlie Reyes SALVADOR, Chunpeng JIANG, Yanan SUN, Zhe LIU, Gbenga Segun SHOWOLE, Anurag MAKRANDI, Raul Songco NICDAO
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Publication number: 20170191010Abstract: The present invention discloses a method of preparing a bactericidal, fungicidal and anti-allergenic Zanthoxylum schinifolium skin care soap, wherein the bactericidal, fungicidal and anti-allergenic Zanthoxylum schinifolium skin care soap comprises the following components based on percentage by weight: Component A: Zanthoxylum schinifolium seed oil 15.0-21.0%, propylene glycol 12.0-17.0%, glycerin 6.0-11.0%, Component B: deionized water 5.0-8.0%, sodium alkyl ether sulfate AES 8.0-12.0%, Component C: lauric acid 5.0-8.0%, stearic acid 11.0-16.0%, Component D: 98% NaOH 2.5-4.5%, deionized water balance, Component E: Zanthoxylum schinifolium essential oil 0.3-0.8%. The skin care soap of the present invention is pale yellow and translucent, has good stability, provides great aroma and rich foam, and can nourish the skin and sterilize effectively.Type: ApplicationFiled: January 5, 2017Publication date: July 6, 2017Inventors: CHENGYING SHAN, WEIMING ZHANG, SHIHONG MA, GUOQING CHEN, HAIBO WANG, SHUHONG LI
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Publication number: 20170191011Abstract: A tissue engineered model (TEM) structure, an apparatus and method for making a TEM structure, and methods of using a TEM structure are disclosed. In an embodiment, the TEM structure includes at least one TEM segment. Each TEM segment includes a frame defining a bounded area, the frame having a height, a first edge, and a second edge opposite the first edge, each of the first edge and the second edge defining a perimeter of the bounded area, and the height defining a distance between the first edge and the second edge; a membrane affixed to the first edge about a perimeter of the frame; and a solidified gel and cell matrix disposed within the bounded area within the frame, wherein the solidified gel and cell matrix substantially fills a volume defined by the bounded area and the height of the frame.Type: ApplicationFiled: March 20, 2017Publication date: July 6, 2017Inventors: John G. BAUST, Joshua T. SMITH, Kimberly L. SANTUCCI, Kristi K. SNYDER, Anthony T. ROBILOTTO, Robert G. VAN BUSKIRK, John M. BAUST, William L. CORWIN, Jennie F. MCKAIN
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Publication number: 20170191012Abstract: A culture flask comprising a culture chamber in a flask body comprising an even bottom wall with a growth surface on the top side, an even cover wall at a section from the bottom wall, and side walls that bridge the distance between the margins of the bottom wall and cover wall, an opening that is in adjacent regions of a side wall and the even cover wall at a distance from the bottom wall, and a hollow cylindrical flask neck that is connected to the margin of the opening and is aligned at a sharp angle to the growth surface so that the serological pipette, a scraper, a cannula or another elongated device for adding or removing material can be placed on the rear corners of the growth surface from the outside through the flask neck.Type: ApplicationFiled: March 23, 2017Publication date: July 6, 2017Applicant: Eppendorf AGInventor: Martin SEIPPEL
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Publication number: 20170191013Abstract: A shallow container for culturing organisms includes a holder defining a cavity and a separate lid to close off the cavity. The lid includes a transparent window portion to be arranged above the cavity when the lid closes off the cavity. The window portion includes an upper surface and a lower surface extending parallel to each other; where the cavity is filled with a matrix having a reflective top surface on which an organism to be cultured can be received, and where in at least one position of the lid with respect to the holder, in which the lid closes off the cavity, the top surface of the matrix extends non-parallel to the upper and lower surfaces of the window portion.Type: ApplicationFiled: May 28, 2015Publication date: July 6, 2017Inventors: Franciscus Henricus Alphonsus Gerardus Fey, Gerard Rudolf Riemens, Edwin Langerak, Marinus Beije
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Publication number: 20170191014Abstract: A system for processing samples by a robotic platform has a tray that defines a well defining a drain. The well has a bottom surface, an access port in fluid communication with the drain, and an insert adapted to be received in the well. The insert has a bottom wall and a side wall. At least one of the bottom wall and the side wall define a plurality of openings, such that an interior of the insert is in fluid communication with the well when the insert is inserted into the well.Type: ApplicationFiled: March 16, 2017Publication date: July 6, 2017Inventors: Rostislav Chernomorsky, Nicholas Gale, Sam Cichon
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Publication number: 20170191015Abstract: The present invention relates to an apparatus comprising a single-use circular bag having a sealed edge, capable of holding a nutrient media and designed to deliver heated or cooled air/gas into the media thereby aerating and maintaining the appropriate temperature for growth of a cell culture. In addition mixing is provided by the use of acoustic radiation devices located below a support structure.Type: ApplicationFiled: January 4, 2016Publication date: July 6, 2017Applicant: THERAPEUTIC PROTEINS INTERNATIONAL, LLCInventor: Sarfaraz K. Niazi
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Publication number: 20170191016Abstract: An adjustable harvest valve assembly for a bioreactor system which offers all of the benefits of a harvest port, while allowing cell-free liquid to be collected at various liquid heights and multiple times throughout the run.Type: ApplicationFiled: December 29, 2016Publication date: July 6, 2017Inventors: Brian Lee, Gary Evans, Tom Zevin, Daniel Giroux, Yas Hashimura, Oscar Garza
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Publication number: 20170191017Abstract: A system and method for monitoring viscosity changes of a fluid stored in a volume are provided. The system includes a flexible chamber configured to receive and hold the fluid, a motion generator configured to induce a wave motion within the fluid, at least one sensor affixed at least in part to a portion of the flexible chamber and configured to measure at least a strain on a portion of the flexible chamber and generate an associated strain output. The strain output is effectuated by the wave motion of the fluid within the flexible chamber and correlates to a viscosity value of the fluid. A computer or controller is configured to receive the strain output from the sensor at a given time, compare the viscosity value associated with the strain output to a reference viscosity value, and determine whether to adjust the wave motion generated by the motion generator.Type: ApplicationFiled: December 30, 2015Publication date: July 6, 2017Inventors: Gregory Heinz, Jimmie Beacham, JR.
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Publication number: 20170191018Abstract: This disclosure describes a biochemical reactor with side nozzles. The biochemical reactor may include a tank configured to house immobilized carriers and fluid. The biochemical reactor may include a circulation conduit at least partially disposed within the tank. The circulation conduit may include a circulation inlet opening and a circulation outlet opening. The biochemical reactor may include one or more vanes disposed proximate to the circulation outlet opening. The one or more vanes may be configured to cause the immobilized carrier and the fluid exiting the circulation outlet opening to enter into a helical pattern. The biochemical reactor may include one or more side nozzles configured to induce a tangential flow of fluid within the tank. The one or more side nozzles may be disposed proximate to the circulation inlet opening and/or the circulation outlet opening.Type: ApplicationFiled: March 23, 2017Publication date: July 6, 2017Inventor: Peter J. Hall
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Publication number: 20170191019Abstract: The present invention addresses the problem of providing: a cell dispersion measurement mechanism whereby it becomes possible to fully disperse cells regardless of the experiences of operators skilled in cell culture and it also becomes possible to determine the number or concentration of cells accurately; a cell culture apparatus equipped with the cell dispersion measurement mechanism; and a cell dispersion measurement method. The problem can be solved by circulating a cell suspension in a flow path to disperse cell masses contained in the cell suspension, and then determining over time the number or concentration of cells and/or the degree of dispersion of cells in the cell suspension that is flowing in the circulation flow path.Type: ApplicationFiled: July 8, 2015Publication date: July 6, 2017Applicant: Hitachi High-Technologies CorporationInventors: Masako KAWARAI, Akihiro SHIMASE, Sadamitsu ASO, Toshinari SAKURAI, Eiichiro TAKADA, Kazumichi IMAI
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Publication number: 20170191020Abstract: A test vessel includes one or more test locations configured to contain a medium suitable for culturing a live substance. A thermochromic material is thermally coupled to the one or more test locations. The thermochromic material is configured to exhibit a spectral shift in light emanating from the thermochromic material in response to an increase or decrease in energy conversion by the live substance that causes a change in temperature of the thermochromic material.Type: ApplicationFiled: December 30, 2015Publication date: July 6, 2017Inventors: Michael I. Recht, Joerg Martini, Peter Kiesel
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Publication number: 20170191021Abstract: A cell culture device and a cell culture method that enable living tissues for transplantation having constant qualities to be stably supplied as products are provided. A plurality of cell culture portions that respectively culture a plurality of cell culture units; a maturity evaluation portion that respectively evaluates maturity of the respective cell culture units in the respective cell culture portions and a movement target cell determining portion that determines a cell culture unit that does not satisfy a predetermined condition as a target to be moved to another cell culture portion in a case where the cell culture unit of which maturity does not satisfy the predetermined condition among a plurality of cell culture units cultured in any one cell culture portion of the plurality of cell culture portions exists are provided.Type: ApplicationFiled: March 17, 2017Publication date: July 6, 2017Applicant: FUJIFILM CorporationInventor: Takashi WAKUI
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Publication number: 20170191022Abstract: A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.Type: ApplicationFiled: March 22, 2017Publication date: July 6, 2017Inventors: Bart Lipkens, Louis Masi, Stanley Kowalski, III, Walter M. Presz, JR., Jason Dionne, Brian Dutra, Ari Mercado, Thomas J. Kennedy, III, Arthur Martin, John Rozembersky
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Publication number: 20170191023Abstract: The present invention is directed to recombinant thraustochytrids that grow on xylose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids.Type: ApplicationFiled: January 6, 2017Publication date: July 6, 2017Inventors: James Casey LIPPMEIER, Kirk E. Apt
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Publication number: 20170191024Abstract: The present invention relates to a method for preparation of a biomass of photosynthetic microalgae having a phycobiliprotein content equal to at least 20% of the dry weight of said biomass. The present invention also relates to a biomass enriched with phycobiliproteins and the use thereof as a food supplement or ingredient for nutraceutical and cosmetic preparations.Type: ApplicationFiled: August 28, 2015Publication date: July 6, 2017Applicant: ALGOBIOTECHInventors: Hatem BEN OUADA, Jihene AMMAR
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Publication number: 20170191025Abstract: The present invention provides a compounded cell culture medium powder formulation comprising: a basal medium powder and a cell culture media supplement, wherein the cell culture media supplement comprises and one or more salts; one or more growth factors; one or more inorganic ions; an amino acid supplement comprising one or more of asparagine, glutamine, histidine, and serine; one or more buffers; and one or more anti-foaming agents. The invention further provides methods of making a compounded cell culture medium powder formulation methods of making a cell culture medium for growing mammalian cells and methods of producing a protein of interest by culturing cells in the cell culture medium and isolating the protein of interest.Type: ApplicationFiled: April 7, 2015Publication date: July 6, 2017Applicant: Bayer HealthCare LLCInventors: Yuval SHIMONI, Volker MÖHRLE
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Publication number: 20170191026Abstract: The invention provides a substrate for cell culture comprising a polymer, wherein the polymer comprises —a homopolymer formed from a monomer of formula (Ia) or (Ib); —a copolymer formed from one or more monomers of formula (Ia) and/or (Ib); or —a copolymer formed from one or more monomers of formula (Ia) and/or (Ib), and comprising HEMA; wherein formula (Ia) comprises: (Formula (Ia)) wherein R1 is a C8-C12 straight or branched chain alkyl or alkenyl group, for example a C8-C10 straight or branched chain alkyl group, which may optionally be substituted; and R2 is selected from H and C1-4 alkyl; and wherein formula (Ib) comprises: (Formula (Ib)) wherein R3 is a 6-12 membered ring, for example a 6-8 membered ring, which is a cycloalkyl, cycloheteroalkyl, aryl or heteroaryl group, and which may optionally be substituted; L is a divalent linker group selected from —NH—, —CH2—, and —O—; R4 is a C1-8 organic group, for example a C1-C6 organic group, wherein this group comprises at least one moiety selected from C?O,Type: ApplicationFiled: September 11, 2015Publication date: July 6, 2017Inventors: Morgan Russell ALEXANDER, Chris DENNING, Lorraine YOUNG, Adam CELIZ, James SMITH, Martyn Christopher DAVIES, Asha PATEL
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Publication number: 20170191027Abstract: The present invention relates to methods and compositions for treating a subject comprising destroying diseased cells in the subject. The methods comprise obtaining a population of cells from a subject and determining the activity of at least one disease marker gene within the population of the obtained cells. A polynucleotide molecule that encodes a polypeptide that is lethal to the cells is then introduced into the cells, where the expression of the lethal polypeptide is controlled by the promoter of at least one of the disease marker genes previously identified. After introduction of the polynucleotide, the cells are treated with conditions to induce expression of the lethal polypeptide to destroy the cells that are expressing the disease marker gene(s). After destruction of the diseased cells, the remaining live cells, which did not express the lethal polypeptide to an extent necessary to kill the cells, are separated from the dead cells, and the live cells are restored to the subject.Type: ApplicationFiled: January 27, 2017Publication date: July 6, 2017Applicant: Intrexon CorportionInventors: Thomas D. REED, Robert P. BEECH
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Publication number: 20170191028Abstract: Methods of developing plant somatic embryos include incubating the plant somatic embryos at a first temperature for a first period of time followed by storing the plant somatic embryos at a second temperature for a second period of time. The first temperature can be in the range of about 23° C. to 30° C. and leads to rapid plant somatic embryo growth. The second temperature can be in the range of about 8° C. to 15° C. and slows plant somatic embryo development. Plant somatic embryos developed in this manner and implanted in artificial seeds show germination rates similar to zygotic embryos implanted in artificial seeds.Type: ApplicationFiled: November 9, 2016Publication date: July 6, 2017Applicant: Weyerhaeuser NR CompanyInventor: Pramod K. GUPTA
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Publication number: 20170191029Abstract: A method for improving quality of a mammalian semen sample includes providing an insoluble carrier carrying at least one ligand that binds to an indicator of impaired sperm membrane integrity and/or motility, or the insoluble carrier carrying at least one molecule capable of binding to the at least one ligand, and contacting the semen sample and the insoluble carrier to provide a bound sperm cell population and an unbound sperm cell population, The insoluble carrier may be a plurality of beads each carrying the at least one ligand or the at least one molecule. The indicator may be associated with one or both of impaired sperm acrosome and impaired sperm plasma membrane integrity. The ligand may be an antibody. The molecule may be one of streptavidin, protein A, and an anti-ligand antibody. Kits for performing the method are provided.Type: ApplicationFiled: January 4, 2017Publication date: July 6, 2017Inventors: J. Lannett Edwards, Louisa A. Rispoli, F. Neal Schrick
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Publication number: 20170191030Abstract: The invention relates to improved culture methods for expanding epithelial stem cells and obtaining organoids, to culture media involved in said methods, and to uses of said organoids.Type: ApplicationFiled: May 15, 2015Publication date: July 6, 2017Applicant: KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPENInventors: Meritxell HUCH ORTEGA, Johannes Carolus CLEVERS, Sylvia FERNANDEZ BOJ
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Publication number: 20170191031Abstract: The present invention relates to compositions and method for differentiating stem cells. In particular, the present invention relates to methods of generating hepatocytes from human pluripotent stem cells (hPSCs) using a small molecule-driven approach.Type: ApplicationFiled: April 30, 2015Publication date: July 6, 2017Inventors: Gareth Sullivan, Sebastian Greenhough
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Publication number: 20170191032Abstract: The disclosure reports on the identification and isolation of adult mouse lateral ventricle subventricular zone (SVZ) neurosphere initiating cells (NICs) by flow cytometry on the basis of GlastmidEGFRhighPlexinB2highCD24?/lowO4/PSA-NCAM?/lowTer-119/CD45? markers (GEPCOT cells). These cells are highly mitotic and short-lived in vivo based on fate-mapping with Ascl1CreERT2 and Dlx1CreERT2. In contrast, pre-GEPCOT cells were quiescent, expressed higher Glast, and lower EGFR and PlexinB2. Pre-GEP-COT cells could not form neurospheres but expressed the stem cell markers Glast-CreERT, GFAP-CreERT2, Sox2CreERT2, and Gli1C-reERT2 and were long-lived in vivo. While GEPCOT NICs were ablated by temozolomide, pre-GEPCOT cells survived and repopulated the SVZ. Conditional deletion of the Bmi-1 polycomb protein depleted pre-GEPCOT and GEPCOT cells, though pre-GEPCOT cells were more dependent upon Bmi-1 for p16Ink4a repression.Type: ApplicationFiled: April 27, 2015Publication date: July 6, 2017Inventors: Sean J. MORRISON, John K. MICH
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Publication number: 20170191033Abstract: An object of the present invention is to provide a method of inducing pluripotent stem cells to differentiate into clinically applicable retinal ganglion cells. The present invention provides a method for inducing pluripotent stem cells to differentiate into retinal ganglion cells that can be used for clinical application. Such method is a method for producing retinal ganglion cells with elongated axons comprising the following steps: (a) a step of inducing pluripotent stem cells to differentiate into retinal progenitor cells via floating culture; (b) a step of inducing the retinal progenitor cells obtained in step (a) to differentiate into retinal ganglion cells via floating culture; and (c) a step of allowing axons to elongate via adhesion culture of the retinal ganglion cells obtained in step (b).Type: ApplicationFiled: August 7, 2015Publication date: July 6, 2017Applicants: NATIONAL CENTER FOR CHILD HEALTH AND DEVELOPMENT, SENJU PHARMACEUTICAL CO., LTD.Inventors: Noriyuki Azuma, Taku Tanaka, Tadashi Yokoi
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Publication number: 20170191034Abstract: The invention concerns a method of preparing a composition comprising stimulated immune system cells such as dendritic cells (DC) for use in inducing immune response of cytotoxic T lymphocytes against colorectal cancer. The dendritic cells are pulsed by contact with colorectal cancer stem cells (CSC) or their fragments thereof. These colorectal CSCs are produced by OSKM (Oct4, Sox2, Klf4 and c-Myc) induced reprogramming of differentiated colorectal cancer cells (CRC) which results in undifferentiated colorectal CSC-like cells. Both the CSC-like cells and the lysates of heat-shocked CSC-like cells could be used as an accessible source of tumour antigens for DC pulsing to induce specific immune responses against colorectal CSCs.Type: ApplicationFiled: September 4, 2015Publication date: July 6, 2017Inventors: Shu WANG, Chunxiao WU, Jieming ZENG, Yovita Ida PURWANTI, Andrew KHOO
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Publication number: 20170191035Abstract: Provided are methods, apparatus, pharmaceutical compositions, and kits for treatment of a metabolic condition, including obesity and type 2 diabetes, by administration to a subject of a therapeutically effective amount of a cell or tissue preparation such as brown adipose microtissues or brown adipose tissue directly converted from white adipose tissue. Modified approaches to creating brown adipose tissue involve differentiation of explanted white adipose tissue and direct browning of white adipose tissue in a bioreactor rather than isolation and expansion of adipose stems cells or endothelial cells and formation and differentiation of 3D cell aggregates.Type: ApplicationFiled: July 10, 2015Publication date: July 6, 2017Applicant: The Trustees of Columbia University in the City of New YorkInventors: Samuel K. SIA, Brian M. GILLETTE
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Publication number: 20170191036Abstract: Disclosed is a method for culturing mesenchymal stem cells, comprising culturing mesenchymal stem cells in a medium containing calcium in a concentration of from 2.1 to 3.8 mM and magnesium in a concentration of from 1.0 to 3.0 mM under a hypoxic condition of 2 to 5% oxygen. The culturing method can increase the population of mesenchymal stem cells even with a small number of passages by improving mesenchymal stem cells in proliferative capacity and viability. In addition, the mesenchymal stem cells prepared by the culturing method are effectively used not only as a safe cell therapeutic agent due to their lacking immunogenicity, but also as a cartilage regenerating medicine owing to their excellent secretion of cytokines.Type: ApplicationFiled: January 11, 2017Publication date: July 6, 2017Applicant: MEDIPOST CO., LTD.Inventors: Yoon-Sun YANG, Won Il OH, Sun Jae KWON, Mi Yeon LEE, Hong Bae JEON
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Publication number: 20170191037Abstract: Provided are a method of culturing an induced pluripotent stem cell and a method of forming a spheroid of the induced pluripotent stem cell. According to an embodiment of the present inventive concept, the induced pluripotent stem cell may have improved stemness.Type: ApplicationFiled: July 29, 2016Publication date: July 6, 2017Inventors: So Yeon KIM, Eun Gyeong YANG, Myung-Han YOON, Hyunju KIM
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Publication number: 20170191038Abstract: Provided is a cocktail of factors for converting/reprogramming non-naïve pluripotent stem cells into TGF? signaling-independent (TSI) naïve induced pluripotent stem cells (iPSCs). Also provided are methods for reprograming a non-naïve PSC into a TSI naïve iPSC by contacting the cell to be reprogrammed with effective amounts of compounds for a sufficient period of time to reprogram the cell into a TSI naïve iPSC.Type: ApplicationFiled: September 18, 2015Publication date: July 6, 2017Inventors: Hongkui DENG, Riguo FANG, Kang LIU, Weifeng YANG
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Publication number: 20170191039Abstract: The disclosure in some aspects relates to recombinant adeno-associated viruses having distinct tissue targeting capab- lities. In some aspects, the disclosure relates to gene transfer methods using the recombinant adeno-associate viruses. IN some as- pects, the disclosure relates to isolated AAV capsid proteins and isolated nucleic acids encoding the same.Type: ApplicationFiled: February 19, 2015Publication date: July 6, 2017Applicant: University of MassachusettsInventors: Guangping Gao, Li Zhong
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Publication number: 20170191040Abstract: The invention provides peptides derived from a ubiquitous protein, and nucleic acids encoding such peptides. The invention extends to various uses of these peptides and nucleic acids, for example, as antigens for use in vaccines per se and in the generation of antibodies for use in therapeutic drugs for the prevention, amelioration or treatment of infections caused by sepsis-inducing bacteria. The invention particularly benefits immunocompromised hosts such as neonates, babies, children, women of fertile age, pregnant women, foetuses, the elderly and diabetics.Type: ApplicationFiled: June 12, 2015Publication date: July 6, 2017Inventors: Paula Maria DAS NEVES FERREIRA DA SILVA, Pedro Jorge Fonseca MADUREIRA
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Publication number: 20170191041Abstract: The subject invention concerns materials and methods for treating or preventing disease and conditions associated with various sulfatase enzymes that are defective or that are not properly expressed in a person or animal. In one embodiment, the disease is Sanfilippo A (MPS-IIIA) disease. The subject invention also concerns materials and methods for treating or preventing multiple sulfatase deficiency (MSD) in a person or animal. Compounds of the invention include a fusion protein comprising i) a mammalian sulfatase, or an enzymatically active fragment or variant thereof, and ii) a plant lectin or a binding subunit thereof. In a specific embodiment, the mammalian sulfatase is a human sulfatase, or an enzymatically active fragment or variant thereof. Polynucleotides encoding the fusion proteins are also contemplated for the subject invention. The subject invention also concerns materials and methods for producing proteins of the invention.Type: ApplicationFiled: July 10, 2015Publication date: July 6, 2017Inventor: DAVID N. RADIN
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Publication number: 20170191042Abstract: The present disclosure relates to a new class of cytokinin biosynthetic enzymes, cytokinin synthases, which have two domains: an isopentenyl transfer (IPT)-like domain and a cytokinin nucleotide phosphoribohydrolase (PRH)-like domain. The invention provides compositions and methods for the recombinant production of cytokinin synthase, host cells and transformants that include the cytokinin synthases, as well as compositions and formulations that include the disclosed cytokinin synthase.Type: ApplicationFiled: March 8, 2017Publication date: July 6, 2017Applicant: Dow AgroSciences LLCInventors: William T. Beeson, IV, Patrick John Westfall
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Publication number: 20170191043Abstract: The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.Type: ApplicationFiled: March 3, 2017Publication date: July 6, 2017Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Paulina Elena Salim, Derek J. Smith, Jacob Janey, Birgit Kosjek, Weng Lin Tang, Helen Hsieh, Son Q. Pham
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Publication number: 20170191044Abstract: Disclosed are DNA polymerases having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of RT and polymerase inhibitors relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.Type: ApplicationFiled: March 17, 2017Publication date: July 6, 2017Inventors: Keith Bauer, Thomas W. Myers, Fred Reichert, Joseph San Filippo, Rachel Shahinian, Shawn Suko
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Publication number: 20170191045Abstract: The invention provides compositions and methods useful for the treatment and prevention of conditions associated with myocardial infarction.Type: ApplicationFiled: August 4, 2015Publication date: July 6, 2017Inventors: Maria Antonia BLASCO MARHUENDA, Bruno BERNARDES DE JESUS, Christian BAER, Rosa María SERRANO RUIZ, Fàtima BOSCH I TUBERT, Eduard AYUSO, Ivan FORMENTINI, Maria BOBADILLA, Jacques MIZRAHI
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Publication number: 20170191046Abstract: A lactone hydrolase having improved activity towards ?-zearalenol is disclosed. The lactone hydrolase has a modified amino acid sequence of SEQ ID NO: 5, wherein the modification is a substitution of valine at position 167 with histidine. A method of degrading ?-zearalenol using such lactone hydrolase is also disclosed.Type: ApplicationFiled: December 1, 2016Publication date: July 6, 2017Inventors: Rey-Ting Guo, Yingying Zheng, Zhongxia Xu, Chun-Chi Chen, Wei Peng, Weidong Liu, Je-Ruei Liu, Yanhe Ma
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Publication number: 20170191047Abstract: Provided herein are proteins having A-specific RNase activity. A protein having A-specific RNAse activity is referred to herein as a Csx1 protein. A Csx1 protein is an endoribonuclease, and has the activity of cleaving the phosphodiester bond in a single strand of a target RNA molecule on the 3? (downstream) side of an adenosine base to result in a first cleavage product having a 5? hydroxyl group and a second cleavage product having a 2?,3?-cyclic phosphate at the 3? end. Also provided herein are methods for using a Csx1 protein. In one embodiment, the method includes incubating a sample that includes an isolated Csx1 protein and a target RNA molecule under suitable conditions for cleavage of the target RNA molecule. Also provided is a genetically modified microbe that includes an exogenous polynucleotide including a nucleotide sequence encoding a Csx1 protein, and a method for making Cxsl protein.Type: ApplicationFiled: November 16, 2016Publication date: July 6, 2017Inventors: Rebecca M. Terns, Michael P. Terns, Nolan F. Sheppard
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Publication number: 20170191048Abstract: The disclosure provides a polypeptide with a mutation having cellobiohydrolase activity and improved thermal stability.Type: ApplicationFiled: May 26, 2015Publication date: July 6, 2017Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Kazuhiko ISHIKAWA, Seiichiro KISHISHITA, Makoto NAKABAYASHI, Saori KAMACHI, Toshiaki YANAMOTO, Tatsuya FUJII
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Publication number: 20170191049Abstract: The carbohydrate processing enzyme PNGase F was catalytically inactivated through mutation. Additional mutations yielded a catalytically inactive carbohydrate-binding protein with lectin-like properties including high affinity and specificity N-linked glycans, O-linked glycans, or both.Type: ApplicationFiled: April 17, 2015Publication date: July 6, 2017Applicants: UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC., GLYCOSENSORS AND DIAGNOSTICS, LLCInventors: Kausar N. Samli, Robert J. Woods, Loretta Yang
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Publication number: 20170191050Abstract: The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.Type: ApplicationFiled: February 13, 2017Publication date: July 6, 2017Inventors: Gjalt W. Huisman, Nicholas J. Agard, Benjamin Mijts, Jonathan Vroom, Xiyun Zhang
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Publication number: 20170191051Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are labeled polymerase conjugates including a polymerase linked to one or more labels, wherein the conjugate has polymerase activity. Such conjugates can exhibit enhanced biological activity and/or superior detectability as compared to conventional labeled polymerases. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.Type: ApplicationFiled: January 9, 2017Publication date: July 6, 2017Inventor: Theo NIKIFOROV
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Publication number: 20170191052Abstract: The present disclosure relates to the generation of an activated platelet product in which one or more of the presence or absence of clots, the timing of clot formation (if present), and/or the mechanical strength of clots (if present) is controlled by the presence or concentration of calcium ions during the activation process. In certain embodiments, the calcium ion concentration is controlled in the presence of pulsed electric fields or a chemical activator (e.g., thrombin) as part of the activation process.Type: ApplicationFiled: December 30, 2015Publication date: July 6, 2017Inventors: Vasile Bogdan Neculaes, Andrew Soliz Torres, Steve Lambert Klopman
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Publication number: 20170191053Abstract: Provided herein are methods and components for successive capture of nucleic acids using magnetic glass particles.Type: ApplicationFiled: January 4, 2017Publication date: July 6, 2017Inventors: Alan Casilla, Jennifer Griswold, Cherie Holcomb, Trevor Hryce, Karan Kampani, Sharon Legaspi