Abstract: Described is a dispensing needle for a fraction collector. The dispensing needle includes a conduit having a fluid channel to conduct a chromatographic flow, an interior wall that defines the fluid channel, an exterior surface and an endface through which the chromatographic flow is dispensed. The dispensing needle also includes a coating of a hydrocarbon material or a fluorocarbon material that is bonded to the endface. The coating is also bonded to at least a portion of the exterior surface that is adjacent to the endface and at least a portion of the interior wall that is adjacent to the endface. The coating operates to reduce a droplet volume of a liquid dispensed from the endface that may remain at the tip of the dispensing needle. Consequently, the concentration variation in a collected fraction due to a missing droplet or extra droplet is reduced.

Abstract: A system and a method is provided for calculating and assigning one or more indicative properties (e.g., cetane number, pour point, cloud point and/or aniline point) of a fractions of a crude oil sample based upon gas chromatographic simulated distillation data.

Abstract: An ion exchange chromatographic packing material is described that includes a copolymer grafted to support resin particles. The copolymer includes an ion exchange group, an ionic crosslinking group configured to ionically bind to the ion exchange group, and an adjustable ionization state group having at least a first net charge at the first pH and a second net charge at the second pH. An overall first net charge of the chromatographic packing material at the first pH is opposite in polarity to the overall second net charge of the chromatographic packing material. This allows impurities to be removed from the chromatographic packing material at the second pH.

Abstract: A method in which a concentration of a chemical species of interest is obtained. The method comprises measuring a property (e.g. the oxidation reduction potential) of a reagent (typically based on a simple single electron redox couple) to obtain a baseline measurement. The reagent is mixed with the solution under test, then the property of the mixture is measured to obtain a post reaction measurement. Then the concentration of the chemical species of interest is determined based on the baseline measurement and the first post reaction measurement, typically by calculating a difference of the baseline measurement and the post reaction measurement, then using the difference and a pre-determined conversion table to determine the concentration of the chemical species of interest.

Abstract: A gas concentration monitoring system (100) is provided. The system (100) includes a radiation source (104) having one or more emitting elements and a radiation sensor (106) having one or more sensing elements configured to detect radiation received at the radiation sensor (106). A reactive material (108) is located between the radiation source (104) and the radiation sensor (106) and is configured to react to the presence of a gas such as carbon dioxide, wherein the reaction of the reactive material (108) impacts an amount of radiation detected at the radiation sensor (106).

Abstract: Embodiments relate generally to systems and methods for calibrating a gas detector. Some embodiments may include a gas detector comprising one or more sensing elements operable to detect volatile organic compounds (VOC), and a probe operable to provide air flow into the gas detector, wherein the probe comprises: a housing operable to attach to a main body of the gas detector and operable to hold a calibration tube during calibration of the gas detector; a cap operable to attach to the housing and operable to fit over and cover the calibration tube; and one or more inlets, wherein when a calibration tube is inserted into the probe, the air flows through the calibration tube. In some embodiments of the gas detector, the calibration tube inserted into the probe comprises activated carbon fiber (ACF) filter material.

Abstract: A gas sensor 100 includes a sensor element 110 having a gas inlet 111; an inner protective cover 130 which has a sensor element chamber 124 thereinside and in which at least one element-chamber inlet 127 and at least one element-chamber outlet 138a are arranged; and an outer protective cover 140 in which at least one outer inlet 144a and at least one outer outlet 147a are arranged. A cross-sectional area ratio S1/S2, which is a ratio of a total cross-sectional area S1 [mm2] of the outer inlet 144a to a total cross-sectional area S2 [mm2] of the outer outlet 147a, is more than 2.0 and 5.0 or less.

Abstract: A gas sensor 100 includes a sensor element 110 having a gas inlet 111; an inner protective cover 130 which has a sensor element chamber 124 thereinside and in which at least one element-chamber inlet 127 and at least one element-chamber outlet are arranged; and an outer protective cover 140 in which at least one outer inlet 144a and at least one outer outlet are arranged. A minimum path length P from the outer inlet 144a to the gas inlet 111 is 5.0 mm or more and 11.0 mm or less.

Abstract: A gas sensor 100 includes a sensor element 110 having a gas inlet 111; an inner protective cover 130 which has a sensor element chamber 124 thereinside and in which an element-chamber inlet 127 and an element-chamber outlet 138a are arranged; and an outer protective cover 140 including a body portion 143, which has a cylindrical shape and in which an outer inlet 144a is arranged, and a front end portion 146, which has an inner diameter smaller than that of the body portion 143 and in which an outer outlet 147a is arranged. The outer inlet 144a includes a horizontal hole 144b arranged in a side portion 143a of the body portion 143 of the outer protective cover 140. The outer outlet 147a is not arranged in a side portion 146a of the front end portion 146 of the outer protective cover 140.

Abstract: An inner protective cover 130 of a gas sensor forms an element-chamber inlet 127 having a first outside opening 128a, a second outside opening 128b, and an element-side opening 129. The second outside opening 128b is disposed such that the path of a measurement-object gas from the first outside opening 128a to the element-side opening 129 of the element-chamber inlet 127 communicates in the middle thereof with a first gas chamber 122, and that there is a path shorter than the shortest path of the measurement-object gas extending from an outer inlet 144a through the first outside opening 128a to a gas inlet 111.

Abstract: Methods and sensors for detection and quantification of one or more analyte in a test sample are described. A response profile of a gas sensor to a control sample of a known interrogator gas is determined. The gas sensor is exposed to a test sample then to a second sample comprising the known interrogator gas, and a test sample response profile of the gas sensor is determined. One or more test sample sensor response profiles are compared with one or more control sensor response profiles for detecting, identifying, and quantifying one or more analytes in the test sample.

Abstract: A system includes a sensor configured to be disposed within a reservoir of a machine having moving parts that are lubricated by a liquid in the reservoir. The sensor is configured to obtain a measurement of the liquid that is representative of at least one of a quantity or quality of the liquid in the reservoir. The system also includes a device body operably coupled to the sensor. The device body has a processing unit that is operably coupled to the sensor and configured to generate first data signals representative of the measurement of the liquid. The device body also includes a transmitter that is configured to wirelessly communicate the first data signals to a remote reader.

Abstract: A system, method and computer program product are provided for calculating one or more indicative properties, e.g., one or more of the cetane number, octane number, pour point, cloud point and aniline point of oil fractions, from the density and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) of a sample of an oil sample.

Abstract: A system and a method are provided for calculating one or more indicative properties, e.g., one or more of the cetane number, octane number, pour point, cloud point and aniline point of oil fractions, from the density and high pressure liquid chromatography (HPLC) data of a sample of the crude oil.

Abstract: A method of obtaining a spectra on a hydrocarbon containing sample, that may include (A) Contacting the sample with an ionic liquid to create a prepared sample, and/or may also include (B) Processing the prepared sample through a spectroscopic system to obtain a spectra corresponding to the sample, wherein the spectra is suitable for determining the presence of and identity of any organic materials present sample. The sample may comprise soil, fluids, plants, drill cuttings, drilling mud, cores, water, blood, food, formation tester samples, or combinations thereof.

Abstract: New metrics are disclosed for characterizing polyethylene copolymers which can be computed from the Cross-Fractionation Chromatography data of these polymers. These metrics are able to quantify the Broad Orthogonal Composition Distribution (BOCD) character of the polymers, and they can be used to discriminate polymers with an enhanced BOCD character from polymers that have the BOCD character to a lesser extent or from polymers that have the conventional molecular weight distribution and/or branching distribution.

Abstract: According to one embodiment, a sensor includes a nonmagnetic layer and a plurality of magnetic field sensors. The nonmagnetic layer has a first surface and a second surface. The magnetic field sensors are arranged along the second surface. The second surface is between the first surface and the magnetic field sensors. Each of the magnetic field sensors includes a first magnetic layer, a second magnetic layer provided between the first magnetic layer and the nonmagnetic layer, and an intermediate layer provided between the first magnetic layer and the second magnetic layer. The intermediate layer is nonmagnetic. A distance between the first surface and the second magnetic layer is not more than a pitch of the magnetic field sensors.

Abstract: Provided herein are Si-based materials, methods of making the Si-based materials, and methods for using the Si-based materials. In embodiments, a silicon-based material comprises an aggregate of particles, the particles comprising an ordered array of nanostructures, the nanostructures comprising amorphous silicon, wherein at least some pairs of adjacent nanostructures are connected by one or more bridges comprising amorphous silicon, the one or more bridges extending from the surface of one nanostructure of the pair to the surface of the other nanostructure in the pair.

Abstract: A method of setting a handover position of a gripping device at a laboratory automation system is presented. A position of a position determining device held by the gripping device is detected using position sensors in order to determine the handover position. A laboratory automation system configured to perform such a method is also presented.

Abstract: Nanopore arrays are fabricated by controlled breakdown in solid-state membranes integrated within polydimethyl-siloxane (PDMS) microfluidic devices. This technique enables the scalable production of independently addressable nanopores. By confining the electric field within the microfluidic architecture, nanopore fabrication is precisely localized and electrical noise is significantly reduced during sensing.

Abstract: A method of controlling a blood analyzer for measuring platelets is provided. The method comprises: determining a relationship between at least one first measurement value obtained by detecting platelets in at least one previous test by an electrical type detector of the blood analyzer and at least one second measurement value obtained by detecting the platelets in the at least one previous test by an optical type detector of the blood analyzer, and controlling the blood analyzer to prepare the first and/or second measurement sample for a current test according to the determined relationship.

Abstract: A wet standard calibration system which utilizes a heat conducting foam with the liquid therein to serve as a primary heating device to avoid the need to include mechanical agitation. The system is particularly useful for generating a vapor comprising water and ethanol which can be used for the calibration of breath alcohol testers.

Abstract: Methods and compositions for drug discovery, analysis and treatment of a proliferative disorder characterized by abnormal cells in a mammalian subject are provided according to aspects of the present invention which include administering a pharmaceutically effective amount of a combination of: a cytotoxic agent, a SET agonist and a SET ribosome antagonist. Methods and compositions according aspect of the present invention incorporate agents effective to regulate and/or affect selective translation in a cell characterized by abnormal proliferation, such as a cancer cell, thereby promoting death of the cell.

Abstract: The present inventors show that cannibal cells can undergo senescence after entosis in vivo and that the tumor suppressive protein p53 act as a repressor of this phenomenon. They therefore propose new tools to study the molecular pathways involved in the cannibalism process, for example by measuring the expression levels of p53 or splice variants thereof (such as ?133TP53, TP53?, TP53? or ?40TP53), the release of extracellular ATP or purinergic P2Y2 receptor activity. The present inventors also demonstrated that the detection of senescent cannibal cells in breast adenocarcinoma obtained from patients treated with neo-adjuvant therapy positively correlates with good patient's response to treatment. Altogether, these results provide the first evidence that detection of cellular cannibalism and senescence simultaneously in tumors helps for the diagnosis of disease outcomes and for the prediction of treatment efficiency against cancer diseases.

Abstract: Methods of selecting test compounds on the basis of their cellular response profiles are disclosed. For a given test compound, a cellular response is determined by introducing into an array of individually addressable microwells a population of cells comprising a plurality of cell types and contacting the cells with the test compound. The cellular response profile for the test compound is then compared to a desired cellular response profile, and the test compound is selected based on the comparison.

Abstract: The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1 R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners. Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions.

Abstract: Disclosed herein are devices including a substrate, one or more regions of electrically active material, and optionally at least one electrode. Also disclosed herein are devices or systems including a substrate, one or more regions of electrically active material including muscle cells and neuronal cells, and optionally at least one electrode. In some embodiments, the muscle cells and neuronal cells form one or more neuromuscular junctions at defined locations on the electrically active material. Also disclosed are methods of using the devices and systems for analyzing the effect of compounds on neuronal cell, muscle cell and/or neuromuscular junction activity.

Abstract: A method for characterizing stem cell differentiation includes harvesting differentiation media supernatant containing secreted analytes from key time points during a stem cell differentiation, performing at least one of a qualitative and a quantitative analysis of the differentiation media supernatant with respect to at least one secreted analyte, and identifying trends in analyte expression based on at least one of the qualitative and quantitative analysis of the differentiation media supernatant.

Abstract: Provided are age-modified cells and method for making age modified cells by reducing or increasing the level of genomic nucleic acid methylation in the cells. The aging and/or maturation process can be accelerated or reduced and controlled for young, aged, mature and/or immature cells, such as a somatic cell, a stem cell, a stem cell-derived somatic cell, including an induced pluripotent stem cell-derived cell, by reducing or increasing the level of genomic nucleic acid methylation in the cells. Methods described by the present disclosure can produce age-appropriate cells from a somatic cell or a stem cell, such as an old cell, young cell, immature cell, and/or a mature cell. Such age-modified cells constitute model systems for the study of late-onset diseases and/or disorders.

Abstract: The invention relates to the surprising find that low density lipoprotein receptor-deficient mice (LDLr?/?) mice when fed with high energy diets produce controllable and consistent diabetic complications, especially renal damage, similar to the human pathophysiology and biological response. The invention thus comprises a method for discovering a preventive or therapeutic regimen for the prevention or treatment of diabetic micro- or macrovascular complications, comprising the steps of: a. feeding LDLr?/? mice, which have not been treated with streptozotocin, with a high energy diet; b. before, during and/or after this diet treating the mice with the preventive or therapeutic regimen; c. checking whether any change in the micro- or macrovascular system of the animal occurs. Specifically in such a method renal damage is assessed. Also use of said mice fed with a high energy diet for studying the diabetic micro- and macrovascular complications is part of the invention.

Abstract: The invention provides methods of detecting a natriuretic protein, a troponin, and galectin-3 in samples from subjects in order to determine risk of developing new onset heart failure with preserved ejection fraction (HFpEF).

Abstract: Devices and kits for detecting molecular targets involved in the onset of parturition are presented, as are methods of using the same. The devices and kits include one or more associators specific for molecular targets, such as microRNAs and proteins and peptide fragments thereof. The associators, such as aptamers or antibodies, bind their targets, when such targets are present in a fluid sample from a pregnant woman, to produce a detectable signal. The signal may be produced in a lateral flow device.

Abstract: This disclosure presents an Enzyme-Linked Immunosorbent Assay (ELISA) for the selective detection of anti-Mycoplasma hyorhinis IgG in porcine serum, which may contain antibodies specific to multiple other Mycoplasma spp.

Abstract: Autologous bone marrow cells (BMC) are transplanted to a heterologous site in a patient after a sample of the patient's BMC has been tested and found to have a phenotypic profile which meets minimum criteria for transplantation. The phenotypic profile may be obtained by screening a sample of bone marrow cells (BMC) from the patient for the phenotypic profile, such as a CD profile, the phenotype profile may be assessed to determine the likelihood that the BMC will be suitable for transplantation to the heterologous tissue site without enriching particular phenotypic population(s) of the BMC.

Abstract: The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

Abstract: The present invention provides methods for evaluating the quality of a preparation of antigen presenting cells, such as dendritic cells. Assays for antigen-independent co-stimulation of T cells and for presentation of predetermined antigen by APCs are provided.

Abstract: Methods for identifying and isolating CD8 T cells that produce interleukin-13 upon activation are provided. The present methods leverage one or more newly-identified biomarkers to identify such CD8 T cells and, in certain cases, sort the same. Certain methods comprise obtaining a sample from a mammal, quantifying a level of expression of one or more biomarkers therein, and determining if the level of expression is elevated as compared, wherein an elevated expression level is indicative of an active disease state. Antisera and antibodies are also provided. In particular, an anti-C10orf128 antiserum formulated against a particular peptide is provided, such anti-C10orf128 antiserum characterized in that it identifies a subset of CD8 T cells that produce interleukin-13 upon activation.

Abstract: A reagent kit used for detecting lipoprotein-associated phospholipase A2, and a preparation method for the reagent kit. The reagent kit comprises one or a plurality of first anti-lipoprotein-associated phospholipase A2 antibodies used for binding lipoprotein-associated phospholipase A2 to be measured, and one or a plurality of second anti-lipoprotein-associated phospholipase A2 antibodies marked with a trace marker and binding with the lipoprotein-associated phospholipase A2 to be measured at another site, different from the binding site of the lipoprotein-associated phospholipase A2 to be measured and the first anti-lipoprotein-associated phospholipase A2 antibodies. The reagent kit also comprises a displacing agent, so as to further increase the detection accuracy of the reagent kit.

Abstract: The invention relates to a prenatal diagnostic method for the determination of a fetal chromosomal aneuploidy in a biological sample obtained from a pregnant woman, which method comprises enrichment and quantification of selected cell-free deoxyribonucleic acid sequences showing consensus nucleosome binding regions.

Abstract: Provided herein are predictive biomarker signatures that identify patients as likely to benefit from TIL therapy. Also provided are tumor immunotherapy resistance pathways that may be targets of combination therapies to enhance TIL therapy.

Abstract: Disclosed herein are methods for treating subjects with breast cancer, comprising determining a therapeutic regimen for cancer by measuring the level (amount) of proteins of one or more biomarkers. Also disclosed are methods of predicting or assessing therapeutic outcome for subject.

Abstract: Cancer patients make antibodies to tumor-derived proteins that are potential biomarkers for early detection. Twenty-eight antigens have been identified as potential biomarkers for the early detection of basal-like breast cancer (Tables 1, 2). Also, a 13-AAb classifier has been developed that differentiate patients with BLBC from healthy controls with 33% sensitivity at 98% specificity (Table 3).

Abstract: Provided herein are kits for detecting and quantifying the level of a metastatic cancer biomarker(s) in a patient. The kits may contain a detection and quantifying means, such as, biomarker specific antibodies. Also provided are methods for determining a prognosis, a stratification and/or monitoring of a metastatic cancer in a patient. The level or activity of at least two biomarkers, such as obscurin, PI3K, E-cadherin, or N-cadherin is measured in a patient sample and compared to corresponding biomarkers in a control sample. A significant difference in the level or activity of the biomarkers between the patient sample and control is indicative of a metastatic cancer. In addition a method for treating a metastatic breast cancer via administration of a PI3K inhibitor and an agent that increases expression of obscurin is provided.

Abstract: The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations.

Abstract: This document features methods of evaluating mammals by assessing expression of B7-H1 in a body fluid.

Abstract: Provided herein are methods of treating a nonhematopoietic malignant tumor in a patient and methods of prognosing a nonhematopoietic malignant tumor in a patient, comprising administering to the patient a therapeutically effective amount of an agent that preferentially kills or inhibits proliferation or activity of leukocytes relative to nonhematopoietic cells.

Abstract: An object of the present invention is to provide a fluorescent probe that easily penetrates a cell and is capable of selectively and efficiently detecting singlet oxygen generated in the cell, a singlet oxygen detection agent containing the fluorescent probe, and a singlet oxygen detection method using the fluorescent probe. The fluorescent probe for detecting singlet oxygen comprises a compound having a silicon-rhodamine skeleton and a skeleton that increases the fluorescence of the fluorescent probe after trapping singlet oxygen to be greater than the fluorescence before trapping singlet oxygen, or a salt, hydrate, or solvate thereof.

Abstract: The present application relates to a method for determining a risk of preterm birth and/or low birth weight of a pregnant female comprising measuring a galectin-3 (Gal-3) level in a blood, plasma, or serum sample obtained from the pregnant female, or a kit or marker which may be used in the method. The present application also relates to a medicament effective to reduce a risk of preterm birth and/or low birth weight, a pharmaceutical composition comprising the medicament, or a method for reducing a risk of preterm birth and/or low birth weight of a pregnant female comprising administering the medicament or the pharmaceutical composition to the pregnant female.

Abstract: A method for analyzing a sample includes trapping a first ion packet in a first segment of a multi-segment reaction cell; trapping a second ion packet in a second segment of the multi-segment reaction cell; and trapping a third ion packet in a third segment of the multi-segment reaction cell. At least one of the first, second, and third ion packets includes precursor ions, and at least another one of the first, second, and third ion packets includes reagent ions. The method further includes mixing the first, second, and third ion packets within the reaction cell to cause a reaction between the precursor ions and the reagent ions to form product ions.

Abstract: The invention provides populations of isolated peptides useful for the detection of antibodies that bind to Anaplasma antigens. The peptide populations comprise peptides derived from immunogenic fragments of the Anaplasma Outer Membrane Protein proteins. The invention also provides devices, methods, and kits comprising the populations of isolated peptides useful for the detection of antibodies that bind to Anaplasma antigens and the diagnosis of anaplasmosis. Methods of identifying the particular Anaplasma species infecting a subject using the peptide populations of the invention are also disclosed.