Abstract: The present invention relates to compositions of benefit agent containing delivery particles and slurries comprising benefit agent containing delivery particles, compositions comprising said particles, and processes for making and using the aforementioned particles and compositions. Such slurries and benefit agent containing delivery particles are stable and when employed in compositions, for example, cleaning or fabric care compositions, the particles in such slurries increase the efficiency of benefit agent delivery, thereby allowing reduced amounts of benefit agents to be employed. In addition to allowing the amount of benefit agent to be reduced, such particles allow a broad range of benefit agents to be employed. The compositions can comprise particles, cakes, powders, particulates, aggregates, aggregate clusters and slurries comprising the benefit agent containing delivery particles.

Abstract: Disclosed herein are compositions for removing silicone resins and methods of thinning a substrate by using the same, as well as related methods, apparatus and systems for facilitating the removal of silicone resins. More particularly, disclosed herein are compositions for removing silicone resins, the compositions including a heterocyclic solvent and an alkyl ammonium fluoride salt represented by a formula, (R)4N+F?, wherein R is a C1 to C4 linear alkyl group. Silicone resins may be effectively removed by using the compositions since the compositions exhibit an excellent decomposition rate with respect to the silicone resins that remain on a semiconductor substrate in a process of backside grinding of the semiconductor substrate, backside electrode formation, or the like.

Abstract: Methods comprising the steps of contacting a surface with a liquid composition comprising a cleaning agent, an adhesion promoter, and at least one dye, the surface having a first surface contamination; transitioning at least one portion of the surface in the first surface contamination state to a second surface contamination state and simultaneously distributing the at least one dye and at least one adhesion promoter on the surface associated with the second surface contamination state, the second surface contamination state having reduced or eliminated surface contamination, the transitioning step simultaneously cleaning and preparing the at least one portion of the surface for adhesion to another material; and identifying remaining surface in the first surface contamination state, and compositions adapted for the method thereof.

Abstract: What is disclosed is a liquid composition containing: 80 mol % to 99.9999 mol % of (Z)-1,2-dichloro-3,3,3-trifluoropropene (1223Z) and 0.0001 mol % to 20 mol % of (E)-1,2-dichloro-3,3,3-trifluoropropene (1223E). The liquid composition has small impact on the global environment and exerts azeotrope-like properties. Therefore, the composition of the liquid composition is not substantially changed even when the liquid composition is used in an open system or used for a long period. The composition of the liquid composition is also rarely changed even when the liquid composition is recovered by distillation. Therefore, the liquid mixture according to the present invention can be used suitably as a cleaning agent (a solvent).

Abstract: A method for formulating a composition contains the step of coating a granular absorbent material with a coating agent to produce a coated absorbent material and mixing the coated absorbent material with a sanitation agent, wherein the coated absorbent material absorbs the sanitation agent to form the liquid loading composition. Liquid-loading compositions that can absorb liquids in many applications, including clean-up and farming, are also disclosed.

Abstract: Methods relating to making product compositions that include encapsulates and borate compounds, where the encapsulates include polyvinyl alcohol polymer. Compositions made from such methods. Encapsulate slurries.

Abstract: Methods relating to making product compositions that include encapsulates, borate compounds, and a cationic polysaccharide, where the encapsulates include polyvinyl alcohol polymer. Compositions made from such methods. Encapsulate slurries.

Abstract: A liquid laundry detergent composition capable of suspending particles and insoluble materials while remaining readily pourable. The composition is stable over long periods of time. In one aspect, the liquid laundry detergent comprises in an aqueous medium: a) at least one nonethoxylated anionic surfactant; b) at least one ethoxylated anionic surfactant; c) at least one nonionic fatty alcohol ethoxylate surfactant; d) an optional surfactant selected from a nonionic surfactant other than c), a cationic surfactant, an ampholytic surfactant, and mixtures thereof; and e) a nonionic, amphiphilic, emulsion polymer.

Abstract: Methods relating to making product compositions that include encapsulates and borate compounds, where the encapsulates include polyvinyl alcohol polymer. Compositions made from such methods.

Abstract: A consumer product composition comprises a consumer product adjunct ingredient, a microcapsule, and deposition polymer disposed on an outer surface of the microcapsule. The deposition polymer has a Water Uptake Value (WUV) of at least about 2 grams per gram, as determined according to the WATER UPTAKE VALUE TEST METHOD. The microcapsule comprises a shell material encapsulating a core material, wherein the shell material comprises a polyacrylate and the core material comprises a benefit agent.

Abstract: The present disclosure relates to agents and methods for reducing calcium in beverages, in particular alcoholic and fermented beverages such as wine, beer, lager, ale, cider and perry. The agent comprises a pectin in a powder form or a salt thereof.

Abstract: A kit for separating adipose tissue-derived stromal vascular fraction is easy to operate so that even a non-specialist can use the kit and can perform washing and suspending processes for stromal vascular fraction in one tube repeatedly at the same time, thereby minimizing exposure to air, resulting in a low risk of secondary infection caused by external agents. Accordingly, a survival rate and a recovery rate of the stromal vascular fraction with respect to the amount of adipose tissue are increased whereby less adipose tissue is required and thus the stromal vascular fraction is efficiently and easily separated from the adipose tissue.

Abstract: A system for growing and reproducing microorganisms that includes a basin system that includes a number of basins, where each basin has a vertical meandering system which is formed by partitions and which can be illuminated, each basin is filled with a nutrient suspension, at least one outer wall of each basin is double-walled such that a cavity is formed, a temperature control medium for controlling the temperature of the nutrient suspension can flow through the cavity.

Abstract: An integrated bioreactor and reseeding chamber system and a separate bioreactor and complimentary reseeding chamber system comprising a bioreactor and a separate reseeding chamber. The bioreactor comprises a bioreactor chamber; a first groove attached to the bioreactor chamber; a second groove removably attached to the bioreactor chamber. A first bar and a second bar may be removably inserted into the first groove and the second groove respectively. Each of the first bar and the second bar may have at least an oval hole. At least a crossbar is attached substantially perpendicularly to the first bar and the second bar to form a crossbar-bars construct through the oval holes. A knob may be installed on each of the crossbars. The reseeding chamber may comprise dividers fixedly attached to a bottom of the reseeding chamber.

Abstract: Provided is a cell treatment apparatus that includes: an isolator that has an inner space maintained in aseptic conditions and is configured to treat cells in the inner space; trays and that are each configured to house plural kinds of articles, which include reagent containers with articles and reagents therein for use in treatment of cells in the inner space of the isolator, while positioning them; and pass boxes that are configured to respectively carry the trays with the plural kinds of articles housed therein into the isolator.

Abstract: According to various aspects and embodiments, an integrated sensor system is provided. In one example, such an integrated sensor system includes at least one unit cell, an analysis device, and a controller. The at least one unit cell may include an activation chamber containing an activation fluid, a biological chamber containing a biological component, a first membrane disposed in between the activation chamber and the biological chamber, an assay chamber configured to receive a sample, and a second membrane disposed in between the assay chamber and the biological chamber.

Abstract: A cassette comprising: a first reservoir arranged to couple to a first actuator for imparting flow to a cell-containing solution in a first direction through a filter; a second reservoir arranged to couple to a second actuator for imparting flow to a cell-containing solution in a second direction through the filter; the filter being in fluid communication with the first and second reservoirs, and when connected to a filtration unit is in further fluid communication to a sample inlet, and a sample outlet; wherein, the cassette is disposable and housed by a connector being removeably adjoinable to the filtration unit, the filtration unit comprising the first and second actuators and one or more valves.

Abstract: New strains of microalgae belonging to the Nitzschia genus allow high-yield production of lipids such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), and/or carotenoids such as fucoxanthin. Biomass containing the microalgae can be charged with fucoxanthin representing more than 0.2% by weight of the dry matter.

Abstract: The present disclosure provides a new mutant of Bacillus thuringiensis which is deposited under Accession number DSM 32419. The present disclosure also provides a method for manufacturing a growth promoter for promoting microalgal cell growth, including: (a) inoculating a mutant of Bacillus thuringiensis into a culturing medium to obtain a bacterial suspension, wherein the mutant of Bacillus thuringiensis is deposited under Accession number DSM 32419; (b) culturing the mutant of Bacillus thuringiensis in the bacterial suspension at least to a stationary phase to obtain a cultured medium of the mutant of Bacillus thuringiensis; and (c) performing a vacuum heating procedure on the cultured medium of the mutant of Bacillus thuringiensis to obtain the growth promoter for promoting microalgal cell growth, wherein the growth promoter for promoting microalgal cell growth contains active substances for promoting microalgal cell growth.

Abstract: A new method of using fungus to assist the growth of algae.

Abstract: The present invention provides a base material for cell culture comprising a polyester resin comprising a dicarboxylic acid unit and a diol unit, wherein 1 to 80% by mol of the diol unit is a diol unit having a cyclic acetal structure.

Abstract: Disclosed herein are improved methods for fabricating bioprinted, three-dimensional, biological tissues. The methods relate to exposures to low temperatures, incubations at low temperatures of various durations, and fabrication in environments without structural cross-linking treatments.

Abstract: A method of making a porous three-dimensional graphene mesh includes combining a graphene-containing material and a polymer having a plurality of hydroxyl groups in an alcohol solvent to form a mixture, adding a salt to the mixture, heating the mixture to form a gel, and washing the gel with water to remove the salt from the gel, leaving behind stable pores to form a scaffold. A three-dimensional porous graphene mesh includes a graphene-containing material and a polymer. The polymer is crosslinked with the graphene-containing material such that the Young's Modulus of the mesh is at least about 5 GPa.

Abstract: The invention relates to methods for generating multipotent mammary stem cells from isolated and cultured human breast luminal cells. The method comprises the steps: 1. isolating and growing normal differentiated cells in vitro; 2. treating the differentiated cells with either a conditioned medium from active fibroblasts or cytokine. The invention also relates to multipotent mammary stem cells, cultures of the multipotent stern cells, differentiated cells, tissues, organs derived from the culture multipotent stem cells isolated by the methods disclosed and therapeutic and other uses for those cells thereof.

Abstract: The present invention is directed to methods for the growth, expansion and differentiation of pluripotent stem cells on micro-carriers.

Abstract: The invention relates to a culture medium comprising an inhibitor of the BMP signaling pathway; and an inhibitor of the TGF/activin/nodal signaling pathway and to a method for obtaining a population of neural precursors using said culture medium.

Abstract: Chimeric transmembrane immunoreceptors (CAR) which include an extracellular domain that includes IL-13 or a variant thereof that binds interleukin-13R?2 (IL13R?2), a transmembrane region, a costimulatory domain and an intracellular signaling domain are described.

Abstract: Disclosed herein is a genetically-modified cell comprising in its genome a modified human T cell receptor alpha constant region gene, wherein the cell has reduced cell-surface expression of the endogenous T cell receptor. The present disclosure further relates to methods for producing such a genetically-modified cell, and to methods of using such a cell for treating a disease in a subject.

Abstract: A nucleic acid construct and an immune cell, which harbor nucleic acids encoding a CAR and nucleic acids encoding at least one anti-inflammatory or immunosuppressant protein and methods of using the same in treatment or amelioration of inflammation or immune-mediated autoimmunity are described.

Abstract: Compositions comprising synthetic membrane-receiver complexes, methods of generating synthetic membrane-receiver complexes, and methods of treating or preventing diseases, disorders or conditions therewith.

Abstract: The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and/or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and/or single-stranded run-on mRNAs.

Abstract: The present invention relates to a method for stabilizing an enzyme by storing the enzyme in the presence of a stable coenzyme. The present invention further relates to an enzyme stabilized with a stable coenzyme, and to the use thereof in test elements for detecting analytes.

Abstract: The present invention relates to provision of novel artificial bioluminescent enzymes. According to one embodiment of the present invention, there is provided a polypeptide, including any one of amino acid sequences (i) to (iii) below, and having a copepod luciferase activity: (i) an amino acid sequence represented by SEQ ID NO: 1 or 12; (ii) an amino acid sequence represented by SEQ ID NO: 1 or 12 in which one or several amino acids are substituted, added, or deleted; or (iii) an amino acid sequence having an identity of not less than 90% with an amino acid sequence represented by SEQ ID NO: 1 or 12.

Abstract: Transglutaminase variants capable of conjugating an antibody that is not conjugated by wild-type transglutaminase from Streptomyces mobaraensis.

Abstract: An isolated and/or purified ?-glucanotransferase from Exiguobacterium Acetylicum, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the ?-glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the ?-glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.

Abstract: The present disclosure relates to recombinant yeast strains capable of maintaining their robustness at high temperature as well as recombinant proteins expressed therefrom. The present disclosure also provides methods for using the recombinant yeast strain for making a fermentation product. The present disclosure further process a process for making recombinant yeast strains capable of maintaining their robustness at high temperature.

Abstract: Provided herein is an ?-fucosidase that can cleave a conjugate comprising an N-glycan and a label where the label is added by amine reactive chemistry. The ?-fucosidase also has an accelerated reaction time using Schiff base labeled N-glycans compared with bovine kidney fucosidase. A reaction mix, enzyme mix and kit comprising the ?-fucosidase are provided, as well as a method for analyzing glycoproteins. The ?-fucosidase finds particular use in analyzing the N-glycans of therapeutic glycoproteins.

Abstract: The present invention relates to protease variants and methods for obtaining protease variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: It is necessary to establish a purification method effective for the removal of endotoxin, in order to obtain thermolysin containing a reduced amount of contaminant endotoxin. On the premise of the establishment, accurate measurement of the amount of endotoxin that contaminates thermolysin is required. The present invention addresses the problem of providing a novel measurement method which enables accurate measurement of the amount of endotoxin that contaminates thermolysin. The present invention also addresses the problem of providing thermolysin containing a reduced amount of contaminant thermolysin, and the use thereof. There is provided thermolysin containing contaminant endotoxin in an amount of 1 EU/mg or less. There is also provided a method for measuring endotoxin that contaminates thermolysin, the method including pretreatment of deactivating the thermolysin.

Abstract: The present invention relates to a novel recombinant batroxobin mixture, a hemostatic composition comprising the same, and a method for preparing the same. According to the present invention, the hemostatic composition of the present invention has an excellent hemostatic effect by suppressing rebleeding, and maintains activity thereof even when prepared in a solid form, and thus, the composition of the present invention can be easily used by being applied as a topical hemostatic agent.

Abstract: Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly.

Abstract: Methods of modifying a dystrophin gene are disclosed, for restoring dystrophin expression within a cell having an endogenous frameshift mutation within the dystrophin gene. The methods comprising introducing a first cut within an exon of the dystrophin gene creating a first exon end, wherein said first cut is located upstream of the endogenous frameshift mutation; and introducing a second cut within an exon of the dystrophin gene creating a second exon end, wherein said second cut is located downstream of the frameshift mutation. Upon joining/ligation of said first and second exon ends dystrophin expression is restored, as the correct reading frame is restored. Reagents and uses of the method are also disclosed, for example to treat a subject suffering from muscular dystrophy.

Abstract: A method comprises: sorbing a sample solution comprising nucleic acids to a sample receiving portion of a quartz fiber filter by contacting the sample solution with the sample receiving portion; and washing the sample receiving portion while keeping most of nucleic acids around the sample receiving portion by flowing a wash solution through the sample receiving portion under a wicking force directed away from the sample receiving portion. An associated apparatus is also provided.

Abstract: The present invention relates to compositions and methods for cancer diagnostics, including but not limited to, cancer markers. In particular, the present invention provides methods and compositions for phage microarray profiling of cancer (e.g., prostate, lung, or breast cancer). The present invention further provides novel markers useful for the diagnosis, characterization, and treatment of cancers.

Abstract: The object of the invention is a method of synthesis and purification of a nucleoside and/or a nucleotide being a mono-, di- or triphosphate, wherein a Huisgen's azide-alkyne cycloaddition reaction is being performed using a compound of structure (1) with a compound chosen from the group of structures (2) to (5). The object of the invention is also a modified nucleoside and/or nucleotide of structure (10), a DNA molecule and an oligonucleotide library comprising one or more modified nucleotides and the use of said oligonucleotide library for aptamers selection.

Abstract: Aspects of the disclosure relate to barcoded chimeric adeno-associated virus (AAV) capsid libraries, chimeric capsids and related recombinant AAVs (rAAVs) identified using the libraries. Spedfically, the chimeric AAV capsid libraries comprise a plurality of nucleic adds encoding AAV capsid proteins, wherein each nucleic acid (i) encodes a unique AAV capsid protein having distinct polypeptide regions of greater than six amino acids in length that are derived from at least two different AAV serotypes, and (ii) comprises a unique barcode sequence. Further disdosed are methods of preparing an AAV library and identifying AAV capsids tropic for a target tissue.

Abstract: Described herein are methods and compositions for rapid and precise genome editing at any desired genomic position in almost all cells and organisms. The methods utilize a cytosine-specific Cas9 endonuclease and are suitable for applications requiring targeting cytosine adjacent DNA. The methods generally include introducing a cytosine-specific Cas9 endonuclease, a single guide RNA, and optionally a DNA repair template that is utilized in either non-homologous end joining or homology directed repair. The methods can be used to effect gene knockout, gene knockdown, gene substitution, or gene introduction in cells and organisms.

Abstract: The invention relates to isolated nucleic acids and rAAV-based compositions, methods and kits useful for treating genetic diseases (e.g., alpha-1 antitrypsin deficiency).

Abstract: The present invention provides an oligomer which allows exon 45 skipping in the human dystrophin gene.

Abstract: Double-stranded nucleic acid molecules (100, 200, 300, 400, 500 and 600) are provided comprising a sense strand (110, 210, 310, 410, 510 and 610) and an antisense strand (120, 220, 320, 420, 520 and 620). The sense strand has a 3?-overhang (115, 215, 315, 415, 515 and 615) of from four to eight nucleotides. The double-stranded nucleic acid molecules include double-stranded RNA (dsRNA), double-stranded DNA (dsDNA), double-stranded DNA-RNA (sense-antisense) hybrid and double-stranded RNA-DNA (sense-antisense) hybrid. The double-stranded nucleic acid molecules have beneficial properties in terms of unaided cellular delivery (e.g. in the absence of a cationic agent), cellular delivery and release of the antisense strand in a cell as a single-stranded molecule and the delivery of the antisense strand into the nucleus of a cell as a single-stranded molecule. Related compositions, cells, uses and methods are also provided.