Abstract: A lubricant base oil composed of an ester. The ester includes a component (A) derived from trimethylolpropane in a molar percentage Amol% of 25 to 42 mol %; components (B) derived from monovalent straight-chain saturated fatty acids each having a carbon number of 8 to 12 in a molar percentage Bmol% of 33 to 55 mol %: and a component (C) derived from adipic acid in a molar ratio Cmol% of 12 to 34 mol %. The components (B) include lauric acid in a molar percentage of 5 to 50 mol %, and (BCOOH+CCOOH)/AOH is 0.90 to 1.02. AOH represents a hydroxyl equivalent of the component (A), BCOOH represents a carboxyl group equivalent of the components (B); and CCOOH represents a carboxyl group equivalent of the component (C).

Abstract: A lubricating composition comprises a major amount of an oil of lubricating viscosity and 0.01 to 25 percent by weight based on the weight of the composition, of a poly(2-oxazoline) polymer. The polymer has the repeat unit: —N(COR1)CH2CH2— wherein the number of repeat units (n) in the polymer is an integer between 4 and 1000, such as between 4 and 500. The polymer carries an inorganic or organic nucleophilic polymerisation terminating group (t), and an initiator group (i) connected to the N atom of a repeat unit, the initiator group (i) being effective to initiate the polymerisation of linear, branched or cyclic hydrocarbyl moieties. At least 5% of the total number of the groups R1 in the polymer comprise alkenyl groups having between 15 and 20 carbon atoms.

Abstract: A lubricating composition comprises at least 50 percent by mass, based on the mass of the composition of an oil of lubricating viscosity and 0.01 to 25 percent by mass, based on the mass of the composition of an oil-soluble poly(2-oxazine) polymer. The polymer has the repeat unit: where the number (n) of repeat units is an integer between 4 and 500, such as between 4 and 400. The polymer carries an inorganic or organic nucleophilic polymerisation terminating group (t), and an initiator group (i) connected to the N atom of a repeat unit, the initiator group (i) being effective to initiate the polymerisation of linear, branched or cyclic hydrocarbyl moieties. R1 comprises a single or a mixture of linear, branched or cyclic hydrocarbyl groups having 1-50 carbon atoms, some or all having 12-50 carbon atoms, or of at least one macro-monomeric hydrocarbyl group with more than 50 carbon atoms.

Abstract: A lubricating composition comprises a major amount of an oil of lubricating viscosity and 0.01 to 25 percent by mass, based on the mass of the composition of an oil-soluble copolymer, The copolymer comprises units (a) and units (b): —N(COR1)CH2CH2—??(a) —N(COR2)CH2CH2CH2—??(b) wherein the polymer carries an inorganic or organic nucleophilic polymerisation terminating group (t), and an initiator group (i) connected to the N atom of a repeat unit (a) or (b), the initiator group (i) being effective to initiate the polymerisation of linear branched or cyclic hydrocarbyl moieties. R1 and R2 are the same or different and comprise a single or a mixture of linear, branched or cyclic hydrocarbyl groups having 1-50 carbon atoms, some or all having 12-50 carbon atoms, or of at least one macro-monomeric hydrocarbyl group with more than 50 carbon atoms.

Abstract: The present invention relates to a functional fluid composition comprising a glycol as a base material and a diamine-based noise reducer. According to the present invention as such, the functional fluid composition comprising a glycol as a base material and a diamine-based noise reducer represented by chemical formula 1 has an excellent noise reducing effect. In addition, the functional fluid composition of the present invention has an excellent metal corrosion inhibiting effect even, by using a diamine-based compound as a noise reducer, when a metal corrosion inhibitor composed of a triazole-based compound without including an amine-based compound is used as a metal corrosion inhibitor.

Abstract: The present invention relates to a functional fluid composition comprising a glycol as a base material and a monoamine-based noise reducer. According to the present invention as such, the functional fluid composition comprising a glycol as a base material and a monoamine-based noise reducer represented by chemical formula 1 has an excellent noise reducing effect. In addition, the functional fluid composition of the present invention has an excellent metal corrosion inhibiting effect even, by using a monoamine-based compound as a noise reducer, when a metal corrosion inhibitor composed of a triazole-based compound without including an amine-based compound is used as a metal corrosion inhibitor.

Abstract: The present invention provides a refrigerating machine oil including a lubricating base oil and a compound represented by the following formula (A): wherein Ra and Rb each independently represent a monovalent hydrocarbon group, Rc represents a divalent hydrocarbon group, X represents a polar group, and Za and Zb each independently represent oxygen atom or sulfur atom.

Abstract: Fabric softener compositions that include a quaternary ammonium ester fabric softening active, dispersed perfume, and a pentaerythritol ester, the compositions exhibiting viscosity stability while also delivering the softening benefits that are desired by consumers. Methods of making same.

Abstract: The present invention relates to a hand dishwashing detergent composition including a surfactant system and at least one triblock co-polymer of Formula (I): (EO)x-(PO)y-(EO)x wherein each x is independently on average between about 5 and about 50.

Abstract: The present invention relates to a hand dishwashing detergent composition including a surfactant system and at least one triblock co-polymer of Formula (I): (EO)x-(PO)y-(EO)x wherein each x is independently on average between about 1 and about 40, and y is on average between about 1 and about 15.

Abstract: The present invention relates to a hand dishwashing detergent composition including a surfactant system; at least one triblock co-polymer of Formula (I); and an amphiphilic alkoxylated polyalkyleneimine.

Abstract: A dishwasher composition includes at least one strong chelant, at least one weak chelant, a structural constituent, at least one surfactant, and a primary polymer. The primary polymer includes a copolymer having sulfonic acid monomer units and monomer units comprising one or more supplemental monomers, wherein the primary polymer comprises from 10 wt. % to 50 wt. % sulfonic acid monomer units and has a weight average molecular weight of less than or equal to 20,000 g/mol. The dishwasher composition can be a liquid or a gel and has a pH of from 7.0 to 9.5. Methods of washing articles include preparing a wash solution that includes water and the dishwasher composition, and contacting at least one article with the washing solution during at least a portion of a wash cycle of a commercially-available dishwasher.

Abstract: The present invention relates to compositions, methods and use of a mixture of enzymes having DNase activity and an enzyme having hexosaminidase activity.

Abstract: Provided is an alkaline protease mutant with improved stability to a chelating agent. An alkaline protease mutant, in which an amino acid residue at a position corresponding to position 294 of the amino acid sequence as shown in SEQ ID NO: 2 is substituted in the amino acid sequence as shown in SEQ ID NO: 2, or an amino acid sequence having an identity of at least 95% thereto.

Abstract: A solid cleaning composition, the composition including: one or more anionic surfactants, wherein the one or more anionic surfactants constitute 40 wt. % to 90 wt. % of the composition; a cationic biocide, wherein the cationic biocide constitute 0.0001 wt. % to 2 wt. % of the composition; and one or more humectants; wherein the one or more humectants constitute 0.03 wt. % to 35 wt. % of the compositions, the cationic biocide retaining its biocidal activity as demonstrated by a reduction of at least 1.0 log 10 in a standard hand wash test.

Abstract: This invention relates to a bleaching composition comprising hypochlorite-based bleach particles and moisture-sensitive particles encapsulating an oil within starch or starch-derivative matrix, the composition being surprisingly stable toward degradation of the starch upon storage.

Abstract: A semiconductor cleaning solution for cleaning a surface of a semiconductor device, and a method of use and a method of manufacture of the cleaning solution are disclosed. In an embodiment, a material is polished away from a first surface of the semiconductor device and the first surface is cleaned with the cleaning solution. The cleaning solution may include a host having at least one ring. The host may have a hydrophilic exterior and a hydrophobic interior.

Abstract: Liquid detergent compositions can be stably structured using amides of an aliphatic polyamine with two, three or four molecules of fully saturated hydroxyl alkyl acids, even in the presence of hydrolysing detergent ingredients such as lipase enzyme.

Abstract: The present invention relates to the use of a yeast protein extract to stabilise the haze or cloudiness of a drink, particularly beer and preferably white beer.

Abstract: A tank receives, mixes, and dispenses input materials including algae feed stock, a micro-nutrients, cleaning solution, a sterile gas, a heating/cooling fluid, carbon dioxide, and raw water with a water treatment system. A plurality of output stations include a vent to atmosphere, a heating/cooling media return, a cleaning disposal, and an algae concentrate/product. The vent to atmosphere is coupled to the tank. The heating/cooling media return is coupled to the heating/cooling media supply through the tank. The cleaning solution disposal station is coupled to the tank with a pump followed by a nano bubbler and an algae growing system. An algae concentrate/product station is coupled to the tank. An algae dewatering system is intermediate the tank and the algae concentrate/product. A water return line couples the algae dewatering system and the water treatment system.

Abstract: A layered, microfluidic array is disclosed. The array has a first layer with a culture channels extending in a first longitudinal direction. Each culture channel includes multiple traps that entrap cell or tissue samples. The array also has a second layer with microfluidic channels extending in a second longitudinal direction that is orthogonal the first longitudinal direction. A third layer, disposed between the first layer and the second layer, has pores arranged within the third layer such that each nest is vertically stacked above, and fluidly connected with, a corresponding culture chamber in the first layer. Each nest is fluidly isolated from adjacent nests by a fluid impermeable region of the third layer such that horizontal diffusion of water within the third layer is prevented.

Abstract: A vessel manufacturing method includes placing a bag-shaped, film-based vessel on a placement stage with concave portions formed on the stage, introducing fluid into the vessel placed on the stage, and pressing the vessel which is placed on the stage, by a pressing member while heating at least one of the stage and the pressing member. Meanwhile, a cell culture vessel includes a first vessel wall as a bottom wall and a second vessel wall. The first vessel wall is formed of a flat film having gas permeability. The second vessel wall is disposed in contact with a peripheral edge portion of the first vessel wall, and has a bulge shape protruding relative to the first vessel wall. The first vessel wall is flat at its section other than a region where the first vessel wall is in contact with at least a charge/discharge port.

Abstract: The present disclosure provides an in vitro tubular blood brain barrier mimic used to model drug transport across the brain capillary endothelial barrier cells to neurons. In one embodiment the stack is comprised of interior neuro endothelial capillary cells, extracellular matrix, porous polymeric hollow fiber, exterior extracellular matrix, and neuron astrocytes. The tubular human blood brain barrier mimic is used to test molecular transport and effects of drug candidates across the multilayer stack.

Abstract: A device and methodology for concomitant ejection and suction of a perfusate (CESOP) within a region of interest without spillover of the perfusate to a juxtaposed anatomical region. An inflow line, connected to a perfusate cistern, is coupled to an outflow line, connected to outflow control mechanisms, via an elongate rigid support. Perfusate can flow through the inflow line to the region of interest, and nearly simultaneously, the outflow line can suction the perfusate out of the region of interest. The amount of time that the perfusate remains in the region of interest is sufficient to take effect in the region of interest. The rigid support helps control the inflow and outflow lines. A micromanipulator can also be used to control the lines and application tip thereof. Either or both lines may also have adjustable flow rates therethrough and may include stoppers/regulators.

Abstract: Provided is a liquid feeding device which can improve collection efficiency of fine particles on an individual particle basis while improvement of workability is realized. A liquid feeding device includes: an introduction portion disposed on one end portion of the liquid feeding device, and configured to introduce a liquid into a closed space; a discharge portion disposed on another end portion of the liquid feeding device, and configured to discharge the liquid supplied into the closed space; and an upper wall provided so as to oppose the upper surface of the chip, and configured to define the closed space. The upper wall has an inclined surface which is provided such that a gap formed between the inclined surface and the chip is reduced from an introduction portion side toward a discharge portion side.

Abstract: An apparatus for electroporation of biological cells is provided. The apparatus includes a sample container having an insulator chamber for holding the cells. The sample container has a first electrode and a second electrode to provide electrical connection for electroporation. The insulator chamber is configured to contain at least one cell monolayer. The apparatus also includes a pulse generator that can generate a predetermined pulse for electroporation of the cells.

Abstract: Disclosed is an in vitro exposure system that may radiate a uniform field having a constant wavefront to an experimental cell container and expose each cell container to a same electromagnetic field.

Abstract: The present invention provides an apparatus for applying electrical stimulation to cells in a liquid culture medium without using electrodes immersed in the liquid culture medium. This apparatus is an electrical stimulation apparatus A for electrically stimulating cells in a liquid culture medium (2). A liquid culture medium vessel (3) includes: a ring-shaped recess (7) for holding the liquid culture medium (2); and a through hole (6) formed within the ring-shaped recess (7). A magnetic core (1) is made of a magnetic material and disposed such that a portion thereof is inserted through the through hole (6) of the liquid culture medium vessel (3). An excitation coil (5) is wound around the magnetic core (1). A coil power supply (8) supplies a varying current to the excitation coil (5).

Abstract: A composite sensor assembly for monitoring bio-processes which is suitable for use with a polymeric bioprocess vessel or with downstream equipment, and comprises: i) a port comprising a high surface tension thermoplastic having a hollow tubular portion and a base plate portion, the base plate being fusibly sealable to the bioreactor vessel at a hole in the wall thereof; ii) a generally opaque polymeric monitoring sensor assembly including electrical, and/or optical measurement components. The sensor assembly fits inside the bore of the hollow tubular portion of the port, and is adhesively retained therein.

Abstract: The present invention relates to a suspension cell culture monitoring apparatus including a chamber unit, which is a portion submerged in an incubator in which cells are cultured, that has at least a portion opened by an opening so that a culture medium and suspension cells are allowed to pass therethrough, a lens unit configured to zoom in or zoom out the suspension cells moving through the opening of the chamber unit, which are visible through an observation area of the chamber unit, by adjusting a focus, and a sensor unit configured to acquire an image in which the suspension cells visible through the lens unit are present.

Abstract: Methods of purifying a virus from a virus-infected cell lysate using three-phase partitioning (TPP) are disclosed. The methods comprise a first round of TPP, including mixing a cell lysate comprising a virus with ammonium sulfate and t-butanol, and separating the mixture, thereby forming a first aqueous phase, a first organic phase, and a first interphase. The first aqueous phase can comprise the virus, which can be subjected to a second round of TPP, resulting in a second aqueous phase, a second organic phase, and a second interphase. The second interphase can comprise highly purified virus. The methods can comprise subjecting a first aqueous phase to further purification by column chromatography or density gradient centrifugation. Purification of AAV, including AAV2, AAV5 and AAV6, from lysates of infected insect cell cultures is demonstrated. TPP-purified AAV particles infect at least as well as those prepared by standard methods.

Abstract: A method for producing differentiated cells from pluripotent stem cells by an embryoid body (EB) method, including the steps of: (1) culturing pluripotent stem cells by using a culture bag having a perfluoropolymer on its inner surface to thereby form an embryoid body; and (2) inducing differentiation of the pluripotent stem cells contained in the embryoid body obtained in step (1) to thereby obtain differentiated cells.

Abstract: Provided is a method for inducing CD4?CD8+ T cells having an antigen specific cytotoxic activity from pluripotent stem cells, comprising the steps of: (1) differentiating pluripotent stem cells to give a cell culture comprising CD4?CD8? T cells and CD4+CD8+ T cells, (2) removing CD4?CD8? cells from the cell culture obtained in step (1), and (3) differentiating the CD4+CD8+ cells in the cell culture into CD4?CD8+ T cells.

Abstract: The present invention relates to a method for ex vivo generating and expanding MHCII restricted CD4+ Foxp3+ regulatory T cells, and therapeutic uses thereof. The inventors here demonstrated the optimal conditions for inducing Foxp3 expression in naive CD3+ CD4+ TCR??+ MHCII restricted T following polyclonal or following antigen-specific activation. They also developed an experimental procedure to generate autologous CD8+ T cell lines functionally committed to lyse tumor-antigen specific FOXP3 expressing TCR??+ MHCII restricted T cells, pathogenic CD4+ T cells that favour tumor cell immune evasion. In particular, the present invention relates to a method for generating ex vivo MHCII restricted CD4+ Foxp3+ regulatory T cells having the following phenotype: CD3+ CD4+ Foxp3+.

Abstract: The present invention relates to a method for ex vivo generating and expanding ?? Foxp3+ regulatory T cells, and therapeutic uses thereof. The inventors performed the induction of Foxp3? expression in ex vivo human induced tumor-antigen specific CD4+ TCR?? unrestricted T cells and the induction of autologous CD8-mediated T-cell responses against tumor-antigen specific FOXP3 expressing CD4+ TCR?? unrestricted T cells. The inventors developed a method to ex vivo generated and expanded antigen specific Foxp3 expressing CD3+ TCR?? unrestricted T cells, committed to exclusively exert regulatory activity, whichever culture condition of stimulation is. In particular, the present invention relates to a method for generating ex vivo ?? Foxp3+ regulatory T cells having the following phenotype: CD3+ TCR?? Foxp3+.

Abstract: The invention includes compositions and methods related to erythroid cells comprising exogenous RNA encoding a protein. The exogenous RNA can comprise a heterologous untranslated region comprising a regulatory element. Alternatively or in combination, the exogenous RNA can comprise chemical modifications.

Abstract: Described herein are methods of generating induced muscle progenitor cells (iMPCs) and uses thereof. Embodiments further provide for methods of promoting muscle regeneration and/or repair and methods of treating a muscle disease or disorder.

Abstract: The present application provides a method for rejuvenating cells, characterized in that providing a cell to culture in a composition for a suitable period of time of time, wherein the composition is obtained by adding a chitosan solution to a cultural environment. The present application further provides a composition for rejuvenating cells, comprising a chitosan solution in a cultural environment.

Abstract: The invention is in the field of cell culturing. More specifically, it is in the field of generating and expanding myogenic cells from induced pluripotent stem (i PS) cells. The invention relates inter alia to cells generated and expanded via such a method, a growth medium specifically suited for the purpose of expanding isolated myogenic cells, and methods for screening compounds on cell structures such as myotubes and myofibers.

Abstract: This application describes liver stem cells (LSC), and differentiated hepatocytes, cholangiocytes, and 3D cellular structures derived therefrom. Methods for producing LSC and mature, differentiated hepatocytes and cholangiocytes in culture are provided. Also provided are cell culture systems and cell culture media for producing a homogenous population of liver stem cells that remain in an undifferentiated state over multiple passages in culture. The LSC and methods are useful for producing homogenous populations of hepatocytes and cholangiocytes for downstream applications. The LSC can be transplanted into subjects to treat liver diseases.

Abstract: The present disclosure provides a model of human fibrolamellar hepatocellular carcinoma (FL-HCC) cells maintained as a transplantable tumor line in a host and a method to establish a transplantable human FL-HCC tumor line. Methods of ex vivo cultures of the FL-HCC are provided. Methods of diagnosing and treating FL-HCC tumors are also provided.

Abstract: The disclosure relates to the enrichment and expansion of rare cells in blood such as circulating tumor cells (CTCs), cancer stem cells [CSCs] and other rare circulating cells. The microwells promote interactions between patient-derived CTCs and blood cells, allowing expansion of CTCs without the need for pre-enrichment or additional growth supplements. The cultured cells can be selected for propagation from single cells and have utility in drug screening, diagnostics and prognostics. The disclosure also includes a system comprising a cell enrichment device for enriching CTCs and CSCs and a device adapted to co-operate with the cell enrichment device to allow the testing of one or more agents, for example therapeutic or diagnostic agents.

Abstract: The present invention is directed towards compositions and methods of culturing cancer cells, with the methods comprising culturing cancer cells in the presence a cell culture medium while inhibiting the activity of Rho kinase (ROCK) in the cells during culturing.

Abstract: The present invention provides methods and compositions for inducing pluripotency in differentiated mammalian cells. In particular, the methods include mechanically aggregating the cells into discrete masses or embryoid-like bodies and treated them with a small molecule compound. Provided herein are the compositions of the compounds which are derived from programin (e.g., reversine).

Abstract: A method of generating protein-induced pluripotent stem cells by delivering bacterially expressed reprogramming proteins into nuclei of starting somatic cells using the QQ-protein transduction technique, repeating several cell reprogramming cycles for creating reprogrammed protein-induced pluripotent stem cells, moving the reprogrammed cells into a feeder-free medium for expansion, and expanding and passaging the reprogrammed cells in a whole dish for generating homogeneous piPS cells. Also provided are the piPCS cells formed using this method and uses thereof.

Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.

Abstract: The present invention discloses a formate dehydrogenase mutant with improved enzyme activity and stability and a construction method thereof, which belongs to the technical field of genetic engineering. The mutant of the present invention is obtained by mutating alanine at a 10th site to cysteine based on the amino acid shown in SEQ ID NO. 2. The specific enzyme activity of the mutant enzyme obtained by the present invention is improved by 1.3 times compared with that before the mutation, a half-life period (t1/2) at 60° C. is increased by 6.8 times compared with that in the mutation period, the copper ion tolerance is increased by 30 times compared with that before the mutation, and when pH is 4, the stability is improved by 2.0 times, and the catalytic efficiency is increased by 1.4 times.

Abstract: Compositions and methods are provided for novel Cas9 systems, including, but not limiting to, novel guide polynucleotide/Cas9 endonucleases complexes, single or dual guide RNAs, guide RNA elements, and Cas9 endonucleases. The novel Cas9 systems described herein are derived from a CRISPR-Cas locus that differs from type II CRISPR-Cas loci, in that the novel CRISPR-Cas locus described herein contains multiple CRISPR arrays flanking a tracrRNA encoding region and cas9 gene and does not contain a cas1 gene and a cas2 gene. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.

Abstract: CRISPR/Cas9 methods are provided where a guide RNA is engineered to self-target and inactivate a nucleic acid encoding the guide RNA itself.

Abstract: The present invention relates to compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity for use in e.g. animal feed. The present invention further relates to polypeptides having beta-galactosidase activity, polypeptides having galactanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.