Patents Issued in May 14, 2020
  • CELL LYSIS AND BIOMOLECULE DISASSOCIATION SYSTEM AND METHOD FOR MASS SPECTROMETRY OR OTHER ANALYSIS
    Publication number: 20200148996
    Abstract: Apparatus and method for lysing and recovering released material, such as proteins or other biomolecules. Sample material including cells may be at least partially frozen, thawed, cells lysed and biomolecules recovered in a single conduit, e.g., as the sample flows through the conduit. The conduit may include different zones for sample treatment, including a freezing zone, thawing zone, lysing/disassociation zone, etc., and protein or other recovered material may be delivered directly from the conduit to analysis equipment, such as a mass spectrometer. This allows a flow-through type processing of cells that avoids handling and transfer of sample material between different sample holders.
    Type: Application
    Filed: October 29, 2019
    Publication date: May 14, 2020
    Applicant: Covaris, Inc.
    Inventors: James A. LAUGHARN, JR., William Murray SKEA, Hans-Ulrich THOMANN, Todd Anthony BASQUE
  • METHOD OF PRODUCING HIGH FUCOXANTHIN-CONTAINING ALGA
    Publication number: 20200148997
    Abstract: Provided is a method of producing a high fucoxanthin-containing alga, the method including culturing an alga containing fucoxanthin by irradiating the alga with light from a light source capable of emitting at least light corresponding to green. This method is capable of increasing fucoxanthin production.
    Type: Application
    Filed: November 8, 2019
    Publication date: May 14, 2020
    Applicants: South Product Ltd., JXTG Nippon Oil & Energy Corporation
    Inventors: Takayuki KANESHIRO, Masahiko IHA, Toshiyuki TAKAHASHI, Takashi ISHIBASHI
  • Method For Production of an Engineered Liquid
    Publication number: 20200148998
    Abstract: A method for the production of an engineered liquid for use in a system to duplicate a flammable gas. The engineered liquid facilitates duplicating a quantity of almost any selected or desired flammable gas. The method comprises the following steps: place a bacteria into a container; add an algae into the container; add water to the bacteria and algae to create a mutated bacteria solution; add a quantity of bone powder; add a quantity of nutrients and water; and allow the mutated bacteria solution to culture.
    Type: Application
    Filed: August 3, 2019
    Publication date: May 14, 2020
    Inventors: Faramaz Fred Faramarzi, Sabrina Faramarzi
  • CELL CULTERING MATERIALS
    Publication number: 20200148999
    Abstract: A material for binding to a cell culturing protein is disclosed. The material contains a bulk-modified elastomer comprising a plurality of fatty acid moieties covalently bound to the elastomer bulk, wherein the carboxylic acid groups of said moieties are available to provide said binding. Also disclosed are a fluidic device module, a cell culturing scaffold, a fluidic device, the method of synthesizing such a material and a drug testing method. With such a material, a (monolithic) fluidic device module may be manufactured in as few as a single step injection molding process.
    Type: Application
    Filed: July 6, 2018
    Publication date: May 14, 2020
    Inventors: LUCAS JOHANNES ANNA MARIA BECKERS, JAN CORNELIS KRIEGE
  • THREE-DIMENSIONAL SCAFFOLD STRUCTURES WITH SELECTIVELY FUNCTIONALIZED POLYMER SYSTEMS, THEIR USE AND PROCESS FOR THEIR MANUFACTURE
    Publication number: 20200149000
    Abstract: A three-dimensional scaffold structure and a process for its manufacture. The scaffold structure has a polymer system obtainable by photostructuring a starting material containing precursor molecules or inorganic polymers thereof having an organically polymerizable radical with at least one C?C double bond, an inorganically polymerizable silane-based radical and a functional group or derivative thereof.
    Type: Application
    Filed: November 14, 2019
    Publication date: May 14, 2020
    Inventors: Herbert Wolter, Somchith Nique, Sebastian Hasselmann, Caroline Kopittke, Doris Heinrich, Johannes Schwaiger
  • Thermoresponsive Microcarrier System and Uses Thereof
    Publication number: 20200149001
    Abstract: There is provided a polymeric microsphere comprising a thermally responsive monomer crosslinked with a functional group monomer, wherein the functional group monomer comprises at least one of a carboxylic acid functional group or an amine functional group. The thermally responsive monomer is preferably N-isopropylacrylamide (NIPAM), and the microspheres preferably comprise a coating of polymerized catecholamines (e.g. DOPA). There is also provided a method of preparing the polymeric microsphere and uses of the polymeric microsphere in culturing, harvesting, or expanding stem cells or stromal cells. Preferably, the cells, e.g. hMSCs (human mesenchymal stem/stromal cells), are expanded or harvested in serum-free and xeno-free medium.
    Type: Application
    Filed: January 22, 2018
    Publication date: May 14, 2020
    Inventors: Jackie Y. Ying, Leng Leng Chng, Daniele Zink, Aysha Farwin
  • CONDITIONED MEDIUM AND EXTRACELLULAR MATRIX COMPOSITIONS FROM CELLS CULTURED UNDER HYPOXIC CONDITIONS
    Publication number: 20200149002
    Abstract: The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.
    Type: Application
    Filed: January 17, 2020
    Publication date: May 14, 2020
    Inventors: Gail K. Naughton, Francis C. Zeigler, Mark Baumgartner, Kyle Nickey
  • METHODS FOR THE PRODUCTION OF RETINAL CELLS
    Publication number: 20200149003
    Abstract: The general inventive concepts contemplate methods and compositions for modifying certain cell lines to facilitate the production of retinal tissue from human induced pluripotent stem cells. Human induced pluripotent stem cells can be cultured to develop into retinal organoids, among other cells types. The retinal organoids play a role in modeling both human retinal development and retinal disease. The methods and compositions discussed herein provide means to measure and monitor human induced pluripotent stem cells during development.
    Type: Application
    Filed: April 22, 2019
    Publication date: May 14, 2020
    Inventors: Phuong T. Lam, Katia Del Rio-Tsonis, Michael L. Robinson
  • COMPOSITIONS AND METHODS FOR OBTAINING FUNCTIONAL BASAL-LIKE CELLS
    Publication number: 20200149004
    Abstract: The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or cells through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting functional basal-like cells from pluripotent stem cell-derived lung bud tip progenitor organoid tissue through activation of SMAD signaling via activation of TGF?1 (and/or the TGF?signaling pathway) and BMP4 (and/or the BMP signaling pathway).
    Type: Application
    Filed: November 8, 2019
    Publication date: May 14, 2020
    Inventors: Jason Spence, Alyssa Miller
  • THE METHOD OF AUTOLOGOUS PRIMARY HAIR FOLLICLES PREPARATION IN 3D CULTURE
    Publication number: 20200149005
    Abstract: The present invention relates to a method for the preparation of autologous human primary hair follicles in 3D cultures, comprising the isolation of primary fetal follicles; isolation of the patient's hair follicle cells; isolation of skin cells of the patient's scalp; extraction of growth factors from fetal follicle cells; the fibrin gel creation that contains growth factors of fetal follicles; sandwich cultivation of patient's hair follicle cells and skin of the patients scalp on or into fibrin gel that contains growth factors of fetal follicles; separation from fibrin gel the patients primary hair follicles, which can be used to treat baldness as an autologous graft.
    Type: Application
    Filed: May 3, 2019
    Publication date: May 14, 2020
    Inventors: Abhijit Bopardikar, Oleksandr Kukharchuk, Padma Priya Anand Baskaran, Andrii Kukharchuk, Sunil Pophale, Rohit R. Kulkarni
  • B-cell cultivation method
    Publication number: 20200149006
    Abstract: Herein is reported a method for co-cultivating B-cells in the presence of phorbol myristate acetate, IL-1beta, TNFalpha, IL-2, IL-10 and IL-6.
    Type: Application
    Filed: June 27, 2019
    Publication date: May 14, 2020
    Applicant: Hoffmann-La Roche Inc.
    Inventor: Friederike JUNG
  • EX VIVO ANTIBODY PRODUCTION
    Publication number: 20200149007
    Abstract: The present invention provides means and methods for producing improved ex vivo B cell cultures with a short doubling time.
    Type: Application
    Filed: September 19, 2019
    Publication date: May 14, 2020
    Inventors: PAULA MARIA WILHELMINA VAN HELDEN, Mark Jeroen KWAKKENBOS, Hergen Spits, Tim Beaumont
  • UTILIZATION OF CD39 AND CD103 FOR IDENTIFICATION OF HUMAN TUMOR REACTIVE T CELLS FOR TREATMENT OF CANCER
    Publication number: 20200149008
    Abstract: Methods are disclosed for treating a subject with a tumor. These methods include administering to the subject a therapeutically effective amount of CD8+CD39+CD103+ T cells. Methods also are disclosed for isolating a nucleic acid encoding a T cell receptor (TCR) that specifically binds a tumor cell antigen. These methods include isolating CD8+CD39+CD103+ T cells from a sample from a subject with a tumor expressing the tumor cell antigen, and cloning a nucleic acid molecule encoding a TCR from the CD8+CD39+CD103+ T cells. In addition, methods are disclosed for expanding CD8+CD39+CD103+ T cells. In additional embodiments, methods are disclosed for determining if a subject with a tumor will respond to a checkpoint inhibitor. The methods include detecting the presence of CD8+CD39+CD103+ T cells in a biological sample from a subject.
    Type: Application
    Filed: May 4, 2018
    Publication date: May 14, 2020
    Applicants: Providence Health & Services - Oregon, AgonOx, Inc.
    Inventors: Andrew D. Weinberg, Ryan Montler, Thomas Duhen, Rebekka Duhen
  • METHODS AND COMPOSITIONS FOR MODULATING CYTOTOXIC LYMPHOCYTE ACTIVITY
    Publication number: 20200149009
    Abstract: The subject matter disclosed herein is generally directed to novel CD8+ tumor infiltrating lymphocyte (TIL) subtypes associated with response to immunotherapy treatment. Specifically, the subtypes are associated with checkpoint blockade therapy. Moreover, the subject matter disclosed herein is generally directed to methods and compositions for use of the subtypes. Also, disclosed herein are gene signatures and markers associated with the subtypes and use of said signatures and markers. Further disclosed are therapeutic methods of using said gene signatures and immune cell subtypes. Further disclosed are pharmaceutical compositions comprising populations of CD8+ TILs enriched for a specific subtype.
    Type: Application
    Filed: July 13, 2018
    Publication date: May 14, 2020
    Inventors: Aviv Regev, Ana C. Anderson, Vijay K. Kuchroo, Sema Kurtulus, Asaf Madi
  • METHODS OF T CELL EXPANSION AND ACTIVATION
    Publication number: 20200149010
    Abstract: The present disclosure relates to methods, cells, and compositions for preparing T cell populations and compositions for adoptive cell therapy. In particular, provided herein are methods for efficiently expanding and activating T cell populations for genetic engineering and adoptive T cell immunotherapies. Also provided are cells and compositions produced by the methods and methods of their use.
    Type: Application
    Filed: January 7, 2020
    Publication date: May 14, 2020
    Inventors: Peiman Hematti, Debra Bloom
  • METHODS OF EXPANDING HEMATOPOIETIC STEM CELLS, COMPOSITIONS, AND METHODS OF USE THEREOF
    Publication number: 20200149011
    Abstract: Described herein are methods of expanding a population of hematopoietic stem cells by contacting the population of hematopoietic stem cells with an effective amount of an inhibitor of G-protein coupled receptor 65 (GPR65) and providing a population of expanded, substantially undifferentiated hematopoietic stem cells. Exemplary GPR65 inhibitors include siRNAs and certain sphingolipids. Also described are populations of expanded hematopoietic stem cells produced by the methods, media and kits containing GPR65 inhibitors, and methods for administering an expanded population of hematopoietic stem cells to patients.
    Type: Application
    Filed: January 22, 2020
    Publication date: May 14, 2020
    Inventors: EMERY H. BRESNICK, XIN GAO, KIRBY D. JOHNSON
  • CD39 STROMAL STEM CELLS METHODS OF ISOLATION AND USE
    Publication number: 20200149012
    Abstract: Disclosed herein are methods of isolating SDC2+ stromal stem cells and exosomes from SDC2+ stromal stem cells by expression of surface marker CD39. Also disclosed herein are stromal stem cells and exosomes isolated by said methods.
    Type: Application
    Filed: July 12, 2018
    Publication date: May 14, 2020
    Inventors: Stephen J. ELLIMAN, Jack KAVANAUGH, Larry A. COUTURE, Lisa O'FLYNN
  • Method of Selecting Viruses for the Treatment of Cancer
    Publication number: 20200149013
    Abstract: The present disclosure presents a method of genetically selecting an RNA virus to treat cancer. The method involves choosing an RNA virus, exposing it to the cancer cells, followed by exposure to non-target cells to select for the RNA virus that does not bind non-target cells, followed by repeating the process until a treatment RNA virus is obtained.
    Type: Application
    Filed: November 13, 2018
    Publication date: May 14, 2020
    Inventor: Chris Coleclough
  • ADENOVIRAL ASSEMBLY METHOD
    Publication number: 20200149014
    Abstract: Methods of assembling modified adenoviruses, libraries of adenoviral gene modules and compositions thereof are provided herein.
    Type: Application
    Filed: January 22, 2020
    Publication date: May 14, 2020
    Applicant: Salk Institute for Biological Studies
    Inventors: Clodagh O'Shea, Colin Powers
  • Novel Method of Protein Purification
    Publication number: 20200149015
    Abstract: The present disclosure provides methods for releasing intracellular proteins. The method allows isolation of the protein of interest from the cell without the requirement for mechanical disruption of the cells, without the need for isolation of the cells from the culture media, and without the need for removal of the cells from the culture media.
    Type: Application
    Filed: July 15, 2019
    Publication date: May 14, 2020
    Inventor: Stephen Hawley
  • SUPEROXIDE DISMUTASE GENE AND ITS CODING PROTEIN
    Publication number: 20200149016
    Abstract: The present invention discloses a superoxide dismutase gene and a protein encoded thereby, the DNA sequence of the superoxide dismutase gene is shown in SEQ ID NO. 1, and the encoded protein sequence of the superoxide dismutase gene is shown in SEQ ID NO 2. The superoxide dismutase encoded by the SOD gene of the invention has good heat resistance and freeze-thaw resistance, and can be widely applied in the fields of cosmetics, food, medicine, environmental protection and the like.
    Type: Application
    Filed: January 22, 2020
    Publication date: May 14, 2020
    Inventor: JUN LIN
  • THERAPEUTICS FOR GLYCOGEN STORAGE DISEASE TYPE III
    Publication number: 20200149017
    Abstract: This invention provides a range of translatable polynucleotide and oligomer molecules for expressing a human amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), or a fragment thereof having AGL activity. The polynucleotide and oligomer molecules are expressible to provide the human AGL or a fragment thereof having AGL activity. The molecules can be used as active agents to express an active polypeptide or protein in cells or subjects. The agents can be used in methods for ameliorating, preventing, delaying onset, or treating a disease or condition associated with reduced activity of amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL) in a subject.
    Type: Application
    Filed: May 31, 2018
    Publication date: May 14, 2020
    Inventors: Kiyoshi TACHIKAWA, Carlos Gustavo PEREZ-GARCIA, Padmanabh CHIVUKULA, Hari Prakash BHASKARAN, Christian W. COBAUGH, Sean Christopher DAUGHERTY
  • DNA POLYMERASE WITH INCREASED GENE MUTATION SPECIFICTY AND PCR BUFFER COMPOSITION FOR INCREASING ACTIVITY THEREOF
    Publication number: 20200149018
    Abstract: A DNA polymerase in which a mutation is induced at a specific amino acid position to increase gene mutation specificity, a nucleic acid sequence encoding the polymerase, a vector comprising the nucleic acid sequence, and a host cell transformed with the vector are disclosed. Provided are a method for in vitro detecting one or more gene mutations or SNPs in one or more templates by using a DNA polymerase having increased gene mutation specificity, a composition for detecting a gene mutation or SNP comprising the DNA polymerase, and a PCR kit comprising said composition. Furthermore, provided are a PCR buffer composition for increasing the activity of a DNA polymerase having increased gene mutation specificity and a PCR kit for detecting a gene mutation or SNP comprising the PCR buffer composition and/or the DNA polymerase having increased gene mutation specificity.
    Type: Application
    Filed: May 31, 2018
    Publication date: May 14, 2020
    Inventors: Byung Chul LEE, IL HYUN PARK, Huy Ho LEE
  • TREATMENT OF MUCOPOLYSACCHARIDOSIS II WITH RECOMBINANT HUMAN IDURONATE-2-SULFATASE (IDS) PRODUCED BY HUMAN NEURAL OR GLIAL CELLS
    Publication number: 20200149019
    Abstract: Compositions and methods are described for the delivery of recombinant human iduronate-2-sulfatase (IDS) produced by human neuronal or glial cells to the cerebrospinal fluid of the central nervous system (CNS) of a human subject diagnosed with mucopolysaccharidosis II (MPS II).
    Type: Application
    Filed: April 13, 2018
    Publication date: May 14, 2020
    Applicant: REGENXBIO INC.
    Inventors: Stephen Yoo, Rickey Robert Reinhardt, Curran Matthew Simpson, Zhuchun Wu
  • HIGH-FIDELITY CAS9 VARIANTS AND APPLICATIONS THEREOF
    Publication number: 20200149020
    Abstract: To address the limitations deriving from the unspecific genomic cleavages of the Streptococcus pyogenes Cas9 (SpCas9) and to identify variants with higher cleavage fidelity, the present invention describes a yeast-based assay which allows to simultaneously evaluate the on- and off-target activity towards two engineered genomic targets. The screening of SpCas9 variants obtained by random mutagenesis of the Red-II domain allowed the identification of hits with increased on/off ratios. The best performing nuclease, evoCas9, was isolated through the combination of the identified mutations within a single variant. Side by side analyses with previously reported rationally designed variants demonstrated a significant improvement in fidelity of evoCas9 of the present invention.
    Type: Application
    Filed: February 14, 2018
    Publication date: May 14, 2020
    Applicant: UNIVERSITA' DEGLI STUDI DI TRENTO
    Inventors: Anna CERESETO, Antonio CASINI, Gianluca PETRIS, Alberto INGA, Michele OLIVIERI
  • SYSTEM AND METHOD FOR GENOME EDITING
    Publication number: 20200149021
    Abstract: The invention relates to the field of genetic engineering. In particular, the present invention relates to a novel eukaryotic genome editing system and method. More specifically, the present invention relates to a CRISPR-Cpf1 system capable of efficiently editing a genome of a eukaryotic cell and the use thereof.
    Type: Application
    Filed: April 10, 2018
    Publication date: May 14, 2020
    Inventors: Wei LI, Qi ZHOU, Fei TENG
  • TARGET-SPECIFIC CRISPR MUTANT
    Publication number: 20200149022
    Abstract: The present invention relates to an artificially engineered CRISPR/Cas9 system. More particularly, the present invention relates to an artificially engineered CRISPR enzyme having enhanced target specificity and a use of an artificially engineered CRISPR/Cas9 system including the same enzyme in genome and/or epigenome manipulation or modification, genome targeting, genome editing, and in vitro diagnosis, etc.
    Type: Application
    Filed: July 6, 2018
    Publication date: May 14, 2020
    Inventors: Young-Hoon KIM, Min Hee JUNG, Joonsun LEE, Eunji SHIN, Kang In LEE, Seokjoong KIM, Jeongjoon LEE
  • Engineered CRISPR-Cas9 nucleases with Altered PAM Specificity
    Publication number: 20200149023
    Abstract: Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.
    Type: Application
    Filed: November 18, 2019
    Publication date: May 14, 2020
    Inventors: J. Keith Joung, Benjamin Kleinstiver
  • Engineered CRISPR-Cas9 Nucleases
    Publication number: 20200149024
    Abstract: Engineered CRISPR-Cas9 nucleases with improved specificity and their use in genomic engineering, epigenomic engineering, genome targeting, and genome editing.
    Type: Application
    Filed: January 6, 2020
    Publication date: May 14, 2020
    Inventors: J. Keith Joung, Benjamin Kleinstiver, Vikram Pattanayak
  • STREPTOCOCCUS BACTERIOPHAGE LYSINS FOR DETECTION AND TREATMENT OF GRAM POSITIVE BACTERIA
    Publication number: 20200149025
    Abstract: The present invention provides methods, compositions and articles of manufacture useful for the prophylactic and therapeutic amelioration and treatment of gram-positive bacteria, including Streptococcus and Staphylococcus, and related conditions. The invention provides compositions and methods incorporating and utilizing Streptococcus suis derived bacteriophage lysins, particularly PlySs2 and/or PlySs1 lytic enzymes and variants thereof, including truncations thereof. Methods for treatment of humans are provided.
    Type: Application
    Filed: December 9, 2019
    Publication date: May 14, 2020
    Inventors: Vincent A. Fischetti, Jonathan Schmitz, Daniel Gilmer, Chad Euler
  • RNAS FOR WOUND HEALING
    Publication number: 20200149026
    Abstract: The present invention relates to an RNA encoding a therapeutic protein, in particular a collagenase, growth factor, cytokine, receptor, chaperone or signal transduction inhibitor. In particular, the present invention relates to RNA suitable for treatment of wounds, specifically for promoting wound healing. The present invention concerns such RNA as well as pharmaceutical compositions and kits and combinations comprising the RNA. Furthermore, the present invention relates to the RNA, pharmaceutical compositions, kits as disclosed herein for use in the treatment of wounds, specifically for promoting wound healing.
    Type: Application
    Filed: December 8, 2017
    Publication date: May 14, 2020
    Inventors: Nigel HORSCROFT, Marion PÖNISCH, Christine WEINL
  • Calcium Independent Pectinases with Improved Thermostability
    Publication number: 20200149027
    Abstract: The invention is in the field of protein chemistry, in particular in the field of enzymology. It provides pectinases, i.e. polypeptides with pectin-degrading properties. In particular the invention provides polypeptides with pectate lyase activity (EC 4.2.2.2). Enzymes according to the invention have improved properties, such as improved thermostability and decreased calcium dependence.
    Type: Application
    Filed: December 8, 2016
    Publication date: May 14, 2020
    Inventors: Klara Birikh, Anu Minna Maaret Suonpää
  • HISTIDYL-TRNA SYNTHETASE-FC CONJUGATES
    Publication number: 20200149028
    Abstract: The present invention provides histidyl-tRNA synthetase and Fc region conjugate polypeptides (HRS-Fc conjugates), such as HRS-Fc fusion polypeptides, compositions comprising the same, and methods of using such conjugates and compositions for treating or diagnosing a variety of conditions. The HRS-Fc conjugates of the invention have improved controlled release properties, stability, half-life, and other pharmacokinetic and biological properties relative to corresponding, unmodified HRS polypeptides.
    Type: Application
    Filed: September 20, 2019
    Publication date: May 14, 2020
    Inventors: Chi-Fang WU, Darin Lee, Jeffry D. Watkins, Kristi Piehl, Kyle P. Chiang, Marc Thomas, Minh-Ha Do, Ying Buechler, John D. Mendlein
  • IMMOBILIZED CYCLOALIPHATIC PEPTIDE ACYLTRANSFERASE AND PREPARATION METHOD AND USES THEREOF
    Publication number: 20200149029
    Abstract: Disclosed in the present invention are an immobilized cycloaliphatic peptide acyltransferase and a preparation method and use thereof. The cycloaliphatic peptide acyltransferase is immobilized on a carrier; the cycloaliphatic peptide acyltransferase is derived from natural or artificial mutants or variants thereof, or can be obtained by introducing a foreign cyclic acyltransferase gene and transforming thereafter; the material of the carrier is selected from an inorganic carrier or a polypropylene resin carrier. Also disclosed in the present invention are the preparation method for the immobilized cycloaliphatic peptide acyltransferase and uses thereof.
    Type: Application
    Filed: January 13, 2020
    Publication date: May 14, 2020
    Inventors: Shidong LIU, Zhaoli ZHANG, Xiaoming JI
  • MOBILE DEVICE FOR THE ISOLATION OF NUCLEIC ACIDS
    Publication number: 20200149030
    Abstract: The invention relates to a mobile device system, comprising a hand-held device and a test kit for the mobile isolation of nucleic acids. The hand-held device comprises at least one sample block for inserting sample containers, a sample block holder with boreholes or recesses for accommodating the sample blocks, a device base with electronic control units for the sample blocks, a voltage source and a connection to the sample block holder, as well as a test kit for the isolation of nucleic acids. The hand-held device is characterized in that the sample blocks can be removed from the sample block holder and the sample block holder can be removed from the device base.
    Type: Application
    Filed: January 9, 2020
    Publication date: May 14, 2020
    Applicant: AJ Innuscreen GmbH
    Inventors: Timo HILLEBRAND, Claus KNIPPSCHILD
  • HIGHLY PARALLEL ASSAYS FOR SIMULTANEOUS IDENTIFICATION OF ANTIBODY SEQUENCES AND BINDING PARTNERS
    Publication number: 20200149031
    Abstract: The present invention relates to methods for isolating the sequences of an antibody that reacts with a disease related antigen, e.g., an autoantigen, without knowing the identity of the antigen (sequence or structural epitope) a priori. The methods can also be used to identify an antigen that mediates a disease state, e.g., an autoantigen implicated in an autoimmune disorder or a tumor response.
    Type: Application
    Filed: December 21, 2016
    Publication date: May 14, 2020
    Inventors: Ester FALCONER, Carl Lars Genghis HANSEN, Veronique LECAULT, Kathleen LISAINGO, Kevin Albert HEYRIES
  • A METHOD OF GENERATING A SYNTHETIC ANTIBODY LIBRARY, SAID LIBRARY AND APPLICATION(S) THEREOF
    Publication number: 20200149032
    Abstract: The present disclosure relates to a method of generating an antibody library, not limiting to a synthetic antibody gene expression library built on pool of consensus nucleic acid sequences by using codon replacement technology. The present disclosure also relates to a synthetic antibody library generated by employing the method of the present disclosure and application(s) of said antibody library.
    Type: Application
    Filed: January 19, 2017
    Publication date: May 14, 2020
    Inventors: Sohang CHATTERJEE, Kavitha IYER RODRIGUES, Maloy GHOSH, Sunit MAITY, Divya UNNIKRISHNAN, Yogendra MANJUNATH BANGALORE MUNIRAJU, Sathyabalan MURUGESAN, Pavithra MUKUNDA, Bhargav PRASAD, Veeresha KAMANAGOWDA, Sanghamitra BHATTACHARJEE, Pravin KUMAR DAKSHINAMURTHY, Vivek HALAN, Sankaranarayanan SRINIVASAN, Anuradha HORA, Bairavabalakumar NATARAJAN, Karthika NAIR, Aswini THANIGAIVEL, MALIWALAVE Amol, Bharath RAVINDRA SHENOY, Sahana BHIMA RAO, Subhra PRAKASH CHAKRABARTY, Ashvini KUMAR DUBEY, Amir KHAN, Ankurina SHARMA, Rashmi SHARMA, Anurag TIWARI, Santosh KUMAR, Shivani PATEL, Nikitha MARKANDA
  • MUTANT VIRAL CAPSID LIBRARIES AND RELATED SYSTEMS AND METHODS
    Publication number: 20200149033
    Abstract: Provided are mutant viral capsid cell libraries, individual cells of such libraries, systems, vectors, and methods for generating the cell libraries, and methods of use thereof to screen for mutant viral capsids with desired characteristics.
    Type: Application
    Filed: January 23, 2020
    Publication date: May 14, 2020
    Inventors: Christopher CHAVEZ, Mehdi GASMI, Annahita KERAVALA, Thomas W. CHALBERG
  • Methods and Compositions for Synthesis of Encoded Libraries
    Publication number: 20200149034
    Abstract: Methods of producing compounds and combinatorial compound libraries, the compounds and libraries produced via the methods are provided, and methods of using the libraries to identify compounds having a desired property, such as a desired biological activity and the compounds identified using these methods are provided.
    Type: Application
    Filed: March 16, 2018
    Publication date: May 14, 2020
    Inventors: Jin Li, Barry A. Morgan, Jinqiao Wan, Dengfeng Dou, Guansai Liu, Yong Chang, Qiuxia Chen, Xing Wang, Yanshan Xu, Dongyan Hu, Jian Liu, Xuemin Cheng
  • AUTOMATED SYSTEM FOR HTP GENOMIC ENGINEERING
    Publication number: 20200149035
    Abstract: The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.
    Type: Application
    Filed: November 27, 2019
    Publication date: May 14, 2020
    Inventors: Zach Serber, Erik Jedediah Dean, Shawn Manchester, Katherine Gora, Michael Flashman, Erin Shellman, Aaron Kimball, Shawn Szyjka, Barbara Frewen, Thomas Treynor, Kenneth S. Bruno
  • METHODS TO DISTINGUISH RNA AND DNA IN A COMBINED PREPARATION
    Publication number: 20200149036
    Abstract: The invention provides a series of steps to distinguish RNA from DNA in assays downstream of a combined isolation. This enables a wide diversity of RNAs and/or DNAs, to be efficiently detected.
    Type: Application
    Filed: January 2, 2018
    Publication date: May 14, 2020
    Inventors: Daniel ENDERLE, Johan SKOG, Phillipp TORKLER, Alexandra SPIEL, Mikkel NOERHOLM
  • WHOLE TRANSCRIPTOME ANALYSIS OF SINGLE CELLS USING RANDOM PRIMING
    Publication number: 20200149037
    Abstract: Disclosed herein include systems, methods, compositions, and kits for whole transcriptome analysis (WTA) with random priming and extension (RPE). The RPE-based WTA method can comprise hybridizing random primers with a plurality of first strand barcoded polynucleotides associated with a solid support and extending the random primers to generate a plurality of extension products. The method can comprise amplifying the plurality of extension products to generate a sequencing library.
    Type: Application
    Filed: November 7, 2019
    Publication date: May 14, 2020
    Inventor: Eleen Shum
  • METHODS AND COMPOSITIONS RELATING TO HOMOLOGY-DIRECTED REPAIR
    Publication number: 20200149038
    Abstract: The methods and compositions described herein relate to improvements in the efficiency and/or accuracy of targeted alterations to a nucleic acid sequence, e.g, gene editing technologies, by creating nick or DSB in a target nucleic in the presence of template molecule, an inhibitor of NHEJ and an agonist of HDR. In contrast to earlier technologies, these methods are not specific to each template and/or target sequence while retaining specificity of the editing itself.
    Type: Application
    Filed: March 28, 2017
    Publication date: May 14, 2020
    Applicant: CHILDREN'S MEDICAL CENTER CORPORATION
    Inventors: Derrick J. ROSSI, Bruna PAULSEN, Pankaj K. MANDAL, Wataru EBINA, Paula GUTIERREZ-MARTINEZ
  • REGULATION OF TRANSCRIPTION THROUGH CTCF LOOP ANCHORS
    Publication number: 20200149039
    Abstract: Disclosed are methods of altering expression of a gene with a promoter region CTCF binding site. Also disclosed are compositions and methods useful for treating a disease or condition involving over-expression or under-expression of a gene with a promoter region CTCF binding site. Further disclosed are cells and non-human animals with modified a promoter region CTCF binding site, as well as methods for screening for compounds that can modify the expression of a gene with a promoter region CTCF binding site.
    Type: Application
    Filed: December 12, 2017
    Publication date: May 14, 2020
    Inventors: Jurian Schuijers, Abraham S. Weintraub, John C. Manteiga, Richard A. Young
  • ANTISENSE NUCLEIC ACIDS
    Publication number: 20200149040
    Abstract: Provided is a drug that allows highly-efficient skipping of exon 51 in the human dystrophin gene. The present invention provides an antisense oligomer which enables exon 51 in the human dystrophin gene to be skipped.
    Type: Application
    Filed: June 11, 2019
    Publication date: May 14, 2020
    Applicants: NIPPON SHINYAKU CO., LTD., NATIONAL CENTER OF NEUROLOGY AND PSYCHIATRY
    Inventors: Tatsushi WAKAYAMA, Haruna SEO, Youhei SATOU, Shin'ichi TAKEDA, Tetsuya NAGATA
  • Gene Therapy Drug for Granular Corneal Degeneration
    Publication number: 20200149041
    Abstract: The present invention relates to a guide RNA molecule that hybridizes with a target sequence containing a mutation site of a transforming growth factor ?-induced (TGFBI) gene associated with granular corneal degeneration.
    Type: Application
    Filed: June 7, 2018
    Publication date: May 14, 2020
    Applicants: The University of Tokyo, National University Corporation Chiba University
    Inventors: Tomohiko Usui, Yukako Taketani, Yasuo Ouchi
  • MODULATING BIOMARKERS TO INCREASE TUMOR IMMUNITY AND IMPROVE THE EFFICACY OF CANCER IMMUNOTHERAPY
    Publication number: 20200149042
    Abstract: The present invention relates, in part, to methods of treating a cancer in a subject comprising administering to the subject a therapeutically effective amount of an agent that inhibits one or more biomarkers listed in Table 1, such as regulators of ubiquitin proteasome pathway (e.g., STIP1 homology and U-Box containing protein 1 (STUB 1), ubiquilin-1 (UBQLN1), and heat shock protein 90 kDa beta member 1 (HSP90B 1)), in combination with an immunotherapy.
    Type: Application
    Filed: July 16, 2018
    Publication date: May 14, 2020
    Inventors: William N. Haining, Robert Manguso
  • RNA MODULATING OLIGONUCLEOTIDES WITH IMPROVED CHARACTERISTICS FOR THE TREATMENT OF NEUROMUSCULAR DISORDERS
    Publication number: 20200149043
    Abstract: The current invention provides an improved oligonucleotide and its use for treating, ameliorating, preventing, delaying and/or treating a human cis-element repeat instability associated genetic neuromuscular or neurodegenerative disorder.
    Type: Application
    Filed: October 22, 2019
    Publication date: May 14, 2020
    Inventors: Peter Christian DE VISSER, Susan Allegonda Maria MULDERS
  • CONTROL OF INSECT INFESTATION
    Publication number: 20200149044
    Abstract: Provided herein are methods for using RNAi molecules targeting a proteasome beta 5 (PSMB5) gene for controlling Coleopteran insects, methods for producing RNAi molecules targeting PSMB5, and compositions comprising RNAi molecules targeting PSMB5.
    Type: Application
    Filed: November 8, 2019
    Publication date: May 14, 2020
    Applicant: GreenLight Biosciences, Inc.
    Inventors: Thais Barros Rodrigues, Suresh Desai, Krishnakumar Sridharan
  • MODULATORY POLYNUCLEOTIDES
    Publication number: 20200149045
    Abstract: The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of modulatory polynucleotides.
    Type: Application
    Filed: January 22, 2020
    Publication date: May 14, 2020
    Inventors: Dinah Wen-Yee Sah, Jinzhao Hou, Mathieu E. Nonnenmacher