Abstract: A method of an in-situ calibration of a chemiresistor sensor is disclosed. The method comprising: heating and/or cooling at least one chemiresistor sensor in a ?T° C., wherein the at least one chemiresistor sensor is assembled in a sensing device; receiving a first temperature signal indicative of temperature measurement from a temperature sensor located in the sensing device to the chemiresistor sensor; receiving a first signal from the chemiresistor sensor, and determining a mathematical correlation between the first temperature signal and the first signal. In some embodiments, the heating and/or cooling induces changes in the signal indicative of temperature measurement and the signal received from the chemiresistor sensor.

Abstract: According to one embodiment, a sensor includes a base and an element section. The element section includes a fixed electrode, a first support structure, and a movable member. The first support structure includes, a first fixed member, a first intermediate member supported by the first fixed member, a first connecting member, a first support member, a first cross fixed member, a first cross intermediate member, and a first cross connecting member. The first connecting member includes a first connecting portion, a first support connecting portion, and a first intermediate connecting portion provided between the first connecting portion and the first support connecting portion. The first cross connecting member is supported by the first cross intermediate member.

Abstract: According to one embodiment, a sensor includes a base including a first base region and a second base region, and an element section. The element section includes a fixed electrode fixed to the second base region, a first fixed member fixed to the first base region, a first intermediate member supported by the first fixed member, a first connecting member supported by the first intermediate member, a movable portion, and a first support member. The first connecting member includes a first connecting portion, a second connecting portion, and a third connecting portion. The movable portion is connected to the second connecting portion. The movable portion includes a movable electrode. A first gap is provided between the fixed electrode and the movable portion. The first support member is fixed to the base. The first support member supports the third connecting portion.

Abstract: A low power variable frequency soil sensor assembly is provided. The assembly is self contained and includes a housing from which a probe extends. A microcontroller generates a variable frequency signal which propagates via electrodes on the probe, generating an electric field into the surrounding soil. A capacitive sensor then can measure the soil moisture, conductivity, and nutrient level. The resulting measurement is read and wirelessly transmitted via the microcontroller to cloud systems for further intelligence and analytics. The assembly operates on very low regulated voltage which allows more stable operation over time, and reduces the frequency of battery replacement.

Abstract: The present invention provides an electrochemical test strip (200) for detecting multiple indicators and a test method thereof. The electrochemical test strip includes a first biosensor (100) provided with an insulating substrate (1), a first electrode system, a first channel forming layer (4) and a first upper cover layer (5), and a second biosensor (101) provided with an insulating substrate (8), a second electrode system, a second channel forming layer (11) and a second upper cover layer (12). The first biosensor (100) and the second biosensor (101) are located on the front and back sides of the electrochemical test strip (200), respectively. A sample addition port (6) of the electrochemical test strip (200) is in liquid communication with a first channel forming area (55) of the first biosensor (100) and a second channel forming area (56) of the second biosensor (101), respectively.

Abstract: An electro-chemical sensor based on 4-nitrothiophenol (4-NTP) functionalized heterogeneous layers of gold/graphene/Cu for highly sensitive detection of sugars, alcohols, and/or organic compounds in a sample is developed. 4-NTP molecules were immobilized into the surface of gold nanostructures. Due to the adsorption of 4-NTP on the surface, the new sensors showed more sensitivity and selectivity than conventional sensors.

Abstract: An electro-chemical sensor based on 4-nitrothiophenol (4-NTP) functionalized heterogeneous layers of gold/graphene/Cu for highly sensitive detection of sugars, alcohols, and/or organic compounds in a sample is developed. 4-NTP molecules were immobilized into the surface of gold nanostructures. Due to the adsorption of 4-NTP on the surface, the new sensors showed more sensitivity and selectivity than conventional sensors.

Abstract: An electro-chemical sensor based on 4-nitrothiophenol (4-NTP) functionalized heterogeneous layers of gold/graphene/Cu for highly sensitive detection of sugars, alcohols, and/or organic compounds in a sample is developed. 4-NTP molecules were immobilized into the surface of gold nanostructures. Due to the adsorption of 4-NTP on the surface, the new sensors showed more sensitivity and selectivity than conventional sensors.

Abstract: An electro-chemical sensor based on 4-nitrothiophenol (4-NTP) functionalized heterogeneous layers of gold/graphene/Cu for highly sensitive detection of sugars, alcohols, and/or organic compounds in a sample is developed. 4-NTP molecules were immobilized into the surface of gold nanostructures. Due to the adsorption of 4-NTP on the surface, the new sensors showed more sensitivity and selectivity than conventional sensors.

Abstract: The strip systems, methods, devices and associated kits disclosed herein are used to determine the presence and/or level a target analyte(s) in sample (e.g., a biological sample such as saliva or nasal swab) wherein the target analyte(s) may be a microorganism (e.g., a whole virus) or molecule (e.g., a viral antigen) associated with a healthy state, disease or injury or otherwise altered physiological condition. In certain embodiments, the systems, methods, devices and kits provide one or more improved properties relative to the lateral flow protein and other assays known in the art for the detection, including but not limited to, assay time, ease of use, risk of infection, accuracy, specificity, selectivity, limit of detection of the assay, quantitative detection and the effect of common interferents to the sensor output, cost, simplicity or a combination thereof. In certain embodiments, the systems, assays, methods and kids are multiplexed, i.e.

Abstract: An aptamer sensing device is provided. The aptamer sensing device 200 includes at least one electrode 220. The aptamer sensing device further includes at least one binding feature 230. The aptamer sensing device further includes a plurality of aptamers 270 attached to the binding feature and not individually attached to the electrode, the aptamers further having an attached redox couple 272. The aptamer sensing device is configured to accept a sample fluid 242 including at least one analyte, and the binding feature is positioned relative to the electrode such that a binding of the analyte to the aptamers causes a shape change configuration in the binding feature that increases or decreases a charge transfer from the redox couple to the electrode.

Abstract: A probe includes a fast-scanning cyclic voltammetry electrode, a conductive wall disposed around the fast-scanning cyclic voltammetry electrode, and a wire in electronic communication with the fast-scanning cyclic voltammetry electrode. The conductive wall is grounded. Fast scanning cyclic voltammetry electrode currents are enclosed within the conductive wall. Resulting fast scanning cyclic voltammetry can have sub-?V level artifacts.

Abstract: An electrochemical device includes a housing, a first member and a second member provided between the housing and the first member. The housing has a first through hole including a first opening that faces the first member. The second member has a second through hole including a second opening.

Abstract: A gas sensor control device controls a gas sensor including a first pumping cell and a second pumping cell configured to output a current in accordance with a concentration of a specific component in a measurement target gas. The gas sensor control device has a microprocessor configured to, when calculating the concentration of the specific component, use a correction expression based on an index including at least either an initial offset indicating a current value when the concentration of the specific component at a first reference time is 0 or a difference between an ideal value and the current value when the concentration of the specific component at a second reference time is a set concentration, and a cumulative operating time which is a total operating time of the gas sensor from a third reference time.

Abstract: Circuitry for processing a response from an electrochemical cell to a stimulus, the circuitry comprising: sense circuitry configured to measure the response of the electrochemical cell to the stimulus; and processing circuitry configured to: sample the measured response to obtain a plurality of samples; and output the plurality of samples, wherein the processing circuitry is operable in: a continuous sampling mode in which the processing circuitry is configured to periodically sample the measured response at a fixed sampling rate; a discontinuous sampling mode in which the processing circuitry is configured to periodically sample the measured response during macro periods separated by macro intervals during which sampling of the measured response is disabled.

Abstract: Systems, methods, and devices for imaging of stain-free fluorescence on western blot membranes with excitation by epi illumination with UV LEDs are disclosed herein. A method can include collecting and preparing a sample, separating the sample via gel electrophoresis in a gel block, transferring the separated sample from the gel block to an analysis block, generating an image of the sample with an imager, and evaluating the image of the sample. The imager can include a plane to receive and hold a block containing a sample and including a first side and a second side, a camera to image a sample on the plane and positioned above the first side of the plane, and an LED light source positioned above the first side of the plane. The LED light source can illuminate the sample on the plane via epi-illumination, and emits light having a wavelength in a range from approximately 325 nm to approximately 400 nm.

Abstract: Non-destructive testing of an elongate object can include using an adaptable apparatus to support objects having different cross-sectional profiles. For example, an adaptable inspection fixture can support inspection probes for acoustic or eddy current inspection, as illustrative examples. Generally, the apparatus comprises opposing portions which are pivotable and connected by a linkage to maintain the opposing portions in a specified orientation relative to each other while permitting independent rotational orientations of each respective opposing portion such as to accommodate test objects. For example, the opposing portions can be substantially parallel across multiple configurations. Optionally, the apparatus can maintain a specified orientation of one or more radius probes with respect to an object under test.

Abstract: A non-invasive liquid detection method based on acoustic wave features and an apparatus thereof, the method includes: collecting acoustic waves penetrating a liquid to be tested and extracting acoustic absorption-transmission curve features from the acoustic waves to generate a liquid fingerprint, the acoustic absorption-transmission curve features represent a ratio of an energy of an acoustic signal penetrating the liquid to an energy of an acoustic signal emitted across a plurality of frequencies; inputting the liquid fingerprint into a trained neural network model for detection processing, and outputting detection results. The neural network model is trained with a plurality of sets of data, and each set of data includes: acoustic absorption-transmission curve features, and a label identifying a feature that meets a detection requirement in the acoustic absorption-transmission curve features.

Abstract: An integrated circuit (IC) includes a semiconductor substrate and a mold compound on the semiconductor substrate. The IC also includes an acoustic signal generator between the mold compound and the semiconductor substrate. The acoustic signal generator is configured to transmit an acoustic signal having a predetermined set of frequencies through at least one of the semiconductor substrate or the mold compound.

Abstract: A degradation judgement system includes a field repair unit that is provided in a banknote handling apparatus, a vibration detection sensor, a judgement unit, and a notification unit, wherein the field repair unit includes a rubber part, the vibration detection sensor measures vibration data by detecting vibration of the field repair unit, the judgement unit judges a degree of deterioration of the rubber part included in the field repair unit based on a peak of a vibration amplitude that is obtained from the vibration data and based on a root mean square of the vibration amplitude, and the notification unit gives a notification of the judged degree of deterioration.

Abstract: The present invention is directed to nondestructive testing of objects, and more specifically to a system for nondestructive testing of an object using a robotic arm. The end effector of the robotic arm is equipped with an arrangement of paired, angled transducers and an orthogonal transducer configured to scan an object using multiple wavelengths of ultrasonic sound waves. Multiple scans of a test object are taken as the object is rotated and the scans are then wirelessly transmitted to a user device or a remote server.

Abstract: A method of calibrating an ultrasound apparatus for use in scanning an object to obtain subsurface information from the object, the ultrasound apparatus comprising an ultrasound transceiver for transmitting an ultrasound pulse towards the object and receiving a resultant ultrasound reflection signal from the object; the method comprising: determining a characteristic material in respect of the object to be scanned; selecting a feature of the characteristic material against which to calibrate the ultrasound apparatus; driving a pre-defined ultrasound pulse template into the characteristic material; receiving a resultant ultrasound reflection signal from the characteristic material; gating the received resultant ultrasound reflection signal based on the selected feature; and calibrating the ultrasound apparatus using the gated received resultant ultrasound reflection signal.

Abstract: Disclosed is a measuring device for measuring the height of a foreign object in a pipe comprising an ultrasound transmission unit including an ultrasound generator disposed in a first area of an outer surface of a pipe through which fluid passes, and generating ultrasound, and a transmission medium disposed between the ultrasound generator and the first area to transmit the ultrasound to the first area, an ultrasound receiving unit including a receiving medium unit disposed in the second area of the outer surface of the pipe, and consisting of an ultrasound receiver, and a receiving medium disposed between the ultrasound receiver and the second area, and a signal measurer connected to the ultrasound receiver for measuring the ultrasound signal received by the ultrasound receiver or a signal corresponding thereto.

Abstract: A diagnosis system and a diagnosis method are presented, which utilize a CNN-based signal analysis, that are capable of diagnosing various mechanical equipment pieces by analyzing vibration or noise occurring in the various mechanical equipment pieces in industrial sites on the basis of a deep learning technique. The effect of efficiently performing signal analysis and signal-feature extraction can be achieved. The effect of extracting a feature of an automatically measured signal on the basis of a result obtained through a feature extraction process in a case where it is necessary to set up a database can be achieved.

Abstract: A system comprises a separator that separates an analyte from the sample, an analyser, downstream of the separator, configured to obtain measurements indicative of a quantity of the analyte in the sample. A bypass line provides a first fraction of the sample to the analyser for measurement without passing through the separator. A controller receives a measurement obtained by the analyser on the first fraction of the sample, the measurement indicating that the first fraction of the sample received via the bypass line comprises a threshold quantity of the analyte. Activation of the separator is based on the received measurement. A second fraction of the sample is provided to the analyser without passing through the bypass line such that the first fraction of the sample arrives at the analyser before the second fraction of the sample arrives at the separator.

Abstract: The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. The method includes introducing a sample comprising the analytes into a chromatographic system. The chromatographic system has a flow path disposed in an interior of the chromatographic system, at least a portion of the flow path having an active coating, and a chromatographic column having a stationary phase material in an interior of the chromatographic column that facilitates separation of the analytes in the sample through interaction with at least one analyte in the sample. The active coating is selected to interact with at least one analyte in the sample through (1) a repulsive force, (2) a secondary interaction, or (3) a retention mechanism distinct from the interaction with the stationary phase material.

Abstract: The present disclosure is directed to methods of separating one or more non-modified oligosaccharide(s) with degree of polymerization 6 or higher from a sample using a hydrophilic interaction liquid chromatography (HILIC) column connected to a liquid chromatography system that includes coated flow paths. The methods disclosed herein requires, inter alia, a liquid chromatography system, wherein at least one component of the LC system includes a fluid-contacting alkylsilyl coating. When implementing a standard HILIC method, the methods of the present technology can be used to decrease the analyte loss and/or carry-over of the separation leading to improved analysis.

Abstract: Threaded conventional column end fittings for liquid chromatography columns do not have face seals that enable them to be used with clamping fluidic connectors. The exemplary embodiments may provide adapters that interface with conventional threaded column end fittings so that the column assembly may now be used with the clamping fluidic connectors. The adapters of the exemplary embodiments may include lock nuts for securing the adapters to column end fittings of a liquid chromatography columns. The lock nuts may include threads that bear the load of the adapter rather than the column end fitting of the chromatography column. The threads of the lock nut become the load bearing surfaces, and the load is distributed over the surfaces where the threads of the adapter engage with the threads of the lock nut.

Abstract: The present invention relates to a method for detecting glycosidic bonds of plant polysaccharides. The method includes taking 100 ?L of NaOH-DMSO suspension (1 mg/mL) of plant polysaccharides containing 20 or more kinds of substances such as galactan, araban, polygalacturonic acid, rhamnogalacturonan-I, rhamnogalacturonan-II, xyluronic acid, arabogalacturonic acid I and arabogalacturonic acid II, shaking at room temperature for 30 minutes, adding 40 ?L of iodomethane and shaking for 1 hour. Such process is repeated three times to obtain methylated plant polysaccharides. The method further includes using an automatic detection equipment for methylation combined with PMP derivatization to analyze glycosidic bonds in polysaccharides. Not only the glycosidic bond information of neutral sugars can be analyzed in the subsequent LC-MS mass spectrometry analysis, but also the glycosidic bond information of acidic sugars can be resulted, and the analysis results are faster, more detailed and more reliable.

Abstract: A method, mass spectrometer and computer readable medium for acquiring mass spectral data; comprising acquiring mass spectral data for a sample in a profile mode; computing spectral loadings from the acquired mass spectral data; operating with the spectral loadings and a mass spectral library spectrum in profile mode to calculate a search score for a library compound; and reporting search scores indicating the likelihood of library compounds being present in the sample. A regression analysis is performed to estimate the relative concentrations of possible compounds. The method can be implemented in the form of a server located amongst networks, such as the worldwide web, computers, various devices, and MS instruments. Users of the method, while waiting for results, are exposed to advertising. The advertising is selected to be relevant to the compounds being analyzed. Users of the method are provided with a subscription to updates in the library.

Abstract: The present invention concerns a HPLC separation method useful in the synthesis of [18F]-labelled compounds, including positron emission tomography (PET) tracers. The method of the invention addresses constraints of previous methods imposed by the needs of free 5 [18F]fluoride. The present invention provides a simplified process that enables rapid separation and analysis of free [18F]fluoride and chemical impurities in the synthesis of [18F]-labelled compounds.

Abstract: Provided herein are assays utilizing oxygen-sensitive fluorescent materials for the detection of oxygen. In particular, oxygen-sensitive fluorescent particles are provided for monitoring the oxygen consumption rate and metabolic fitness of living cells.

Abstract: Disclosed herein are gas detector devices and methods of making and use thereof. The gas detector devices comprise: a temperature control layer; a grounded electrode; and a pyroelectric layer; the grounded electrode being disposed between the temperature control layer and the pyroelectric layer; and a detection electrode opposite and spaced apart from the pyroelectric layer defining an ionization zone therebetween. Disclosed herein are gas detection methods comprising: introducing a gas into the ionization zone; heating/cooling the temperature control layer to induce a first potential in the pyroelectric layer sufficient to ionize a first gas component, thereby producing a first ion; detecting the first ion; subsequently, heating/cooling the temperature control layer to induce a second potential in the pyroelectric layer sufficient to ionize the first gas component and a second gas component, thereby producing the first ion and a second ion; and electrically detecting the first ion and the second ion.

Abstract: A broad hybrid forest regression (BHFR)-based soft sensor method for DXN emission in a municipal solid waste incineration (MSWI) process, including: based on a broad learning system (BLS) framework, constructing a BHFR soft sensor model for small sample high-dimensional data by replacing a neuron with a non-differential base learner, where the BHFR soft sensor model includes a feature mapping layer, a latent feature extraction layer, a feature incremental layer and an incremental learning layer, and the method includes: mapping a high-dimensional feature; extracting a latent feature from a feature space of a fully connected hybrid matrix, and reducing model complexity and computation consumption based on an information measurement criterion; enhancing a feature representation capacity by training the feature incremental layer based on an extracted latent feature; and constructing the incremental learning layer based on an incremental learning strategy, obtaining a weight matrix with a Moore-Penrose pseudo-inv

Abstract: A toxic material detecting device includes an outer casing and a toxic material retaining composition within the casing. The toxic material detecting device can detect the presence of radioactive, chemical, biological, and toxic materials in air, gases, oils, soil, and water that contact one or more detection chambers within the device. The toxic material retaining composition can retain the toxic material within the device for a period of time sufficient for detection, thereby providing increased sensitivity for the device.

Abstract: An apparatus for gathering soil data along a trajectory comprises a crawler body that houses a processor that is configured with instructions for controlling the apparatus, memory coupled to the processor, and a GPS module and motor also coupled to the processor. The apparatus also includes a pair of continuous tracks coupled to the crawler body and rotatable through a coupling with the motor, wherein the continuous tracks include planar sections, one or more of the planar sections including a plurality of flexible smart sensors that are each configured to detect and store data concerning at least one property or condition of the soil when the apparatus moves over the soil along the trajectory. The flexible smart sensors are resilient when subjected to bending forces when the continuous tracks move along the soil.

Abstract: A method of processing a core sample includes the steps of: hermetically sealing the core sample in a chamber of an enclosure, comminuting the core sample while the chamber is hermetically sealed, injecting a hydrophilic agent into the chamber, wherein a slurry is formed by at least the hydrophilic agent and water released by the core sample, extracting at least a portion of the slurry from the chamber, separating a fluid from the slurry, analyzing the separated fluid to estimate an amount of water in the fluid, and estimating a water content of the core sample using the estimated amount of water in the fluid.

Abstract: A chip agitation system for use on a rotorcraft with an engine and transmission system. The chip agitation system is configured to produce a mass flux of fluid to agitate chip debris towards a chip detection device. The chip agitation system includes a reservoir and valve that can be selectively opened and closed.

Abstract: A blood analyzer for analyzing multiple blood parameters is disclosed. The blood analyzer comprises an inlet module configured to receive an initial blood sample, a blood gas sensor device in fluid communication with the inlet module, the blood gas sensor device configured to receive a first blood sample of the initial blood sample and conduct a blood gas analysis on the first blood sample to determine a blood gas analysis parameter, a blood count sensor device in fluid communication with the inlet module, the blood count sensor device configured to receive a second blood sample of the initial blood sample and determine a blood count parameter, and an output configured to provide the blood gas analysis parameter and the blood count parameter.

Abstract: The invention relates to biomarkers, and to novel biological markers for diagnosing colorectal cancer. In particular, the invention relates to the use of these biomarkers as diagnostic and prognostic markers in assays for detecting colorectal cancer, and corresponding methods of detection. The invention also relates to methods of determining the efficacy of treating colorectal cancer with a therapeutic agent, and apparatus for carrying out the assays and methods. The assays are qualitative and/or quantitative, and are adaptable to large-scale screening and clinical trials.

Abstract: The present invention relates to a method for predicting how a cancer patient will respond to an antibody drug conjugate (ADC) therapy involving computing a predictive response score based on single-cell ADC scores for each cancer cell. For each cancer cell, a single-cell ADC score is computed based on the staining intensities of the dye in the membrane and cytoplasm of the cancer cell and in the membranes and cytoplasms of neighboring cancer cells. The present invention also relates to predicting a response of a cancer patient to ADC therapy by aggregating all single-cell ADC scores of the tissue sample using a statistical operation, and the subsequent treatment of cancer with related antibody-drug conjugates.

Abstract: Provided are methods and articles of manufacture for use with cell therapy for the treatment of diseases or conditions, e.g., cancer, including for predicting likelihood of the subject responding to a therapy, such as a cell therapy, e.g., a chimeric antigen receptor (CAR) T cell therapy. In some aspects, the predicting is based on detecting certain biomarkers of immune cells associated with and/or that correlate with response following administration of the therapy. The methods generally involve detecting a marker by assaying a biological sample from a subject that is a candidate for treatment, optionally with a cell therapy, to determine if the subject is likely to respond to the therapy. The present disclosure also provides methods for treating a subject having a disease or condition, in some cases involving administration of the cell therapy, based on assessment the biomarker. Also provided herein are reagents and kits for performing the methods.

Abstract: Provided herein are methods of identifying an inhibitory activity of an exogeneous agent that disrupts binding of a target peptide to a biomolecule using phase separation as in cellulo read-out. Provided herein also are kits for the methods, pharmaceutical compositions comprising the agents screened by the methods, methods of preparing the pharmaceutical composition, and methods of treating a disease or condition in a subject in need thereof by administering the pharmaceutical composition.

Abstract: Provided herein is a recombinant cell for drug candidate screening. Also provided herein is a method of producing the recombinant cell, and a method of identifying a drug candidate comprising providing the recombinant cell.

Abstract: A method of rapid constructing human cerebral cortical organoids by 3D bioprinting and an application including preparing microfluidic chips, preparation of hydrogel of human cerebral cortical organoids, and printing of human cerebral cortical organoids. The microfluidic chip comprises a mixed-flow channel layer, liquid pool layer, microporous array layer, human cerebral cortical organoid culture layer, and culture medium recovery layer; the human cerebral cortical organoid hydrogel has gelatin, alginate, and hyaluronic acid; printing directly human cerebral cortical organoids in microfluidic chips by FRESH printing method, obtaining human cerebral cortical organoid chips after packaging. The application directly constructs large-scale human cerebral cortex-like with three layers of mutually connected structures in situ in organ chip through 3D bioprinting, simulates cerebrospinal fluid circulation through perfusion culture.

Abstract: An object of the present invention is to provide a cell culture device capable of reproducing, in vitro, a growth and proliferation environment of an anaerobic bacterium in a living body; a kit for cell culture; and a cell culture method. According to the present invention, there is provided a cell culture device including a plate having a concave portion for accommodating a culture medium, a cell accommodating container having a gas permeable membrane, a first member for holding the cell accommodating container, and a second member having a structure for regulating a gas concentration in an upper space of the cell accommodating container, in which, in a case where a cell sheet is formed on an upper surface of the gas permeable membrane, a space above the gas permeable membrane is in a sealed state in which gas does not pass through.

Abstract: A micro-nano sensing device for sensing a chemical, biological, or biochemical entity includes a non-conductive housing having a surface and that contains supporting logic circuitry, signaling circuitry electrically connected to the logic circuitry, and at least one electrically conductive pad positioned on the housing surface and electrically connected to the logic circuitry and to a targeting agent capable of interacting with a target.

Abstract: The current technology is related to methods for rapidly determining the metabolic baseline and potential of living cells. Embodiments relate to measuring the activity of each of the two major energy-generating pathways within the cell: mitochondrial respiration and glycolysis, first under baseline conditions, and again after applying a stress to the cells to demand increased energy supply. In some embodiments the stress may be applied by exposing the cells to a combination of two chemical compounds: a mitochondrial uncoupler and an ATP synthase inhibitor. In one embodiment, the metabolic energy generating activity of the mitochondrial respiration pathway is determined by measuring the rate of oxygen consumption by the living cells, and the metabolic energy generating activity of the glycolysis pathway is determined from a measurement of extracellular acidification caused by secretion of protons from the cell.

Abstract: The present invention relates to the field of screening methods. More specifically, the present invention pertains to a method for determining a toxicity and/or growth promotion effect of any one of a treatment or compound, and a computer program comprising instructions to carry out steps of said method.

Abstract: System and method embodiments for analyzing a test fluid to detect prior or present infestations of bed bugs are described. In an embodiment, the method may include receiving the test fluid on a test strip within the detection device. The test strip may include a reaction portion and a reagent portion containing an antibody or antigen-binding fragment that is conjugated to a colored particle. The test fluid may include bed bug antigen that reacts with the conjugated antibody. The detection device may include a first and a second optical sensor for monitoring a reaction and a background color intensity, respectively. Upon a predetermined time delay elapsing, the detection device determines whether bed bug antigen is present in the test fluid using the monitored color intensities and minimum and maximum color intensity thresholds associated with bed bugs. Then, the detection device outputs a result using a visual display.