Abstract: A method of detecting the expression level of miRNA markers in a biological sample obtained from a mammal is provided. The method incudes the steps of i) detecting the expression level of one or more miRNA markers selected from the group of miR-199a-3p, miR-143-3p, miR-340-5p, let-7b-5p, miR-21-5p, miR-17-5p, miR-20a-5p and miR-103a-3p, in the biological sample; ii) detecting the expression level of at least one miRNA reference marker selected from miR-148b-3p and miR-30e-5p in the biological sample; and iii) normalizing the expression level of the miRNA marker(s) against the expression level of the miRNA reference marker in the sample and in a control. The method is useful for the diagnosis of endometriosis, monitoring of patient response to treatment, and assessment of disease progression and/or severity.

Abstract: The present invention relates to assays, methods, and kits to assess traumatic brain injury (TBI) in a subject. The present invention also relates to ultrasensitive assays for GFAP, tau, CKBB, IL-1?, IL-2, IL-6, IL-10, IL-22, IP-10, TNF?, TSLP, NFL, and/or NFH.

Abstract: A compensation bead comprising an amine functionalized bead modified covalently or non-covalently with a carboxyaryl porphyrin, a method for making the amine functionalized bead with the carboxyaryl porphyrin and a method of using the amine functionalized bead with the carboxyaryl porphyrin as a marker for the carboxyaryl porphyrin labeled cells and particles.

Abstract: The present embodiments relate generally to a method and apparatus of capturing and releasing magnetically activated cells. Embodiments include a microfluidic device capable of capturing a large array of magnetically functionalized cells and releasing any one specific captured cell without releasing the rest of the cells. Methods and devices according to embodiments provide an improvement over current microfluidic cell sorting approaches due to the use of a simple mechanism, which simultaneously allows for high selectivity, and high throughput potential.

Abstract: A test substance detection device of an embodiment includes a substrate on which a catcher having a binding ability to a test substance is immobilized, an external force applying mechanism, an imaging device, and processing circuitry. The processing circuitry is configured to control the external force applying mechanism to apply an external force to a particle on the substrate, the particle having an ability to bind to the test substance and having undergone a reaction for binding to the test substance, to control the imaging device to image the particle on the substrate in a state in which the external force has been applied, to determine a moving range of the particle due to application of the external force on the basis of the image captured by the imaging unit, and to distinguish between (a) the particle binding to the catcher via the test substance and (b) the particle binding to the catcher without the test substance.

Abstract: Provided are a particle that enables a high-sensitivity specimen test by a fluorescence depolarization method, and a method of producing the same. Specifically, provided is a particle including a particle substrate, wherein the particle substrate includes: a polymer containing a styrene unit and an organic silane unit; and a europium complex, and wherein the particle substrate contains a siloxane bond on at least a surface of the particle substrate.

Abstract: Compositions comprising a polymer matrix or film having dispersed therein a plurality of glass bubbles with affinity groups bound thereto, and methods of detecting target analytes using the same.

Abstract: Provided is a method for providing information on chronic urticaria and, more specifically, the provided is a method for diagnosing chronic urticaria by measuring an allergen-specific IgE antibody for a vesicle derived from Acinetobacter bacteria; a diagnostic composition using the same; and the like.

Abstract: An object of the invention is to suppress non-specific reaction in an immunoreaction measurement method. An immunoassay method characterized by conducting immunoreaction in the presence of an anti-C3 antibody in a method for measuring an analyte in a sample immunologically is provided. Moreover, a method for suppressing non-specific reaction in a method for immunologically measuring an analyte in a sample which is characterized by conducting immunoreaction in the presence of an anti-C3 antibody is provided.

Abstract: This document relates to materials and methods for assessing and/or treating mammals (e.g., humans having autoimmune diseases). For example, materials and methods for determining if a mammal (e.g., a human having an autoimmune disease) has one or more antibodies that can be used to identify the mammal as having a lower risk of cancer or as having a higher risk of cancer are provided. Materials and methods for treating a mammal (e.g., a human) identified as having a higher cancer risk for cancer are also provided.

Abstract: Migration of human peripheral leukocytes in response to chemotactic factors during pregnancy. The methods include determining the likelihood of a pregnant subject undergoing delivery within about 7 days by obtaining peripheral leukocytes from the pregnant subject and determining a chemotactic activity of the leukocytes, wherein an increased likelihood of delivery within about 7 days is indicated when the chemotactic activity is at a level characteristic of a full term pregnancy. Chemotactic activity is assessed by measuring migration of peripheral leukocytes towards a chemoattractant which is a factor present in a sample comprising human fetal membrane obtained from a full term pregnant subject. Other biomarkers or F-actin polarization of peripheral leukocytes are also indicative of the likelihood of a pregnant subject undergoing delivery. Further methods disclosed include minimizing complications of preterm delivery by administration of an IL-1 antagonist when there is a likelihood of preterm delivery.

Abstract: The present invention provides a system and methods for the detection of peptides from and antibodies against coronaviruses, filoviruses, flaviviruses, or combination thereof, in a sample. A method for detecting the presence of a coronavirus in a sample may comprise: collecting a small sample of a biological fluid from the test subject, adding sample to a viral test cassette, initiating sample processing by the assay cassette to incubate the diluted sample and developing reagents with an antibody microarray contained within the cassette, and results of the test are read by visual examination and process the results to determine if the subject is infected by a coronavirus.

Abstract: Methods of utilizing sample lysis reagent compositions in the handling of samples containing viruses, such as (but not limited to) severe acute respiratory syndrome coronavirus 2 (SARS-COV-2), are disclosed. Kits containing the sample lysis reagent compositions are also disclosed.

Abstract: To detect a biological substance at high speed and high sensitivity by using a well array and particles. The biological substance detection method of the present invention includes a step of preparing a test liquid containing particles at a concentration of at least 5×107 particles/mL, a step of allowing the particles to trap the biological substance to form a trapped body, a step of sending the test liquid onto the well array to store a plurality of the particles including the trapped body in the wells while storing the number of the particles to be stored in one well to a minimum average storage number Nmin represented by the following formulas (1) and (2), and a step of detecting the color development of the well with an image pickup element.

Abstract: The present disclosure describes methods of determining a treatment protocol for and/or a prognosis for a subject suspected of or at risk of suffering from a neurologic disease or disorder, including such diseases and disorders that involve motor neuron function such as ALS. The methods comprise detecting the presence of a chitinase protein in a biological sample.

Abstract: Aspects of the present invention relate to a method of assessing disease in a subject comprising identifying at least one cellular phenotype characteristic of one or more cells in a sample of the subject, identifying at least one clinical characteristic of the subject, using the at least one cellular phenotype characteristic and the at least one clinical characteristic to assess a presence of oral lichenoid conditions (OLC) in the subject. In some embodiments, the OLC is oral lichen planus (OLP) and oral lichenoid lesions (OLL). In some embodiments, the at least one clinical characteristic is selected from the group consisting of: lesion involvement, lesion appearance, lesion area, lesion color and lesion location.

Abstract: The spectrographic detection of acute leukemia provides for the early detection of acute leukemia, distinguishing between normal blood and blood infected by either Acute Lymphoblastic Leukemia (ALL) or blood infected by Acute Myeloid Leukemia (AML). The spectrographic detection of acute leukemia also provides an alternative method for monitoring the progress of treatment for acute leukemia. The method uses fluorescence spectroscopy techniques, including fluorescence emission spectra (FES) of acetone extracts of red blood cells, synchronous fluorescence emission spectra (SFES) and synchronous fluorescence excitation spectra (SFXS) of different biomolecules found in blood plasma, and comparing ratios of the intensities obtained at certain wavelength peaks.

Abstract: The present disclosure relates to a device and uses thereof for detecting, treating, and monitoring cancers.

Abstract: The invention generally relates to the field of individualized medicine. It is a kind of liquid biopsy for individualizing and monitoring treatment in patients with tumors and for diagnosis of tumors. The invention relates to a method for identifying and characterizing subpopulations (subgroups) of circulating tumor cells (CTCs) in a population of CTCs in a biological sample, preferably in a blood sample of the patient. The invention also relates to the quantification of the respective subgroups of CTCs in the population of CTCs in the biological sample. The invention further relates to the use of the method for diagnosis and monitoring of tumors, decisions relating to treatment, surveillance of treatment and prognosis, in particular for all kinds of solid tumors. The invention further relates to the use of the identified CTC subgroups as biomarkers for diagnosis, prognosis and therapeutic treatment.

Abstract: The present invention provides methods of identifying neopeptides in abnormal cells, with the methods comprising sequencing long-read messenger RNA (mRNA) isolated from the abnormal cells, identifying splice variants that could be generated from the sequenced long-read mRNA, determining if the abnormal cells contain neopeptides that correlate with the identified splice variants. The methods may also comprise identifying at least one neoantigen on the neopeptides that are present in the abnormal cells.

Abstract: Metabolic engineering has developed microbial cell factories as sustainable alternatives to chemical synthesis from petroleum feedstocks or harvesting of animals and plants, but current methods can be a costly and labor-intensive commitment. Methods are described herein for microfluidic methods of screening thousands of variant cells in order to reduce time and uncertainty to provide improved strains.

Abstract: The invention relates generally to the field of biochemistry. In particular, the invention relates to a method of detecting or measuring target that is bound to a ligand in a sample, the method comprising contacting a sample comprising one or more cells with a cell-permeable denaturant to promote intracellular unfolding of the target in the sample, followed by lysing the sample. The lysed sample is then detected or measured for the level of non-aggregated target or aggregated target, wherein a difference in the level of non-aggregated target or aggregated target as compared to a reference indicates the presence or level of target that is bound to the ligand in the sample. In specific embodiments, the cell-permeable denaturant is urea or derivatives thereof. Methods of identifying a candidate ligand or predicting the efficacy of a drug in a subject are also provided therein.

Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides comprising unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.

Abstract: A method for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding is disclosed.

Abstract: A method for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding is disclosed.

Abstract: A peptide biomarker for identifying Hippocampus species based on peptidomics and application thereof are disclosed, belonging to the technical field of biology. The protein in Hippocampus is studied, and the Hippocampus thermostable protein is comprehensively analyzed using a peptidomics method. The analysis discovered potential peptide biomarkers of Hippocampus collagen, the specificity of the potential peptide biomarkers is verified by HPLC-triple quadrupole mass spectrometry, and the peptide biomarkers are comprehensively identified by combining bioinformatics analysis. Finally, 10 peptide biomarkers are discovered, targeting 11 different species of Hippocampus. The markers provided may identify Hippocampus existing in the market, promote the scientific and standardized classification and grading of Hippocampus commodities, and play an important role in high-quality and high-price trade circulation of the Hippocampus commodities.

Abstract: A metabolomic signature of acute mesenteric ischemia (AMI) and the determination thereof in a method for identifying a subject suffering or being at risk of suffering from AMI. Also, a kit that includes elements for determining the metabolomic signature of AMI and implementing the method for identifying a subject suffering or being at risk of suffering from AMI.

Abstract: Provided are a novel biomarker that can conveniently detect a tubulointerstitial disorder or that can grasp the disease state of a kidney disease using a urine sample, and a use thereof. The biomarker is scd14-st in urine.

Abstract: The present invention is directed to an immunoassay method for detecting an analyte in a liquid sample; and provides accuracy and reproducibility for both high concentration and low concentration samples. The method uses both fluorescence marker and chemiluminescence marker, and reads both fluorescence signal and chemiluminescence signal. The analyte concentration is determined based on either a calibration curve for the fluorescence signal, or a calibration curve chemiluminescence signal, using a pre-established analyte concentration value.

Abstract: The invention provides compounds of Formula I useful for the measurement of temperature by 19F magnetic resonance imaging.

Abstract: A method for determining whether a subject is at risk of dying from breast cancer or prostate cancer is disclosed.

Abstract: Methods, systems and circuits evaluate a subject's CVD risk using a risk parameter that includes at least one HDL and inflammatory biomarker interaction parameter. The inflammatory biomarker may optionally comprise NMR derived measurements of GlycA from at least one biosample of the subject. The risk parameter may be gender-specific.

Abstract: Methods, systems, and apparatus, including computer programs encoded on a computer storage medium, for detecting hazardous materials are disclosed. In one aspect, a method includes the actions of receiving, from one or more sensors, sensor data that reflects characteristics of an environment in a vicinity of the one or more sensors. The actions further include analyzing the sensor data. The actions further include determining that a hazardous material is in the vicinity of the one or more sensors. The actions further include selecting a collection device that is configured to store the hazardous material. The actions further include generating a control instruction that instructs the collection device to collect and store the hazardous material. The actions further include providing, for output to the collection device, the control instruction that instructs the collection device to collect and store the hazardous material.

Abstract: Disclosed is a specimen analyzer comprising: a rack transport part configured to transport a rack accommodating a plurality of specimen containers; an agitating part configured to agitate a specimen in each of the specimen containers; a suction part configured to suction the specimen from the specimen container; a container transport part configured to transport the specimen container between the rack on the rack transport part, the agitating part, and a suction position for specimen suctioning by the suction part; a measurement part configured to measure a measurement sample prepared from the specimen suctioned by the suction part; and a controller configured to control at least the agitating part, the suction part, and the container transport part, wherein the specimen analyzer is configured such that control by the controller causes, during a period from start to end of agitation of one of the specimen containers, at least one of transferring to the suction position, suctioning of a specimen, and returning

Abstract: A sample analysis system is available that can include a remote sampling system, at least one analyzer, and a controller. The remote sampling system can include a plurality of sample sources for providing a corresponding sample therefrom; and a plurality of sample collection devices selectively coupled to any of the plurality of sample sources for receiving at least one of the samples therefrom. The at least one analyzer can be coupled to the plurality of the sample collection devices for receiving at least one of the samples therefrom. The controller can be coupled with the remote sampling system and the at least one analyzer, the controller configured to control which of the sample sources is actively coupled to a given sample collection device at a given time.

Abstract: A method for operating a laboratory automation system, the laboratory automation system comprising a carrier comprising a reception place for receiving a sample container configured to contain a sample to be analyzed by a laboratory device; a placement device configured to pick and place the sample container; an imaging device; and a data processing device comprising at least one processor and a memory. The method comprises detecting an image of the reception place; determining whether the reception place is free for receiving the sample container and the reception place is configured to receive the sample container, by applying a machine learning algorithm for image analysis of the image of the reception place; and placing the sample container in the reception place by the placement device if the reception place is determined as free and configured to receive the sample container. Further, a laboratory automation system is disclosed.

Abstract: The present invention relates to a system including a robotic actuator configured to engage an electrical sensor, a platform configured to retain a multi-well plate, and at least one processor configured to control the robotic actuator to dip the electrical sensor into wells filled with an analyst liquid, a sample liquid, a pre-detection liquid and a detection liquid. The present invention further relates to a corresponding method for determining the presence and/or concentration of a target molecule in a sample, for determining the binding kinetics and binding affinity of molecules and for determining the conformality structure.

Abstract: Provided herein are systems, devices and methods for retaining chromatographic minicolumns in a holding fixture. A retaining apparatus may be provided with a pair of laterally spaced apart upright supports and a horizontal beam spanning between the upright supports to hold them in the laterally spaced apart relationship. The apparatus may be configured to contact a holder plate only along peripheral edges of the holder plate. The apparatus may be configured to slide along the holder plate after the minicolumns have been inserted therein, thereby retaining the minicolumns in the holder plate but allowing access to the minicolumns through vertical holes in the horizontal beam.

Abstract: An automatic analyzer can be miniaturized as a whole and can accurately determine presence or absence of a sample container and a type thereof. The automatic analyzer of includes a conveyance line for conveying a loaded sample container; an identification information reading device that reads identification information attached to the sample container; and a container height detection device. The identification information reading device and the container height detection device perform reading and detection when the sample container is at a common position on the conveyance line. The container height detection device includes sensors having different height ranges for a detection target, a part of the sensors being disposed on the same side as the identification information reading device with respect to the conveyance line, and the rest of the sensors being disposed on the opposite side of the identification information reading device with respect to the conveyance line.

Abstract: The present disclosure relates to a technology related to an automated analysis system using individually operated biological devices, an analysis method, and a storage medium, wherein the system and the method enable an analysis sample to be prepared and analyzed by operating together with an automated analysis system through the operative connection of stand-alone devices.

Abstract: Disclosed is a specimen analyzer that uses a specimen rack in which a plurality of specimen containers can be held to analyze a specimen in each of the specimen containers, and the specimen analyzer includes: a measurement unit configured to measure the specimen; and a transport unit comprising a first placement region which is disposed in front of the measurement unit and in which a plurality of the specimen racks can be placed, and a second placement region which is disposed in a left-right direction relative to the first placement region and in which a plurality of the specimen racks can be placed, the transport unit configured to transport the specimen racks placed in the first placement region and the second placement region to a start position at which the measurement unit starts measurement.

Abstract: A sample holder shuttle for holding a sample holders for laboratory processing, such as PCR thermal cycling, focused acoustic energy treatment and others. Sample holders can be received at openings of the shuttle such that a plurality of sample holders can be held by the shuttle and moved or otherwise manipulated simultaneously. A cover can engage with sample holders, e.g., to press the sample holders downwardly into contact with the shuttle.

Abstract: Delivering a receptacle to an instrument comprises supporting a receptacle in a carriage when the carriage is positioned at a first location of the instrument, activating a sensing system coupled to the carriage to confirm that the receptacle is supported by the carriage, moving the carriage, and the receptacle supported therein, to a second location of the instrument, applying a clamping force to the receptacle as the carriage moves from the first location to the second location, at the second location, extracting at least a portion of a fluid contained in the receptacle using a fluid extraction device of the instrument, moving the carriage, and the receptacle supported therein, from the second location to the first location, and releasing the clamping force from the receptacle as the carriage moves from the second location to the first location.

Abstract: A metering head for receiving and metering at least two media, having at least two media inlets, one or multiple dispensing terminals, and fluid lines connecting the media inlets to the one or multiple dispensing terminals. The one or multiple dispensing terminals each have at least two fluidically separated media outlets. A metering system, having a metering head, at least one connecting element for fluidically connecting the metering head to a carrier substrate or to a microfluidic cartridge, and optionally a connection piece, wherein the dispensing terminal is designed to receive the connecting element directly or to receive the connecting element indirectly via the connection piece.

Abstract: Devices and methods for rapidly and incrementally or continuously, measuring rheological properties of polymers under different shear rates. The device includes a pump configured to accept a continuous stream of sample solution during an interval of time, an injector configured to inject a flow of the sample solution through two or more viscometers, and a computing and processing device configured to monitor and measure rheological properties of the solution under at least two shear rates simultaneously in the two or more viscometers.

Abstract: An apparatus for measuring the wind velocity and wind direction that uses a three dimensional 360-degree rotating ambient flow intake assembly, a multiphase flowmeter and a blower, and establishes wind functions and further incorporates a neural network to reduce Gaussian noise. Such an apparatus can have high utility in systems that detect emissions.

Abstract: The present disclosure provides an acceleration sensor. The acceleration sensor includes: a substrate; a first mass portion supported by the substrate so as to be movable along a thickness direction of the substrate; and a beam portion supporting the first mass portion on the substrate. The beam portion includes: a second mass portion having a mass less than a mass of the first mass portion; a first spring portion connecting the first mass portion with the second mass portion; and a second spring portion having a spring constant smaller than a spring constant of the first spring portion and connecting the second mass portion with the substrate. A movable electrode of a sensor element is disposed on the second mass portion and configured to detect an acceleration along the thickness direction of the substrate. A fixed electrode of the sensor element is disposed on the substrate.

Abstract: A dual axis accelerometer with a single proof mass measures in-plane acceleration (e.g., either along an x-axis or a y-axis), out-of-plane acceleration (e.g., normal to an x-y plane), and tilt of a fixed portion of a MEMS layer (e.g., normal to the x-y plane). In response to a tilt measurement, the dual-axis accelerometer compensates any offset (e.g., variability) of the out-of-plane accelerometer in order to maintain offset stability. In some embodiments, multiple dual axis accelerometers, perpendicularly configured, may be implemented via processing circuitry to offer three axis sensitivity capability.

Abstract: A method for processing sensor signals of a sensor unit for a vehicle. The sensor unit has at least two acceleration sensors of the same type. The method includes reading in a first sensor signal from a first acceleration sensor of the sensor unit and at least one other sensor signal from at least one other acceleration sensor of the sensor unit. Each sensor signal represents acceleration data acquired by the corresponding acceleration sensor. A fusion of the sensor signals of the acceleration sensors is performed using a processing specification to generate a processed sensor signal. The processing specification takes into account predefined interference acceleration parameters to which the acceleration sensors are subject when they are installed in the vehicle.

Abstract: A contacting device is provided for an impedance limit switch, the contacting device including: a printed circuit board having an electrically conductive contact surface; a radially disposed annular spring configured to establish electrical contact between the electrically conductive contact surface of the printed circuit board and at least one electrode of the impedance limit switch; and a non-conductive housing element. An impedance limit switch is also provided, including the contacting device; an electronic cup; and a measuring head configured to carry out a conductive and/or capacitive measurement and to record measurement data, the contacting device being configured to transmit the measurement data from the measuring head to the electronic cup via the contacting unit.