Abstract: An image acquisition apparatus includes first and second imaging elements, an image formation optical system, a subject stage that supports a subject, and a control unit. The image formation optical system forms an image of the subject on light-receiving surfaces of the first and second imaging elements. The control unit acquires imaging results by imaging a correction mark disposed on the subject stage while changing a relative position between the first imaging element and the correction mark in an optical axis direction of the image formation optical system. The control unit moves, in accordance with the imaging results, the first imaging element or subject stage in the optical axis direction so the first imaging element and the correction mark are conjugate, acquires an imaging result by imaging the correction mark, and moves, in accordance with the acquired imaging result, the second imaging element in the optical axis direction.

Abstract: The illumination optical system is configured to illuminate a sample placed on an object plane with light. The illumination optical system includes multiple light source areas which are mutually coherent and arranged separately from one another in a pupil plane of the illumination optical system. Among distances from a center of a pupil of the illumination optical system to centers of the multiple light source areas, at least one of the distances is different from the other distances.

Abstract: An optical zoom device for setting an imaging scale of an imaging device, which is configured for imaging an object on an image plane of an image recording device using a microscope objective, comprising an optical element arrangement is disclosed. The optical element arrangement includes an object-side zoom entrance for optical connection to an objective exit, in particular a collimated objective exit, of the microscope objective and includes an image-side zoom exit for optical connection to an image recording entrance of the image recording device.

Abstract: Exemplary embodiments provide solid-state microscope (SSM) devices and methods for processing and using the SSM devices. The solid-state microscope devices can include a light emitter array having a plurality of light emitters with each light emitter individually addressable. During operation, each light emitter can be biased in one of three operating states including an emit state, a detect state, and an off state. The light emitter can include an LED (light emitting diode) including, but not limited to, a nanowire based LED or a planar LED to provide various desired image resolutions for the SSM devices. In an exemplary embodiment, for near-field microscopy, the resolution of the SSM microscope can be essentially defined by the pitch p, i.e., center-to-center spacing between two adjacent light emitters, of the light emitter array.

Abstract: A system and method for correcting the color of microscope images is described wherein a microscope, equipped with a variable light source and at least one microscope setting selector operates in conjunction with an image recording device having at least one image setting selector with a plurality of settings, and is configured to record at least one white balanced sample image of a sample. The system and method also includes the use of at least one white balanced calibration image of an integral transmissive color filter array of known transmission values in combination with an image processor executing code in order to color calibrate the sample images based on the calibration image.

Abstract: A user interface for operation of a scanning electron microscope device that combines lower magnification reference images and higher magnification images on the same screen to make it easier for a user who is not used to the high magnification of electron microscopes to readily determine where on the sample an image is being obtained and to understand the relationship between that image and the rest of the sample. Additionally, other screens, such as, for example, an archive screen and a settings screen allow the user to compare saved images and adjust the settings of the system, respectively.

Abstract: To provide a microscopic imaging device in which a measuring object can be easily imaged using measurement light having a desired pattern, in which the pattern of measurement light can be changed and a phase of the pattern can be moved, without arranging a mechanical mechanism. An arbitrary pattern of a plurality of patterns of measurement light is instructed. The measurement light having an instructed pattern is generated by a light modulation element, and is applied on a measuring object. A spatial phase of the generated pattern is sequentially moved on the measuring object by a predetermined amount by the light modulation element. A plurality of pieces of pattern image data generated at a plurality of phases of the pattern is synthesized based on the light receiving signal output from the light receiving section to generate sectioning image data indicating an image of the measuring object.

Abstract: To provide a microscopic imaging device capable of easily switching the imaging method. During sectioning observation and normal observation, a measuring object is irradiated with pattern measurement light and uniform measurement light generated by a light modulation element, respectively. The measuring object is irradiated with the pattern measurement light and the uniform measurement light through a common light path. During the sectioning observation, a spatial phase of the pattern is sequentially moved on the measuring object by a predetermined amount by the light modulation element, and sectioning image data indicating an image of the measuring object is generated based on a plurality of pieces of image data generated at a plurality of phases of the pattern based on the light receiving signal. During the normal observation, normal image data indicating an image of the measuring object is generated based on the light receiving signal.

Abstract: To provide a microscopic imaging device, a microscopic imaging method, and a microscopic imaging program capable of detecting a focused position through an appropriate method corresponding to the imaging method. In a sectioning observation, a measuring object is irradiated with pattern measurement light, and sectioning image data is generated. In a normal observation, the measuring object is irradiated with uniform measurement light to generate normal image data. Relative positions of an objective lens and the stage are changed a plurality of times in an optical axis direction of the objective lens by a focus position adjustment mechanism. When the sectioning observation is instructed, a focused position is detected based on the value of each piece of pixel data of the sectioning image data. When the normal observation is instructed, a focused position is detected based on a local contrast of the normal image data.

Abstract: In the image capturing apparatus, the optical path difference producing member is disposed on the second optical path. Thereby, at the second imaging device, it is possible to suppress the amount of light on image pickup of an optical image which is focused at the front of an optical image made incident into the first imaging device (front focus) and on image pickup of an optical image which is focused at the rear thereof (rear focus) and also to secure the amount of light on image pickup by the first imaging device. Further, in the image capturing apparatus, based on a scanning velocity v of the stage and an interval d between the first imaging region and the second imaging region, a waiting time is set from image pickup at the first imaging region to image pickup at the second imaging region. As a result, light from the same position of the sample is made incident into the first imaging region and the second imaging region. Thus, it is possible to control a focus position at high accuracy.

Abstract: The invention relates to a technique of improving a contrast of a lower-layer pattern in a multi layer by synthesizing detected signals from a plurality of detectors by using an appropriate allocation ratio in accordance with pattern arrangement. In a charged particle beam device capable of improving image quality by using detected images obtained from a plurality of detectors and in a method of improving the image quality, a method of generating one or more output images from detected images corresponding to respective outputs of the detectors that are arranged at different locations is controlled by using information of a pattern direction, an edge strength, or others calculated from a design data or the detected image.

Abstract: A microscope capable of controlling the position and fluorescent recording of an object under observation such as cells is provided with the fluorescent observation method using the microscope.

Abstract: A microscope apparatus and control method. An image processing unit obtains an image on a plane observation cross-section inside a sample. A drive control unit controls a galvano-scanner and a z drive unit, and inclines the entire observation cross-section in a direction where the cross-section of an observation target that appears in the image becomes long. When an edge portion of the cross-section of the observation target is detected in the observation cross-section while gradually inclining the observation cross-section, the drive control unit uses an axis passing through the edge portion as the center to incline a part of the observation cross-section located on a side, where the cross-section of the observation target does not appear, from among the sides having this axis as a boundary, in a direction where the cross-section of the observation target appears, so as to form the observation cross-section having a profile which is folded.

Abstract: Stabilization, via active-feedback positional drift-correction, of an optical microscope imaging system in up to 3-dimensions is achieved using the optical measurement path of an image sensor. Nanometer-scale stability of the imaging system is accomplished by correcting for positional drift using fiduciary references sparsely distributed within or in proximity to the experimental sample.

Abstract: A self-illuminating microscope slide with a biopsy specimen disposed thereon comprises LEDs on the ends of the slide to illuminate the specimen through total internal reflection. A camera device captures a single digital image of the entire specimen in high resolution and large field of view encompassing the specimen.

Abstract: An instrument and method for scanning all or part of a large specimen mounted on a specimen holder takes a plurality of measurements of each pixel in the whole or part of the specimen being scanned at a plurality of exposure values. A computer controls the movement of the specimen holder during scanning and again of the detector to produce a digitized image of all or part of the specimen with larger dynamic range than the dynamic range of the detection system. In a further embodiment, the instrument can scan two successive, identical strips at a different exposure values and combine the images from the two scans into one digitized image having a larger dynamic range than the dynamic range of the detection system.

Abstract: The present inventions are related to systems and methods for determining characteristics of a material. The characteristics may include, but are not limited to, crystallographic texture.

Abstract: The present invention provides a contour extraction technique capable of resolving not only spuriousness, duplication, or branches in the contours of a sample pattern, but also discontinuities in the contours. A thinning process is performed with respect to design data for generating a sample pattern, and pattern in/out definition information defining the inside and outside of a pattern formed on a target sample is generated. Then, based on the Marker Controlled Watershed Segmentation method, region segmentation is performed by expanding regions indicated by the pattern in/out definition information while referencing pixel values of an edge-enhanced image of a target sample image, and pattern contours are generated.

Abstract: A conveying device including: a storage unit storing two or more sheets of slide glasses to be subjected to a predetermined treatment; a stage holding only one sheet of slide glass to be subjected to the treatment; a supply arm by which one sheet of slide glass to be subjected to the treatment is picked up from the storage unit and supplied onto the stage; a discharge arm by which the slide glass mounted on the stage is picked up and discharged in the storage unit; a moving unit operable to move the supply arm and the discharge arm in an integral manner so as to bring the supply arm or the discharge arm into proximity to each of the storage unit and the stage; and a control unit operable to control the supply arm, the discharge arm and the moving unit.

Abstract: Provided is a microscope including: dark field illumination and bright field illumination which illuminate a preparat where a sample mounted on a slide glass is covered with a cover glass and a mounting agent; an image capturing unit which acquires a dark field image by image-capturing the preparat illuminated by the dark field illumination and which acquires a bright field image by image-capturing the preparat illuminated by the bright field illumination; and a magnified portion image acquisition area determination unit which detects an edge of the cover glass in the preparat based on the dark field image and the bright field image acquired by the image capturing unit and determines an internal area of the detected edge of the cover glass as a magnified portion image acquisition area of the sample.

Abstract: The present invention discloses an image processing method and apparatus, where the method includes: downscaling an image to be processed; identifying identifiable targets in the downscaled image, and localizing temporary unidentifiable targets in the downscaled image; identifying the temporary unidentifiable targets at positions, corresponding to localized positions in the downscaled image, in the image to be processed. According to the present invention, only one object lens is needed to obtain the image to be processed, and no switching process between a high magnification lens and a low magnification lens is required during a target screening process; so that image identification speed is increased, and mechanical errors and offset in positioning are avoided.

Abstract: A method is provided for controlling a Tracking AutoFocus (TAF) portion of a machine vision inspection system including an imaging portion, a movable workpiece stage, a control portion, and graphical user interface (GUI). The TAF portion automatically adjusts a focus position of the imaging portion to focus at a Z height corresponding to a current surface height of the workpiece. The method includes providing the TAF portion, and providing TAF enable and disable operations, wherein: the TAF disable operations comprise a first set of TAF automatic interrupt operations that are automatically triggered by user-initiated operations that include changing the Z height, and the TAF disable operations may further comprise automatic interrupt operations that are automatically triggered based on at least one respective TAF Z height surface tracking characteristic exceeding a previously set TAF disable limit for that respective TAF Z height surface tracking characteristic.

Abstract: A color degradation compensation method is provided. The color degradation compensation method includes the steps as follows. Two images are captured by an image capturing device, wherein the two images have an overlapped area. A calculating procedure is constructed. At least one position in the overlapped area is selected and color values of a plurality of points in the position on each of the two images are read, wherein the points of each image are corresponded to the same position in the overlapped area. A ratio of the two color values is calculated. A degradation standard deviation of the two color values is calculated by the calculating procedure. The color values of the two images are calibrated to compensated color values through the calculating procedure in accordance with the degradation standard deviation.

Abstract: An imaging apparatus is disclosed which uses a super-oscillatory lens to obtain sub-diffraction limit resolution. The super-oscillatory lens is arranged to receive a light beam from a light source, the lens having a pre-defined pattern to spatially modulate the light beam in amplitude and/or phase so that it focuses the light beam to a focus at a first focal point having a full width half maximum of less than half the wavelength. Collection optical elements are arranged to focus the first focal point to a second focal point conjugate to the first focal point. An object for imaging is scanned over the first focal point and a detector is arranged to collect light from a collection region centered on the second focal point.

Abstract: Provided are a magnification observation device, a magnification observation method, and a magnification observation program in which connected image data can be efficiently obtained in a short period of time when re-imaging an object to obtain the connected image data corresponding to a plurality of unit regions. A plurality of unit regions on a surface of an observation object are imaged, and a plurality of pieces of image data respectively corresponding to the plurality of unit regions are generated. An image of the object including the plurality of unit regions is displayed as a region presentation image. When any of the plurality of unit regions is selected by a selection instruction from a user, the selected unit region is re-imaged, to generate image data corresponding to the selected unit region as re-imaged data.

Abstract: Noise reduction processing for measured spectrum data is performed without any information loss due to discrete data characteristics of the measured spectrum data. Optical spectra in one or more cross-sections are measured through use of a signal correlated with a substance distributed in a biological tissue, and a biological tissue image having reduced noise is reconstructed from the spectra.

Abstract: Time lapse observation method includes: before a process to obtain a first time lapse image, capturing an image of a reference area on a sample being a partial area of a target area or an area in a vicinity of the target area being a smaller area than the target area to obtain a reference image; storing a position of a capturing area in capturing the reference image as a reference position; before a process to obtain the time lapse image performed, setting a position of the capturing area sequentially at different positions in the optical axis direction of an objective including the reference position and capturing an image at each of the positions to obtain comparison target images; and matching the capturing area with the target area, based on a comparison result of the reference image and the comparison target images.

Abstract: A method and apparatus for focusing a device for imaging a biologic sample is provided. A method aspect includes the steps of: disposing lenslets within a biologic sample, which lenslets have a height and a refractive index, which refractive index is different from that of the sample, wherein one or both of the imaging device and the sample are relatively locatable so a focal position of the imaging device can be moved along the height of the lenslets; imaging a portion of the sample including lenslets using transmittance at one or more wavelengths; determining an average light transmittance intensity of the sample at the wavelengths; determining an average light transmittance intensity of a region of each lenslet at the wavelengths; and determining the focal position of the imaging device using the average light transmittance intensity of the sample and the average light transmittance intensity of the region of the lenslets.

Abstract: A system comprises a first beamsplitter module and a second beamsplitter module aligned with the first beamsplitter module along an alignment line. In certain embodiments, each beamsplitter module can split a beam traveling along a first optical path into a first split beam and a second split beam within a spectral range. Each beamsplitter module can transmit the first split beam along the first optical path and direct the second split beam along a second optical path substantially orthogonal to the first optical path and to the alignment line. In certain embodiments, each beamsplitter module can receive a first beam traveling along a first optical path and a second beam traveling along a second optical path that is substantially orthogonal to the first optical path and to the alignment line. Each beamsplitter module can combine the second beam with the first beam to yield a combined beam and transmit the combined beam along the first optical path.

Abstract: An apparatus and method of quantitatively obtaining a measurement of pollen of a plant. One method of counting comprises imaging the sample with the pollen well-distributed in the focal plane of the imager. Image evaluation software can identify and count objects in the image that are consistent with pollen. Total pollen count for the plant can be derived from the count of pollen of the sample, proportionality of the sample volume to the starting volume, and proportionality of area of sample imaged to total area of sample. Pollen quantification can be used for research or commercial production decisions relative to the plant or its seed.

Abstract: A system for characterizing cells takes a series of digital images of a sample containing the cells. Each of the images is taken at a different plane of focus. One of the images is determined to have been taken at a plane of best focus. The system analyzes the digital image taken at the plane of best focus and at least one other of the digital images to classify cells in the sample as either live or dead.

Abstract: An imaging apparatus includes an imaging unit, an illuminating unit, and an image-characteristic setting unit. The imaging unit images an object to acquire an image of the object. The illuminating unit includes a light source configured to apply illumination light beams different in optical characteristics to the object. The image-characteristic setting unit sets the image characteristics of the image data, then refers to light source characteristic information, and sets to the illuminating unit, the intensity, distribution pattern, spectrum distribution or polarization characteristic of at least one illumination light beam. The imaging unit acquires effectively image data having the image characteristics set.

Abstract: A microscope apparatus organizes and stores a plurality of types of image information acquired by a plurality of image acquisition methods at different timings. The microscope apparatus includes a time counting unit for counting time, a plurality of different image acquisition units, and a storage unit for storing image information, when acquired by any one of the image acquisition units, and timing information counted by the time counting unit, by having them associated with each other. Even if the image acquisition units acquire different types of image information at different timings, the timing information can be used to call up the image information stored in the storage unit in a chronological order.

Abstract: Disclosed is a diffraction microscopy capable of reducing influence of an increase in the incident angle range of a beam. Specifically disclosed is a diffraction microscopy in which a beam is incident on a sample, in which the intensity of a diffraction pattern from the sample is measured, and in which an image of an object is rebuilt using Fourier interactive phase retrieval on the basis of the measured intensity of the diffraction pattern. In this method, Fourier interactive phase retrieval is performed using deconvolution on the diffraction pattern subjected to convolution by the increase in the incident angle range of the beam.

Abstract: A method and apparatus for simulating depth of field (DOF) in microscopic imaging, the method comprising computing a blur quantity for each pixel of an all-focus image, performing point spread function operations on one or more regions of the all-focus image, computing intermediate and normalized integral images on the regions and determining an output pixel for the each pixel based on the intermediate and normalized integral images.

Abstract: A system (100) and method for creating three dimensional images using probe molecules is disclosed and described. A sample is mounted on a stage (160). The sample has a plurality of probe molecules. The sample is illuminated with light, causing the probe molecules to luminesce. The probe luminescence can be split into at least four paths corresponding to at least four detection planes corresponding to object planes in the sample. The at least four detection planes are detected via a camera (155). Object planes in corresponding recorded regions of interest are recorded in the camera (155). A signal from the regions of interest is combined into a three dimensional image.

Abstract: Provided is a magnification observation device capable of easily moving an observation object in a desired direction even when a stage is rotated, and capable of preventing a desired region of the observation object from going outside an imaging region due to rotation of the stage. The observation object is placed on the placement surface of the stage. The observation object is imaged by an imaging unit, to acquire image data of the imaging region. Based on the image data acquired by the imaging unit, an image of the observation object is displayed by the display part as an observed image. The stage is moved relatively with respect to the imaging unit along an xt-axis and a yt-axis. In this case, moving amounts along the xt-axis and the yt-axis are controlled based on a rotational angle detected by a rotational angle detecting part.

Abstract: To provide a microscope system enabling the user to accurately observe the surface of the observing target in a short time. A sensitivity parameter for adjusting a value of the pixel data corresponding to a plurality of pixels is set. The pixel data is acquired with a first sensitivity value set as the sensitivity parameter, and the number of pixels in which the peak value of the pixel data is smaller than or equal to a threshold value is detected. A ratio of the detected number of pixels with respect to the total number of pixels region is calculated, where the pixel data is acquired by a second sensitivity value different from the first sensitivity value when the ratio is greater than or equal to a reference value, and the pixel data is not acquired by the second sensitivity value when the ratio is smaller than the reference value.

Abstract: The invention pertains to the field of image processing in digital pathology. It notably proposes a method for processing a first digital image, representing a sample in a region, and which image has been acquired from the sample by means of a microscopic imaging system (1) and is stored in a multi-resolution image data structure (80), comprising the steps of:—retrieving (104) a sub-region of the first digital image at a first resolution, —executing (105) a transform function on the retrieved sub-region, the transform function modifying a content of the sub-region according to at least one metric derived from a second resolution representation of the first digital image.

Abstract: To provide an observation apparatus, an observation program, and an observation method that are suitable for the detection and observation of an observation target object. An observation apparatus according to the present technology includes a microscope optical system, an imaging unit, a spectroscopic unit, and a detection unit. The imaging unit captures an image via the microscope optical system. The spectroscopic unit acquires an absorption spectrum or a Raman spectrum in an ultraviolet, visible, or infrared area via the microscope optical system. The detection unit detects an observation target object in an observed sample by using the absorption spectrum or the Raman spectrum.

Abstract: A method of creating a phase contrast image is provided. In some embodiments the method comprises illuminating the target region of a sample with a first light source to provide a first oblique back illumination of the target region of the sample, and detecting a first phase contrast image from light originating from the first light source and back illuminating the target region of the sample. In some embodiments the method further comprises illuminating the sample with a second light source to provide a second oblique back illumination of the target region of the sample, and detecting a second phase contrast image from light originating from the second light source and back illuminating the target region of the sample. In some embodiments a difference image of the target region of the sample is created by subtracting the second phase contrast image of the target region of the sample from the first phase contrast image of the target region of the sample.

Abstract: In one embodiment, a method includes receiving an image of a user's eye at a device having a screen and a camera operable to input the image, processing the image to identify one or more characteristics of the user's eye for use in determining if the user is having difficulty viewing data displayed on the screen, and magnifying the data displayed on the screen if the user is having difficulty viewing the data. An apparatus and logic are also disclosed.

Abstract: A method for improving depth for field (DOF) in microscopic imaging, the method comprising combining a sequence of images captured from different focal distances to form an all-focus image, comprising computing a focus measure at every pixel, finding the largest peaks at each position in the focus measure as multiple candidate values and blending the multiple candidates values according to the focus measure to determine the all-focus image.

Abstract: Systems and methods for structured illumination super-resolution phase microscopy are disclosed. According to an aspect, an imaging system includes a light source configured to generate light. The system also includes a diffraction grating positioned to receive and diffract the output light. The system also includes a sample holder positioned to receive the diffracted light for transmission through a sample. Further, the system includes an image detector positioned to receive the light transmitted through the sample and configured to generate image data based on the received light. The system also includes a computing device configured to apply subdiffraction resolution reconstruction to the image data for generating an image of the sample.

Abstract: A system and method of high-speed microscopy using a two-photon microscope with spectral resolution. The microscope is operable to provide spectrally resolved, multi-dimensional images from a single scan of a sample. The microscope may include one of a multi-beam point scanning microscope, a single beam line scanning microscope, and a multi-beam line scanning microscope. The microscope includes a descanning arrangement such that emitted fluorescence is static on a receiving detector. The detector is a narrow detector with a width at least half the size of the length, to reduce the amount of pixel data being transmitted and improve scan speeds. The microscope may also incorporate one or more binning techniques whereby pixels are binned together to improve resolution or scan speeds.

Abstract: An imaging assembly for the viewing, imaging, and analysis of biological, chemical, and/or biochemical samples in gels or other substrates, in which an adjustable camera and lens module, a reflex mirror, and a focal plane mirror, are configured to bend or fold an optical path in order to image a target region, and where the optical path can be reflected along non-orthogonal angles. The imaging assembly is configured to reduce the overall size of the imaging apparatus due to the angles at which the mirrors and camera and lens assembly are positioned relative to each other, which allows for the imaging of relatively larger samples in the target region.

Abstract: A microscope system (100) includes a microscope (10) and an observation unit (20) provided separately from the microscope. The microscope includes a microscope objective lens (3), an image pickup element (31) disposed at a position at which an image is formed through the microscope objective lens, and a first control apparatus (32) connected with the image pickup element. The observation unit includes a second control apparatus (21), a display device (22, 22a, 22b) connected with the second control apparatus, and a magnifier optical system (25a, 25b) arranged at a predetermined distance from the display device. The microscope system further includes a communication apparatus (21) for communication between the first control apparatus and the second control apparatus so that an image picked up by the image pickup apparatus is displayed on the display device.

Abstract: An optical observation apparatus has an optical image channel for viewing optical field-of-view images, a processing unit to generate a first electronic insertion image via a first electronic image channel, and a data insertion image projector for generating an optical insertion image from the first electronic insertion image and inserting that image into the optical field-of-view image. An acquisition device acquires the optical field-of-view image without the optical insertion image inserted by the data insertion image projector. A second electronic image channel has a superimposition module, that superimposes the electronic field-of-view image with an electronic insertion image and provides the superimposed images via the second electronic image channel. The processing unit generates a second electronic insertion image, provides available display data elements, and makes it possible to assemble the electronic insertion images and assign them to different electronic image channels.

Abstract: The present invention concerns a system and method for calibration and adjustment of the pixel color values represented within a digital image of a sample by a transmission microscope. Furthermore the present invention is directed to providing sufficient color information in order to generate a color mapping matrix that allows for the creation of a synthetic image to depict the sample under a desired illumination. The system and method provides a solution that generates a destination-device independent image that is configurable to any calibrated display device.

Abstract: The invention relates to a high resolution microscope for three-dimensionally determining the position of objects, in particular individual fluorophores, and preferably for the high spatial resolution luminescence microscopy of a sample, which is marked with marker molecules that can be activated or switched using a signal such that they can be induced to emit certain luminescent radiation only in the activated state. The object is represented by means of an imaging system, preferably the microscope lens, on a surface detector consisting of individual detector elements.