Abstract: A microscope for microscopy having a detection beam path and an illumination beam path. The microscope includes a filter wheel device, which is arranged in the detection beam path and/or illumination beam path and has a filter wheel, wherein the filter wheel is mounted to rotate about an axis, and wherein the filter wheel is divided into segments. Filters forming a first part-circle are arranged in a first part of the segments, such that said filters are consecutively introduced into the detection beam path or illumination beam path when the filter wheel is rotated. A camera records images at a predefined frequency. The filter wheel device includes a motor-driven shaft that rotates the filter wheel at a predefined rotation frequency. The microscope also comprises a control system for synchronising the image-recording frequency and rotation frequency based on the filters arranged on the filter wheel.

Abstract: A device for forming a high-resolution image of an object is provided. The device comprises: an electronic camera for capturing an intermediate image of the object, an illumination system for forming a spatial modulation pattern on the object; and a spatial demodulator for performing a spatial demodulation, which is at least partially matched to the spatial modulation pattern. A method for deriving a high-spatial-resolution image from a set of images captured from a structure of an object is derived, wherein the illumination of the object is spatially-modulated, wherein the illumination of the object has a spatial modulation pattern, which is substantially periodic, wherein one of at least one prevailing orientation of the periodic illumination is arranged substantially perpendicularly to at least one prevailing orientation of the structure of the object.

Abstract: An observation device is provided with a light source section, a detection section and an arithmetic operation section. The light source section emits light to a moving object from multiple directions. a detection section is disposed on a predetermined plane such that scattered light having an identical scattering angle enters at an identical position, outputs data temporally changing at a frequency corresponding to an amount of Doppler shift of light that reaches at each position on the predetermined plane. An arithmetic operation section performs a one-dimensional Fourier transform with respect to time variables, for data having a position in the first direction on the predetermined plane, a position in the second direction on the predetermined plane, and a time as variables, and extracts data having an identical incident angle relative to the object from the Fourier-transformed data, on the basis of Doppler Effect.

Abstract: A microscope system for stitching element images to generate a stitched image includes an element image obtaining unit configured to obtain the element image; a user image obtaining unit configured to capture a plurality of user-specified areas being an area of a sample specified by a user to obtain a plurality of user images; a rectangle area deciding unit configured to decide a rectangle area including the plurality of user-specified areas; a candidate area deciding unit configured to decide, as a candidate area, each of a plurality of areas having a size of a field of view of the element image obtaining unit arranged in a grid-like manner in the rectangle area so as to fill the rectangle area; and an element area selecting unit configured to select an element area to obtain the element image, from the plurality of candidate areas.

Abstract: Embodiments relate to systems and methods for sample image capture using integrated control. A digital microscope or other imaging device can be associated with a sample chamber containing cell, tissue, or other sample material. The chamber can be configured to operate using a variety of environmental variables, including gas concentration, temperature, humidity, and others. The imaging device can be configured to operate using a variety of imaging variables, including magnification, focal length, illumination, and others. A central system control module can be used to configure the settings of those hardware elements, as well as others, to set up and carry out an image capture event. The system control module can be operated to control the physical, optical, chemical, and/or other parameters of the overall imaging environment from one central control point. The variables used to produce the image capture can be configured to dynamically variable during the media capture event.

Abstract: A microscope comprises an imaging system comprising an objective having an objective lens and producing a magnified image of a focal plane of the imaging system, an image sensor disposed in the focal plane and outputting an electrical signal to the controller, and a display communicating with the controller. The controller converts signals of the image sensor into digital single images of the object, outputs the same to the display, detects a displacement of the microscope transverse to an optical axis of the objective lens relative to an object imaged, and compares the displacement with a threshold. If the displacement exceeds the threshold the controller controls a zoom system such that a total magnification is inverse monotone to a relative velocity between the microscope and the object, and/or stores the digital single images, composes them into a total image, and outputs the same to the display.

Abstract: An imaging method and corresponding apparatus according to an embodiment of the present invention enables measurement and visualization of fluid flow. An embodiment method includes obtaining video captured by a video camera with an imaging plane. Representations of motions in the video are correlated. A textured background of the scene can be modeled as stationary, with a refractive field translating between background and video camera. This approach offers multiple advantages over conventional fluid flow visualization, including an ability to use ordinary video equipment outside a laboratory without particle injection. Even natural backgrounds can be used, and fluid motion can be distinguished from refraction changes. Depth and three-dimensional information can be recovered using stereo video, and uncertainty methods can enhance measurement robustness where backgrounds are less textured. Example applications can include avionics and hydrocarbon leak detection.

Abstract: A surgical microscope 1 comprises an imaging system 2 providing a magnified multidimensional image of an object 3 disposable in a focal plane 4 of the imaging system 2 along at least one optical imaging path 2a, 2b. The imaging system 2 comprises an objective 5 having at least two lens groups 6, 7, through which the at least one optical imaging path 2a, 2b passes consecutively, and which define the focal plane 4 of the imaging system 2. At least one lens group 6 of the objective is moveable along its optical axis relative to the at least one other lens group 7 of the objective. The objective's first lens group 6 located directly adjacent to the focal plane 4 along the at least one optical imaging path 2a, 2b consists of at least three optical lenses 61, 62, 63 and has altogether a negative optical power.

Abstract: An image display device and a method for driving the same are discussed. The image display device includes a display panel, which includes a subpixel disposed at a crossing between a pair of gate lines and a data line to selectively display a 2D image and a 3D image, a panel driver including a gate driver for driving the pair of gate lines and a data driver for driving the data line, a controller for differently controlling the panel driver in response to a mode selection signal, and a patterned retarder for dividing light from the display panel into first polarized light and second polarized light.

Abstract: Provided is a microscope apparatus including a macro-image, acquisition section that acquires a macro image of a glass slide having a specimen mounted thereon; an extraction section that extracts the specimen in the acquired macro image; a block setting section that sets, when the extracted specimen is scattered to form a plurality of lumps, a plurality of blocks that include the lumps of the specimen; an area dividing section that divides an area that includes the specimen in each of the blocks into a plurality of small regions; and a micro-image acquisition section that acquires, for each of the small regions, a micro image while performing an automatic focusing operation for the specimen in the small region, in which the micro-image acquisition section searches for an autofocusing in-focus position in each of the blocks set by the block setting section.

Abstract: The present invention generally relates to super-resolution microscopy. For example, certain aspects of the invention are generally directed to a microscopy system comprising at least two objectives. In some embodiments, the microscopy system may also contain a non-circularly-symmetric lens. One or more images can be obtained using the objectives, for example, using stochastic imaging techniques such as STORM (stochastic optical reconstruction microscopy), optionally in conjunction with entities that are photoactivatable and/or photo switchable. The images obtained using the objectives may be compared, e.g., to remove noise, and/or to compare an entity present in both images, for instance, to determine the z-position of the entity. In some cases, surprisingly high resolutions may be obtained using such techniques, for example, resolutions of better than about 10 nm.

Abstract: Systems and methods for automated laser microdissection are disclosed including automatic slide detection, position detection of cutting and capture lasers, focus optimization for cutting and capture lasers, energy and duration optimization for cutting and capture lasers, inspection and second phase capture and/or ablation in a quality control station and tracking information for linking substrate carrier or output microdissected regions with input sample or slide.

Abstract: An image processing apparatus includes an image data acquisition unit, a display control unit, an area-information acquisition unit, and a detection unit. The image data acquisition unit acquires Z-stack image data including multiple layer images obtained by using a microscope apparatus. The display control unit displays at least one of the layer images on a display apparatus as an observation image. The area-information acquisition unit acquires information about a target area in the observation image specified by a user. The detection unit detects in-focus information for a corresponding area for the target area in each of the layer images. The display control unit displays an image indicating a positional relationship between the target area and the corresponding area which is closer to an in-focus state than the target area, along with the target area on the display apparatus, based on the result from the detection unit.

Abstract: An electronic imaging flow-microscope for remote environmental sensing, bioreactor process monitoring, and optical microscopic tomography applications is described. A fluid conduit has a port on each end of a thin flat transparent fluid transport region. A planar illumination surface contacts one flat side of the transparent fluid transport region and a planar image sensing surface contacts the other flat side. Light from the illumination surface travels through the transparent fluid transport region to the planar image sensing surface, producing a light field affected by the fluid and objects present. The planar image sensing surface creates electrical image signals responsive to the light field. The planar illumination surface can be light emitting elements such as LEDs, OLEDs, or OLET, whose illumination can be sequenced in an image formation process. The flow microscope can further comprise flow-restricting valves, pumps, energy harvesting arrangements, and power management.

Abstract: The present invention relates in general to microscopy systems. In particular, the present invention relates to microscopes rendering digital images of samples, with the capability to digitally control the focus of the microscope system, and the software used to control the operation of the digital microscope system. Further, the present invention relates to a microscope structure that allows for compact and multi-functional use of a microscope, providing for light shielding and control with samples that require specific light wavelength characteristics, such as fluorescence, for detection and imaging. The microscope is adjustable, with a structure that can move along range(s) of motion and degree(s) of freedom to allow for ease of access to samples, shielding of samples, and manipulation of a display apparatus.

Abstract: The present invention relates in general to microscopy systems. In particular, the present invention relates to microscopes rendering digital images of samples, with the capability to digitally control the focus of the microscope system, and the software used to control the operation of the digital microscope system. Further, the present invention relates to a microscope structure that allows for compact and multi-functional use of a microscope, providing for light shielding and control with samples that require specific light wavelength characteristics, such as fluorescence, for detection and imaging.

Abstract: A wireless fiber optic endface inspector includes a video microscope capable of wirelessly transmitting video streaming signal of endface image in real-time to a display device. The video microscope includes a microscope optical system, an adapting tip for interfacing an endface, an LED light source, a camera module for receiving and converting the endface image into video streaming signal, a Wi-Fi AP board, and a battery for supplying power. The video microscope may be constructed by adding a Wi-Fi AP board and a battery to a conventional inspector microscope. The display device has software for detecting when the endface is focused. Once the endface is focused, the display device emits an audio signal to alert the operator, who may then trigger the display device to analyze the endface image using an endface analysis software or save it to a folder, or transmit the image to a remote server.

Abstract: The invention relates to a microscopy method for identifying target objects (32) having a predetermined optical property in material (6) to be analyzed.

Abstract: In an imaging device having an objective and a stage for holding a sample to be imaged, a method for autofocusing is presented. The method includes determining a measured focus value corresponding to at least a first of a plurality of logical image segments. Further, the method includes imaging the first logical image segment using the measured focus value. The method also includes determining a predicted focus value for a second of the plurality of logical image segments using the measured focus value and a stored focus variation parameter. In addition, the method includes imaging the second logical image segment using the predicted focus value.

Abstract: The invention relates to a system for automatically adjusting an exposure time to improve or otherwise optimize a dynamic range of a digital image. The system includes a camera configured to capture an image of a subject within the field of view at a first exposure time. The captured image is composed of multiple pixels, with each pixel having a respective intensity value. The system further includes a shutter or suitable control configured to control an exposure time of the camera. A controller configured to carryout the following steps including: (a) querying a frequency distribution of pixel intensity values; (b) determining an effective “center of mass” of such a distribution, or histogram, to determine an adjusted exposure time; and (c) capturing a second image of the subject at the adjusted exposure time thereby obtaining an image with an improved or optimal dynamic range.

Abstract: A loupe accessory comprising: a loupe, comprising a first lens characterized in a positive focal length of less than 350 mm, the first lens for producing substantially infinity rays from a relatively close object, and an infinity-focus camera, comprising an image sensor and a second lens disposed at a fixed distance from the image sensor, the fixed distance being a focal length of the second lens, wherein the infinity-focus camera is fixed to the first lens, for imaging the infinity rays on the image sensor, thereby a first portion of the infinity rays produced by the first lens is imaged by the infinity-focus camera, and a second portion of the infinity rays produced by a second portion of the first lens is directly viewed by a human eye.

Abstract: A microscope for monitoring optical coherence tomography includes a microscope image providing unit which is configured to include a first objective lens, a beam splitter, and an ocular lens which are sequentially disposed from the front of a sample mount and an optical coherent tomography (OCT) image providing unit which generates an OCT image and provides the OCT image to the beam splitter of the microscope image providing unit, wherein the OCT image providing unit generates the OCT image of the same position as the surface image of the sample and provides the OCT image to the beam splitter of the microscope image providing unit, and in the case where the OCT image is provided through the beam splitter to the OCT image providing unit, the microscope image providing unit simultaneously outputs the surface image and the OCT image of the sample to the ocular lens.

Abstract: The invention relates to a control device and to a method for controlling a motorized digital microscope. The control device comprises at least one first module (01) for controlling the hardware, the software and the work flow of the digital microscope during the examination of a specimen. According to the invention, the first module (01) comprises a first input means (02, 16) having force feedback for controlling functions of the digital microscope. In a method according to the invention, data input takes place via a first input means, wherein force feedback is generated via the success or progression of the input.

Abstract: Microscope slide scanner. In an embodiment the microscope slide scanner comprises a single enclosure unit that includes at least one objective lens, at least one line scan camera, at least one communication port, and at least one processor. The line scan camera may be configured to capture image data of a sample as a plurality of image stripes via the objective lens. The communication port provides communication over a network. The processor may align the plurality of image stripes into a contiguous image of at least a portion of the sample, and executes a web server that provides an operator interface over the network to one or more remote devices.

Abstract: Systems and methods for capturing image data using a line scan camera. In an embodiment, a line scan camera captures image data of a sample as a plurality of image stripes. A processor may coarsely align two or more of the plurality of image stripes according to a synchronization process while the line scan camera is capturing at least one of the plurality of image stripes. Subsequently, the processor may also finely align the two or more image stripes using pattern matching.

Abstract: An apparatus and method for ultrafast real-time optical imaging that can be used for imaging dynamic events such as microfluidics or laser surgery is provided. The apparatus and methods encode spatial information from a sample into a back reflection of a two-dimensional spectral brush that is generated with a two-dimensional disperser and a light source that is mapped in to the time domain with a temporal disperser. The temporal waveform is preferably captured by an optical detector, converted to an electrical signal that is digitized and processed to provide two dimensional and three dimensional images. The produced signals can be optically or electronically amplified. Detection may be improved with correlation matching against a database in the time domain or the spatial domain. Embodiments for endoscopy, microscopy and simultaneous imaging and laser ablation with a single fiber are illustrated.

Abstract: The invention relates to a digital microscope and to a method for optimizing a work process in such a digital microscope. The digital microscope comprises according to the invention at least one first monitoring sensor for observing a sample (08), a sample table (4), an optics unit (02) or a user, and a monitoring unit. In the method according to the invention, first operating data of the first operating sensor are acquired and analyzed and evaluated in an automated manner in the monitoring unit, in order to generate control data and to use said data for controlling the work process of the digital microscope.

Abstract: An cell contour formation apparatus includes a cell image acquiring unit, a subband image creating unit, a features calculating unit, a correcting unit, a contour forming unit. The subband image creating unit creates subband images including a low frequency image and a high frequency image. The features calculating unit calculates a local texture features from the high frequency image. The correcting unit corrects the high frequency image on the basis of the pixel value of the low frequency image and the texture features. The contour forming unit forms contours of cells included in the cell group on the basis of the corrected high frequency image.

Abstract: A stage assembly, an imaging system that uses the stage assembly, and methods for using the stage assembly in a high content screening system. The stage assembly includes a stage having a top surface and an opposing bottom surface and an opening extending between the top and bottom surfaces to receive a specimen plate. The stage assembly also includes a calibration sample bay formed in the stage. A calibration sample can also be secured within the calibration sample bay.

Abstract: An alignment system includes a stage configured to retain an object, an image-capturing device configured to capture the image of the field of view of the microscope, and a processing module configured to generate a virtual mask and superimpose the virtual mask with the image of the object. In one embodiment of the present invention, a method for operating a virtual mask system includes the steps of generating a virtual mask, placing a first object on a stage, capturing at least one image of the first object, and superimposing the virtual mask with the image of the first object by adjusting a position or an inclined angle of the stage or adjusting a capturing position of an image-capturing device by considering at least the virtual mask and the image of the first object.

Abstract: Systems, methods, and media for assessing the quality of a microscope slide image. In an embodiment, a plurality of focus point values are acquired for a sample on a microscope slide. Each focus point value comprises x-y coordinates indicating a location on the sample and a z coordinate indicating a focus height for the location on the sample. A best-fit surface is calculated based on the focus point values, and it is determined whether or not outlying focus point values exist based on the best-fit surface and the z coordinate for one or more of the focus point values. A scanned image of the microscope slide may be displayed which comprises, for each outlying focus point value, an overlay that identifies the location on the sample of the outlying focus point value based on the x-y coordinates for the outlying focus point value.

Abstract: An analysis apparatus includes a two-dimensional coordinate detecting unit and a three-dimensional coordinate determining unit. The two-dimensional coordinate detecting unit is configured to detect, with respect to a captured image group obtained by capturing an analysis specimen including an analysis target object at a plurality of focal depths, a two-dimensional coordinate candidate being a candidate of a plane coordinate of the analysis target object in each captured image. The three-dimensional coordinate determining unit is configured to determine, based on a position relationship of the plane coordinate candidates between the captured images, a three-dimensional coordinate candidate being a candidate of a three-dimensional coordinate of the analysis target object.

Abstract: There is provided an image processing apparatus which can prevent the case where it is difficult to perform highly accurate diagnosis using combined boundary regions in a combined image. An image processing apparatus includes image data acquisition means, combined-image data generation means, and combined-boundary-region display data generation means. The image data acquisition means acquires multiple pieces of divided image data obtained by capturing images of multiple regions into which a captured area for an imaging target is divided. The combined-image data generation means generates combined-image data on the basis of the multiple divided image data. The combined-boundary-region display data generation means generates display image data to be used for an observer to recognize combined boundary regions in the combined-image data. The combined-boundary-region display data generation means changes at least one of a color and a brightness for all of the combined boundary regions included in a display area.

Abstract: An image processing apparatus includes: an image inputting unit configured to input a plurality of images having a portion where at least a part of subjects of the plurality of images is common to one another; and a correction image generating unit configured to generate a correction image used for correcting the plurality of images. The correction image generating unit includes: a difference acquiring unit configured to acquire a difference between luminance of pixels from two arbitrary images of the plurality of images, the two arbitrary images having a common portion where the subjects are common to one another; and a shading detection unit configured to detect a shading component in the arbitrary images based on the difference.

Abstract: Provided is a technique that makes it possible to inexpensively image a miniscule target located inside or on the surface of a light-transmitting substrate. An imaging device (100) is provided with the following on opposite sides of the substrate (60): a hole (34) that functions as a point light source; and an imaging element (53) that performs the imaging. Light from the hole (34) reaches the imaging plane (53A) of the imaging element (53) at a magnification of L2/L1, allowing magnified imaging without a lens.

Abstract: Disclosed is a digital microscope system capable of controlling two or more microscope units with a controlling unit. The digital microscope system includes (i) two or more microscope units, (ii) a camera interface, (iii) a controlling unit and (iv) a light output device. The microscope unit includes a lens tube, a digital camera, a light terminator and a stand. The camera interface includes two or more camera interface (I/F) channels and a camera interface channel selector. The controlling unit includes a memory, a display device, a CPU (Central Processing Unit) and a power supply.

Abstract: A microscope has a light source for generating a light beam having a wavelength, ?, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction.

Abstract: A phase distribution measurement method inside a biological sample includes taking in an optical image of the sample formed by a microscope to form a plurality of images with different image contrasts; calculating a component corresponding to phase distribution of the sample and a component corresponding to other than the phase distribution, and dividing the component corresponding to the phase distribution by the component corresponding to other than the phase distribution to forma normalized phase component image; breaking down the phase component image into a plurality of frequency components; performing a deconvolution process to each of the frequency components using an optical response character corresponding to each, and calculating phase distribution of a refraction component and phase distribution of a structure component; and calculating phase distribution of the sample by compounding the phase distribution of the refraction component and the phase distribution of the structure component.

Abstract: An apparatus and method for imaging a biologic fluid sample quiescently residing within a chamber is provided. The chamber includes a first panel and a second panel, between which the biologic fluid sample quiescently resides. At least one of the first and second panels is flexible. The chamber has one or more fields that are each defined by a cross-sectional area. The apparatus comprises a field illuminator, a chamber flattener, a positioner, and an image dissector. The field illuminator has an objective lens. The chamber flattener has a platen with a window and a cover plate. The chamber flattener is operable to cause the chamber to assume a substantially uniform Z-axis position for substantially all of the fields within the chamber. The positioner is adapted to position the objective lens and the chamber relative to one another. The image dissector is adapted to image the sample residing within the chamber.

Abstract: A digital microscope apparatus includes a first imaging unit including a first imaging device and a first optical system including an objective lens configured to enlarge first and second images of a preparation that holds a sample, the first image being formed on the first device through the first system, a second imaging unit including a second optical system that is branched from the first system and has a depth of field larger than the first system and a second imaging device on which the second image is formed through the second system, and a controller configured to calculate a provisional in-focus position of the lens based on the second image, determine an area of the first device, from which the first image is read, and search for, based on the read image, an in-focus position of the lens in a predetermined range based on the provisional in-focus position.

Abstract: Disclosed herein are embodiments of imaging biological specimens. An imaging system can include a microscope for directly viewing the biological specimen and a multi-spectral imaging apparatus for outputting digitally enhanced images, near-video rate imaging, and/or videos of the specimen. An imaging system can include a digital scanner that digitally processes images to produce a composite image with enhanced color contrast of features of interest.

Abstract: A mobile microscopy apparatus usable in connection with a mobile computing device comprising a memory unit and camera, the mobile microscopy apparatus comprising: an illumination module for illuminating a removable media with an illuminating light, an image acquisition optics for creating an image of the sample for acquisition by the camera of the mobile computing device, and a mounting frame assembly for detachably mounting the illumination module and the image acquisition optics to the mobile computing device and for holding the removable media in a predetermined position. In various implementations, the illumination module may provide either backside or frontside illumination of the removable media using the light generated by a light source of the mobile computing device. The mobile microscopy apparatus may operate in the microscope configuration, visible colormatic microarray configuration, and/or fluorescent microarray configuration.

Abstract: Various aspects as described herein are directed to apparatuses, methods, and systems including a sandwiched arrangement having a light-access port, a scanning mirror, an optics region, and a spacer. The spacer provides a light-directing optical region that separates the scanning mirror and the optics region, and includes a mirrored surface that reflects light between the light-access port and the optics region. Additionally, the optics region includes a curved-shaped window that provides a field of view by communicating beams of light between a target region. The optics region also includes a curved-shaped mirror having a surface that reflects light between the scanning mirror and the mirrored surface. Light beams, as conveyed between the light-access port and the curved-shaped window, are folded by being reflected off the scanning mirror, the curved-shaped mirror and the mirrored surface.

Abstract: Imagers and alignment methods for use by imagers imaging deoxyribonucleic acid (DNA) fragments on a flow cell are disclosed. The imagers capture intensity values at DNA fragment bead locations in tiles with each tile having a reference location in the flow cell. Flow cells may be aligned by obtaining a dark field image of each tile during a first imaging session, identifying dark field constellations of bead locations within two separate tiles during the first imaging session, identifying corresponding constellations during a second imaging session, altering the reference location of at least one tile during the second imaging session to correct for a linear offset in the corresponding constellations, and applying at least one correction factor for reading out intensity values from the imager for the bead locations in the flow cell to correct for an angular offset determined from offsets in the corresponding constellations.

Abstract: A partitioned aperture wavefront imaging system includes an imaging system comprising a partitioned aperture lens array positioned at the aperture plane or Fourier plane between the entrance plane and camera plane of an imaging system. The partitioned aperture lens array can include 2 or more off-axis lenses symmetrically distributed about an optical axis, and adapted to produce simultaneously at the camera plane at least two images of an object, or intermediate image of an object, presented at the entrance plane. Preferably, the partitioned aperture lens array includes from 3 to 5 off-axis lenses and produces 3 to 5 images at the camera plane from which phase and amplitude information about the light field can be determined. The partitioned aperture wavefront imaging system provides enough information about the light field presented at the entrance plane to enable reconstruction of the light field at other planes relative to the entrance plane.

Abstract: Referencing of image acquired in multiple rounds of imaging is disclosed. In certain implementations, a baseline round of images are acquired and registered to one another to establish a global transformation matrix. In a subsequent round of image acquisition, a limited number of field of view images are initially acquired and registered to the corresponding baseline images to solve for translation, rotation, and scale. The full set of baseline images is then acquired for the subsequent round and each image is pre-rotated and pre-scaled based on the transform determined for the subset of images. The pre-rotated, pre-scaled images are then registered using a translation-only transform.

Abstract: The present invention provides an inspection device including an imaging unit 16 for imaging an object to be inspected, a characteristics measurement unit 15 for measuring characteristics of the object to be inspected, an inspection information acquisition unit 11 for acquiring inspection information related to the object to be inspected, a condition determination unit 12 for determining measurement information related to a measurement condition of the object to be inspected corresponding to the inspection information, an imaging control unit 14 for controlling imaging by the imaging unit, and a measurement control unit 13 for controlling measurement by the characteristics measurement unit based on the measurement information.

Abstract: Provided is an information processing apparatus including: a connection connecting to a microscope including a stage having a disposition surface on which a target object can be placed, and an image picking-up section including an objective lens for picking up an image of the object, the microscope being movable in first and second axis directions orthogonal to an optical axis of the objective lens and orthogonal to each other and in a third axis direction along the optical axis; a calculator calculating, as an undulation-correcting value, a value for correcting a misalignment of a position in the third axis direction for each predetermined image-capturing range smaller than the disposition surface; a correcting-value storage storing the calculated value; and a corrector correcting a relative distance between the stage and the objective lens on the basis of the stored value for each image-capturing range.

Abstract: Embodiments of the present invention are directed to imaging technologies, and, in particular, to an imaging system that detects relatively weak signals, over time, and that uses the detected signals to determine the positions of signal emitters. Particular embodiments of the present invention are directed to methods and systems for imaging fluorophore-labeled samples in order to produce images of the sample at resolutions significantly greater than the diffraction-limited resolution associated with optical microscopy. Embodiments of the present invention employ overlapping-emitter-image disambiguation to allow data to be collected from densely arranged emitters, which significantly decreases the data-collection time for producing intermediate images as well as the number of intermediate images needed to computationally construct high-resolution final images. Additional embodiments of the present invention employ hierarchical image-processing techniques to further resolve and interpret disambiguated images.

Abstract: Unnecessary degradation of a light detector is prevented.