Abstract: A scanning microscope includes: a first scanning optical system that irradiates a specimen with light from a first light source via an objective lens to receive light from the specimen; and a second scanning optical system that irradiates the specimen with the light from the first light source or light from a second light source different from the first light source via the objective lens so as to cause the specimen to express a specific phenomenon. The second scanning optical system has a beam shaping optical system that shapes the light from the first light source or the light from the second light source such that a light convergence region on which the light from the first light source or the light from the second light source is collected via the objective lens satisfies a predetermined condition.

Abstract: Microscopy methods and apparatus in which one or more microfabricated optical elements (e.g., one or more Fresnel zone plates) operate as one or an array of objective lenses. A single object or a plurality of objects may be scanned in parallel. A single, low-numerical-aperture relay optic can be used with the one or more optical elements eliminating the need for one or more confocal pinhole apertures. When an array of optical elements is used, hundreds to thousands of objects can be imaged or inspected simultaneously onto a two-dimensional imaging device, such as a CCD array. The microfabricated optical elements can be readily configured for imaging with a solid immersion medium. Imaging resolutions on the order of one wavelength of the illumination source, and less, can be achieved.

Abstract: An illumination device (20) for a microscope (40) has a laser unit (24) that generates at least one broadband laser light pulse (30); light components (71, 72, 73, 74, 75, 76) of different wavelengths of said broadband laser light pulse (30) being offset in time from one another. A compensation unit (36) disposed in the path of the broadband laser light pulse (30) temporally offsets the light components (71, 72, 73, 74, 75, 76) of the broadband laser light pulse (30) in such a way that they exit the compensation unit (36) simultaneously or nearly simultaneously.

Abstract: Laser scanning microscope and method for the operation thereof having at least two detection channels which has at least one beamsplitter with a splitting of the sample light deviating from the 50:50 split and/or, with 50:50 split in the detection channels, has detectors with differently adjusted gain, or in at least one detection channel with equal light splitting has an additional light attenuator.

Abstract: A structured illuminating apparatus includes a branching unit branching an exit light flux from a light source into at least two branched light fluxes, an illuminating optical system making the two branched light fluxes to be respectively collected at mutually different positions on a pupil plane of an objective lens and making the two branched light fluxes to be interfered with each other to illuminate a specimen with an interference fringe of the two branched light fluxes, and an adjusting unit adjusting a height from an optical axis of the illuminating optical system to two collecting points formed on the pupil plane of the objective lens by the two branched light fluxes.

Abstract: Illuminating light is two-dimensionally scanned without changing the ability to focus illuminating light on a specimen. A microscope has a spatial light modulator for the wavefront of illuminating light from a light source; a scanner having two mirrors independently pivoted about two non-parallel axes; a relay optical system guiding the illuminating light, whose traveling direction has been changed by the scanner, to an objective optical system; and an adjusting unit that moves a wavefront modulation region of the modulator, in which an image is formed, in response to pivoting of the mirrors, such that an image at the pupil position of the objective optical system assuming that the mirrors are stopped is moved opposite to the direction of movement of the image relayed to the pupil position of the objective optical system assuming that the mirrors are pivoting while the image on the spatial light modulator is fixed.

Abstract: An ATR objective (1) for an IR microscope has a Cassegrain objective (2), an ATR crystal (7), a holding bar (8) to one end of which on the side of the sample, the ATR crystal (7) is mounted, a holding element (10), thin struts (9) which rigidly connect the holding bar (8) to the holding element (10) and intersect an optical path of the ATR objective (1) entering or exiting the Cassegrain objective (2) in such a fashion that they shade less than 10% of the beam cross-section of the optical path, and a motor drive (12) for axial movement of the holding element (10) relative to the sample position (3). The automated ATR objective thereby enables simple adjustment of operating modes and different contact pressures of the ATR crystal with respect to a sample (19).

Abstract: A system of analysis with the naked eye and by fluorescence of a field in an illuminated area comprising a periodically-excited first low-remanence white light illumination source; a second light source for exciting fluorescent elements located in said field, active at least during part of the time periods when the first source is off; and a fluorescence analysis device active during time periods when the first source is off and the second source is on.

Abstract: A microscope has an objective, a light source illuminating a sample over an illumination beam path, an arrangement producing a flat illumination pattern which is structured in both spatial directions on the sample, a surface detector detecting light coming over one picture beam path, an arrangement shifting the illumination pattern on the sample in one displacement direction, and a control unit taking one picture at a time of the light which was detected by the detector as phase picture in different positions of the pattern along the displacement direction and to computationally reconstruct from these phase pictures an overall picture of the illuminated sample region. The displacement direction is oblique to the main axes of symmetry of the illumination pattern and depending on the illumination pattern is chosen such that the number of phase pictures which is necessary for the picture reconstruction corresponds to the theoretically minimally required value.

Abstract: A microscope adapter unit disposed on an optical path of illumination light between a light source unit including a light source and a sample surface includes a first lens group having at least one lens and a second lens group having at least one lens. The first lens group converts the illumination light into roughly parallel luminous fluxes, and makes the illumination light enter the second lens group.

Abstract: An arrangement for use in illuminating a sample in SPIM microscopy includes an illumination objective configured to receive and focus a light strip or a quasi-light strip. The quasi-light strip is made up of a light bundle continuously moved back and forth in a light-strip plane. A deflection apparatus is configured to deflect the light strip or the quasi-light strip, after the light strip or the quasi-light strip has passed through the illumination objective, in such a way that the light strip or the quasi-light strip propagates at an angle different from zero degrees with respect to an optical axis of the illumination objective. The illumination objective and the deflection apparatus are arranged movably relative to one another.

Abstract: A microscope adapter unit disposed on an optical path of illumination light between a light source unit including a light source and a sample surface includes a first lens group having at least one lens and a second lens group having at least one lens. The first lens group converts the illumination light into roughly parallel luminous fluxes, and makes the illumination light enter the second lens group.

Abstract: A system, apparatus and method for using modular microscopes is disclosed. Connecting the housings of the individual microscope modules provide the structural framework of the modular microscope. Furthermore, the modular microscope can include specialized software, the distribution and use of which can be controlled using security keys or identifiers stored on one or more of the microscope modules. The security keys and identifiers can be based on calibration data associated with the physical, electrical, or optical properties of one of more of the modules. The illumination modules disclosed provide for selectable wavelengths and controllable levels of output illumination for both bright field and dark field illumination.

Abstract: A laser scanning microscope has an illumination beam path and a detection beam path. A beamsplitter is provided which reflects the illumination light in direction of the sample and transmits the detection light in direction of the detection arrangement. An additional beamsplitter is provided for reflecting the illumination light and for transmitting the detection light, this additional beamsplitter being arranged in the illumination beam path downstream of the first beamsplitter in the illumination direction, and this additional beamsplitter substantially transmits the illumination light reflected at the first beamsplitter and the detection light, but acquires a wavelength range substantially different from the first beamsplitter with respect to its reflectivity.

Abstract: An optical microscope includes a first mask that has transmission regions that are separated from one another for the simultaneous generation of a plurality of illumination light beams from illumination light, for example, a first scanning device for generating a scanning motion of the illumination light beams and a sample holder. The optical microscope also includes a second mask with transmission regions separated from one another, which transmission regions are smaller than the transmission regions of the first mask in order to clip the illumination light beams, such that, through the scanning motion of the first scanning device, each of the illumination light beams can be successively passed onto different transmission regions of the second mask, and a second scanning device is provided for generating a scanning motion between the clipped illumination light beams and the sample holder. A method for examining a microscopic sample is also provided.

Abstract: A microscope includes: a light source configured to generate light for irradiating a specimen; a microscope main body which supports the light source and on which an accommodation unit that accommodates the specimen is placed; a plurality of optical units, each of which is detachably installed in the microscope main body, configured to arranged on an optical path of the light, and configured to change optical characteristics of the light incident thereon; an operation input unit that includes a plurality of input units configured to respectively receive inputs of drive signals for driving optical units to be controlled among the plurality of optical units; and a control unit configured to allocate optical units to be respectively driven with the drive signals by the plurality of input units according to the plurality of optical units installed in the microscope main body.

Abstract: A super-resolution microscope comprises: an illumination optical system that condenses a first illumination light beam for exciting the molecule from a stable state to a first quantum state and a second illumination light beam for further transitioning the molecule onto a sample in a manner that the first and the second illumination light beams are partially overlapped; a scanning section that scans the sample by relatively displacing the first and the second illumination light beams and the sample; a detection section that detects an optical response signal emitted from the sample; and a phase plate that is arranged in the illumination optical system and has M surface areas for modulating the phase of the second illumination light beam, wherein the phase plate comprises a monolayer optical thin film with M surface areas formed on an optical substrate with a thickness that satisfies the predetermined conditional expression.

Abstract: A microscope and methods for obtaining a phase image of a substantially transparent specimen. Light collected from a specimen illuminated by a temporally incoherent source is diffracted into a first order and either the zeroth or first order is low-pass filtered in a Fourier transform plane before the orders are recombined at a focal plane detector. By low pass filtering the first order diffracted beam into a plurality of wavelengths, a spectrally- and spatially-resolved quantitative phase image of the specimen is obtained.

Abstract: A light source arrangement (101) for an illumination device of a medical-optical observation apparatus has an illumination light source (7) and an illumination optical unit (15) for illuminating an observation object (23) with illumination light from the illumination light source (7). The light source arrangement (101) has at least one luminescence emitter (3) as light source and an imaging optical unit (105) that generates an image (7) of the at least one luminescence emitter (3) with a defined magnification scale, which image forms the illumination light source for the illumination device.

Abstract: A transillumination device (150) for a microscope (100) comprises a flat panel light source (151), a diaphragm arrangement (152) arranged behind the flat panel light source (151) in the radiating direction (AR) that comprises two diaphragm elements movable relative to one another, at least one of the two diaphragm elements having a cutout, the two diaphragm elements defining, together with the at least one cutout, a diaphragm opening, wherein the dimensions of the diaphragm opening in two mutually perpendicular directions are determined by the position of the diaphragm elements relative to one another.

Abstract: A microscope (10) has an aperture arrangement (29) that, in order to limit the dimension of a light beam (41), comprises an aperture opening (37). The size of the aperture opening (37) is adjustable with the aid of a first aperture member (32) and a second aperture member (34). At least one of the two aperture members (32, 34) is movable relative to the other aperture member (32, 34). The aperture members (32, 34) are spaced apart from one another when the aperture opening (37) is closed.

Abstract: Test kits for assessing male fertility include a sample holder defining an object plane, a lens, and a two dimensional light sensor defining an image plane arranged along a common linear axis. The distance between the object plane and the image plane may be no more than 50 mm, and may be no more than 30 mm. A lens aperture may have an area of 1-10 mm2. The test kit may have a housing with a maximum linear dimension of no more than 100 mm. Processing circuitry may be provided that is configured to produce a sperm count and/or sperm motility measurements by processing image data from the two-dimensional light sensor.

Abstract: A method for SPIM microscopy, wherein the sample is moved continuously, and a plurality of images are taken at time intervals by means of a detection arrangement during the movement. The image capture duration or exposure time is dimensioned such that the movement path of the sample lies within a predetermined resolution range of the detection objective. The speed of the sample movement is determined and set by the image capture duration or exposure time and/or the distortion of the point spread function generated by the sample movement of the sample. The image blur is corrected computationally by the respective image capture duration and the movement speed. A sharp image is generated in this way. The actual optical section thickness of the light sheet is determined from the light sheet thickness, and the movement speed is determined therefrom and from user settings.

Abstract: A 4-Pi microscope for imaging a sample, comprising a first objective for focusing a first light beam on the sample at a spatial point one or more Digital Optical Phase Conjugation (DOPC) devices, wherein the DOPC devices include a sensor for detecting the first light beam that has been transmitted through the sample and inputted on the sensor; and a spatial light modulator (SLM) for outputting, in response to the first light beam detected by the sensor, a second light beam that is an optical phase conjugate of the first light beam; and a second objective positioned to transmit the first light beam to the sensor and focus the second light beam on the sample at the spatial point, so that the first light beam and the second light beam are counter-propagating and both focused to the spatial point.

Abstract: Embodied is a two-color, fiber-delivered picosecond source for coherent Raman scattering (CRS) imaging. A wavelength tunable picosecond pump is generated by nonlinear spectral compression of a prechirped femtosecond pulse from a mode-locked titanium:sapphire (Ti:S) laser. A 1064-nm picosecond Stokes pulse is generated by an all-fiber time-lens source (or suitable alternative source) that is synchronized to the Ti:S laser. The pump and Stokes beams are combined in an optical fiber coupler, which serves not only as the delivery fiber but also as the nonlinear medium for spectral compression of the femtosecond pulse. CRS imaging of mouse skin is performed to demonstrate the practicality of this source.

Abstract: Laser pulses are applied to surface plasmon resonant articles such as gold nanoparticles within a microscopy sample to generate a four-wave mixing signal that is detected as the output of the microscopy process.

Abstract: A light source and/or a deflector and/or the emitting end of an illuminating optical fiber is arranged in the rear focal plane of a lens for total internal reflection microscopy.

Abstract: The invention relates to an optical arrangement (20) and to a method of examining or processing an object (46). Here, a first laser pulse with a first central wavelength and a second laser pulse with a second central wavelength different from the first central wavelength are generated. Both pulses are superimposed in or on the object (46) such that multi-photon absorption takes place there with the involvement of at least one photon of the first laser pulse and at least one photon of the second laser pulse.

Abstract: A broad-spectrum, multiple wavelength illuminator comprises a luminescent body, and a plurality of semiconductor chips spaced apart from the luminescent body emitting light within one or more wavelength ranges towards the luminescent body, causing the luminescent body to emit light of one or more wavelength ranges. An optical element adjacent to the luminescent body collects light emitted by the luminescent body. An optical device collects light collected by the optical element. An aperture located between the optical element and the optical device passes the light emitted by the luminescent body along an optical axis, wherein light collected by the optical element and the optical device and passed by the aperture forms a beam of light illuminating a target. Alternatively, instead of being spaced apart from the chips, the luminescent body may be a layer adjacent to the chips.

Abstract: In accordance with one embodiment of the present invention an apparatus for a low numerical aperture exclusion imaging apparatus is provided. The apparatus may include an electromagnetic illumination source for illuminating a portion of a specimen; and for collecting an image created by the electromagnetic radiation an objective lens optically coupled to the electromagnetic illuminated portion of the specimen. The apparatus also includes an optical blocking plate disposed between the objective lens and a focusing lens. The optical blocking plate is positioned to substantially block undesired electromagnetic radiation from image sources distally aligned in the same optical axis as the specimen. This invention is enhances narrow depth of field characteristics in imaging. It also enhances discreet imaging in a narrow focus field by eliminating some or most of the light which contributes to wide depth of field focus. This is useful for optical sectioning ranging from microscopy to photography.

Abstract: The invention relates to an illuminating device for a microscope with an illumination magazine comprising a plurality of light emitting units. A mechanical illuminator changer can change the light emitting unit currently active in the operative position. A filter magazine having a plurality of filter units is present, wherein a mechanical filter changer for changing the filter unit currently in the active operative position is associated with the filter magazine. At least one mechanical coupling component is provided for cooperation with the filter changer and the illuminator and for uniquely assigning each filter unit to a specific light emitting unit.

Abstract: A stereomicroscope fluorescence system has a light source, a light guide, a focusing lens assembly, a stereomicroscope with two eyepiece receivers, and two eyepiece assemblies. The light guide is operably attached to receive light from the light source to transmit the light to the focusing lens assembly. The focusing lens assembly includes an excitation filter coated on the focusing lens. The eyepiece assemblies each have a barrier filter and are adapted to be mounted on the eyepiece receivers.

Abstract: A microscopy system and method allow observing a fluorescent substance accumulated in a tissue. The tissue can be observed at a same time both with visible light and with fluorescent light. It is possible to observe a series of previously recorded fluorescent light images in superposition with the visible light images. An end of the series of images may be automatically determined. A thermal protective filter may be inserted into a beam path of an illuminating system at such automatically determined end of the series. Further, the fluorescent light image may be analyzed for identifying a coherent fluorescent portion thereof. A representation of a periphery line of the coherent portion may be generated, and depths profile data may be obtained only from the coherent portion. An illuminating light beam for exciting the fluorescence may be modulated for improving a contrast of fluorescent images.

Abstract: A microscope adapter unit disposed on an optical path of illumination light between a light source unit including a light source and a sample surface includes a first lens group having at least one lens and a second lens group having at least one lens. The first lens group converts the illumination light into roughly parallel luminous fluxes, and makes the illumination light enter the second lens group.

Abstract: A confocal fluorescence microscope of the present invention consists of: a light source unit broadly comprising one or more short wavelength laser beams; a lens unit which converts parallel light, emitted from a light source, into linear light having an appropriate size; a multi-color mirror which reflects the light source and enables fluorescence to transmit so as to separate the light source and the fluorescence; a scan mirror which radiates the light source over a wide area and scatters the fluorescence over a large-area camera; a microscope unit which radiates the incident light source to a target object, collects the fluorescence emitted from the target, and outputs the collected fluorescence; and a detecting unit which removes the background of the outputted fluorescent signal and observes the outputted fluorescent signal.

Abstract: An adjustable stage mount includes a housing having a base defining a hole and an adjustable stage including a ball joint extension that rotatably engages the hole and is rotatably securable about the x-, y-, and z-axes. An adjustable indenter mount includes a housing defining a hole and an adjustable indenter including a ball joint extension that rotatably engages the hole and is rotatably securable about the x-, y-, and z-axes. A collision protection switch includes a first plate having three pairs of electrically conductive spaced apart pins wired to a voltage source in an open circuit, and a second plate having three electrically conductive balls. A spring pulls the first and second plates together causing the three balls to complete the circuit. A sufficient force against the second plate causes a ball to disengage and open the circuit. A two-objective microscope includes two parallel objectives, upper and lower light sources, three half-mirrors, and a camera.

Abstract: A method for the optical detection of an illuminated specimen, wherein the illuminating light impinges in a spatially structured manner in at least one plane on the specimen and several images of the specimen are acquired by a detector in different positions of the structure on the specimen. An optical sectional image and/or an image with enhanced resolution is then calculated. The method includes generating a diffraction pattern in the direction of the specimen in or near the pupil of the objective lens or in a plane conjugate to the pupil. A phase plate with regions of varying phase delays is dedicated to the diffraction pattern in or near the pupil of the objective lens or in a plane conjugate to said pupil, and different phase angles of the illuminating light are set.

Abstract: The present invention relates to a capillary-based cell and tissue acquisition system that integrates the capillary approach with a microscope manipulator to collect and sort cells of interest. Cells of interest are determined by using a laser beam focus to identify the initial contact between the capillary and the cells.

Abstract: A laser scanning microscope (LSM) consisting of at least one light source from which an illumination beam path extends in the direction of a sample, at least one detection beam path for transmitting sample light to a detector array, a first pinhole for confocal filtering in front of the detector array, a scanner for causing a relative motion between the illumination light and the sample in at least one direction, and a microscope lens. For illuminating a sample, at least two illumination beams, which the microscope lens focuses as illumination points in a sample plane, are generated in the illumination beam path. The laser scanning microscope is characterized in that in addition to the preferably adjustable, slit-shaped first pinhole, a second, preferably adjustable, slit-shaped pinhole is arranged downstream of the first pinhole so as to create optically conjugate beams arranged between the first and the second pinhole.

Abstract: Fluorescence is generated from an irradiated point on an inspection surface of a sample and the fluorescence is collected by an objective lens. Here, because of the magnification chromatic aberration of the objective lens, the fluorescence going out from the objective lens travels along a path shifted from the irradiation light and changed substantially into a non-scan light by a galvano-scanner. The fluorescence passes through a dichroic mirror into a plane-parallel plate after light of unnecessary wavelength is removed by a filter. The plane-parallel plate is driven in synchronization with the galvano-scanner by a computer and corrects the shift and inclination of the optical axis generated by the magnification chromatic aberration of the objective lens. Then, the fluorescence forms an image of the irradiation point of the inspection surface of the sample on a pin hole of a pin hole plate by using a collective lens.

Abstract: A set of filters for fluorescence observation comprises an illumination light filter and an observation light filter, wherein the following is holds: ? S ? T L ? ( r -> ) · T O ? ( r -> ) · r -> · ? r ? S ? T L ? ( r -> ) · T O ? ( r -> ) · ? r = R -> ? ? and ( 3 ) ? W -> - R -> ? ? 0.2 ; ( 4 ) wherein: ? designates the wavelength, TL(?) is the transmission characteristic of the illumination light filter, TO(?) is the transmission characteristic of the observation light filter, and A1, A2 are numbers between 0 and 1, {right arrow over (r)} is a coordinate in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space, S is a line called the spectral locus in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space, and {right arrow over (W)} is the white point in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space.

Abstract: A microscope including an illumination device for a light sheet having an approximately planar extension along an illumination axis of an illumination beam path with a transverse axis to the illumination axis. Light emitted from the sample region on axis of detection the illumination axis and the axis of detection as well as the transverse axis and the axis of detection being oriented relative at an non-zero angle. The illumination device includes structure deflecting light to different beam path and thus produces an additional sheet of light, together illuminating the sample region on common illumination axis, and with switches between beam paths. Detection device has a detection lens system for light from by the sample region. The switches include a rapidly switching element with a time <10 ms, a predetermined integration of the surface detector synchronized so the sample region is illuminated twice during integration.

Abstract: Method for actuation control of a microscope, in particular of a Laser Scanning Microscope, in which, at least one first illumination light, preferably moving at least in one direction, as well as at least one second illumination light moving at least in one direction, illuminate a sample through a beam combiner, a detection of the light coming from the sample takes place, whereby, at least one part of the illumination light is generated through the splitting of the light from a common illuminating unit, characterized in that, by means of a common control unit, a controlled splitting into the first and the second illumination light takes place, in which the intensity of the first illuminating light, specified by the user or specified automatically, is assigned a higher priority (is prioritized) compared to the specified value for the second illumination light, and an adjustment for the second illumination light takes place until a maximum value is obtained, which is determined by the value specified for the f

Abstract: An illuminator for a transmitted light microscope for producing stereo viewing as well as motion parallax 3D perception, using a generally pyramid-shaped mirror having a plurality of facets and light source adjacent a plurality of the facets and a computer-driven electronic circuit for controlling the on/off status of said light sources.

Abstract: The invention relates to a laser scanning microscope (LSM), consisting of at least one light source, from which an illumination beam path in the direction of a sample originates, at least one detection beam path for passing sample light, preferably fluorescence light, onto a detector arrangement, it main colour separator for separating the illumination and detection beam paths, a microlens array for generating a light source grid composed of at least two light sources, a scanner for generating a relative movement between the illumination light and the sample in at least one direction, and a microscope objective, wherein the lens array is arranged in at common part of illumination and detection beam paths.

Abstract: An optical member and a microscope that allow acquiring brighter and sharper images when fluorescent observation is performed while stimulating a sample with light. Illumination light from a laser unit is split into stimulation light and excitation light by a dichroic mirror. In other words, half of the illumination light is transmitted through the dichroic mirror and becomes the stimulation light, and half of the illumination light is reflected by the dichroic mirror and becomes the excitation light. Half of the excitation light is reflected by a dichroic mirror and is irradiated onto a sample, and half of the stimulation light transmits through the dichroic mirror and is irradiated onto the sample. Fluorescence generated from the sample is totally reflected by the dichroic mirror and the dichroic mirror, and is received by a photodetector.

Abstract: The embodiments of this invention use a multi-point scanning geometry. This design maintains the high frame rate of the slit-scan system and still allows both grayscale and multi-spectral imaging. In a confocal configuration, the multi-point scanning system's confocal performance is close to that of a single point scan system and is expected to yield improved depth imaging when compared to a slit-scan system, faster imaging than a point scan system, and the capability for multi-spectral imaging not readily achievable in a Nipkow disk based confocal system.

Abstract: The present invention provides a slit-scan multi-wavelength confocal lens module, utilizing at least two lenses having chromatic aberration for splitting a broadband light into continuously linear spectral lights having different focal length respectively. The present invention utilizes the confocal lens module employing slit-scan confocal principle and chromatic dispersion techniques and the confocal microscopy with optical sectioning ability and high resolution in spectral dispersion to establish a confocal microscopy method and system with long DOF and high resolution, capable of modulating a broadband light to produce the axial chromatic dispersion and focus on different depths toward an object's surface for obtaining the reflected light spectrum from the surface.

Abstract: A dome illumination for a microscope and a microscope are disclosed. At least one objective lens carries a dome at a free end, wherein the free end of the objective lens is facing a surface of the object. At least one light source is arranged such that an illumination is provided to the dome when the objective lens is positioned in an optical axis or working position of the microscope.

Abstract: An endoscope light source unit comprising: a first light source section emitting a first illumination light composed of white light; a second light source section emitting a second illumination light orthogonally to a direction in which the first illumination light travels; a light combining member transmitting the first illumination light through a transmission section and, at the same time, reflecting the second illumination light by means of a reflection section to form a combined light so that a beam of the second illumination light may be located in a central part of a beam of the first illumination light; a shaping lens for modifying the beam of the second illumination light emitted; and a condenser lens for converging the combined light.